An animal model recapitulates human hepatic diseases associated with mutations.

Heterozygotic mutations are responsible for various congenital diseases in the heart, pancreas, liver, and other organs in humans. However, there is lack of an animal that can comprehensively model these diseases since GATA6 is essential for early embryogenesis. Here, we report the establishment of a knockout zebrafish which recapitulates most of the symptoms in patients with mutations, including cardiac outflow tract defects, pancreatic hypoplasia/agenesis, gallbladder agenesis, and various liver diseases.

Nano-Armed Limosilactobacillus reuteri for Enhanced Photo-Immunotherapy and Microbiota Tryptophan Metabolism against Colorectal Cancer.

Despite being a groundbreaking approach to treating colorectal cancer (CRC), the efficacy of immunotherapy is significantly compromised by the immunosuppressive tumor microenvironment and dysbiotic intestinal microbiota. Here, leveraging the superior carrying capacity and innate immunity-stimulating property of living bacteria, a nanomedicine-engineered bacterium, LR-S-CD/CpG@LNP, with optical responsiveness, immune-stimulating activity, and the ability to regulate microbiota metabolome is developed. Immunoadjuvant (CpG) and carbon dot (CD) co-loaded plant lipid nanoparticles (CD/CpG@LNPs) are constructed and conjugated to the surface of Limosilactobacillus reuteri (LR) via reactive oxygen species (ROS)-responsive linkers.

Transgenic overexpression of UDP glycosyltransferase gene UGT41A3 induces resistance to nucleopolyhedrovirus in Bombyx mori.

Bombyx mori nuclear polyhedrosis, caused by B. mori nucleopolyhedrovirus (BmNPV), threatens sericulture seriously. To explore strategies for controlling it, the UDP glycosyltransferase gene UGT41A3 (BmUGT41A3) was targeted.

Priority attribute algorithm for Q-matrix validation: A didactic.

The Q-matrix is one of the core components of cognitive diagnostic assessment, which is a matrix describing the relationship between items and the attributes being assessed. Numerous studies have shown that inaccuracies in defining the Q-matrix can degrade parameter estimation and model fitting results. Currently, Q-matrix validation often involves exhaustive search algorithms (ESA), which traverse through all possible -vectors and determine the optimal -vector for items based on indicators or criteria corresponding to different validation methods.

The reciprocal relationship between social exclusion and basic psychological needs through cross-lagged analysis.

Social exclusion has wide-ranging and detrimental effects. This study recruited 771 Chinese college students (Mage = 19.65 years, SDage = 1.

Attenuated cadmium and arsenic enrichment in rice by co-application of organic composting and chemical fertilization.

A pot experiment was conducted on arsenic (As) and cadmium (Cd) co-contaminated soil to discern the influence of varying proportions of pig manure compost (PM) vis-à-vis chemical fertilizers (NPK) on the mitigation of Cd and As absorption by rice. Our findings illustrated that by increasing the PM proportions from 25 to 100%, it manifested a statistically significant reduction in the mobilized fractions of Cd, accounting for up to 77% reduction in soil CaCl-Cd concentrations. Conversely, the NaHCO-As reactions were contingent on the distinct PM application rates.

Optimal exercise dose-response improves health-related quality of life in cancer survivors: a systematic review and Bayesian network meta-analysis of RCTs.

Objective: Cancer survivors often face significant health-related quality of life (HRQoL) challenges. Although exercise has been proven to improve HRQoL in cancer survivors, the optimal dose and intensity of exercise for this population has not been fully determined. Adherence to exercise may vary based on exercise intensity, affecting results.

Validation of the I- and D-type epistemic curiosity scale among young Chinese children and implications on early curiosity nurture.

Curiosity, an innate and intrinsic motivation to explore, makes vital contributions to learning in individuals of various ages. Epistemic curiosity centers on the drive to close information gaps and can be classified into joyous exploration and interest (I) and deprivation sensitivity (D) types. Each subtype is associated with different academic achievements, personality traits, emotions, and aspects of creativity.

