Inactivating and damaging properties of the disinfectant "MultiDez" when exposed to bacteria and spores.

Disinfectants play a crucial role in controlling the spread of infectious diseases caused by bacteria and spore-forming organisms. Bacteria and spores can persist on surfaces and in the environment for extended periods, posing a significant risk to public health. Disinfectants are designed to inactivate or kill these microorganisms by disrupting their cellular structures and functions.

Antibacterial Composite Material Based on Polyhydroxybutyrate and Zn-Doped Brushite Cement.

A composite material based on electrospinning printed polyhydroxybutyrate fibers impregnated with brushite cement containing Zn substitution was developed for bone implant applications. Powder X-ray Diffraction (PXRD), Fourier Transform Infrared Spectroscopy and Scanning Electron Microscopy were applied for materials characterization. Soaking the composite in Ringer's solution led to the transformation of brushite into apatite phase, accompanied by the morphology changes of the material.

In Vitro Properties of Manganese-Substituted Tricalcium Phosphate Coatings for Titanium Biomedical Implants Deposited by Arc Plasma.

Bioactive manganese (Mn)-doped ceramic coatings for intraosseous titanium (Ti) implants are developed. Arc plasma deposition procedure is used for coatings preparation. X-ray Diffraction, Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy, and Electron Paramagnetic Resonance (EPR) methods are applied for coatings characterization.

Fatty Acid Content and Tumor Growth Changes in Mice After Exposure to Extremely High-Frequency Electromagnetic Radiation and Consumption of N-3 Fatty Acids.

The topical problem is to find new, more effective and safe treatments for cancer. The purpose of the present work was to study the combined effects of low-intensity extremely high-frequency electromagnetic radiation (EHF EMR) and consumption of n-3 polyunsaturated fatty acids (PUFAs) on tumor growth and the content of FAs in the thymus and tumor tissue in mice. Fatty acid composition was determined using gas chromatography.

[Comparative analysis of LAMP and Real Time PCR methods to detect pathogens of glanders and meliodosis.].

Results of detection of Burkholderia mallei and Burkholderia pseudomallei DNA strains by LAMP (Loop-mediated Isothermal Amplification) and Real Time PCR are shown. It has been revealed that, in Real Time PCR, primers steadily detected DNA of those microorganism for the sequences of which they were designed. The above mentioned primers did not detect DNA of heterologous strains.

[The characteristic of diarrheagenic Escherichia separated from children aged under 5 years old in Yaroslavl.].

The diarrheagenic bacteria coli take a significant place among agents of acute intestinal infections in children aged under 5 years. The main danger among these pathogens is represented by both enterotoxigenic E. coli causing enteritis and enterocolitis accompanied by acute dehydration diarrhea and Escherichia producing shiga-toxin being agents of hemorrhagic colitis and hemolytic uremic syndrome.

[Preparation of samples for gas-chromatographic determination of fatty acids: direct transesterification of lipids of dry biological sample is preferred in comparison with the methods employing preliminary lipid extraction.].

Different methods of sample preparation and derivatization were compared from the point of view of product yield, speed and convenience of the technique used. Fatty acid determination in absolutely dry objects (biochemical preparations, food protein isolates, lyophilized microbial biomass) may be performed easily with the use of Folch method provided that 4-component system "chloroform/ methanol/water/acetic acid" was employed. Nevertheless, we ould not find any real advantages of classical Folch or Hara-Radin extraction method variants when compared to simple non-extraction technique (which consists in direct trans-esterification of dried biomaterial due to sequential sample treating with sodium methoxide and boron trifluoride methanolic solutions).

Should the Treatment of Amyloidosis Be Personified? Molecular Mechanism of Amyloid Formation by Aβ Peptide and Its Fragments.

Aβ and Aβ peptides are believed to be associated with Alzheimer's disease. Aggregates (plaques) of Aβ fibrils are found in the brains of humans affected with this disease. The mechanism of formation of Aβ fibrils has not been studied completely, which hinders the development of a correct strategy for therapeutic prevention of this neurodegenerative disorder.

[Molecular mechanism of amyloid formation by Ab peptide: review of own works].

TA characteristic feature of amyloid structures is polymorphism. The study of amyloid structures and their formation process was carried out for synthetic and recombinant Ab(1-40) and Ab(1-42) peptide preparations. In the study of these peptides, we recognized fibrils of different morphologies.

[Study of the transfer of foreign genes into mussel Mytilus Galloprovincialis Lam. eggs by spermatozoa].

