Semiautomated Production of Cell-Free Biosensors.

Cell-free synthetic biology biosensors have potential as effective diagnostic technologies for the detection of chemical compounds, such as toxins and human health biomarkers. They have several advantages over conventional laboratory-based diagnostic approaches, including the ability to be assembled, freeze-dried, distributed, and then used at the point of need. This makes them an attractive platform for cheap and rapid chemical detection across the globe.

Postoperative adhesions are abrogated by a sustained-release anti-JUN therapeutic in preclinical models.

Postoperative abdominal adhesions are the leading cause of bowel obstruction and a cause of chronic pain and infertility. Adhesion formation occurs after 50 to 90% of abdominal operations and has no proven preventative or treatment strategy. Abdominal adhesions derive primarily from the visceral peritoneum and are composed of polyclonally proliferating tissue-resident fibroblasts.

Ergonomics in the Operating Room: Recommendations for Orthopaedic Surgeons.

Orthopaedic surgeons face notable occupational hazards, including suboptimal ergonomics in the operating room (OR). This results in high rates of musculoskeletal injuries that affect at least four in 10 orthopaedic surgeons. Sex disparities exacerbate these issues, with female surgeons reporting markedly higher rates of occupational injuries and discomfort with surgical instrument use compared with male surgeons.

Connecting Underrepresented Medical Students to Resources and Role Models in Orthopaedic Surgery: A Virtual Diversity, Equity, Inclusion, and Accessibility Summit.

Introduction: Limited access to resources and stereotypes about orthopaedic surgeons may contribute to the low percentage of women and people of underrepresented in medicine (URiM) backgrounds in orthopaedic surgery. Several organizations have created resources to address these barriers, but medical students are unlikely to be exposed to the initiatives through traditional curricula. The purpose of this study was to (1) evaluate the ability of a 1-day virtual Diversity, Equity, Inclusion, and Accessibility (DEIA) summit to effectively reach URiM medical students, (2) increase medical students' knowledge of DEIA resources, and (3) augment the perception of diverse backgrounds in orthopaedic surgery.

Protocol for cell-type-specific single-cell labeling and manipulation in Drosophila using a sparse driver system.

Here, we present a protocol for cell-type-specific single-cell labeling and manipulation in Drosophila using a sparse driver system. We describe steps for generating constructs and fly lines, titrating heat-shocked durations for precise temporal control and desired sparsity, and co-expressing multiple transgenes for experiments. We demonstrate that this generalizable toolkit enables tunable sparsity, multi-color staining, single-cell trans-synaptic tracing, single-cell manipulation, and cell-autonomous gene function analysis.

The relationship between cardiometabolic abnormalities and mortality in the Women's Health Initiative: A comparison of associations among women with cancer to women without cancer.

Background: Prior studies of participants with breast and other obesity-associated cancers in the Women's Health Initiative (WHI) showed worse mortality and cardiovascular disease (CVD) outcomes for individuals with a higher number of cardiometabolic risk factors at study entry. The purpose of this analysis is to compare the relationship between cardiometabolic abnormalities and mortality among women with and without cancer in the WHI.

Methods: Women with one of five early-stage obesity-associated cancers (breast, colorectal, endometrial, ovarian, and non-Hodgkin lymphoma) and controls without any new or prior history of cancer were selected from the WHI-Life and Longevity after Cancer ancillary study.

FFR-Guided Percutaneous Coronary Intervention vs Coronary Artery Bypass Grafting in Patients With Diabetes.

Importance: Outcomes in patients with diabetes after fractional flow reserve (FFR)-guided percutaneous coronary intervention (PCI) using current-generation drug-eluting stents (DES) compared with coronary artery bypass grafting (CABG) are unknown.

Objectives: To investigate the relative treatment effect of PCI vs CABG according to diabetes status with respect to major adverse cardiac and cerebrovascular events (MACCE) at 3 years and to evaluate the impact of the SYNTAX score.

Design, Setting, And Participants: This is a prespecified subgroup analysis of the FAME (Fractional Flow Reserve vs Angiography for Multivessel Evaluation) 3 trial, an investigator-initiated, randomized clinical trial conducted at 48 centers worldwide.

The Case for Preregistering Quasi-Experimental Program and Policy Evaluations.

The recognition that researcher discretion coupled with unconscious biases and motivated reasoning sometimes leads to false findings ("p-hacking") led to the broad embrace of study preregistration and other open-science practices in experimental research. Paradoxically, the preregistration of quasi-experimental studies remains uncommon although such studies involve far more discretionary decisions and are the most prevalent approach to making causal claims in the social sciences. I discuss several forms of recent empirical evidence indicating that questionable research practices contribute to the comparative unreliability of quasi-experimental research and advocate for adopting the preregistration of such studies.

Diagnosis and Treatment of Gestational Diabetes Mellitus: A National Survey of Physician Practices.

We aimed to identify changes in United States practice patterns in gestational diabetes mellitus (GDM) diagnosis and treatment following publication of the 2008 Hyperglycemia and Adverse Pregnancy Outcomes (HAPO) study that supported transition toward a 2-hour oral glucose tolerance test. A total of 1,030 U.S.

