Evolutionary Pro-To-Thr Mutation in the Intrinsically Disordered Domain of ANP32 Family Members Modulates Their Target Binding Modes.

Gene duplication has allowed protein evolution toward novel functions and mechanisms. The differences between paralogous genes frequently rely on the sequence of disordered regions. For instance, in mammals, the chaperones ANP32A and ANP32B share a common evolutionary line and have some exchangeable functions based on their similar N-terminal domains.

Inter-Laboratory Validation of Nodal/Paranodal Antibody Testing.

Background And Aims: Reliable detection of antibodies against nodal targets is vital for the diagnosis of autoimmune nodopathies. The performance characteristics of recently developed in-house assays are unknown. We compared testing at four centres.

Evaluation of Semiempirical Quantum Mechanical Methods for Zr-Based Metal-Organic Framework Catalysts.

Zr-based metal-organic frameworks (MOFs) are typically employed in heterogeneous catalysis due to their porosity, chemical and thermal stability, and well-defined active sites. Density functional theory (DFT) is the workhorse to compute their electronic structure; however, it becomes very costly when dealing with reaction mechanisms involving large unit cells and vast configurational spaces. Semiempirical quantum mechanical (SQM) methods appear as an alternative approach to simulate such chemical systems at low computational cost, but their feasibility to model catalysis with MOFs is still unexplored.

International multistakeholder consensus statement on post-publication integrity issues in randomized clinical trials by Cairo Consensus Group.

The number of retractions of randomized clinical trials (RCTs) following post-publication allegations of misconduct is increasing. To address this issue, we aimed to establish an international multistakeholder consensus on post-publication integrity concerns related to RCTs. After prospective registration (https://osf.

Identification of the Novel HLA-A*01:478 Allele by Next-Generation Sequencing.

HLA-A*01:478, a novel HLA class I allele detected by next-generation sequencing.

Characterisation of the Novel HLA-DQB1*03:571 Allele by Next-Generation Sequencing.

HLA-DQB1*03:571 differs from HLA-DQB1*03:09 by one nucleotide substitution in codon 170 in exon 3.

A Long-Term Ecological Research Data Set From the Marine Genetic Monitoring Program ARMS-MBON 2018-2020.

Molecular methods such as DNA/eDNA metabarcoding have emerged as useful tools to document the biodiversity of complex communities over large spatio-temporal scales. We established an international Marine Biodiversity Observation Network (ARMS-MBON) combining standardised sampling using autonomous reef monitoring structures (ARMS) with metabarcoding for genetic monitoring of marine hard-bottom benthic communities. Here, we present the data of our first sampling campaign comprising 56 ARMS units deployed in 2018-2019 and retrieved in 2018-2020 across 15 observatories along the coasts of Europe and adjacent regions.

Sustainable Electrospun Hybrid Nanofibers for Triboelectric Nanogenerators.

Triboelectric nanogenerators (TENGs) have emerged as potential energy-harvesting modules for miniaturized devices. TENG modules are derived often from components having low sustainability whereas the current environmental and economic circumstances demand a focus on sustainable, ecologically friendly approaches for the development of advanced hybrid materials. Herein, recycled polyethylene terephthalate (PET) along with commercially available nylon are electrospun into nanofibers for TENG devices.

Oral antibiotic prophylaxis induces changes in the microbiology of surgical site infection after colorectal surgery. A matched comparative study.

Aim: Oral antibiotic prophylaxis (OAP) lowers rates of surgical site infection (SSI) and may aid anastomotic healing in colorectal surgery. The aim of this study was to analyse the understudied impact of OAP on SSI microbiology after colorectal surgery.

Method: A post hoc analysis was performed on a previous prospective, multicentre study of elective colorectal surgery.

Effect of Semen Collection in the Metabolite and Hormonal Content of Rabbit Seminal Plasma.

Blood serum (BS) and seminal plasma (SP) share a plethora of compounds that might present an individual and/or temporal concentration variation. We aimed to determine whether BS and SP concentrations of albumin, calcium, citrate, creatinine, fructose, glucose, lactate, total protein, urea, zinc, cortisol, anti-Müllerian hormone (AMH) and testosterone are related to weekly collections in New Zealand White (NZW) adult rabbit bucks. During a 12-week study, blood samples were obtained at the beginning and the end of the study period, and semen samples were taken twice a week from four NZW adult rabbit bucks, starting at 6-7 months of age.

Unlocking the Link Between Gut Microbiota and Psychopathological Insights in Anorexia Nervosa: A Systematic Review.

Objective: This systematic review explores the associations between qualitative/quantitative changes in gut microbiota and psychopathological symptoms or other clinical features in patients with eating disorders (EDs). Secondary outcomes include exploring gut microbiota changes in EDs and potential relationships with psychotropic drug use.

Method: A systematic search was conducted across biomedical databases from inception to June 2024 according to PRISMA guidelines.

Taxonomic and phylogenetic biases in translocated angiosperm plant species across European countries.

Conservation translocations are a well-known conservation tool used to reverse the effects of local population extinctions and restore ecosystems. Compared with mammals and birds, plants are underrepresented in translocation programs, and little is known about the potential taxonomic and phylogenetic biases of plant translocation efforts. We aimed to assess how translocated plant species may contribute to the conservation of phylogenetic diversity (PD) among European countries.

