3,573 results match your criteria: "Skin Tissue Expansion"

Comprehensive analysis of skin growth-related hub genes and microenvironment characterization in a mouse expanded skin model.

Front Immunol

December 2024

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.

Background: Mechanical stretch-mediated tissue expansion is effective for obtaining extra skin and soft tissue required for the repair of defects or reconstruction of surface organs. Understanding the cellular and molecular mechanisms and identifying hub genes and key cell types associated with skin expansion could help predict the success of skin growth during expansion procedures.

Methods: We analyzed murine chip sequencing data and single-cell sequencing data available from the Gene Expression Omnibus database.

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Enhancing Aesthetics in Bilateral DIEP Flap Breast Reconstruction: the Role of Tissue Pre-Expansion.

Aesthetic Plast Surg

December 2024

Department of Plastic and Aesthetic Surgery, Sana Krankenhaus Gerresheim, Gräulinger Straße 120, D-40625, Düsseldorf, Germany.

Background: Achieving symmetrical outcomes in bilateral autologous breast reconstruction is challenging, particularly in cases of asymmetrical recipient sites. Tissue pre-expansion is proposed to improve aesthetics by enlarging the skin envelope for refined breast shaping. This study examines its efficacy in bilateral DIEP flap reconstructions.

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Background: Chronic erythroderma is a potentially life-threatening condition that can be caused by a variety of diseases, but approximately 30% of cases remain idiopathic, often with insufficient treatment options.

Objective: To establish a molecular disease map of chronic idiopathic erythroderma.

Methods: We performed single-cell RNA sequencing combined with T-cell receptor sequencing of blood and skin from 5 chronic idiopathic erythroderma (CIE) patients and compared results with 8 cases of erythrodermic cutaneous T-cell lymphoma (eCTCL), 15 moderate-to-severe atopic dermatitis (AD), 10 psoriasis and 20 healthy control (HC) individuals.

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Background: Adipose tissue provides an abundant source of stromal vascular fraction (SVF) cells for immediate administration. It can also give rise to many multipotent adipose-derived stromal cells. SVF is the population of cells obtained from mechanical or enzymatic digestion of lipoaspirate with no necessity for cell culture or expansion.

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Using a 6-week porcine full-thickness excisional wound grafting model, we evaluated the Autologous Regeneration of Tissue (ART®) System, a novel skin harvesting device designed to collect autologous full-thickness autologous microcolumns (FTAM) at 0.5 mm in diameter. The donor skin sites were harvested using the ART® System and compared to split-thickness skin grafts (STSGs).

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Evidence-based cesarean delivery: intraoperative management from skin incision until placental delivery (Part 8).

Am J Obstet Gynecol MFM

December 2024

Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Thomas Jefferson University, Philadelphia, PA. Electronic address:

The goal of standardizing the technique of the routine, uncomplicated cesarean delivery (CD) is to decrease maternal morbidity while optimizing neonatal outcomes. During the procedure, a family-oriented CD is recommended. The low transverse cesarean skin incision (created with either scalpel or diathermy) is preferred with either the Joel-Cohen or Pfannenstiel methods being acceptable.

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Background: Deep burns to the upper abdomen that involve the submammary fold can lead to downward contracture and may cause significant asymmetry in a single breast, which can cause serious aesthetic and psychological problems, especially when they occur during childhood or before puberty. The objective of this study was to establish a practical treatment approach for postburn unilateral breast reconstruction after downward contracture.

Method: We conducted this retrospective review of patients with postburn unilateral breast deformity who underwent reconstruction using the expanded lateral thoracic flap and torso advancement flap from 2016 through 2021.

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Precisely controlled remodeling of the basement membrane (BM) is crucial for morphogenesis, but its molecular and tissue-level dynamics, underlying mechanisms, and functional significance in mammals remain largely unknown due to limited visualization tools. We developed mouse lines in which the endogenous collagen IV gene (Col4a2) was fused with a fluorescent tag. Through live imaging of developing hair follicles, we reveal a spatial gradient in the turnover rate of COL4A2 that is closely coupled with both the BM expansion rate and the proliferation rate of epithelial progenitors.

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Synthesis, characterization, and application potential of chitosan/acrylamide composite hydrogels as skin expanders.