Expression of CD47 protein in hematolymphoid neoplasms: Implications for CD47-mediated cancer immunotherapy.

Objectives: Recent studies show that blocking CD47-SIRPα interactions is a promising target in checkpoint inhibition for cancer immunotherapy. However, to date, the expression of CD47 is not well characterized in various hematolymphoid neoplasms.

Methods: This study evaluates CD47 expression in a wide range of hematolymphoid neoplasms using immunohistochemistry on 834 cases.

In-device Battery Failure Analysis.

Lithium-ion batteries are indispensable power sources for a wide range of modern electronic devices. However, battery lifespan remains a critical limitation, directly affecting the sustainability and user experience. Conventional battery failure analysis in controlled lab settings may not capture the complex interactions and environmental factors encountered in real-world, in-device operating conditions.

The Final Rule in a Bind: What We Are Learning From Suboptimal Simultaneous Heart-Kidney Outcomes and Potential Solutions to the Problem.

Background: The 2018 revision of the adult Heart Allocation Policy (aHAP) led to a notable increase in the rate of simultaneous heart-kidney transplants (SHKT) in the United States. However, this policy has faced criticism for its inability to enhance post-transplant survival rates or decrease mortality among SHKT recipients on the waitlist, although high-quality kidneys are used.

Methods: We analyzed data from the Organ Procurement and Transplantation Network, covering 1549 SHKT cases from 2015 to 2021.

"I Thought I Was Going to Die": Identifying Gaps in the Intimate Partner Violence Service Provision System for Black Women.

Black women experience the highest mortality and morbidity resulting from intimate partner violence (IPV) victimization, yet there remains a dearth of culturally responsive interventions designed to meet their needs within the coordinated community response system. We employed the Theory of Help-Seeking Behavior to explicate the barriers that Black women experience when securing assistance from providers within the IPV service provision system, inclusive of the criminal legal, child protective service, shelter, healthcare, and mental healthcare systems. In-depth individual interviews were conducted with 30 people who self-identified as Black women who were help-seeking within the IPV service provision system at the time of data collection.

AAPM-RSS Medical Physics Practice Guideline 9.b: SRS-SBRT.

The purpose of this Medical Physics Practice Guideline (MPPG) is to describe the minimum level of medical physics support deemed prudent for the practice of linear-accelerator, photon-based (linac) stereotactic radiosurgery (SRS), and stereotactic body radiation therapy (SBRT) services. This report is an update of MPPG 9.a published in 2017.

Extent of surgical repair and outcomes after surgery for type A aortic dissection.

Background: Acute Stanford type A aortic dissection is a severe emergency condition that, if left untreated, is associated with a high mortality rate. The extent of surgical repair may impact the outcomes of these patients.

Method: Patients operated for acute type A aortic dissection from a multicentre European registry were included.

A three-stage strategy for conducting an experimental investigation: A recommendation to improve the reproducibility of reported conclusions.

The reproducibility of the results from preclinical research rests on many factors, including the selection of appropriate experimental designs for the individual experiments that constitute the investigation. The design of each of these experiments depends on their purpose within the entire investigation and the information to be gained from conducting them. Here, we explain and justify a three-stage strategy comprising a series of different types of experiment, each with a different purpose and design: a pilot study, a hypothesis-generating experiment and a final hypothesis-confirming experiment.

Histopathologic Features of Pulp Following Uncomplicated Crown Fracture of an Immature Tooth With Concomitant Subluxation Seven Days After the Injury. A Case Report.

Reports on the histopathologic features of pulp tissue following traumatic injuries are scarce. The aim of the present clinical and histological report was to provide some novel insights about the histological condition of the pulp tissue of an immature permanent tooth, shortly after a combined injury of uncomplicated crown fracture with concomitant subluxation. A seven-year-old male patient presented with a localized buccal swelling apically on tooth #21 and with a radiographic app.

Exploring the impact of gender-related variables on health measures and perceived stress.

Introduction: Despite its relevance for health outcomes, only recently gender has begun to be explored in the scientific literature, typically using a rigid binary framework. To tackle this, the Stanford Gender-related Variables for Health Research (GVHR) was developed to analyze gender from a multidimensional perspective. We aim to use our Portuguese version of the GVHR and analyze its association with health outcomes, including perceived stress.

HLA allele and haplotype frequencies of eight Indian populations based on 130,518 registered stem cell donors.

Introduction: In hematopoietic stem cell transplantation, optimal results are achieved when donors and patients are matched regarding their human leukocyte antigen (HLA) genes. Population-specific HLA allele and haplotype frequency distributions determine the probabilities to find matched donors in a stem cell donor registry of given size and ethnic composition.

Methods: To evaluate the needs of Indian patients with regard to future donor recruitment, we analyzed a large data set of =130,518 potential stem cell donors registered with DKMS-BMST, a Bangalore-based donor registry with nationwide donor recruitment activities.

The Role of Routine Plain Film Imaging Post Cochlear Implantation.

Objective: We aimed to evaluate the efficacy of routine post-op X-ray in cochlear implantation patients.

Study Design: Retrospective chart review study.

Setting: Primary or revision cochlear implant patients who had routine postoperative X-ray (XR) or had planned postoperative computed tomography (CT) due to clinical concerns for array malposition.