Effects of Long-Term Fasting on Gut Microbiota, Serum Metabolome, and Their Association in Male Adults.

Background: Long-term fasting demonstrates greater therapeutic potential and broader application prospects in extreme environments than intermittent fasting.

Method: This pilot study of 10-day complete fasting (CF), with a small sample size of 13 volunteers, aimed to investigate the time-series impacts on gut microbiome, serum metabolome, and their interrelationships with biochemical indices.

Results: The results show CF significantly affected gut microbiota diversity, composition, and interspecies interactions, characterized by an expansion of the Proteobacteria phylum (about six-fold) and a decrease in Bacteroidetes (about 50%) and Firmicutes (about 34%) populations.

Bayesian source apportionment and eco-probabilistic risk assessment of aliphatic and polycyclic aromatic hydrocarbons in sediments from the offshore area between the Deep Water Tano and West Cape three points blocks, Ghana.

This study investigates aliphatic and polycyclic aromatic hydrocarbons in sediments from offshore Ghana, focusing on their distribution, sources, and ecological risk. Samples were collected from 15 sites near Deep Water Tano and West Cape Three Points blocks. GC-FID and GC-MS analyses revealed higher concentrations in West Cape Three Points compared to Deep Water Tano.

Multiplex influences on vigilance and biochemical variables induced by sleep deprivation.

Introduction: Sleep loss and sleep deprivation (SD) cause deleterious influences on health, cognition, mood and behaviour. Nevertheless, insufficient sleep and SD are prevalent across many industries and occur in various emergencies. The deleterious consequences of SD have yet to be fully elucidated.

Effect of 15 days -6° head-down bed rest on microbial communities of supragingival plaque in young men.

Introduction: The microgravity environment astronauts experience during spaceflight can lead to an increased risk of oral diseases and possible changes in oral microecology. In this study, we aimed to assess changes in the microbial community of supragingival plaques to explore the effects of spaceflight microgravity environment on oral microecology.

Methods: Sixteen healthy male volunteers were recruited, and supragingival plaque samples were collected under -6° head-down bed rest (HDBR) at five-time points: day 1 before HDBR; days 5, 10, and 15 of HDBR; and day 6 of recovery.

Developing an efficient climate forecasting model for the spatiotemporal climate dynamics estimation and the prediction that fits the variable topography feature of the upper Blue Nile basin, Ethiopia.

The spatiotemporal climate estimation and prediction are challenging and demanding for variable topography feature areas. Spatially, the upper Blue Nile basin (UBNB) is unsatisfactory owing to complex topographical features and the lack of accurate climate prediction and estimation models. Yet accurate information and reliable seasonal climate dynamics estimation and forecasting are essential in the region for controlling reservoir operation and flooding prevention.

Optimal selection of daily satellite precipitation product based on structural similarity index at 1 km resolution for the Pra catchment, Ghana.

Thirteen satellite precipitation products (SPPs), re-gridded to 1 km resolution, were evaluated in terms of the structural similarity index (SSI) over the Pra catchment in Ghana. Three SPP scenarios were considered: Scenario one (S1) was the original SPPs; Scenario two (S2) was bias-corrected SPPs; and Scenario three (S3) was the better of S1 and S2 for each wet day. For each scenario, the best SPP was selected to constitute the 14th SPP referred to as the BEST SPP.

Advances in the Synthesis of Covalent Triazine Frameworks.

Covalent triazine frameworks (CTFs) are a class of organic polymer materials constructed by aromatic 1,3,5-triazine rings with planar π-conjugation properties. CTFs are highly stable and porous with N atoms in the frameworks, possessing semiconductive properties; thus they are widely used in gas adsorption and separation as well as catalysis. The properties of CTFs strongly depend on the type of monomers and the synthesis process.

Rumba Dance Combined with Breathing Training as an Exercise Intervention in the Management of Stress Urinary Incontinence in Postmenopausal Women: A Randomized Controlled Trial.