Toward a Comprehensive Understanding of Nurses' Compassion Fatigue: A Meta-Integrative Qualitative Synthesis.

Aims: The study aims to conduct a thematic synthesis approach of existing qualitative research to synthesise the real experiences of nurses dealing with compassion fatigue. This integration seeks to provide a comprehensive overview, identify common themes, and offer theoretical support for developing effective intervention strategies to mitigate compassion fatigue in healthcare settings.

Design: A thematic synthesis approach was utilised, synthesising qualitative studies on nurses' experiences of compassion fatigue.

Normothermic Machine Perfusion vs. Static Cold Storage in Liver Transplantation: A Systematic Review and Meta-Analysis.

Background: Normothermic machine perfusion (NMP) represents an alternative to prolong liver preservation and reduce organ discard rates. We performed an updated systematic review and meta-analysis to compare NMP with static cold storage (SCS) in liver transplantation.

Methods: MEDLINE, Embase, and Cochrane were searched for randomized controlled trials (RCTs) or observational studies.

Tolerance to NSAIDs in Actinobacteria From a Mexican Volcano Crater: Genomics and Bioremediation Potential.

Non-steroidal anti-inflammatory drugs (NSAIDs) are emerging contaminants that pose significant health and environmental risks due to their persistence, including their presence in drinking water. Bioremediation, particularly through microorganisms such as actinobacteria, offers a sustainable approach to mitigate these pollutants. Actinobacteria from poly-extreme environments exhibit unique genetic and metabolic adaptations, enabling resistance to and degradation of various contaminants.

Exploring the Impact of Non-Ablative Erbium Laser Therapy on Recurrent Vaginal Candidiasis.

Objectives: To assess the effect of non-ablative Er:YAG laser therapy on the symptoms and microbiological conditions of women with recurrent vulvovaginal candidiasis (RVVC).

Materials And Methods: One hundred and sixty-seven women (mean age 34.1 ± 8.

Approaches for GC-HRMS Screening of Organic Microcontaminants: GC-APCI-IMS-QTOF versus GC-EI-QOrbitrap.

This study explores the capabilities of GC-APCI-IMS-QTOF MS and GC-EI-QOrbitrap MS in screening applications and different strategies for wide-scope screening of organic microcontaminants using target suspect and nontarget approaches. On one side, GC-APCI-IMS-QTOF MS excels at preserving molecular information and adds ion mobility separation, facilitating screening through the list of componentized features containing accurate mass, retention time, CCS, and fragmentation data. On the other side, the extensive and robust fragmentation of GC-EI-QOrbitrap MS allows the application of different strategies for target and nontarget approaches using the NIST library spectra.

Lessons learned from the COVID-19 pandemic on the global health needs of young children: A cross-sectional study.

Background: This cross-sectional study assessed the global health needs of children aged 2 to 6 years and examined how socio-demographic characteristics influenced children's health needs observed following the COVID-19 pandemic.

Methodology: Cross-sectional study conducted between January and March 2021 in three regions of northern Spain with similar household incomes. Participants were selected through one-stage cluster sampling.

Body Shapes of Multiple Anthropometric Traits and All-cause and Cause-specific Mortality in the UK Biobank.

Background: Individual traditional anthropometric measures such as body mass index and waist circumference may not fully capture the relation of adiposity to mortality. Investigating multitrait body shapes could overcome this limitation, deepening insights into adiposity and mortality.

Methods: Using UK Biobank data from 462,301 adults (40-69 years at baseline: 2006-2010), we derived four body shapes from principal component analysis on body mass index, height, weight, waist and hip circumference, and waist-to-hip ratio.

DP4+-Based Stereochemical Reassignment and Total Synthesis of Polyenic Macrolactam Muanlactam.

The total synthesis of the stereoisomer of muanlactam predicted by DP4+ calculations, which differed from that reported for the natural product on the relative configuration at C19, was completed, and the structure of the polyenic macrolactam was fully confirmed. Construction of the stereocenters involved the iterative enantio- and diastereoselective Krische's allylation reaction for the formal -1,3-diol and the addition of a propargylic Grignard reagent to Ellman's chiral nonracemic -butylsulfinamide for the enantiopure amine fragment. The conjugated triene and diene units were constructed by Suzuki-Miyaura cross-coupling reactions of the corresponding alkenylboronates and alkenyl iodides.

Janus kinase inhibitors in the management of acute severe ulcerative colitis: a comprehensive review.

Background: One third of patients with acute severe ulcerative colitis (ASUC) are steroid-refractory. Cyclosporine and infliximab are currently the mainstays of salvage therapy. Janus kinase inhibitors (JAKi) could play a role in the treatment of ASUC.

Outcome measures in muscular dystrophy rehabilitation: an ICF content comparison approach to the most commonly used MD scales.

Introduction: Functioning is the reason to be of rehabilitation as it is essential to the lives of people who suffer from a disease. The International Classification of Functioning, Disability and Health (ICF) can help in designing a functioning profile of a patient, identifying needs for rehabilitation plans and measuring the results of an intervention.

Objective: To identify the outcome measurement instruments reported in clinical studies in muscular dystrophies (MDs) and provide an ICF content analysis.