Rare occurrence of upper-limb ischaemia necessitating transhumeral amputation following axillary arterial line placement.

Arterial cannulation, commonly performed in the radial artery, is a widely used method for continuous blood pressure monitoring. Occasionally, the axillary artery is used as an alternate site of cannulation. However, complications like occlusion can lead to adverse events and severe outcomes.

Conceptualizing and Measuring Systemic Racism.

This article provides a guide for rigorous, theory-driven measurement approaches, proposing best practices for the scientific study of systemic racism in health research. We argue that the analytical crux of measuring systemic racism-a complex, interconnected, and dynamic system-lies in operationalizing the collective logics, properties, and mechanisms that undergird racial inequities. Misalignment between measurement tools and these foundational features undermines research validity, as incongruent measures distort findings and obscure systemic racism's true impacts.

Mass Spectrometry-Based Applications of Spheroids in Cancer Biology.

The use of cell culture techniques to model human disease is an indispensable tool that has helped improve the health and well-being of the world. Monolayer cultures have most often been used for biomedical research, although not accurately recapitulating an in vivo human tumor. Tumor spheroids are a form of three-dimensional cell culture that better mimics an avascularized human tumor through their cell-cell contacts in all directions, development of various chemical gradients, and distinct populations of cells found within the spheroid.

Untargeted Characterization and Biological Activity of Amazonian Aqueous Stem Bark Extracts by Liquid and Gas Chromatography-Mass Spectrometry.

Introduction: Aqueous stem bark extracts of Aspidosperma rigidum Rusby, Couroupita guianensis Aubl., Monteverdia laevis (Reissek) Biral, and Protium sagotianum Marchand have been reported as traditional remedies in several countries of the Amazonian region. Despite previous research, further investigation to characterize secondary metabolites and the biological activity of extracts is needed to derive potential applications.

Distinct muscle growth and strength adaptations after preacher and incline biceps curl.

We compared performing preacher and incline biceps curls on changes in elbow flexors muscle size and strength. This was a between-group repeated measures randomized trial. Sixty-three young women performed preacher biceps curl (PC, n = 30) or incline biceps curl (IC, n = 33) for 8 weeks, twice a week.

Educational Strategies for Teaching Metabolic Profiles Across Three Endurance Training Zones.

This article explores an innovative educational approach using a metabolic board designed to enhance understanding of muscle metabolism across three endurance training zones: Z1 (light intensity), Z2 (moderate intensity), and Z3 (intense/severe intensity). The aerobic threshold marks the transition from light to moderate domains, and the anaerobic threshold separates moderate from intense domains, with both thresholds adapting to training. Exercises within each training zone elicit specific adaptive responses through distinct signaling pathways, but the metabolic profile induced remains relatively constant across these intensity domains.

Patches containing quercetin microcapsules to ameliorate dermal herpes simplex virus injuries in mice.

This study aimed to develop patches containing quercetin-loaded microcapsules and to evaluate their in vitro and in vivo safety and efficacy in preclinical surveys. A set of in vitro experiments evidenced the virucidal activity of quercetin against the HSV-1-KOS (sensitive to acyclovir) and HSV-1-AR (resistant to acyclovir) strains, with improved outcomes upon the first. The patches presented a homogeneous aspect, were easily handled, had a suitable bioadhesion, and possessed mechanical properties of soft and weak material, besides a pH compatible with human skin.

Adsorption of sulfamethoxazole in an aqueous environment onto a novel magnetic sporopollenin-cellulose triacetate.

Antibiotics are emerging environmental contaminants posing critical health risks due to their tendency to concentrate in living things and eventually infiltrate the human body. Sulfamethoxazole (SMZ) is among the commonly detected antibiotics in wastewater requiring effective removal approach. A sustainable, thermally stable and easily separable magnetic sporopollenin-cellulose triacetate (Msp-CTA) was developed via a simple step synthesis for eliminating SMZ from aqueous solution.

Transcriptomic analysis reveals suppression of steroidogenic acute regulatory protein in gender-specific differences in Alzheimer's disease.

Alzheimer's disease (AD)-related dementia preferentially impacts two-thirds of women and one-third of men. The steroidogenic acute regulatory (StAR) protein mediates the biosynthesis of neurosteroids that sustain diverse neuronal activities. Aging, involving neurosteroidal imbalance, is the predominant risk factor for AD causing dementia.