Purpose: Stress urinary incontinence (SUI) refers to involuntary leakage from the urethra, synchronous with exertion/effort, sneezing or coughing, which has a negative effect on quality of life. Studies have shown that mild-to-moderate physical activities reduce the risk of SUI by multiple mechanisms. The objective of this study was to determine whether the Rumba dance combined with breathing training (RDBT) can reduce the severity of incontinence and improve the quality of life of patients with SUI.

Short-term head-down bed rest microgravity simulation alters salivary microbiome in young healthy men.

Microgravity influences are prevalent during orbital flight and can adversely affect astronaut physiology. Notably, it may affect the physicochemical properties of saliva and the salivary microbial community. Therefore, this study simulates microgravity in space using a ground-based -6° head-down bed rest (HDBR) test to observe the effects of microgravity on oral salivary secretion function and the salivary microbiome.

Hepatic metabolite responses to 4-day complete fasting and subsequent refeeding in rats.

Background: Fasting has been widely used to improve various metabolic diseases in humans. Adaptive fasting is necessary for metabolic adaptation during prolonged fasting, which could overcome the great advantages of short-term fasting. The liver is the main organ responsible for energy metabolism and metabolic homeostasis.

Effects of 10-Day Complete Fasting on Physiological Homeostasis, Nutrition and Health Markers in Male Adults.

Fasting shows great potential in preventing chronic diseases and has to be surmounted under some extraordinary circumstances. This study aimed to investigate the safety, time effects of metabolic homeostasis and health indexes during prolonged fasting. Thirteen participants were recruited to conduct a 10-day complete fasting (CF) in a controlled health research building under medical supervision including 3-day Baseline (BL), 10-day CF, 4-day calorie restriction (CR) and 5-day full recovery (FR).

Non-Invasive Skin Imaging Assessment of Human Stress During Head-Down Bed Rest Using a Portable Handheld Two-Photon Microscope.

Spaceflight presents a series of physiological and pathological challenges to astronauts resulting from ionizing radiation, microgravity, isolation, and other spaceflight hazards. These risks cause a series of aging-related diseases associated with increased oxidative stress and mitochondria dysfunction. The skin contains many autofluorescent substances, such as nicotinamide adenine dinucleotide phosphate (NAD(P)H), keratin, melanin, elastin, and collagen, which reflect physiological and pathological changes .

Effects of 15-Days -6° Head-Down Bed Rest on the Attention Bias of Threatening Stimulus.

Previous researchers have found that head-down bed rest (HDBR) will affect the emotional state of individuals, and negative emotions such as anxiety are closely related to attention bias. The present study adopted the dot-probe task to evaluate the effects of 15-days of -6° HDBR on the attention bias of threatening stimulus in 17 young men, which was completed before (Pre-HDBR), during (HDBR-1, HDBR-8, HDBR-15), after (Post-HDBR) the bed rest. In addition, self-report inventories (State Anxiety Inventory, SAI; Positive Affect and Negative Affect Scale, PANAS) were conducted to record emotional changes.

Does the recent afforestation program in Ethiopia influenced vegetation cover and hydrology? A case study in the upper awash basin, Ethiopia.

The Ethiopian government planned afforestation programs in the past decades whereas more attention was given to tree plantation since the 2010 year. However, the effectiveness of the afforestation programs and its impacts on vegetation cover and hydrology has not been well studied. This study aims to assess the recent campaigned afforestation program and its impact on vegetation cover and hydrology in the upper Awash basin, Ethiopia.

Application of functional microbial agent in aerobic composting of wheat straw for waste recycling.

Controlled Ecological Life Support System (CELSS) is a closed artificial ecosystem which can regenerate oxygen, food, water and other substances for crew survival in long-term space missions. Solid organic waste is a vital resource pool for material reuse and recycling in CELSS. In this study, solid wastes including wheat straw and food waste were disposed via aerobic composting under functional microbial agent inoculation.

DNA Methylation Markers and Prediction Model for Depression and Their Contribution for Breast Cancer Risk.

Background: Major depressive disorder (MDD) has become a leading cause of disability worldwide. However, the diagnosis of the disorder is dependent on clinical experience and inventory. At present, there are no reliable biomarkers to help with diagnosis and treatment.

Lignocellulose degradation, biogas production and characteristics of the microbial community in solid-state anaerobic digestion of wheat straw waste.

