Testing for thermal acclimation in zoospores of an amphibian pathogen.

Thermal acclimation effects on locomotory performance have been widely documented for macroscopic organisms, but such responses remain largely unexplored in microorganisms. Metabolic theory of ecology (MTE) predicts faster responses in smaller organisms, with potential consequences for host-parasite interactions in variable temperature environments. We investigated thermal acclimation effects on zoospores of the amphibian fungal pathogen Batrachochytrium dendrobatidis (Bd), quantifying (1) thermal performance for maximum zoospore velocity and (2) high temperatures needed to immobilize 50% (CT50max) or 100% (CT100max) of zoospores.

Landscape-scale drivers of spatial dynamics and genetic diversity in an emerging wildlife pathogen.

Aquatic pathogens often cannot tolerate drying, and thus their spread, and diversity across a landscape may depend on interactions between hydrological conditions and the movement of infected hosts. The aquatic fungus Batrachochytrium dendrobatidis (Bd) is a nearly ubiquitous pathogen of amphibians and particular lineages have been associated with host declines. By coupling amphibian surveys with molecular pathogen detection and genotyping techniques, we characterized the spatial dynamics and genetic diversity of Bd on a landscape containing both permanent and ephemeral ponds.

Trigonal bipyramidal or square planar? Density functional theory calculations of iron bis(dithiolene) N-heterocyclic carbene complexes.

Density functional theory (DFT) calculations of 57 iron bis(dithiolene)-N-heterocyclic carbene adducts were conducted to determine what parameters predict, and possibly influence, the coordination of these aforementioned adducts. The parameters considered herein include three different types of nuclear magnetic resonance (C-NMR, Se-NMR, and P-NMR) isotropic chemical shifts, the Tolman Electronic Parameter (TEP), the Huynh Electronic Parameter (HEP), and the percent buried volume (%) of the different N-heterocyclic carbenes (NHCs) calculated from DFT. These parameters were selected based upon prior literature connection to σ-donor ability, π-acidity, and steric effects.

Prediction of adverse drug reactions using demographic and non-clinical drug characteristics in FAERS data.

The presence of adverse drug reactions (ADRs) is an ongoing public health concern. While traditional methods to discover ADRs are very costly and limited, it is prudent to predict ADRs through non-invasive methods such as machine learning based on existing data. Although various studies exist regarding ADR prediction using non-clinical data, a process that leverages both demographic and non-clinical data for ADR prediction is missing.

The pregnancy rescue case versus typical abortion.

Hendricks' pregnancy rescue case (PRC) tries to show that abortion is typically morally wrong. I argue here that there are at least two morally relevant differences between the abortion in PRC and the typical abortion so that the latter isn't morally wrong even if the former is morally wrong. I develop five modifications to PRC to show that these two differences are morally important.

Building Chemical Intuition about Physicochemical Properties of C8-Per-/Polyfluoroalkyl Carboxylic Acids through Computational Means.

We have predicted acid dissociation constants (p ), octanol-water partition coefficients ( ), and DMPC lipid membrane-water partition coefficients ( ) of 150 different eight-carbon-containing poly-/perfluoroalkyl carboxylic acids (C8-PFCAs) utilizing the COnductor-like Screening MOdel for Realistic Solvents (COSMO-RS) theory. Different trends associated with functionalization, degree of fluorination, degree of saturation, degree of chlorination, and branching are discussed on the basis of the predicted values for the partition coefficients. In general, functionalization closest to the carboxylic headgroup had the greatest impact on the value of the predicted physicochemical properties.

Broken chromosomes heading into mitosis: More than one way to patch a flat tire.

A cell dealing with a broken chromosome in mitosis is like a driver dealing with a flat tire on the highway: damage repair must occur under non-ideal circumstances. Mitotic chromosome breaks encounter problems related to structures called micronuclei. These aberrant nuclei are linked to cell death, mutagenesis, and cancer.

Mechanical crosstalk between the intervertebral disc, facet joints, and vertebral endplate following acute disc injury in a rabbit model.

Background: Vertebral endplate sclerosis and facet osteoarthritis have been documented in animals and humans. However, it is unclear how these adjacent pathologies engage in crosstalk with the intervertebral disc. This study sought to elucidate this crosstalk by assessing each compartment individually in response to acute disc injury.

Graham Hoyle (1923-1985): exploring the depths of muscle diversity.

Graham Hoyle was an important neuroscientist, muscle biologist, and zoologist throughout much of the second half of the twentieth century. A native of England, Hoyle studied under Bernard Katz in London before earning his D.Sc.

Digital economy and inclusive green growth: The moderating effect of government environmental regulation.

As digital economy flourishes, there have been fundamental changes in the patterns of economic development, social structure, and ecological environment, which are the key elements of inclusive green growth (IGG). However, whether digital economy can effectively contribute to IGG requires further investigation. This paper evaluates the direct and spatial spillover effect of digital economy on IGG of 280 cities in China during 2011-2019 using Spatial Durbin Model (SDM).

Abortion, Impairment, and Well-Being.

Hendricks' The Impairment Argument (TIA) claims that it is immoral to impair a fetus by causing it to have fetal alcohol syndrome (FAS). Since aborting a fetus impairs it to a greater degree than causing it to have FAS, then abortion is also immoral. In this article, I argue that TIA ought to be rejected.

