Isolation and purification of carbohydrate components in functional food: a review.

Medicinal plants, increasingly utilized in functional foods, possess potent therapeutic properties and health-promoting functions, with carbohydrates playing a crucial role and exhibiting a range of effects, such as antioxidant, antitumor, immune-enhancing, antibacterial, anticoagulant, and hypoglycemic activities. However, comprehensively, accurately, rapidly, and economically assessing the quality of carbohydrate components is challenging due to their diverse and complex nature. Additionally, the purification and identification of carbohydrates also guarantee related efficacy research.

sp. nov., isolated from baijiu fermented grains, could transform ginsenosides of .

A Gram-stain-positive actinomycete, designated REN17, was isolated from fermented grains of Baijiu collected from Sichuan, PR China. It exhibited branched substrate mycelia and a sparse aerial mycelium. The optimal growth conditions for REN17 were determined to be 28 °C and pH 7, with a NaCl concentration of 0 % (w/v).

Application Progress of Culturomics in the Isolated Culture of Rhizobacteria: A Review.

Comprehending the structure and function of rhizobacteria components and their regulation are crucial for sustainable agricultural management. However, obtaining comprehensive species information for most bacteria in the natural environment, particularly rhizobacteria, presents a challenge using traditional culture methods. To obtain diverse and pure cultures of rhizobacteria, this study primarily reviews the evolution of rhizobacteria culturomics and associated culture methods.

Resuscitation of baijiu pit mud bacteria based on Rpf protein of Umezawaea beigongshangensis.

The pit mud in the Baijiu fermentation cellar is an abundant microbial resource that is closely related to the quality of baijiu. However, many naturally existing species might be in a viable but nonculturable (VBNC) state, posing challenges to the isolation and application of functional species. Herein, a previously isolated strain from baijiu mash, Umezawaea beigongshangensis, was found to contain the rpf gene that encodes resuscitation promotion factor (Rpf).

Simultaneous Determination of 147 Pesticide Residues in Traditional Chinese Medicines by GC-MS/MS.

Determination of pesticide residues remains a challenge in traditional Chinese medicines in which complex compounds may interfere with analysis signals. This study reports the development of a simple, effective, and high-throughput method combining gas chromatography-tandem mass spectrometry (GC-MS/MS) with either QuEChERS or solid phase extraction (SPE) to determine 147 pesticide residues in traditional Chinese medicines simultaneously. In SPE, the mixture of -hexane and ethyl acetate (1:1, v/v) was selected to extract 147 pesticides in honeysuckle, and the extracted pesticides were determined by GC-MS/MS.

Determination of Dencichine in in the Forest and Field Using High Performance Liquid Chromatography.

Dencichine is a nonprotein amino acid, an effective ingredient in with hemostatic and anti-inflammatory effects. There are few studies on the effects of regions and cultivation models on the accumulation of dencichine. In the current study, the content of dencichine in collected from its global cultivation and trading center Yunnan, China, (>640 samples) was determined using an optimized high-performance liquid chromatography method coupled with a diode array detector but without derivatization.

Interaction of Lead and Cadmium Reduced Cadmium Toxicity in Seedlings.

Potentially toxic elements (PTEs) pollution occurs widely in soils due to various anthropogenic activities. Lead (Pb) and cadmium (Cd) coexist in soil frequently, threatening plant growth. To explore the interaction effect between Pb and Cd in and the response of plant physiological characteristics to Pb and Cd stress, we designed a soil culture experiment.

Development of an automated solid phase extraction instrument for determination of lead in high-salt foods.

In this study, an instrument for solid phase extraction of heavy metals was developed. Compared to conventional separation equipment, it has advantages of small size, less reagent consumption, time saving, easy operation, and good reproducibility. After analyzing the partition coefficient and adsorption capacity of the exchange resin, the Chelex-100 resin achieved a strong adsorption of lead at pH of 6.

Long-term continuous mono-cropping of greatly affects soil physicochemical properties, rhizospheric bacterial diversity, and metabolite contents.

is the most economically important Proteaceae crop known for its edible nuts. The present study was conducted to examine the effect of continuous cultivation (for 1, 5, and 20 years) of on soil quality, bacterial diversity, and metabolites. Soil rhizospheres from three different rhizosphere orchards, 1-year monoculture orchard (CK), 5-year monoculture orchard (Y5), and 20-year monoculture orchard (Y20), were analyzed through metagenomic and metabolomic profiling.

