Quantifying the Impact of High-Pressure Processing on the Phenolic Profile, Antioxidant Activity, and Pollen Morphology in Honey.

Honey can benefit from non-thermal processing techniques such as high-pressure processing (HPP) to improve its quality and bioactivity. This study investigated the impact of HPP (600 MPa for 5, 10, and 15 min) on honey's quality, including the levels of hydroxymethylfurfural (HMF), antioxidant activity, total phenolic content (TPC), and phenolic profile. HPP treatment did not significantly affect HMF or TPC levels but led to selective changes in the phenolic profile.

Role of exciton delocalization in chiroptical properties of benzothiadiazole carbon nanohoops.

Development of chiral organic materials with a strong chiroptical response is crucial to advance technologies based on circularly polarized luminescence, enantioselective sensing, or unique optical signatures in anti-counterfeiting. The progress in the field is hampered by the lack of structure-property relationships that would help designing new chiral molecules. Here, we address this challenge by synthesis and investigation of two chiral macrocycles that integrate in their structure a pseudo-meta [2.

Applications of artificial intelligence in regenerative dentistry: promoting stem cell therapy and the scaffold development.

Tissue repair represents a critical concern within the domain of dentistry. On a daily basis, countless individuals seek dental clinic services due to inadequate dental care. Many of the treatments that patients receive have unfavorable side effects.

A high-efficient electrochemical degradation of diclofenac in water on planar and microstructured 2D, and macroporous 3D boron-doped diamond electrodes: Identification of degradation and transformation products.

The highly efficient degradation of persistent organic substances by electrochemical advanced oxidation processes (EAOPs), which don't result in the formation of potentially harmful by-products, is crucial for the future of water management. In this study, boron-doped diamond electrodes (BDDE) with three morphologies (planar 2D, microstructured 2D, and macroporous 3D) were employed for the anodic oxidation of diclofenac (DCF) in two working electrolytes (NaCl and NaSO). In total, 11 by-products formed during the electrochemical oxidation of DCF were identified via HPLC-HRMS.

Advancing molecular diagnostics of myotonic dystrophy type 1 using short-read whole genome sequencing.

Myotonic dystrophy type 1 (DM1) is a serious multisystem disorder caused by GCA repeat expansions in the DMPK gene. Early and accurate diagnosis, often requiring reliable DNA-diagnostic techniques, is critical for preventing life-threatening cardiac complications. Clinically, two main diagnostic challenges exist.

Interaction of zinc oxide nanoparticles with soil colloidal suspensions.

The properties of soil colloids determine the interaction with nanoparticles, their behavior, and destiny in the soil environment including soil solutions. This study examines how several properties of soil colloids, including pH, phosphorus content, clay minerals, and iron oxyhydroxides, influence the interaction with zinc oxide nanoparticles (ZnO-nps). For the experimental setup, four different soils were selected from the temperate climate of central Europe, in Slovakia, exhibiting pH values ranging from 4.

Effect of multispecies swards on ruminal fermentation, methane emission and potential for climate care cattle farming - an in vitro study.

Greenhouse gas (GHG) emissions from livestock ruminants, particularly methane (CH), nitrous oxide, and indirectly ammonia (NH) significantly contribute to climate change and global warming. Conventional monoculture swards for cattle feeding, such as perennial ryegrass or Italian ryegrass, usually require substantial fertiliser inputs. Such management elevates soil mineral nitrogen levels, resulting in GHG emissions and potential water contamination.

Multiple regional outbreaks caused by global and local VIM-producing Klebsiella pneumoniae clones in Poland, 2006-2019.

Purpose: This study was aimed at comprehensive genomic analysis of VIM-type carbapenemase-producing Klebsiella pneumoniae species complex (KpSC) in Poland.

Methods: All non-duplicate 214 VIM-producing KpSC isolates reported in Poland in 2006-2019 were short-read sequenced and re-identified by the average nucleotide identity scoring. Their clonality/phylogeny was assessed by cgMLST and SNP in comparison with genomes from international databases.

Are We Estimating the Mean and Variance Correctly in the Presence of Observations Outside of Measurable Range?

Laboratory measurements used for safety assessments in clinical trials are subject to the limits of the used laboratory equipment. These limits determine the range of values which the equipment can accurately measure. When observations fall outside the measurable range, this creates a problem in estimating parameters of the normal distribution.

In vivo evaluation of selenium-tellurium based nanoparticles as a novel treatment for bovine mastitis.

Background: Bovine mastitis is one of the main causes of reduced production in dairy cows. The infection of the mammary gland is mainly caused by the bacterium Staphylococcus aureus, whose resistant strains make the treatment of mastitis with conventional antibiotics very difficult and result in high losses. Therefore, it is important to develop novel therapeutic agents to overcome the resistance of mastitis-causing strains.

The chemotactic response of Caenorhabditis elegans represents a promising tool for the early detection of cancer.

The nematode Caenorhabditis elegans, with its highly sensitive olfactory system, has emerged as a promising tool for testing chemotaxis. In the field of cancer diagnostics, there is a growing interest in the development of non-invasive screening methods for the detection of volatile organic compounds in a patient's urine. The objective of this study was to contribute to the existing body of knowledge by evaluating the ability of a Caenorhabditis elegans-based chemotaxis assay to discriminate between urine samples from healthy individuals and patients diagnosed with breast or colon cancer.

Global impact of ten-valent and 13-valent pneumococcal conjugate vaccines on invasive pneumococcal disease in all ages (the PSERENADE project): a global surveillance analysis.