Responsiveness to cold snaps by turtle embryos depends on exposure timing and duration.

Characterizing how organisms respond to transient temperatures may further our understanding of their susceptibility to climate change. Past studies in the freshwater turtle, , have demonstrated that the timing and duration of heat waves can have major implications for the response of genes involved in gonadal development and the production of female hatchlings. Yet, no study has considered how the response of these genes to transient cold snap exposure may affect gonadal development and the production of males.

Lack of thermal acclimation in multiple indices of climate vulnerability in bumblebees.

Indices of climate vulnerability are used to predict species' vulnerability to climate change based on intrinsic physiological traits, such as thermal tolerance, thermal sensitivity and thermal acclimation, but rarely is the consistency among indices evaluated simultaneously. We compared the thermal physiology of queen bumblebees between a species experiencing local declines () and a species exhibiting continent-wide increases (). We conducted a multi-week acclimation experiment under simulated climate warming to measure critical thermal maximum (CT), critical thermal minimum (CT), the thermal sensitivity of metabolic rate and water loss rate and acclimation in each of these traits.

Parallel genetic adaptation amid a background of changing effective population sizes in divergent yellow perch () populations.

Aquatic ecosystems are highly dynamic environments vulnerable to natural and anthropogenic disturbances. High-economic-value fisheries are one of many ecosystem services affected by these disturbances, and it is critical to accurately characterize the genetic diversity and effective population sizes of valuable fish stocks through time. We used genome-wide data to reconstruct the demographic histories of economically important yellow perch () populations.

Enabling tumor-specific drug delivery by targeting the Warburg effect of cancer.

Metabolic reprogramming of tumor cells is an emerging hallmark of cancer. Among all the changes in cancer metabolism, increased glucose uptake and the accumulation of lactate under normoxic conditions (the "Warburg effect") is a common feature of cancer cells. In this study, we develop a lactate-responsive drug delivery platform by targeting the Warburg effect.

Barriers to Nursing School Admissions for Students With Physical or Sensory Disabilities: Do Essential Abilities Requirements Align With Licensure Standards and Legal Precedence?

Aim: The aim of the study was to examine sensory and motor essential abilities required by nursing boards and assess their inclusion in prelicensure nursing program admissions.

Background: The nursing workforce should mirror diverse patient populations for better concordance; however, essential abilities restrictions hinder admissions for students with disabilities.

Method: Requirements for licensure were collected from state boards of nursing and compared with essential abilities admissions requirements from 58 prelicensure programs.

When Leaders Don't Walk the Walk: A National Survey of Academic Nurse Leader Perceptions of Staff Burnout.

Aim: This survey explored nurse leaders' impressions of burnout on college/school of nursing (CON/SON) administrative staff and leadership-facilitated strategies used to promote resilience building/mitigate burnout.

Background: Administrative staff are foundational to the success of a university's CON/SON, yet few studies have explored the impact of burnout in this group.

Method: Cross-sectional survey distributed to associate dean and business officer attendees of the 2022 American Association of Colleges of Nursing, Business Officers of Nursing Schools meeting (summer 2022) (n = 64).

Structural and Dynamic Heterogeneity of Deep Eutectic Solvents Composed of Choline Chloride and Ortho-Phenol Derivatives.

Structural, thermal, and dynamic properties of four deep eutectic solvents comprising choline chloride paired with phenolic derivative hydrogen-bond donors were probed using experiments and molecular simulations. The hydrogen-bond donors include phenol, catechol, -chlorophenol, and o-cresol, in a 3:1 mixture with the hydrogen-bond acceptor choline chloride. Density, viscosity, and pulsed-field gradient NMR diffusivity measurements were conducted over a range of temperatures.

Relationships between Patient-Reported Outcomes and Predictors of Second ACL Injuries during Unanticipated Jump Landings.

Reactive and external visual-cognitive demands are prevalent in sport and likely contribute to ACL injury scenarios. However, these demands are absent in common return-to-sport assessments. This disconnect leaves a blind spot for determining when an athlete can return to sport with mitigated re-injury risk.

Limb Underloading in Walking Transmits Less Dynamic Knee Joint Contact Forces after Anterior Cruciate Ligament Reconstruction.

