Repair of Cartilage Defects Using ATDC5 Cells Treated with BBR Loaded in Chitosan Hydrogel.

In this study, we explore the cartilage defect repair mechanism by phosphocreatine-grafted chitosan hydrogels loaded with berberine-treated ATDC5 cells (CSMP@BBR@ATDC5). Under the optimal concentrations of LPS and BBR ideal conditions, ATDC5 cell toxicity and proliferation were detected with AM/PI and EdU staining. Additionally, qPCR and Western blot were employed to detect the expression of the SIRT1/BMP4 signaling pathway and chondrogenic-related factors in ATDC5 cells.

Knee proprioception four months after anterior cruciate ligament reconstruction: Impact of limb dominance, anterolateral procedure, and association with readiness to return to sport.

Background: Knee proprioception may be compromised after anterior cruciate ligament reconstruction (ACLR), but associated factors and impact remain unclear. This study evaluated knee proprioception 4 months after primary ACLR, compared with healthy controls, and explored the impacts of leg dominance, anterolateral procedures (AEAPs), and their association with psychological readiness to return to sports.

Methods: This prospective cohort study included 30 ACLR participants and 20 healthy controls.

Feasibility, reliability, and validity of the two-minute step test for assessing aerobic exercise capacity and functional endurance in participants with anterior cruciate ligament reconstruction.

The aim of this study was to investigate the feasibility, test-retest reliability, and construct validity of the 2-min step test (2MST) in measuring aerobic exercise capacity and functional endurance in participants with anterior cruciate ligament reconstruction (ACLR). Fifty participants with quadriceps tendon autograft, bone-patellar tendon-bone graft, or hamstring autograft were included. Feasibility was assessed by the time and support required to complete the measures and the feedback from participants, including their satisfaction ratings.

Functional knee bracing provides protection to the anterior cruciate ligament in response to rotatory loads.

Background: The aim of the study was to quantify the effect of functional knee bracing on native knee kinematics and the in-situ force in the ACL in response to external loading.

Methods: A robotic testing system was used to apply three external loads from full extension to 60° of flexion to eight fresh frozen human cadaveric knees: 1) a 134 N anterior load, 2) a combined 5-Nm internal rotation +5-Nm valgus torque, and 3) a combined 5-Nm external rotation +5-Nm valgus torque. For native and braced states, kinematics were recorded and the in-situ force in the ACL was determined.

Impact of COVID-19 lock-down period on orthopedic and trauma surgical activity in a northern Italian hospital.

Objectives: This study aims to describe the impact of COVID-19 on Orthopedic and Trauma surgical activity in a single level-I trauma center in Northern Italy during the lockdown period. We proposed comparing surgical procedures performed during the outbreak and in the same period the previous year.

Methods: In this single-center retrospective epidemiological cohort study, the "lockdown cohort" of patients who were treated from March 1 to May 24, 2020, was compared to the "control cohort" who received treatment during the same period in 2019.

Modified Distalization Shoulder Angle and Lateralization Shoulder Angle show weakly correlation with clinical outcomes following reverse shoulder arthroplasty.

Introduction: The focus in reverse shoulder arthroplasty (RSA) has been on the lateralization and distalization of prosthesis positioning, influenced by implant design and surgical technique. There's no consensus on the optimal amount of lateralization and distalization or the best radiographic parameters for evaluating placement. This study examines the correlation and the predictive value between previously described modified distalization shoulder angle (DSA) and lateralization shoulder angle (LSA) with postoperative outcomes, which aim to differentiate the contributions of the humeral and glenoid components in the global distalization and lateralization of the RSA.

Can transcranial direct current stimulation (tDCS) over the motor cortex increase endurance running performance? a randomized crossover-controlled trial.

Transcranial direct current stimulation (tDCS), a non-invasive brain stimulation technique, has been shown to increase exercise performance in strength and cycling studies but its effects on running endurance remain unclear. The objectives of this randomized sham-controlled crossover trial were to assess tDCS efficacy on submaximal treadmill running time to exhaustion (TTE). Forty-five healthy male runners aged between 18 and 32 years (mean maximal oxygen consumption: 46.

The effectiveness of microfracture augmentation on the wall of femoral condyle in meniscal healing among patients with meniscus tears after sports injury.

Background: Meniscus injuries are familiar sources of knee pain, with meniscus repair sometimes yielding unsatisfactory results. Microfracture is a standard procedure for treating articular cartilage damage in the knee that promotes the formation of fibrocartilage over damaged cartilage. Microfracture enhanced the healing rate of meniscus repair in animal models.

Predictive analysis of economic and clinical outcomes in total knee arthroplasty: Identifying high-risk patients for increased costs and length of stay.

Purpose: The purpose of this study was to predict high-risk patients who experience significant increases in hospital charges and length of stay (LOS) following specific postoperative complications.

