[Research progress on the role of calcium ion transport in dental biomineralization].

Tooth development is a complex biomineralization process formed through the interaction between epithelial tissue and mesenchymal tissue. The Ca transport channel is the intrinsic regulatory mechanism of this process, which regulates the concentration of Ca inside and outside the cell in time and space, thereby affecting the biological functions of various cells and the growth of extracellular hydroxyapatite. This article reviews the researches on the effects of Ca transport in tooth biomineralization and corresponding cell development in recent years, with the aim of providing ideas and basis for the prevention of tooth developmental defects, assisting in early clinical diagnosis, and improving treatment methods.

Extended Reality Technology for Alleviating Adult Dental Anxiety: A Systematic Review and Meta-analysis of Randomized Controlled Trials.

Objectives: To assess the effectiveness of extended reality (XR) technology in reducing dental anxiety in adults, through a systematic review and meta-analysis.

Methods: Comprehensive electronic and manual searches were conducted to identify randomized controlled trials published up to November 23, 2024, with no restrictions on publication year or language. Key outcomes included changes in anxiety and pain levels and cardiovascular indicators, such as blood pressure, heart rate, and oxygen saturation (SpO2).

A survey of attitudes towards the curriculum for clinical medicine postgraduates pursuing professional master's degrees: perspectives of supervisors and students.

Background: Based on the recommendation of the Ministry of Education in China to differentiate between "academic" and "professional" degrees, medical schools offer both professional and academic degrees for postgraduates. In China, clinical postgraduates who are pursuing a professional master's degree also participate in standardised residency training (SRT). However, little attention has been given to feedback from students and supervisors regarding postgraduate curricula.

A shielded cascade of targeted nanocarriers spanning multiple microenvironmental barriers for inflammatory disease therapy.

Background: The multi-biological barriers present in the inflammatory microenvironment severely limit the targeted aggregation of anti-inflammatory drugs in the lesion area. However, conventional responsive drug carriers inevitably come into contact with several pro-responsive stimulatory mediators simultaneously, leading to premature drug release and loss of most therapeutic effects. Breaking through the multi-level barriers of the inflammatory microenvironment is essential to improve the enrichment and bioavailability of drugs.

[Application of poly ether ether ketone localization marker combined with mixed reality technology in vessel localization of anterolateral thigh perforator flap].

Objective: To compare the effectiveness of poly ether ether ketone (PEEK) localization marker combined with mixed reality technology versus color doppler ultrasound guidance for the vessel localization of anterolateral thigh perforator flap.

Methods: A retrospective analysis was conducted on 40 patients with tissue defects after oral cancer resection who underwent repair using the anterolateral thigh perforator flap between January 2022 and June 2023. According to the different intraoperative positioning methods of the anterolateral thigh perforator flap, they were randomly divided into PEEK group [using PEEK localization marker combined with mixed reality technology based on CT angiography (CTA) data] and color ultrasound group (using color ultrasound guidance), with 20 cases in each group.

The regulatory role of BMP9 on lipopolysaccharide-induced matrix metalloproteinases in human stem cells from the apical papilla.

Objective: The aim of this study was to investigate changes in the expression of members of the matrix metalloproteinases (MMPs) family in response to lipopolysaccharide (LPS) stimulation and to investigate the regulatory effects of BMP9 on MMPs.

Design: The extracted human stem cells from the apical papilla (hSCAPs) were identified by flow cytometry, Alizarin Red staining, Oil Red O staining, and alkaline phosphatase staining. The appropriate LPS concentration for inducing inflammation in hSCAPs was determined using real-time quantitative PCR (RT-qPCR) and Cell Counting Kit-8 (CCK-8) assays.

A Bibliometric Analysis of Macrophage Research Associated with Periodontitis Over the Past Two Decades.

Macrophages are vital for regulating periodontal health, and numerous studies have extensively investigated their role in periodontitis progression. This study employs bibliometric analysis to identify research trends and hotspots related to macrophages in periodontitis from 2004 to 2024, thereby guiding future investigations and exploring potential clinical applications. Literature retrieval and dataset export were conducted via the Web of Science Core Collection database.

RANK IVVY motif plays crucial roles in osteoclastogenesis.

RANKL and its receptor RANK play a vital role in osteoclastogenesis. RANK primarily recruits TRAFs to promote osteoclastogenesis but also contains an TRAF-independent motif (IVVY), which mediates osteoclast lineage commitment in vitro. Here, we have developed knockin mice in which inactivating mutations are introduced in the IVVY motif (IVVY to IVAF).

Efficacy of radiotherapy combined with atezolizumab or docetaxel in patients with previously treated NSCLC.

Radiotherapy showed synergy with immunotherapy, yet the comparative effectiveness of combining immunotherapy (iRT) or chemotherapy (CRT) after platinum therapy failure in advanced non-small cell lung cancer (NSCLC) remains unexplored. We analyzed 163 patients (iRT:  = 120 vs. CRT:  = 43) eligible for combination radiotherapy.

Skeletal muscle-derived musclin attenuates glycolysis, oxidative stress, and pulmonary hypertension through the NPR3/AKT/mTORC1 pathway.