A Tunable Calcium Phosphate Coating to Drive in vivo Osseointegration of Composite Engineered Tissues.

Varying degrees of hydroxyapatite (HA) surface functionalization have been implicated as the primary driver of differential osteogenesis observed in infiltrating cells. The ability to reliably create spatially controlled areas of mineralization in composite engineered tissues is of growing interest in the field, and the use of HA-functionalized biomaterials may provide a robust solution to this challenge. In this study, we successfully fabricated polycaprolactone salt-leached scaffolds with two levels of a biomimetic calcium phosphate coating to examine their effects on MSC osteogenesis.

MAdCAM-1 costimulation in the presence of retinoic acid and TGF-β promotes HIV infection and differentiation of CD4+ T cells into CCR5+ TRM-like cells.

CD4+ tissue resident memory T cells (TRMs) are implicated in the formation of persistent HIV reservoirs that are established during the very early stages of infection. The tissue-specific factors that direct T cells to establish tissue residency are not well defined, nor are the factors that establish viral latency. We report that costimulation via MAdCAM-1 and retinoic acid (RA), two constituents of gut tissues, together with TGF-β, promote the differentiation of CD4+ T cells into a distinct subset α4β7+CD69+CD103+ TRM-like cells.

The impairment argument, ethics of abortion, and nature of impairing to the n + 1 degree.

I argue here that the impairment principle requires clarification. It needs to explain what makes one impairment greater than another, otherwise we will be unable to make the comparisons it requires, the ones that enable us to determine whether b really is a greater impairment than a, and as a result, whether causing b is immoral because causing a is. I then develop two of what I think are the most natural accounts of what might make one impairment greater than another.

The indirect effects of food insecurity on obesogenic environments.

Introduction: The Centers for Disease Control and Prevention (CDC) estimates 39.8% of United States (US) residents have obesity. This study examined obesity-related factors at the county-level to determine the indirect effects on physical inactivity, insufficient sleep duration, income inequality, food insecurity, on obesity rates.

The flowering time regulator FLK controls pathogen defense in Arabidopsis thaliana.

Plant disease resistance is a complex process that is maintained in an intricate balance with development. Increasing evidence indicates the importance of posttranscriptional regulation of plant defense by RNA binding proteins. In a genetic screen for suppressors of Arabidopsis (Arabidopsis thaliana) accelerated cell death 6-1 (acd6-1), a small constitutive defense mutant whose defense level is grossly in a reverse proportion to plant size, we identified an allele of the canonical flowering regulatory gene FLOWERING LOCUS K HOMOLOGY DOMAIN (FLK) encoding a putative protein with triple K homology (KH) repeats.

Evidence-Based Classroom Observation Technique: An Interdisciplinary, Structured Approach to Classroom Observation.

Classroom observation is commonplace in higher education, but the process itself varies from school to school. A literature search revealed a lack of structured tools and universal interdisciplinary processes for postsecondary classroom observation. Thus, the Evidence-Based Classroom Observation Technique was developed to include both the tool and the interdisciplinary process.

Measuring the State Dependence Effect in Hospital Payment Adjustment.

Since FY 2013, as a part of the Affordable Care Act (ACA) program, the Hospital Value-Based Purchasing (HVBP) program has adjusted Medicare's payments to hospitals based on the total performance score of the hospital. First, the program reduces a portion of the hospital's Medicare payments in a specific fiscal year, and then, by the end of the same fiscal year, the amount of the payment reductions will be awarded to the hospitals based on the total performance score; thus, the hospitals that do not receive the reward will lose the portion of money reduced by Medicare. In this research, we apply the theory of state dependence and use the dynamic random effect probit model to estimate this effect.

Omadacycline for a Carbapenem-Resistant -Associated Wound Infection.

Carbapenem-resistant Enterobacteriaceae (CRE) are problematic pathogens because infections caused by these organisms are associated with significant morbidity and mortality. These organisms often harbor multiple resistance mechanisms, which makes it difficult to treat their associated infections. Treatment typically consists of intravenous antibiotics that are selected based on the specific susceptibility pattern for the pathogen.

Efficient and trusted autonomous vehicle routing protocol for 6G networks with computational intelligence.

The Internet of Things (IoT) and wireless sensors have collaborated with many real-time environments for the collection and processing of physical data. Mobile networks with sixth-generation (6G) technologies provide support for emerging applications using Connected and Autonomous Vehicles (CAV) and observe critical conditions. Although, autonomous vehicle-based routing solutions have presented significant development toward reliable and inter-vehicle communications.

A Q Methodology Investigation of School Counselors' Beliefs and Feelings in Reporting Suspected Child Sexual Abuse.

School counselors can be influenced by a wide variety of beliefs and emotions when reporting suspected child sexual abuse (CSA) including worry, fear, sadness, depression, helplessness, anger, and distrust of child protective services. These beliefs and emotions can keep school counselors from reporting suspected CSA despite their duties as mandated reporters. We sought to uncover patterns of school counselors' ( = 85) shared beliefs and emotions that can impact their decision making in reporting suspected CSA.