Conferring non-strain-specific resistance to a potyvirus via overexpression of mutant potyviral coat proteins in soybean.

Transgenic soybean (Glycine max) plants expressing mutant potyviral coat proteins that disrupt virion assembly exhibited non-strain-specific resistance against soybean mosaic virus.

Transcriptional regulatory network reveals key transcription factors for regulating agronomic traits in soybean.

Background: Transcription factors (TFs) bind regulatory genomic regions to orchestrate spatio-temporal expression of target genes. Global dissection of the cistrome is critical for elucidating transcriptional networks underlying complex agronomic traits in crops.

Results: Here, we generate a comprehensive genome-wide binding map for 148 TFs using DNA affinity purification sequencing in soybean.

Global Gridded Crop Production Dataset at 10 km Resolution from 2010 to 2020.

The global gridded crop production dataset at 10 km resolution from 2010 to 2020 (GGCP10) for maize, wheat, rice, and soybean was developed to address limitations of existing datasets characterized by coarse resolution and discontinuous time spans. GGCP10 was generated using a series of adaptively trained data-driven crop production spatial estimation models integrating multiple data sources, including statistical data, gridded production data, agroclimatic indicator data, agronomic indicator data, global land surface satellite products, and ground data. These models were trained based on agroecological zones to accurately estimate crop production in different agricultural regions.

Understanding Uncertainty in Market-Mediated Responses to US Oilseed Biodiesel Demand: Sensitivity of ILUC Emission Estimates to GLOBIOM Parametric Uncertainty.

The life cycle greenhouse gas (GHG) emissions of biofuels depend on uncertain estimates of induced land use change (ILUC) and subsequent emissions from carbon stock changes. Demand for oilseed-based biofuels is associated with particularly complex market and supply chain dynamics, which must be considered. Using the global partial equilibrium model GLOBIOM, this study explores the uncertainty in market-mediated impacts and ILUC-related emissions from increasing demand for soybean biodiesel in the United States in the period 2020-2050.

Biodiesel Production from Soybean Oil Using a Free-Enzyme and Whole-Cell Dual Lipase System as a Biocatalyst.

A dual lipase system has been developed to convert soybean oil into biodiesel through synergistic catalysis of Thermomyces lanuginosus lipase (TLL) and Yarrowia lipolytica lipase 2 (YLL) in this study. Pichia pastoris recombinant strains expressing lipases were successfully constructed, and the activities of TLL and YLL in the fermentation supernatant reached 23,142.71 ± 280.

Combined Physiological and Transcriptomic Analysis Reveals Key Regulatory Networks and Potential Hub Genes Controlling Chilling Tolerance During Soybean Germination.

Chilling is an important limiting factor for seed germination of soybean ( [L.] Merr.).

Dataset for stability of high biomass and yield in maize under normal and intercropping conditions based on biplot, genotype stability index and land equivalent ratio.

Research on high-yielding and biomass of maize hibrids which adaptive to intercropping environment is important in the context of modern agriculture faced with the challenges of climate change. The field evaluation was conducted in Arjasari, West Java, Indonesia, for two seasons in three different cropping systems, namely: maize sole cropping, maize+soybean intercropping and maize+sweet potato intercropping. The evaluation applied a randomized completed block design with three replications.

Assessment of transglutaminase catalyzed cross-linking on the potential allergenicity and conformation of heterologous protein polymers.

Transglutaminase (TGase)-mediated cross-linking has gained significant attention due to its potential to reduce the allergenicity of food proteins. This study investigates the effects of TGase cross-linking on allergenicity and conformational modifications in a dual-protein system comprising soy protein isolate (SPI) and β-lactoglobulin (β-LG). The results showed that TGase cross-linking effectively decreased the allergenic potential of both SPI and β-LG, with a more pronounced reduction observed in the allergenicity of soy protein in the dual-protein system.

One Change, Many Benefits: A Glycine-Modified Bacteriochlorin with NIR Absorption and a Type I Photochemical Mechanism for Versatile Photodynamic Therapy.

Difluorinated sulfonamide porphyrin (FPGly) and bacteriochlorin (FBGly), modified by glycine residues, were synthesized and evaluated for photodynamic therapy (PDT). F₂PGly exhibits superior stability and singlet oxygen generation efficiency but features a low-intensity band in the red range (λ = 639 nm). In contrast, FBGly shows a favorable, red-shifted absorption spectrum (λ = 746 nm) that aligns well with phototherapeutic window, facilitating deeper tissue penetration.

Genome-Wide Association Study and Marker Development for Root Rot Resistance in Soybean.

root rot (FORR) is an important disease threatening soybean production. The development of marker-assisted selection (MAS) molecular markers will help accelerate the disease resistance breeding process and achieve the breeding goal of improving soybean disease resistance. This study evaluated the FORR disease resistance of 356 soybean germplasm accessions (SGAs) and screened resistance-related loci using genome-wide association analysis (GWAS) to develop molecular markers for MAS.

Validation and Optimization of a Stable Isotope-Labeled Substrate Assay for Measuring AGAT Activity.

L-arginine: glycine amidinotransferase (AGAT) gained academic interest as the rate-limiting enzyme in creatine biosynthesis and its role in the regulation of creatine homeostasis. Of clinical relevance is the diagnosis of patients with AGAT deficiency but also the potential role of AGAT as therapeutic target for the treatment of another creatine deficiency syndrome, guanidinoacetate N-methyltransferase (GAMT) deficiency. Applying a stable isotope-labeled substrate method, we utilized ARG 15N (ARG-δ2) and GLY 13C15N (GLY-δ3) to determine the rate of 1,2-13C,15N guanidinoacetate (GAA-δ5) formation to assess AGAT activity in various mouse tissue samples and human-derived cells.