Waste recycling is pivotal for deep space exploration or space habitation in life support systems (LSS) to enhance the material closure. This study investigated the enzymatic pretreatment and solid-state anaerobic digestion (SS-AD) of wheat straw as the major component of biomass waste in LSS for resource reclamation. Wheat straw compounds, such as cellulose, hemicellulose and lignin were significantly degraded after pretreatment with degradation at 37.

Effects of 15-Day Head-Down Bed Rest on Emotional Time Perception.

Accurate time perception is clearly essential for the successful implementation of space missions. To elucidate the effect of microgravity on time perception, we used three emotional picture stimuli: neutral, fear, and disgust, in combination with a temporal bisection task to measure 16 male participants' time perception in 15 days of -6° head-down bed rest, which is a reliable simulation model for most physiological effects of spaceflight. We found that: (1) participants showed temporal overestimation of the fear stimuli in the middle phase (day 8), suggesting that when participants' behavioral simulations were consistent with the action implications of the emotional stimuli, they could still elicit an overestimation of time even if the subjective arousal of the emotional stimuli was not high.

Systems Pharmacology-Based Precision Therapy and Drug Combination Discovery for Breast Cancer.

Breast cancer (BC) is a common disease and one of the main causes of death in females worldwide. In the omics era, researchers have used various high-throughput sequencing technologies to accumulate massive amounts of biomedical data and reveal an increasing number of disease-related mutations/genes. It is a major challenge to use these data effectively to find drugs that may protect human health.

Mapping Evapotranspiration of Agricultural Areas in Ghana.

Climate change is having an adverse effect on the environment especially in sub-Sahara Africa, where capacity for natural resource management such as water is very low. The scope of the effect on land use types have to be estimated to inform proper remedy. A combined estimation of transpiration and evaporation from plants and soil is critical to determine annual water requirement for different land use.

Operation overview of a biological waste treatment system during the 4-crew 180-day integrated experiment in the controlled ecological life support system (CELSS).

Waste management and treatment is vital to health care and material circulation, especially in the Controlled Ecological Life Support System (CELSS) with finite resources for long-duration manned space missions. A closed ecological-cycle integrated 4-crew 180-day experiment platform was established to investigate the key technologies such as effective cultivation of higher plant, water treatment and recycling, waste management and treatment. In this study, generated waste during the integrated experiment was classified as renewable and non-renewable waste.

Mining the Selective Remodeling of DNA Methylation in Promoter Regions to Identify Robust Gene-Level Associations With Phenotype.

Epigenetics is an essential biological frontier linking genetics to the environment, where DNA methylation is one of the most studied epigenetic events. In recent years, through the epigenome-wide association study (EWAS), researchers have identified thousands of phenotype-related methylation sites. However, the overlaps of identified phenotype-related DNA methylation sites between various studies are often quite small, and it might be due to the fact that methylation remodeling has a certain degree of randomness within the genome.

The large footprint of small-scale artisanal gold mining in Ghana.

Gold mining has played a significant role in Ghana's economy for centuries. Regulation of this industry has varied over time and while industrial mining is prevalent in the country, the expansion of artisanal mining, or Galamsey has escalated in recent years. Many of these artisanal mines are not only harmful to human health due to the use of Mercury (Hg) in the amalgamation process, but also leave a significant footprint on terrestrial ecosystems, degrading and destroying forested ecosystems in the region.

Temporal Characteristics of the Oropharyngeal and Nasal Microbiota Structure in Crewmembers Stayed 180 Days in the Controlled Ecological Life Support System.

Confined experiments are carried out to simulate the closed environment of space capsule on the ground. The Chinese Controlled Ecological Life Support System (CELSS) is designed including a closed-loop system supporting 4 healthy volunteers surviving for 180 days, and we aim to reveal the temporal characteristics of the oropharyngeal and nasal microbiota structure in crewmembers stayed 180 days in the CELSS, so as to accumulate the information about microbiota balance associated with respiratory health for estimating health risk in future spaceflight. We investigated the distribution of microorganisms and their dynamic characteristics in the nasal cavity and oropharynx of occupants with prolonged confinement.