Integrated comparative physiological and transcriptomic analyses of Elymus sibiricus L. reveal the similarities and differences in the molecular mechanisms in response to drought and cold stress.

Drought and cold crucially affect plant growth and distribution. Plants have evolved complex molecular mechanisms to adapt to such adverse environmental conditions. This study examines two Elymus sibiricus (Es) germplasms differing in resilience to these stresses.

STLBRF: an improved random forest algorithm based on standardized-threshold for feature screening of gene expression data.

When the traditional random forest (RF) algorithm is used to select feature elements in biostatistical data, a large amount of noise data and parameters can affect the importance of the selected feature elements, making the control of feature selection difficult. Therefore, it is a challenge for the traditional RF algorithm to preserve the accuracy of algorithm results in the presence of noise data. Generally, directly removing noise data can result in significant bias in the results.

Study protocol: A national cross-sectional study on psychology and behavior investigation of Chinese residents in 2023.

Introduction: This study protocol specifies the primary research line and theoretical framework of the 2023 Survey of the Psychology and Behavior of the Chinese Population. It aims to establish a consistent database of Chinese residents' psychological and behavioral surveys through multi-center and large-sample cross-sectional surveys to provide robust data support for developing research in related fields. It will track the public's physical and psychological health more comprehensively and systematically.

Association between breakfast consumption frequency and handgrip strength and standing long jump: a systematic review and meta-analysis.

Background And Aims: Over the past decade, numerous studies investigating the relationship between breakfast consumption frequency and handgrip strength and standing long jump have produced conflicting evidence, leading to uncertainty regarding this association. This study aimed to provide further clarity on the relationship between breakfast consumption frequency and handgrip strength and standing long jump.

Methods: A comprehensive search of the literature up to September 15, 2023, was conducted on Web of Science, PubMed, Scopus, MEDLINE, and CNKI.

Bio-energy-powered microfluidic devices.

Bio-microfluidic technologies offer promising applications in diagnostics and therapy, yet they face significant technical challenges, particularly in the need for external power sources, which limits their practicality and user-friendliness. Recent advancements have explored innovative methods utilizing body fluids, motion, and heat to power these devices, addressing the power supply issue effectively. Among these, body-motion and body-heat-powered systems stand out for their potential to create self-sustaining, wearable, and implantable devices.

Carbon sinks associated with biological carbon pump in karst surface waters: progress, challenges, and prospects.

The biological carbon pump (BCP) associated with aquatic photosynthesis in karst surface waters converts dissolved inorganic carbon (DIC) into organic carbon. In the context of global climate change, BCP could be an important carbon sink mechanism, ultimately regulating atmospheric carbon dioxide (CO) and mitigating climate change. Because of the high DIC and pH, and low dissolved CO [CO (aq)], the hydrochemical characteristics of karst surface water bodies cause C limitation in BCP efficiency.

Low background catalytic redox recycling coupled with hybridization chain reaction amplification for highly sensitive electrochemical aptamer luteinizing hormone assay.

The concentration variation of luteinizing hormone (LH) regulates the cell cycle of oocyte meiosis and significantly affect the whole reproductive cycle. Sensitively quantifying the LH biomarker therefore plays an important role for reproductive disease diagnosis. By coupling a new low background catalytic redox recycling strategy with hybridization chain reaction (HCR), we propose a highly sensitive bio-electrochemical aptamer LH sensing method.

Clean and sustainable extraction of gelatin: Effects of microwave and freeze-thaw on the crosslinking degree and hydrogen bond of fish skin collagen.

The traditional gelatin extraction methods (acid-base) may hinder to their green applications due to mass energy consumption and pollution. Herein, we constructed a clean and sustainable gelatin extraction method, investigated the molecular mechanism of microwave treatment (0-360 min) and freeze-thaw on the gelatin extraction from the perspective of the crosslinking degree and hydrogen bonds. Microwave (0-60 min) can improve the hydrolysis degree (DH) and expose more enzyme cleavage sites of collagen by destroying the intramolecular and intermolecular covalent crosslinking.

