Label-Free Quantitative Phosphoproteomics in the Fission Yeast Schizosaccharomyces pombe.

Protein phosphorylation is a dynamic, reversible posttranslational modification that plays an important role in the regulation of cell signaling. Recently, label-free quantitative (LFQ) phosphoproteomics has become a powerful tool to analyze the phosphorylation of proteins within complex samples. In this chapter, we describe how to apply LFQ phosphoproteomics that is based on Fe-IMAC phosphopeptide enrichment followed by strong anion exchange (SAX) and porous graphitic carbon (PGC) fractionation strategies for identification and quantification of changes in the phosphoproteome in the fission yeast Schizosaccharomyces pombe.

Expression of individual members of the TGF-β/SMAD signalling pathway in the progression and survival of patients with colorectal carcinoma.

Current knowledge of tumor biology offers many "targets" for therapeutic intervention. The molecular basis of many processes that play a role in the pathogenesis of colorectal cancer has been identified. One part of colorectal cancer clinical trials is focused on testing substances in a group of patients with tumors in which the TGF-β signalling pathway is hyperactivated.

pH-sensitive aminated chitosan/carboxymethyl cellulose/aminated graphene oxide coated composite microbeads for efficient encapsulation and sustained release of 5-fluorouracil.

The application of smart pH-sensitive carriers has become an ideal choice for administering drugs with desired release profiles. Although pH-sensitive microbeads offer distinct benefits for delivering anticancer drugs orally, they encounter drawbacks, including low encapsulation efficiency, weak mechanical stability, biocompatibility concerns, and the risk of abrupt release. This study focuses on developing pH-sensitive coated composite microbeads for effective encapsulation and sustained release of 5-fluorouracil (5-FU).

Gyrophoric Acid, a Secondary Metabolite of Lichens, Exhibits Antidepressant and Anxiolytic Activity In Vivo in Wistar Rats.

Gyrophoric acid (GA) is a secondary metabolite of various lichens. It exhibits various biological activities in vitro, but only one study has been carried out in vivo. Because our previous study showed that GA stimulates neurogenesis in healthy rats, the current study aimed to explore the potential of GA during stress-induced depressive-like states in male Wistar rats.

Assessment of Bacterial Contamination and Antimicrobial Resistance of Isolates from Slovak Dairy Farms.

The conditions in livestock housing are suitable for the survival of airborne microorganisms, mainly due to high temperatures, humidity, and the presence of organic material. The total count of airborne bacteria concentrations in cattle farms ranged from 3.01 log CFU/mL to 6.

PETG as an Alternative Material for the Production of Drone Spare Parts.

Material selection is the main challenge in the drone industry. In this study, hardness, abrasive wear, impact resistance, tensile strength, and durability (frost resistance and accelerated ageing) were identified as important characteristics of drone materials. The additive manufacturing technology was used to produce the drone leg specimens and prototype.

The Role of HE4 in the Follow-Up of Advanced Ovarian, Fallopian Tube, and Primary Peritoneal Cancer-CEEGOG OX-01 Study.

: Ovarian, fallopian tube, and primary peritoneal cancers often share clinical characteristics and are typically diagnosed at advanced stages due to nonspecific symptoms. The utility of tumor markers, particularly CA125 and HE4, in the diagnosis and follow-up of these cancers remains an area of active investigation. : The CEEGOG (Central and Eastern European Gynecologic Oncology Group) OX-01 study aimed to evaluate HE4's role alongside CA125 in follow-up for advanced-stage ovarian, fallopian tube, and primary peritoneal cancers.

Influence of Strain Rate on Barkhausen Noise in Trip Steel.

This paper deals with Barkhausen noise in Trip steel RAK 40/70+Z1000MBO subjected to uniaxial plastic straining under variable strain rates. Barkhausen noise is investigated especially with respect to microstructure alterations expressed in terms of phase composition and dislocation density. The effects of sample heating and the corresponding Taylor-Quinney coefficient are considered as well.

A Case Study on the Possibility of Extending the Service Life of the Demining Machine Belt.

The operational practice of the design of the Bozena 5 demining machine has shown that its belts are the critical component that fundamentally affects the functionality of the entire machine. This article is a practical continuation and extension of the previous research results from the point of view of materials (research of the uniaxial fatigue life in bending and torsion), calculation (creation of the necessary mathematical, analytical and numerical models for the research) and construction (i.e.

Pediatric kidney transplantation in Europe, a clinical snapshot pilot.

Background: Pediatric kidney transplantations are rarely performed, and there is limited knowledge about the diversity in current clinical practices across Europe. This study aims to explore the utility of clinical snapshot studies in identifying these disparities, establishing a foundation for future snapshot studies and standardization efforts.

