The spatially informed mFISHseq assay resolves biomarker discordance and predicts treatment response in breast cancer.

Current assays fail to address breast cancer's complex biology and accurately predict treatment response. On a retrospective cohort of 1082 female breast tissues, we develop and validate mFISHseq, which integrates multiplexed RNA fluorescent in situ hybridization with RNA-sequencing, guided by laser capture microdissection. This technique ensures tumor purity, unbiased whole transcriptome profiling, and explicitly quantifies intratumoral heterogeneity.

What are the hidden shortcomings of balance training research in older adults that prevent its transfer into practice? Scoping review.

Background: Although a lot of attention is paid to the flaws of balance training research in older adults, the low methodological quality and incomplete reporting of studies still limit the knowledge transfer between research and practice. These known shortcomings are considered also as barriers for creating recommendations for balance training in older adults. Despite the considerable efforts to improve the scientific quality of studies, such recommendations have not yet been formulated to date.

Transplant and Nontransplant Salvage Therapy in Pediatric Relapsed or Refractory Hodgkin Lymphoma: The EuroNet-PHL-R1 Phase 3 Nonrandomized Clinical Trial.

Importance: The current standard-of-care salvage therapy in relapsed/refractory classic Hodgkin lymphoma (cHL) includes consolidation high-dose chemotherapy (HDCT)/autologous stem cell transplant (aSCT).

Objective: To investigate whether presalvage risk factors and fludeoxyglucose-18 (FDG) positron emission tomography (PET) response to reinduction chemotherapy can guide escalation or de-escalation between HDCT/aSCT or transplant-free consolidation with radiotherapy to minimize toxic effects while maintaining high cure rates.

Design, Setting, And Participants: EuroNet-PHL-R1 was a nonrandomized clinical trial that enrolled patients younger than 18 years with first relapsed/refractory cHL across 68 sites in 13 countries in Europe between January 2007 and January 2013.

A theoretical study on the environmental oxidation of fenpyrazamine fungicide initiated by hydroxyl radicals in the aqueous phase.

Fenpyrazamine (FPA) is a widely used fungicide in agriculture to control fungal diseases, but its environmental degradation by oxidants and the formation of potential degradation products remain unexplored. This study investigates the oxidation of FPA by hydroxyl radicals (HO˙) using density functional theory (DFT) calculations at the M06-2X/6-311++G(3df,3pd)//M06-2X/6-31+G(d,p) level of theory. Three standard oxidation mechanisms, including formal hydrogen transfer (FHT), radical adduct formation (RAF), and single electron transfer (SET), were evaluated in the aqueous phase, with reaction kinetics analyzed over a temperature range of 283-333 K.

Non-persistence with multiple secondary prevention medications for peripheral arterial disease among older hypertensive patients.

Introduction: The benefit of secondary prevention in hypertensive patients with peripheral arterial disease (PAD) is based on continual simultaneous taking of statins, antiplatelet agents and antihypertensive agents, preferably angiotensin-converting enzyme inhibitors (ACEIs) or angiotensin receptor blockers (ARBs). Our study was aimed at a) the analysis of the extent of non-persistence with multiple medication classes, and b) identifying factors associated with the likelihood of non-persistence.

Methods: In our cohort study, 3,401 hypertensive patients (1,853 females and 1,548 males) aged ≥65 years treated simultaneously with statins, antiplatelet agents and ACEIs/ARBs and in whom PAD was newly diagnosed during 2012 were analysed.

Quality of life in seniors living in home environment in Poland and Slovakia.

Introduction And Objective: Poor motor skills can increase the burden of disease and negatively affect the overall quality of life. The aim of the study was to investigate how people aged 60 and over assess their overall quality of life, overall health status and other domains of life in relation to socio-demographic factors.

Material And Methods: The study was conducted among 1,534 people aged 60 and over and living in a home environment - 831 people living in Małopolskie Province in Poland, and 703 people from Prešov Province in Slovakia.

PEG-Polymeric Nanocarriers Alleviate the Immunosuppressive Effects of Free 4-Thiazolidinone-Based Chemotherapeutics on T Lymphocyte Function and Cytokine Production.

Purpose: Our study aimed to assess the effects of anticancer 4-thiazolidinone-based free water-insoluble therapeutics Les-3288 and Les-3833 and their waterborne complexes with branched PEG-containing polymeric carriers (A24-PEG550 and A24-PEG750) on immune response.

Methods: Human peripheral blood was used to study in vitro lymphocyte proliferative function, leukocyte phagocytic activity and respiratory burst, and cytokine production.

Results: The binding of the polymer to the anticancer drug Les-3288, which is intended to mitigate the immunosuppressive effects of the free drug on the proliferative activity of T lymphocytes and T-dependent B cells, demonstrated comparable efficacy for both A24-PEG750 and A24-PEG550 nanocarriers.

In Search of Candidate Protein Biomarkers Related to COVID-19 in Solid Tissues and Non-Blood Fluids: An Update.

Purpose: During COVID-19, significant changes in protein abundance can be linked with disease-related processes. The mass spectrometry-based proteomics of COVID-19-related biomarkers can help with the prognosis and diagnosis of this severe disease.

Design: Here, we surveyed scientific works in terms of proteomic analysis of solid tissues and non-blood fluids from COVID-19 patients.

Camera and telemetry data from Slovak roads in various light and weather conditions.