Background: Pneumococcal conjugate vaccines (PCVs) that are ten-valent (PCV10) and 13-valent (PCV13) became available in 2010. We evaluated their global impact on invasive pneumococcal disease (IPD) incidence in all ages.

Methods: Serotype-specific IPD cases and population denominators were obtained directly from surveillance sites using PCV10 or PCV13 in their national immunisation programmes and with a primary series uptake of at least 50%.

Length-mass relationships of pond macroinvertebrates do not hold between Southern and Northern Europe.

The lack of reliable data on length-mass relationships, essential to obtain accurate biomass estimates, limits our ability to easily assess secondary production by aquatic invertebrates. In the absence of published equations from similar habitat conditions, authors often borrow equations developed in geographic regions with different climate conditions, which may bias biomass estimates. A literature overview of published size-mass relationships for Portugal and Sweden highlights the need for further data within these biogeographic regions.

The development of the Compassion Satisfaction and Compassion Fatigue scale.

Background: There is a high probability of compassion fatigue occurring in helping professionals who work with traumatized clients or patients. Several instruments exist for measuring compassion fatigue, but all of them have methodological flaws. The original Compassion Satisfaction/Fatigue Self-Test for Helpers is time-consuming and its psychometric properties, including factor structure, have not been supported in the research.

Moth flies (Diptera, Psychodidae) of Estonia.

A fundamental prerequisite for understanding and protecting biodiversity is the construction of a high-quality faunal database. The primary objective of this study was to address knowledge gaps in the biodiversity of the family Psychodidae in Estonia. Faunistic data on 45 species of moth flies (Diptera: Psychodidae) from Estonia are presented, including 30 new country-records.

Analysis of the 10-day ultra-marathon using a predictive XG boost model.

Objective: Ultra-marathon running races are held as distance-limited or time-limited events, ranging from 6 h to 10 days. Only a few runners compete in 10-day events, and so far, we have little knowledge about the athletes' origins, performance, and event characteristics. The aim of the present study was to investigate the origin and performance of these runners and the fastest race locations.

Extracellular vesicles from platelet-poor plasma possess anti-inflammatory and anti-catabolic effects in chondrocytes stimulated with IL-1β or synovial membrane-conditioned media.

Background: Although osteoarthritis (OA) is the most prevalent form of arthritis, there is still no effective treatment capable of combining immunomodulatory effects with cartilage repair. Extracellular vesicles (EVs) represent a promising new generation of cell-free therapies for OA. Blood-derived products, including plasma, are an easily available and abundant source of EVs with anti-inflammatory and regenerative properties.

Longitudinal Assessment of Cognitive Function in Survivors of Testicular Germ Cell Tumor.

Background And Objective: Survivors of testicular germ cell tumor (TGCT) may experience long-term cognitive changes. The aim of our prospective study was to longitudinally assess cognitive function among TGCT survivors to identify potential lasting cognitive changes over a period of 5 yr.

Methods: TGCT survivors (n = 151) completed Functional Assessment of Cancer Therapy-Cognitive Function (FACT-Cog) questionnaires annually, with median time to first follow-up visit (FUV) of 8 (range 4-24) yr since completion of treatment.

ECHINOCOCCUS MULTILOCULARIS IN NEW YORK WILDLIFE: DISTRIBUTION AND GENETIC DIVERSITY OF AN EMERGING PATHOGEN.

Echinococcus is a genus of cestode parasites of paramount veterinary and medical importance globally. Two species, Echinococcus granulosus sensu lato and Echinococcus multilocularis, are endemic to North America and are the etiologic agents of cystic echinococcosis and alveolar echinococcosis, respectively. North America is currently experiencing an epidemiological shift in the state of transmission, distribution, and prevalence of E.

Temperature-dependent sleep patterns in Drosophila.

Sleep is a fundamental physiological process conserved through evolution, from worms to humans. Understanding how temperature influences sleep is essential for comprehending the complexities of animal behavior, physiology, and their adaptations to thermal environments. This study explores the impact of temperature on sleep behavior and patterns in Drosophila melanogaster.

As, Cd, Hg, and Pb Biological Concentrations and Anthropometry in Slovak Adolescents.

Anthropometry provides a non-invasive technique for evaluating growth and obesity and serves as an indicator of health status. This cross-sectional study aims to investigate the association of internal arsenic (As), cadmium (Cd), total mercury (THg), methylmercury (MeHg), and lead (Pb) exposure with anthropometric parameters, including obesity, in adolescents. Participants (N = 320) were children aged 10-14 years (mean 11.

Who agrees more? The role of age, education, and the ability to solve verbal analogies in acquiescence.

Background: Acquiescence as one of the response styles is the participant's tendency to shift answers to agreement rather than to disagreement regardless of the items' content. Acquiescence together with other response styles could be a serious threat to the results of research. It can be affected by several individual characteristics including cognitive abilities.

Safety and Efficacy of BioMime Sirolimus-Eluting Stent System in All-Comers Real-World Population With Coronary Artery Stenosis: MILES Global Registry.

Background: This study evaluated the safety and efficacy of BioMime sirolimus-eluting stent (SES) system, with an ultra-low strut thickness (65 µm), in real-world all-comers population with coronary artery stenosis (CAD).

Methods: This was a post-marketing, multicenter, single-arm, observational clinical registry among patients undergoing intervention for CAD. Patients were clinically followed up at 1, 9, 12, and 24 months after the index percutaneous coronary intervention.