Machine learning combined with geodetector to predict the spatial distribution of soil heavy metals in mining areas.

An accurate understanding of the spatial distribution of soil heavy metals (HMs) is crucial for the effective prevention of soil pollution and remediation strategies. Traditional machine learning models often overlook the spatially stratified heterogeneity inherent to environmental data, which can impair predictive accuracy. Therefore, we combined the Geodetector model (GDM) with machine learning models.

Photothermal-manipulatable shape memory polyacrylamide/gelatin Janus hydrogel with drug carrier array for invasive wound closure and responsive drug release.

Traditional wound closure methods often present several issues, including additional puncture wounds, adverse effects from anesthesia, and noticeable scarring. Inspired by embryonic wound healing, a Janus hydrogel (PG/Au-Asp@PCM) is designed to manipulate non-invasive wound closure by photothermal-responsive self-contraction of PG/Au-Asp@PCM, which is attributed to the shape memory behavior of PG/Au-Asp@PCM under near-infrared (NIR). Wherein, gelatin acts as a thermally reversible "switch" and polyacrylamide creates stable and cross-linked "net-points".

The glutathione S-transferase BnGSTU12 enhances the resistance of Brassica napus to Sclerotinia sclerotiorum through reactive oxygen species homeostasis and jasmonic acid signaling.

Sclerotinia sclerotiorum is a severe disease that affects rapeseed (Brassica napus), resulting in significant yield losses. In previous study, we identified the candidate GLUTATHIONE S-TRANSFERASE (GST) gene, BnGSTU12, associated with sclerotiorum stem resistance and the expression levels of BnGSTU12 in resistant lines were higher than that in susceptible lines. We analyzed the function of the BnGSTU12 during S.

Enantioselectivity Mechanism of Etoxazole Enantiomers in : Acaricidal Activity, Metabolic Difference, and Target Binding.

Etoxazole, a widely used mite growth inhibitor, contains a chiral center in its chemical structure, resulting in two mirror-image enantiomers. These enantiomers of etoxazole display significant differences in biological activity and environmental behavior. In bioassays conducted against , it was observed that S-etoxazole demonstrated approximately 279.

The potential role of vesicle transport-related small GTPases rabs in abiotic stress responses.

Rab GTPases are a class of small GTP-binding proteins, play crucial roles in the membrane transport machinery with in eukaryotic cells. They dynamically regulate the precise targeting and tethering of transport vesicles to specific compartments by transitioning between active and inactive states. In plants, Rab GTPases are classified into eight distinct subfamilies: Rab1/D, Rab2/B, Rab5/F, Rab6/H, Rab7/G, Rab8/E, Rab11/A, and Rab18/C.

Transcriptome-derived evidence reveals the regulatory network in the skeletal muscle of the fast-growth mstnb male tilapia.

Myostatin (Mstn) negatively regulates muscle growth and Mstn deficiency induced "double-skeletal muscle" development in vertebrates, including tilapias. In this study, we performed a transcriptomic analysis of skeletal muscle from both wild-type and mstnb males to investigate the molecular mechanisms underlying skeletal muscle hypertrophy in mstnb mutants. We identified 4697 differentially expressed genes (DEGs), 113 differentially expressed long non-coding RNAs (DE lncRNAs), 211 differentially expressed circular RNAs (DE circRNAs), and 98 differentially expressed microRNAs (DE miRNAs).

Automated identification of impact spatters and fly spots with a residual neural network.

In criminal investigations, distinguishing between impact spatters and fly spots presents a challenge due to their morphological similarities. Traditional methods of bloodstain pattern analysis (BPA) rely significantly on the expertise of professional examiners, which can result in limitations including low identification efficiency, high misjudgment rates, and susceptibility to external disturbances. To enhance the accuracy and scientific rigor of identifying impact spatters and fly spots, this study employed artificial intelligence techniques in image recognition and transfer learning.