From Fossil to Bio-Based AESO-TiO Microcomposite for Engineering Applications.

Environmental issues and the reduction of fossil fuel resources will lead to the partial or total substitution of petroleum-based materials with natural, raw, renewable ones. One expanding domain is the obtaining of engineering materials from vegetable oils for sustainable, eco-friendly polymers for different applications. Herein, the authors propose a simplified and green synthesis pathway for a thermally curable, acrylated and epoxidized soybean oil matrix formulation containing only epoxidized soybean oil, acrylic acid, a reactive diluent (5%) and just 0.

Studies on the Enzymatic Degradation Process of Epoxy-Polyurethane Compositions Obtained with Raw Materials of Natural Origin.

Along with the development of technology and the increasing consumption of polymeric materials, which have become an integral part of man's everyday life, problems related to their disposal are arising. The presented research concentrates on the studies on the enzymatic degradation of selected epoxy-polyurethane materials filled with 2 or 5 wt.% of waste unmodified or chemically modified through mercerization wood flour.

A Virulence Factor from Targets the Host Chloroplast Proteins to Promote Infection.

Chloroplasts are not only places for photosynthesis, but also participate in plant immunity and are important targets of pathogens. Pathogens secrete chloroplast-targeted proteins (CTPs) that disrupt host immunity and promote infection. (Lib.

Comparing Results from 2-D and 3-D Phenotyping Systems for Soybean Root System Architecture: A 'Comparison of Apples and Oranges'?

Typically, root system architecture (RSA) is not visible, and realistically, high-throughput methods for RSA trait phenotyping should capture key features of developing root systems in solid substrates in 3D. In a published 2-D study using thin rhizoboxes, vermiculite as a growing medium, and photography for imaging, triplicates of 137 soybean cultivars were phenotyped for their RSA. In the transition to 3-D work using X-ray computed tomography (CT) scanning and mineral soil, two research questions are addressed: (1) how different is the soybean RSA characterization between the two phenotyping systems; and (2) is a direct comparison of the results reliable? Prior to a full-scale study in 3D, we grew, in pots filled with sand, triplicates of the Casino and OAC Woodstock cultivars that had shown the most contrasting RSAs in the 2-D study, and CT scanned them at the V1 vegetative stage of development of the shoots.

Highly Efficient Mediated Transformation of Oil Palm Using an -Glyphosate Selection System.

Oil palm ( Jacq.) is the most efficient oil-producing crop globally, yet progress in its research has been hampered by the lack of effective genetic transformation systems. The gene, encoding 5-enolpyruvylshikimate-3-phosphate synthase, has been used as a transgenic selection marker in various crops, including rice and soybean.

Fabrication and Encapsulation of Soy Peptide Nanoparticles Using Ultrasound Followed by Spray Drying.

Peptide aggregation inevitably occurs during hydrolysis, and insoluble peptide aggregates (ISPA) are used as feed for animals due to their poor water solubility and unpleasant bitter flavor. Ultrasound was used to fabricate soy peptide nanoparticles by reassembling ISPA, followed by spray-drying encapsulation to develop low-bitterness peptide microcapsules with soluble soybean polysaccharide (SSPS) and stevioside (STE) as wall materials. Powder properties, bitter taste, and the morphology of the microcapsules were evaluated.

Effect of Pea Protein Isolate-Soybean Meal Ratio on Fiber Structure and Texture Properties of High-Moisture Meat Analogs.

Inadequate fibrous attributes and prohibitive pricing are pivotal barriers to the broader market penetration of meat analogs (MAs). This research endeavors to address these impediments by formulating a blend of cost-effective soybean meal (SM) and pea protein isolate (PPI) across a spectrum of ratios (PPI:SM = 1:0, 8:2, 6:4, 4:6, 2:8, and 0:1). The analysis of textural properties elucidated that the integration of SM markedly diminished the textural rigidity and mastication resistance of MAs.

Effects of β-Mannanase Alone or Combined with Multi-Carbohydrase Complex in Corn-Soybean Meal Diets on Nutrient Metabolism and Gut Health of Growing Pigs.

(1) Background: This study was performed to evaluate whether the addition of β-mannanase alone or combined with a multi-carbohydrase complex can improve diet digestibility, nutrient and energy metabolism, and the gut health of growing pigs. (2) Methods: Twenty-four pigs (35.56 ± 3.

Compositional Variations in Wheat Bran Influence Growth Performance, Nutrient Retention, and Cecal Microbiome in Broilers.

Recent studies have discovered that wheat bran could stimulate digestive tract development, modulate intestinal microbiota, and improve the digestibility of fibrous ingredients in broilers. Wheat bran varies greatly in its chemical constituents. The objective of this study is to investigate how different types of wheat bran influence growth performance, nutrient digestion, and gut health in broiler chickens.

Effects of Extrusion on the Available Energy and Nutrient Digestibility of Soybean Meal and Its Application in Weaned Piglets.

Experiments were conducted to investigate the effects of extrusion devices on the available energy and nutrient digestibility of soybean meal (SBM), and further to investigate the impact of different levels of extruded SBM on the growth performance and nutrient digestibility of weaned piglets. In Exp. 1, eighteen crossbreed growing pigs with an initial body weight (BW) of 32.

Molybdenum Disulfide/Diselenide-Laser-Induced Graphene-Glycine Oxidase Composite for Electrochemical Sensing of Glyphosate.

The widespread use of the pesticide glyphosate has raised concerns regarding its potential health and environmental impacts. Consequently, there is an increasing demand for monitoring glyphosate levels in surface waters and food products. Currently, there is no commercially available rapid, field-deployable sensor capable of quantifying glyphosate concentrations in environmental samples.