J Mater Sci Mater Med

November 2024

Department of Plastic Reconstructive Surgery & Hand Microsurgery, Ningbo NO.6 Hospital, 1059 East Zhongshan Road, Ningbo, China.

Hydrogels are currently widely used in regenerative medicine and wound repair due to their superior biocompatibility, reliable mechanical strength, and good morphological memory. We aimed to prepare a self-expanding hydrogel that can be used as a skin expander for the repair of large soft skin tissue defects. Self-expanding hydrogels were prepared by chemical cross-linking, which consisted of water-soluble chitosan (CS), acrylamide (AM), methylene bisacrylamide (NMBA), etc.

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Effects of Resveratrol on Adipocytes: Evidence from In Vitro and In Vivo Studies.

Molecules

November 2024

Department of Health Sciences, Brock University, St. Catharines, ON L2S 3A1, Canada.

Obesity, a prevalent global health issue, arises from an imbalance between caloric intake and energy expenditure, leading to the expansion of adipose tissue and metabolic dysfunction. White adipose tissue (WAT) stores energy as lipids, while brown adipose tissue (BAT) plays a pivotal role in energy dissipation through adaptive thermogenesis. Recent research initiatives have focused on finding strategies to decrease adipogenesis and fat mass accumulation and increase thermogenesis.

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Expansion limits of meshed split-thickness skin grafts.

Acta Biomater

January 2025

NSF Science and Technology Center for Engineering Mechanobiology, USA; Department of Mechanical Engineering, New Jersey Institute of Technology, USA. Electronic address:

Split-thickness skin grafts are widely used to treat chronic wounds. Procedure design requires surgeons to predict how much a patch of the patient's own skin expands when it is meshed with rows of slits and stretched over a larger wound area. Accurate prediction of graft expansion remains a challenge, with current models overestimating the actual expansion, leading to suboptimal outcomes.

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Article Synopsis
  • Hereditary hemochromatosis (HH) is a genetic condition that causes excessive iron buildup in various organs, leading to damage and health issues, while celiac disease (CD) is an autoimmune disorder that often results in iron deficiency.
  • A unique case of a young woman with both conditions highlights the challenges of managing iron levels, especially during pregnancy and after childbirth, due to increased iron needs.
  • The case underscores the necessity for comprehensive patient care, education, and monitoring by healthcare teams to avoid complications in women of child-bearing age.
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Subcutaneous delivery of mesenchymal stromal cells induces immunoregulatory effects in the lymph node prior to their apoptosis.

Stem Cell Res Ther

November 2024

Department of Anatomy and Developmental Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, 3800, Australia.

Background: Mesenchymal stromal cell (MSC) therapy commonly involves systemic infusion of MSCs, which undergo apoptosis in the lung and induce immunoregulatory macrophages that reduce disease. The relevance of this mode of action, however, is yet to be determined for MSCs administered via other routes. Here, we administered MSCs via subcutaneous (SC) injection into inflamed tissue and investigated the immunomodulatory effects on the local lymph node (LN), which is a major site for the initiation and regulation of immune responses.

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Article Synopsis
  • * It highlights various techniques like the modified latissimus dorsi musculocutaneous flap and the lateral intercostal artery perforator (LICAP) flap, demonstrating effective results and high patient satisfaction.
  • * The text also mentions the application of different flaps for specific situations such as oncologic resections, weight loss, and implant removal, emphasizing the advantages of utilizing nearby tissues for reconstruction.
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[Sincipital meningoencephaloceles: Clinical and surgical considerations].

Ann Chir Plast Esthet

November 2024

Service de chirurgie maxillo-faciale et plastique de la face, hôpital Purpan, CHU de Toulouse, place du Dr-Baylac, TSA 40031, 31059 Toulouse cedex 9, France.

Article Synopsis
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sequence type (ST) 88, encompassing both methicillin-resistant (MRSA) and methicillin-sensitive (MSSA) phenotypes, is globally prevalent and commonly associated with skin and soft tissue infections. Despite its widespread occurrence, comprehensive genomic studies on this clone remain scarce. In this study, we performed detailed genomic analyses on 130 ST88 isolates derived from severe bloodstream infections alongside 275 publicly available ST88 sequences.