Methods: A pilot clinical snapshot study was conducted, with invitations extended to all 109 pediatric kidney transplant centres in Europe.

A tool for the assessment of the risk drivers and public perception of WASH in South Africa.

Unlabelled: Temporal trend analysis of the Google-search volumes and terms related to water, sanitation and hygiene (WASH) in South Africa was performed using a computer plugin between January 2004 and June 2022. This study was conducted as WASH has played an important role in the containment of the recent coronavirus disease 2019 (COVID-19) pandemic, and it is also one of the most effective and easiest-to-deploy tools in decreasing risk from infectious diseases. For the WASH-related terms, the monthly search volumes ranged from the minimum average of 480 for pit latrines to the maximum of 30236 for diarrhea or diarrhoea for the studied period.

Exploring the black box of human reproduction: endometrial organoids and assembloids - generation, implantation modeling, and future clinical perspectives.

One of the critical processes in human reproduction that is still poorly understood is implantation. The implantation of an early human embryo is considered a significant limitation of successful pregnancy. Therefore, researchers are trying to develop an ideal model of endometrium that can mimic the endometrial micro-environment as much as possible.

TLR4-mediated chronic neuroinflammation has no effect on tangle pathology in a tauopathy mouse model.

Introduction: Alzheimer's disease (AD) is marked by the accumulation of fibrillary aggregates composed of pathological tau protein. Although neuroinflammation is frequently observed in conjunction with tau pathology, current preclinical evidence does not sufficiently establish a direct causal role in tau tangle formation. This study aimed to evaluate whether chronic Toll-like receptor 4 (TLR4) stimulation, induced by a high dose of lipopolysaccharide (LPS, 5 mg/kg), exacerbates neurofibrillary tangle (NFT) pathology in a transgenic mouse model of tauopathy that expresses human truncated 151-391/3R tau, an early feature of sporadic AD.

Biogenic and risk elements in wild boar testes and relation to spermatozoa motility.

Environmental pollution results in serious health hazards to animals, reflected in biogenic and risk element concentrations in animal tissues. The objective of this study was to examinate concentration of selected elements in testes, and epididymal spermatozoa motility of wild boars. Wild boars were hunted in region Žuhračka - Levice, Slovak Republic.

The first record of Culicoides deltus as a putative vector of Onchocerca flexuosa in Slovak red deer (Cervus elaphus).

Within the research conducted in the years 2016-2022 in the area of Volovsky Mountains in Slovakia, 63,950 biting midges were collected during 74 trapping sessions. The aim of the study was to identify species composition of biting midges, their host preference and potential transmission of parasites by these insects under natural conditions. The collected biting midges fell into 29 species and the most common were the Culicoides (C.

PangeBlocks: customized construction of pangenome graphs via maximal blocks.

Background: The construction of a pangenome graph is a fundamental task in pangenomics. A natural theoretical question is how to formalize the computational problem of building an optimal pangenome graph, making explicit the underlying optimization criterion and the set of feasible solutions. Current approaches build a pangenome graph with some heuristics, without assuming some explicit optimization criteria.

Adapting generalized suitability curves from Brown trout to Minnow using 1D and 2D aquatic habitat models.

The article focuses on adapting generalized depth preference and flow velocity characteristics from Brown trout (Salmo trutta morpha fario) to the Minnow (Phoxinus phoxinus). The results obtained were used to model habitat suitability with 1D and 2D models. Since 1995, research on assessing aquatic habitat quality has been ongoing on 77 mountain streams in Slovakia.

Nano selenium in broiler feeding: physiological roles and nutritional effects.

Using nanotechnology, while improving the health of broiler chickens, it is possible to control and reduce the conflict of minerals in the intestines, and toxicity of and pollution by these elements. It could be shown that the antioxidant and immune modulation effects of nano selenium are significantly superior compared to other sources of selenium. In addition, improving the quality of meat products with the use of nano selenium has promising results in the future perspective of quality improvement and food safety.

The SQ tree sublingual immunotherapy tablet is effective and well tolerated in children-A pivotal phase III trial.

Background: Allergic rhinitis and/or conjunctivitis (AR/C) induced by tree pollen is common and negatively impacts quality of life in children and adolescents. This phase III trial investigated the efficacy and safety of the SQ tree SLIT-tablet in a paediatric population (5-17 years) with moderate-to-severe AR/C induced by pollen from birch and trees in the birch homologous group.

Methods: Nine hundred and fifty-two subjects were randomized (1:1) to daily treatment with SQ tree SLIT-tablet or placebo for up to 52 weeks and had free access to AR/C symptom-relieving medications.