Methods: This study analyzed over two million patients from the Nationwide Inpatient Sample database undergoing elective total knee arthroplasty (TKA) for primary osteoarthritis. Baseline demographics, clinical characteristics and incidence of postoperative complications were examined.

Inappropriate grouping of various kinematic alignment techniques and inconsistent reporting of adverse events invalidate comparison across studies: An umbrella review of meta-analyses.

Purpose: This umbrella review aimed to identify, synthesise and critically appraise the findings of meta-analyses that compare adverse events-rates of complications, reoperations and revisions-following total knee arthroplasty (TKA) using unrestricted kinematic alignment versus mechanical alignment.

Methods: Using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses criteria, two authors independently screened articles based on inclusion and exclusion criteria, and assessed the methodological quality based on the 16 domains of A MeaSurement Tool to Assess systematic Reviews (AMSTAR-2). Effect sizes of difference in rates of complications were tabulated for each meta-analysis.

Effects of different swimming styles on postural assessment in mid-level young swimmers.

It is well-known that swimming purposes to increase the tonic-postural control. Beyond its physiological advantages, swimming also offers an exclusive platform to explore the complex interplay between body biomechanics and posture. The specific aim of this study was to investigate the effects of main swimming styles on postural balance in young athletes.

Matrix-assisted autologous chondrocyte transplantation is effective at mid/long-term for knee lesions: A systematic review and meta-analysis.

Purpose: This systematic review with meta-analysis evaluates the long-term efficacy of matrix-assisted autologous chondrocyte transplantation (MACT) in terms of functional scores using scaffolds made of hyaluronic acid (HA) or collagen (C).

Methods: Nineteen articles met the eligibility criteria for the analysis. Fourteen studies focused on patients treated with MACT with HA-based scaffolds, four studies with C-based scaffolds, and one study compared both scaffold types.

Biological strategies in rotator cuff repair: a clinical application and molecular background.

Conventional repair of rotator cuff tears bears a variable but significant risk of incomplete healing. Biological therapies that accompany surgical rotator cuff repair include platelet-rich plasma, stem cells of different origins, and biological scaffolds. Biological therapies facilitate the regeneration of the correct microarchitecture of the tendon attachment to the bone and reduce failures after surgical rotator cuff repair.

Large language models' performances regarding common patient questions about osteoarthritis: A comparative analysis of ChatGPT-3.5, ChatGPT-4.0, and perplexity.

Background: Large Language Models (LLMs) have gained much attention and, in part, have replaced common search engines as a popular channel for obtaining information due to their contextually relevant responses. Osteoarthritis (OA) is a common topic in skeletal muscle disorders, and patients often seek information about it online. Our study evaluated the ability of 3 LLMs (ChatGPT-3.

Learning curve for combined reconstruction of the anterolateral and anterior cruciate ligaments: a report of 108 cases with a single surgeon.

Background: Anterior cruciate ligament (ACL) rupture is a common lesion among young sports players, for whom isolated surgical repair remains the gold standard. However, there is a high risk of re-rupture after ACL reconstruction. Recent results of ACL reconstruction combined with reconstruction of the anterolateral ligament (ALL) have shown a significant decrease in this risk.

Is pre-operative block-test with lidocaine injection efficient in predicting the functional result of revision total hip arthroplasty?

Background: While performing a hip joint aspiration for culture, a lidocaine diagnostic injection called block test can be performed during the investigation of painful total hip arthroplasties (THA). This test was formerly applied to limited series in pre-operative and without assessing the predictive value on the results of THA revision. Therefore we investigated a consecutive series of THA revisions who underwent pre-operative aspiration-block test to determine if patients with pre-operative positive block test (disappearance of symptoms) have a better clinical improvement after revision.

Translation, cross-cultural adaptation, and clinimetric properties evaluation of the Danish version of the Anterior Cruciate Ligament - Return to Sport after Injury scale (ACL-RSI).

Purpose: To translate and cross-cultural adapt the Anterior Cruciate Ligament-Return To Sport After Injury (ACL-RSI) questionnaire into Danish (ACL-RSI-DK) and evaluate the psychometric properties with the purpose to identify the psychological readiness on returning to sport (RTS).

Methods: The ACL-RSI-DK followed a six-step translation and cultural-adaptation process. Statistical analysis included correlation for construct validity of the ACL-R-DK with the Lysholm, KOOS and K-SES.

Effect of Platelet-Rich Plasma Injection on the Treatment of Achilles Tendinopathy: A Systematic Review and Meta-analysis.

Background: Achilles tendinopathy is a common condition without a reproducible and timely treatment modality. Platelet-rich plasma (PRP) injection has been proposed as an enticing treatment option, but there is no consensus regarding its effectiveness.

Purpose: To pool the available data and evaluate the evidence of the effect of PRP injections on Achilles tendinopathy.