Determination of the Authenticity and Origin of Panax Notoginseng: A Review.

Panax notoginseng, a traditional medicinal and edible plant, is widely used in medicine, health care, cosmetics, and other industries. Affected by the discrepancy between market supply and demand and price, the adulteration of P. notoginseng products with other plant-derived ingredients occurs at times.

Rapid screening and quantification of heavy metals in traditional Chinese herbal medicines using monochromatic excitation energy dispersive X-ray fluorescence spectrometry.

Traditional Chinese herbal medicines are subject to heavy metal contamination. Standard detection methods are too complicated, time-consuming, and expensive for routine analysis, so low-cost methods are in high demand for rapid on-site screening. This study reports a high-sensitivity X-ray fluorescence (HS-XRF) method to determine As, Pb, and Cd residues simultaneously in herbal medicines.

Comparative Analysis of the Floral Fragrance Compounds of Flowers under the - Agroforestry System Using SPME-GC-MS.

is a medicinal plant in China, the flowers of which have high medicinal value. To study the differences in the floral fragrance compounds of flowers (bionic wild cultivation) from the forests of Yunnan Province, the floral fragrance compounds from four varieties of flowers (four-forked seven leaves, three-forked seven leaves, four-forked five-seven leaves, and three-forked five-six leaves) were compared and analyzed via headspace solid phase microextraction combined with gas chromatography-mass spectrometry methods. A total of 53 floral fragrance compounds from the flowers were divided into eight categories, mainly consisting of terpenes, alkynes, aromatic hydrocarbons, and alcohols.

Multi-Element Analysis and Origin Discrimination of Based on Inductively Coupled Plasma Tandem Mass Spectrometry (ICP-MS/MS).

is an important functional health product, and has been used worldwide because of a wide range of pharmacological activities, of which the taproot is the main edible or medicinal part. However, the technologies for origin discrimination still need to be further studied. In this study, an ICP-MS/MS method for the accurate determination of 49 elements was established, whereby the instrumental detection limits (LODs) were between 0.

Combined Transcriptome and Lipidomic Analyses of Lipid Biosynthesis in Nuts.

Macadamia nuts are considered a high-quality oil crop worldwide. To date, the lipid diversity and the genetic factors that mediate storage lipid biosynthesis in are poorly known. Here, we performed a comprehensive transcriptomic and lipidomic data analysis to understand the mechanism of lipid biosynthesis by using young, medium-aged, and mature fruit kernels.

The complete chloroplast genome sequence of Rolfe, a vulnerable wild moth orchid species (Orchidaceae).

Rolfe is a vulnerable wild moth orchid species with important horticultural value. The complete chloroplast genome sequence of was generated by de novo assembly using whole genome next-generation sequencing to provide genomic data for further conservation genetics, phylogeny and molecular breeding in . The complete plastome of is 145,096 bp in length, containing two inverted repeats (IR) regions (24,787 bp), a large single-copy (LSC) region (84,688 bp), and a small single-copy (SSC) region (10,834 bp).

The complete chloroplast genome of Rehder (Hydrangeaceae).

Rehder is a wild flowering shrub with high ornamental value. The complete chloroplast genome sequence of was characterized from Hiseq (Illumina Co., San Diego, CA) sequencing data.

Polysaccharides and polyphenol in dried fruit tea after different processing conditions: Optimization analysis using response surface methodology.

The increasing popularity of has many medical and health benefits because of its rich polysaccharides (PSC) and polyphenols (PPN). It has become popular to brew the dry fruit slice as tea in some regions of China. In this study, optimize the extraction parameters of tea polysaccharides and polyphenols using response surface methodology.

[Effects of exogenous abscisic acid (ABA) on growth and physiological characteristics of Machilus yunnanensis seedlings under drought stress].

In this study, the effects of exogenous abscisic acid (ABA) on the growth and physiolo-gical characteristics of Machilus yunnanensis seedlings were examined under well water supply conditions (70%-75% field capacity, FC) and different drought stress conditions, i.e., light drought stress (50%-55% FC) and severe drought stress (30%-35% FC), respectively.