Introduction: Individuals with anterior cruciate ligament reconstruction (ACLR) often walk with a less dynamic vertical ground reaction force (vGRF), exemplified by a reduced first peak vGRF and elevated midstance vGRF compared to uninjured controls. However, the mechanism by which altered limb loading affects actual tibial plateau contact forces during walking remains unclear.

Methods: Our purpose was to use musculoskeletal simulation to evaluate the effects of first peak vertical ground reaction force (vGRF) biofeedback on bilateral tibiofemoral contact forces relevant to the development of post-traumatic osteoarthritis (OA) in 20 individuals with ACLR.

Contextualizing the Upper Paleolithic of the Armenian Highlands: New data from Solak-1, central Armenia.

As a potential corridor connecting Southwest Asia with western and northern Europe, the Armenian Highlands and southern Caucasus hold great potential for increasing our understanding of Upper Paleolithic behavioral and cultural variability. However, given the dearth of Upper Paleolithic sites, we lack the data necessary to answer basic questions regarding the timing and nature of the Upper Paleolithic in this region. Solak-1 is an open-air site located along the upper Hrazdan Valley (1635 m above sea level) in central Armenia.

A combination treatment with a water extract from Euglena gracilis and anti-PD-1 antibody strongly inhibits growth of lung cancer in mice through stimulating tumor-infiltrating lymphocytes.

Here, we investigated the relationship between the attenuation of lung cancer growth due to oral administration of Euglena gracilis water extract (EWE) and T cell stimulation. Orally administered EWE was revealed to increase PD-1 and PD-L1 mRNA and proteins primarily in tumor-infiltrating lymphocytes (TILs), which was correlated with a significant decrease in the tumor weights in mice. A combination treatment with EWE and anti-PD-1 antibody significantly decreased the growth of murine lung tumors more than treatment with either alone by increasing the number of TILs and attenuating T cell exhaustion.

A Single-Institution Analysis of Surveillance Practice for Low-Risk Neuroblastic Tumors.

Introduction: Currently, few prospective guidelines exist for the surveillance of children with low-risk neuroblastic tumors (LRNBTs), including ganglioneuroma or ganglioneuroblastoma intermixed. This study aims to describe our institutional approach to LRNBT surveillance following surgical resection or nonoperative management. We hypothesize that length of surveillance can be reduced due to low recurrence risk.

Harnessing plant growth-promoting bacteria (Herbaspirillum seropedicae) from an optimal mineral nitrogen supply: A study on improving nitrogen use efficiency in marandu palisadegrass.

Increasing nitrogen use efficiency (NUE) remains a crucial topic in contemporary agriculture. Inoculation with endophytic diazotrophic bacteria offers a potential solution, but the results vary with the N-fertilization regime. Here, we examined the efficacy of inoculation with Herbaspirillum seropedicae strain HRC54 in enhancing NUE and promoting the growth of Marandu palisadegrass with varying levels of N-urea (0, 25, 50, and 100 mg N kg soil⁻).

Diagnostic and prognostic perspectives of fabry disease via fiber evanescent wave spectroscopy advanced by machine learning.

Fabry disease (FD) is a rare disorder resulting from a genetic mutation characterized by the accumulation of sphingolipids in various cells throughout the human body, leading to progressive and irreversible organ damage, particularly in males. Genetically-determined deficiency or reduced activity of the enzyme (alpha - Galactosidase; α-Gal) leads to the accumulation of sphingolipids in the lysosomes of various cell types, including the heart, kidneys, skin, eyes, central nervous system, and digestive system, triggering damage, leading to the failure of vital organs, and resulting in progressive disability and premature death. FD diagnostics currently depend on costly and time-intensive genetic tests and enzymatic analysis, often leading to delayed or inaccurate diagnoses, which contribute to rapid disease progression.

Implementing a strength-based adverse childhood experiences prevention program for predominantly Indigenous families: A mixed-method process evaluation.

Background: Given high rates of adverse childhood experiences (ACEs) among Indigenous youth, it is critical to develop and evaluate strategies to prevent these experiences; one part of evaluation is process evaluation, including analysis of fidelity, attendance and barriers to attendance, contamination, and program acceptability.

Objective: To present a process evaluation of Tiwahe Wicagwicayapi, a strengths-based, family-based program for predominantly Indigenous youth (ages 10 to 14) and their caregivers. The program aimed to prevent ACEs including child abuse and neglect.