1 results match your criteria: "South Manchester University Hospitals National Health Service Trust[Affiliation]"
Quantification of urinary oxalate by liquid chromatography-tandem mass spectrometry with online weak anion exchange chromatography.
Clin Chem
December 2006
Department of Clinical Biochemistry, Wythenshawe Hospital, South Manchester University Hospitals National Health Service Trust, Manchester, United Kingdom.
Background: Urinary oxalate is commonly measured with an enzymatic assay that is specific but requires a manual clean-up step to reduce ascorbic acid interference. We developed a urinary oxalate assay that uses liquid chromatography-tandem mass spectrometry (LC-MS/MS) with anion exchange chromatography and simple sample preparation.
Methods: We added calibrator or urine sample (10 microL) to 10 microL of (13)C2 oxalate and 400 microL of water and performed separation on a Waters OASIS WAX column, flow rate 0.