Support vector regression applied to the determination of the developmental age of a Drosophila embryo from its segmentation gene expression patterns.

Motivation: In this paper we address the problem of the determination of developmental age of an embryo from its segmentation gene expression patterns in Drosophila.

Results: By applying support vector regression we have developed a fast method for automated staging of an embryo on the basis of its gene expression pattern. Support vector regression is a statistical method for creating regression functions of arbitrary type from a set of training data.

Numerical simulation of local heat transfer in rotating two-pass square channels.

3D turbulent air flow and heat transfer developing in a two-pass square channel rotating in the orthogonal mode are simulated using the high-Re k-epsilon turbulence model and a recently developed modification of wall functions. Auxiliary problem for accurate definition of inlet boundary conditions formed by a long unheated upstream section is considered. Details of flow structure are presented.

A method for direct cloning of fur-regulated genes: identification of seven new fur-regulated loci in Escherichia coli.

A strain that allows the cloning of Fur-regulated loci was constructed. The strain, named FUR-SEL1, contains a chromosomal fhuA'-'cat transcriptional fusion that is expressed from the Fur-regulated promoter, fhuA(p). Therefore, Fur boxes introduced on a multicopy plasmid can cause derepression of the fusion by titrating the Fur repressor and thereby confer chloramphenicol resistance, which can be used as a selectable phenotype for cloning Fur-regulated loci.

[Mathematical model of microorganism biomass growth in the absence of a limiting substrate and inhibiting agents].

A mathematical model for the growth of microbial cells upon submerged cultivation was constructed. The model describes the growth phases of the biomass of microorganisms in the absence of inhibiting agents and limitation by substrate in the medium. At the basis of model is the requirement that cells should not be in physical contact during some time in order that they can divide.

Hydrodynamics and mass transfer of the coaxial jet mixer in flow injection analysis.

A coaxial jet mixer that was previously proposed for rapid and efficient mixing under laminar flow conditions has been studied both theoretically and experimentally. A mathematical model that consists of a set of Navie-Stokes equations that determine the flow velocities and three diffusion-convection reaction equations that determine the reactant and product concentrations has been developed. Equations are solved with the help of finite difference techniques for different flow conditions.

Queueing models of potentially lethal damage repair in irradiated cells.

Some of the ideas arising in queueing theory are applied to describe the repair mechanisms responsible for recovery of cells from potentially lethal radiation damage. Two alternative versions are presented of a queueing model of damage repair after a single dose of irradiation. The first version represents a linear misrepair model, and the second invokes the idea of spontaneous lesion fixation.