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Article Synopsis
  • The study examines the effectiveness of different sizes of forehead tissue expanders in improving reconstructive outcomes and minimizing donor site deformities following surgery.
  • It analyzed data from 79 patients who received either small (50/80 mL) or large (150/200 mL) expanders, finding no major demographic differences between the two groups.
  • Results indicated that larger expanders led to less frontal deformation and better overall reconstruction results, suggesting that larger expanders are preferable for forehead procedures.
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MAIT cells: Conserved watchers on the wall.

J Exp Med

January 2025

INSERM ERL1305, CNRS UMR6290, Institut de Génétique and Développement de Rennes, Université de Rennes, Rennes, France.

MAIT cells are innate-like T cells residing in barrier tissues such as the lung, skin, and intestine. Both the semi-invariant T cell receptor of MAIT cells and the restricting element MR1 are deeply conserved across mammals, indicating non-redundant functions linked to antigenic specificity. MAIT cells across species concomitantly express cytotoxicity and tissue-repair genes, suggesting versatile functions.

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Being thin-skinned can still reduce damage from dynamic puncture.

J R Soc Interface

October 2024

Department of Evolution, Ecology, and Behavior, School of Integrative Biology, University of Illinois Urbana-Champaign, 505 S. Goodwin Avenue, Urbana, IL 61801, USA.

The integumentary system in animals serves as an important line of defence against physiological and mechanical external forces. Over time, integuments have evolved layered structures (scales, cuticle and skin) with high toughness and strength to resist damage and prevent wound expansion. While previous studies have examined their defensive performance under low-rate conditions, the failure response and damage resistance of these thin layers under dynamic biological puncture remain underexplored.

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Article Synopsis
  • - The research focuses on developing an ion-conductive silk fibroin (SF) hydrogel that combines glycidyl methacrylate SF (SilMA) with bioionic liquid choline acylate (ChoA) and modified functional groups for improved properties in medical and bioengineering applications.
  • - The new hydrogel shows impressive characteristics like strong adhesion, mechanical flexibility, and the ability to adapt to micro-physiological environments, making it suitable for use in tissue engineering and wearable electronic devices.
  • - The combination of ionic conductivity and biocompatibility in this SF hydrogel suggests significant potential for use in personalized healthcare applications, such as flexible skin sensors and other electronic devices.
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  • A CGG repeat expansion in the 5' UTR of the GIPC1 gene is linked to oculopharyngodistal myopathy (OPDM), a hereditary muscle disorder causing symptoms like eyelid drooping and muscle weakness.
  • This mutation has also been associated with Parkinson's disease, although without myopathy symptoms in those cases.
  • In two unrelated cases, patients with myopathic symptoms later developed parkinsonism, indicating that GIPC1-related repeat expansions may lead to various neuromuscular issues, highlighting the need to recognize central nervous system symptoms in OPDM2.
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Article Synopsis
  • * A total of 18 patients underwent the procedure, involving tissue expansion followed by tumor resection and flap reconstruction; most patients had successful recoveries with minimal complications.
  • * Follow-up results indicated no tumor recurrence, and satisfactory healing at both the lesion and donor sites, making this method beneficial for maintaining aesthetic outcomes post-surgery.
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Article Synopsis
  • Primary atopic disorders (PAD) are rare genetic conditions caused by specific gene variants that affect skin and immune function, making diagnosis challenging among common allergic disease cases.
  • Identifying PAD requires recognizing clinical red flags like family history and unusual infections, as conventional lab tests are inadequate for definitive diagnosis.
  • Whole-genome sequencing (WGS) enhances diagnostic efficiency and accuracy, but requires careful interpretation and collaboration among specialists to effectively manage PAD cases.
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Numerical investigation of new rete ridge formation in a multi-layer model of skin subjected to tissue expansion.

J Biomech

November 2024

School of Mechanical Engineering, Purdue University, West Lafayette, 47907, IN, USA; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, 47907, IN, USA. Electronic address:

The skin is a multilayered organ with microstructural and antomical heterogeneities that contribute to its unique mechanophysiology. Between the epidermis layer at the top and the dermis layer below, the basal keratinocytes form an interface with sinusoidal-like geometry termed rete ridges. In previous computational work we showed that the rete ridges contribute to lower delamination risk by increasing surface area and reducing the stress jump across the interface.

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