2 results match your criteria: "South Carolina 29425 and Ralph H. Johnson Veterans Administration Medical Center[Affiliation]"
Assessment of ToxCast Phase II for Mitochondrial Liabilities Using a High-Throughput Respirometric Assay.
Toxicol Sci
August 2015
*MitoHealth Inc., Charleston, South Carolina, Department of Drug Discovery and Biomedical Sciences, Medical University of South Carolina, Charleston, South Carolina 29425 and
Previous high-throughput screens to identify mitochondrial toxicants used immortalized cell lines and focused on changes in mitochondrial membrane potential, which may not be sufficient and do not identify different types of mitochondrial dysfunction. Primary cultures of renal proximal tubule cells (RPTC) were examined with the Seahorse Extracellular Flux Analyzer to screen 676 compounds (5 μM; 1 h) from the ToxCast Phase II library for mitochondrial toxicants. Of the 676 compounds, 19 were classified as cytotoxicants, 376 were electron transport chain (ETC) inhibitors, and 5 were uncouplers.
View Article and Find Full Text PDFUrinary ATP Synthase Subunit β Is a Novel Biomarker of Renal Mitochondrial Dysfunction in Acute Kidney Injury.
Toxicol Sci
May 2015
*Department of Drug Discovery and Biomedical Sciences, Medical University of South Carolina, Charleston, South Carolina 29425, Department of Medicine, Division of Nephrology, Medical University of South Carolina, Charleston, South Carolina 29425 and Ralph H. Johnson Veterans Administration Medical Center, Charleston, South Carolina 29425 *Department of Drug Discovery and Biomedical Sciences, Medical University of South Carolina, Charleston, South Carolina 29425, Department of Medicine, Division of Nephrology, Medical University of South Carolina, Charleston, South Carolina 29425 and Ralph H. Johnson Veterans Administration Medical Center, Charleston, South Carolina 29425
Article Synopsis
- Mitochondrial dysfunction is crucial in acute kidney injury (AKI), and the study investigated urinary ATP synthase subunit β (ATPSβ) as a noninvasive biomarker for this issue.
- The analysis showed that urinary ATPSβ levels increased significantly in mice with more severe ischemia and correlated with signs of mitochondrial dysfunction and renal injury, lasting up to 72 hours post-reperfusion.
- In human subjects, higher urinary ATPSβ levels were also found in patients experiencing AKI after cardiac surgery, suggesting its potential use as a sensitive biomarker for evaluating renal health and therapeutic interventions.