The possibility of transferring exogenous DNA into eggs by mussel Mytilus galloprovincialis Lam. sperms both with the use of certain methods of transfection and without them was studied. The efficacy of egg fertilization by sperms treated with foreign DNA and the development of larvae at early stages of embryogenesis were evaluated.

[Plant biotests of soil and water, polluted with petroleum and petroleum products].

Reactiona of higher plants (mustard, oat, rye, salad, dill and barley) and microalgae (Euglena gracilis) on the contamination of soil and water with petroleum and oil products was studied. The germination of seeds was analyzed. The length of sprouts, dry biomass and length of plant roots, as well as the optical density of micro-algal broth culture were determined.

[The transfer of foreign DNA by rabbit spermatozoa into hamster eggs].

Capacitated rabbit sperm was incubated with pCMVlacZ plasmid and then used to fertilize hamster oocytes liberated from zona pellucida. After treatment with DNase I, these oocytes were examined for the presence of exogenous DNA by PCR. DNA of pCMVlacZ was not found in oocytes after simple incubation with male gametes.

[Targeted action proteins as potential drugs].

The data available in the literature over the past decade as to the design and use of directly acting proteins in the treatment of a number of diseases are analyzed. The structure and functions of toxins inhibiting protein synthesis are reviewed. The structural and functional properties of conjugated and recombinant immunotoxins are compared and clinical findings are reviewed.

[Stable expression of heterologous proteins in salmonella: problems and approaches to their designing].

Live attenuated Salmonella vaccines may be used as carriers of heterologous antigens. The optimum expression system for each heterologous antigen requires to be established on an individual basis. This will ensure that the antigen in question is produced at appropriate levels and in the correctly folded conformation.

[Recombinant plasmids carrying yersinia pestis fra-operon: specific features of genetic transmission, inheritance and expression in attenuated enterobacterial cells].

The study was undertaken to study the specific features of transformation of E. coli strains having different R-chemotypes, Y. pestis, S.

[Designing the latex test system for express identification of legionella in the external medium and clinical materials].

A highly sensitive latex test system for identification of Legionella in the external medium and clinical materials have been designed. Protein antigens and polysaccharide components of the outer membrane of the agent were analyzed. Proteins having a molecular mass of 45, 29, and 24 kDa, as well as a polysaccharide component of LPS were found to be common for all L.

[Effect of TRA-system of plasmids RP4 and R68.45 on pseudomonas mallei virulence].

In Pseudomonas mallei, spontaneous mutants (Tra- mutants) of the plasmids RP4 and R68.45, losing the ability to transfer at conjugation are formed. The plasmids RP4 and R68.

[Immunogenic potential of glanders and melioidosis agents].

Unlike the glanders agent, the superficial structures of the melioidosis agent were demonstrated to be responsible for marked was immunosuppressive activity. Some antigenic fractions suppressing the blast transformation of lymphocytes, reducing the count of T helpers and profoundly potentiating the infection in vivo were isolated from P. pseudomallei cells.

[Tularemia vaccine strain is a potential vector].

The immunological and genetic properties of Francisella tularensis vaccine strain are discussion with regard to its use to produce recombinant vaccines. This bacterium-based vector is supposed to be an excellent object for investigating the role of protective antigens in the development of immunity against intracellular bacteria.

[Morphology, ultrastructure and populational features of bacteria francisella].

The morphology, ultrastructure of cells and the structure of microbial populations of various bacteria of the Francisella genus were estimated by electron microscopy. The strain 503 has been found to produce a bacterial population that is most homogeneous in shape and size. It contains microbes of only round and avoid forms, 0.

[Elucidation of functionally active domains in molecules of protective antigen bacillus anthracis's toxin].

Limited proteolysis has established that the protective antigen (PA) molecule consists of four functional-active domains. So, the shielding domain borrows an area in the linear structure of the PA molecule with NH2 of the end up to Lys 166 and plays a conducting role in the proteolytic activation of PA. The associative domain, engaging in the area Arg 167-Met266, plays a key role in the interaction with LF or EF at self-assembly toxic complexes LT or ET.

[Molecular mechanisms underlying bacillus anthracis infection at early stages and search for novel vaccines].

The developmental mechanisms of anthrax immunity were studied. Immunization was found to generally generate specific antibodies and lysozyme. Collectively, all the factors are responsible for suppressing the development of spores in the body.

[Vaccination against typhoid fever: results and perspectives].

The review deals with the world history and the current status of typhoid fever vaccination. It analyzes parenteral and oral killed cellular, oral live attenuated, molecular vaccines are analyzed. The results of field trials of parenteral and oral vaccines in the areas showing different epidemiology and incidence are analyzed.