Exercise ameliorates pulmonary hypertension (PH) progression. However, the underlying mechanisms are largely unclear. Musclin is an exercise-responsive myokine that exerts protective effects on cardiovascular diseases.

Predicting the decline of physical function among the older adults in China: A cohort study based on China longitudinal health and longevity survey (CLHLS).

Background: As the arrival of healthy aging, maintaining physical function (PF) in older adults is crucial for their health, so it is necessary to detect the decline of PF among them and take intervention measures.

Methods: We construct eight machine learning models to predict declines of PF in this study. The performance of the models was tested by Area Under Curve (AUC), sensitivity, specificity, accuracy, precision-recall (PR) curve and calibration degree.

Prospective study on the localization of anterolateral thigh perforator vessel based on mixed reality and artificial algorithm.

Objectives: This paper aims to construct a system integrating mixed reality technology with artificial algorithm and to evaluate its effectiveness in vascular localization during anterolateral thigh perforator flap surgery to provide new insights for clinical practice.

Methods: Twenty patients undergoing anterolateral thigh perforator flap repair were selected. After attaching positioning devices on the lower limb, CT angiography (CTA) scans were performed.

Establishment of immortalized rabbit bone marrow mesenchymal stem cells and a preliminary study of their osteogenic differentiation capability.

Background: A stable and standardized source of mesenchymal stem cells is a prerequisite for bone repair tissue engineering research and application. We aimed to establish a stable cell line of bone marrow mesenchymal stem cells from New Zealand rabbits and explore their osteogenic differentiation capacity.

Methods: Primary rabbit bone marrow mesenchymal stem cells (RBMSCs) were isolated and immortalized via retroviral expression of SV40 Large T antigen (LTA).

Treatment motivation, psychosocial impact, and perfectionism in children and adult orthodontic patients: A cross-sectional study.

Introduction: With a shift in orthodontics from a more traditional biomedical model toward a more biopsychosocial model, orthodontists must understand their patients' psychological condition. This study aimed to investigate treatment motivation, psychosocial impact from malocclusion, and perfectionism in children and adult orthodontic patients and to examine the relationships among these various factors.

Methods: A total of 193 participants (114 children and 79 adults) were included.

Belimumab safety in adult and paediatric Chinese patients with systemic lupus erythematosus: A Phase 4, multicentre, observational study.

Objective: Although belimumab has been widely used in patients with systemic lupus erythematosus (SLE) globally, real-world safety data among Chinese patients are limited, particularly for children. This study assessed the safety and tolerability of belimumab in adult and paediatric patients with SLE in China in real-world clinical practice.

Methods: This Phase 4, multicentre, prospective, observational study enrolled patients prescribed intravenous belimumab by their physicians in tertiary hospitals, independent of a clinical study, during routine clinical visits between May 2021 and May 2022.

Potential pro-tumour cytokine in oral squamous cellular carcinoma: IL37.

Inflammation and immunosuppression are important features of tumours, including oral squamous cellular carcinoma (OSCC). Interleukin 37 (IL37), a cytokine known for the ability to suppress inflammation and immunity, shows two seemingly contradictory functions in tumours. This study aims to investigate the mechanism that regulates IL37 and its role in OSCC progression.

Multivalent acetylated-sialic acid as recognition elements for the electrochemical sensing of viral antigens.

Electrochemical biosensors hold great promise for the rapid screening of viral infectious diseases. However, the recognition elements of these biosensors are typically limited to antibodies, aptamers, and molecularly imprinted polymers. In this study, acetylated sialic acids were explored as recognition elements because they serve as natural viral receptors expressed on host cells.

Synthetic nanointerfacial bioengineering of Ti implants: on-demand regulation of implant-bone interactions for enhancing osseointegration.

Titanium and its alloys are the most commonly used biometals for developing orthopedic implants to treat various forms of bone fractures and defects, but their clinical performance is still challenged by the unfavorable mechanical and biological interactions at the implant-tissue interface, which substantially impede bone healing at the defects and reduce the quality of regenerated bones. Moreover, the impaired osteogenesis capacity of patients under certain pathological conditions such as diabetes and osteoporosis may further impair the osseointegration of Ti-based implants and increase the risk of treatment failure. To address these issues, various modification strategies have been developed to regulate the implant-bone interactions for improving bone growth and remodeling .

A Prediction Model for External Root Resorption of the Second Molars Associated With Third Molars.

Objectives: The aim of this study is to investigate risk factors for external root resorption (ERR) of second molars (M2) associated with impacted third molars (M3), and to develop a prediction model that can offer dentists a reliable and efficient tool for predicting the likelihood of ERR.

Methods: A total of 798 patients with 2156 impacted third molars were collected from three centres between 1 December 2018 and 15 December 2018. ERR was identified by cone beam computed tomography examinations.

CLEC14A facilitates angiogenesis and alleviates inflammation in diabetic wound healing.

Background: Delayed wound healing is a serious complication of diabetic wounds, posing a significant challenge to the treatment of patients with diabetes. Diabetic wound healing is a complex dynamic process involving angiogenesis and inflammatory responses. Currently, there are limited targeted therapies to promote diabetic wound healing.