In this paper, we describe the dataset captured with our proprietary data capture solution mounted on top of a Land Rover Defender vehicle. The captured data are the real data of drives on various Slovak roads. The total dataset consist of almost 33 hours of driving with a automotive grade FPD Link camera with 30 fps and with additional sensors such as high-precision GNSS sensor and modem towards mobile data connectivity LTE and 5 G.

Essential oil of (L.) ssp. (Apiaceae) flower: chemical composition, antimicrobial potential, and insecticidal activity on (L.).

In order to search for new chemotypes and to carry out a comparative study with the literature, the current study investigated the chemical composition of the essential oil of the flowers of (L.) ssp. using gas chromatography coupled with mass spectrometry (GC-MS).

Gene expression & biochemical analysis in alkaptonuria caused by a founder pathogenic variant across different age groups from India.

Background & objectives Alkaptonuria (AKU) is an autosomal recessive disease wherein biallelic pathogenic variants in the homogentisate 1,2- dioxygenase (HGD) gene encoding the enzyme homogentisate 1,2 dioxygenase cause high levels of homogentisic acid (HGA) to circulate within the body leading to its deposition in connective tissues and excretion in urine. A homozygous splice donor variant (c.87+1G>A) has been identified to be the founder variant causing alkaptonuria among Narikuravars, a group of gypsies settled in Tamil Nadu.

Tooth loss, periodontal infection and their relationship to cognitive impairment and other dementias: A review.

Our review study addresses the issue of tooth loss, which is caused by loss of masticatory function and its impact on cognitive functions, dementia, and Alzheimer's disease. Numerous studies have confirmed a positive correlation between premature tooth loss, reduction in masticatory function and significant cognitive decline observed through learning disabilities, including overcoming ordinary life problems to early and advanced forms of dementia. Reduced numbers of teeth in the main food processing area, i.

Validation of parental screening tool S-PMV11: Convergent validity with Bayley-III in assessing developmental functioning.

Background: With the increasing use of developmental screening tools, there is a growing need to validate parental screening methods for the early detection of developmental difficulties in children, regarding their psychometric properties.

Methods: This study evaluates the convergent validity of the S-PMV11 parental screening tool by comparing its outcomes with the Bayley Scales of Infant and Toddler Development (Bayley-III), the gold standard for direct assessment.

Results: We analyzed data from 30 children and found significant correlations between S-PMV11 scores and Bayley-III assessments across cognitive, language, and motor skill domains.

Disease characteristics and cognitive impairment in multiple sclerosis: A short-term observation is not enough.

Background: Multiple sclerosis (MS) is a disease that affects the central nervous system. One of its manifestations is cognitive impairment (CI), which can negatively affect the quality of life in people with MS (pwMS). This study aimed to investigate the nature of CI in MS and its associations with various disease characteristics.

Morphometric assessment of the choroid in dogs diagnosed with retinal atrophy (RA) with symptoms of progressive retinal atrophy, using spectral-domain optical coherence tomography (SD-OCT).

The aim of the study was to determine the thickness of choroidal layers in mixed breed dogs suffering from retinal atrophy (RA) and showing symptoms of progressive retinal atrophy (PRA), with the use of SD-OCT. The study was performed on 50 dogs divided into two groups: 25 dogs diagnosed with retinal atrophy (RA) with PRA symptoms aged 1.5-14 years and 25 healthy dogs aged 2-12 years.

Effect of selected bacteria of the genus Pseudomonas on the quality of raw cow's milk.

Pseudomonas spp. are a psychrotrophic species associated with milk spoilage caused by its enzymatic activities. The aim of this study was to identify Pseudomonas spp.

Carriage of antimicrobial resistance genes in Escherichia coli of bovine origin.

The present study aimed to search for the presence of the plasmid-mediated antimicrobial resistance genes in 106 Escherichia coli (E. coli) isolates from a total of 240 fresh fecal samples collected from 12 private cattle farms in Bingol province of East Turkey from November 2021 to January 2022. In those colistin-resistant E.

Two decades of research on sensu lato in questing ticks in Slovakia.

Introduction: In Europe sensu lato (s.l.), the causative agent of Lyme borreliosis is transmitted by the castor bean tick, .

Effects of high-frequency repetitive transcranial magnetic stimulation of affected hemisphere on motor recovery in patients in the acute stage of ischemic stroke: Preliminary results .

Objectives: Repetitive transcranial magnetic stimulation (rTMS) is a noninvasive neurostimulation technique that uses magnetic field to comprehensively influence events in the brain. Its use in patients after stroke focuses mainly on influencing brain neuroplasticity and therefore has the potential to improve motor functions in these patients. This study investigates the effect of rTMS on motor function recovery in patients in the acute stage of ischemic stroke.

Outliers of Treatment Frequency in Retinal Vein Occlusion: 24-Month Comparative Analysis of Fight Retinal Blindness! Practitioners.

Background: We aimed to describe a 2-year outcome of eyes managed by practitioners benchmarked using a funnel plot by their frequency of treatment using vascular endothelial growth factor (VEGF) inhibitors for naive retinal vein occlusion (RVO).

Methods: A multicentre, international, observational study of 29 doctors in 12 countries managing 1110 eyes with RVO commencing VEGF inhibitors between 1 January 2012-2022 tracked in the Fight Retinal Blindness! registry.

Results: We identified 3 outlying 'intensive' practitioners (managing 350/1110 eyes [32%]), 22 'typical' practitioners (604/1110, [54%]) and 4 outlying 'relaxed' practitioners (156/1110, [14%]) with respective 24-month outcomes in Branch and Central RVO including the primary outcome, mean adjusted change in visual acuity (VA) in BRVO: +16.