Novel Nanoliposomes Synergistically Modulated by Sitogluside and Dioscin: Stability, Bioavailability, and Capacity To Alleviate Hyperuricaemia.

Cholesterol (Cho) is commonly used to stabilize nanoliposomes; however, there is controversy on the relationship between Cho and health. In this study, we developed a novel multifunctional nanoliposome utilizing structurally similar sitogluside (SG) and dioscin (Dio) instead of Cho to anchor the phospholipid bilayer and synergistically modulate the membrane properties of the nanoliposome (DPPC or DOPC). The storage and gastrointestinal tract stability experiment demonstrated that the changes of physical and chemical properties, including the significantly reduced size and Dio retention rate of nanoliposomes synergistically modulated by SG and Dio compared to those of SG alone, regulated nanoliposomes.

Malaria elimination challenges in countries approaching the last mile: a discussion among regional stakeholders.

Background: The national malaria control programmes in Cambodia, Nepal, and Bhutan aim to achieve malaria elimination by 2025-2030. While the vivax malaria burden remains challenging, the consistent decline in falciparum malaria in these countries over the last five years suggests that the goal is achievable. However, unexpected cases in previously falciparum malaria-free districts continue to occur.

Effectiveness of Digital-Based Interventions on Physical and Psychological Outcomes Among Cancer Patients: A Systematic Review and Meta-Analysis.

Objective: Evolving digital technology has paved the way for endless potentiality. Leveraging on digital technology for healthcare purposes can target cancer patients, thus improving physical and psychological symptoms. Nevertheless, there is limited consolidated evidence on the effectiveness of virtual reality (VR) and mobile applications.

Development of Machine-learning Model to Predict Anticoagulant Use and Type in Geriatric Traumatic Brain Injury Using Coagulation Parameters.

This study aimed to investigate the patterns of anticoagulation therapy and coagulation parameters and to develop a prediction model to predict the type of anticoagulation therapy in geriatric patients with traumatic brain injury. A retrospective analysis was performed using the nationwide neurotrauma database of Japan. Elderly patients (≥65 years) with traumatic brain injury.

Factorial designs are accurate tools to pick up the most promising extremophiles for future biosurfactant production.

In this study, five strains previously isolated from black liquor (BL) and vinasse (V) were tested to assess the most promising regarding its capacity of biosurfactant production. For that, four factorial designs of two factors at two levels (2) were run for each strain. Selected factors were the production time and the composition media, while the surface tension reduction and optical density were the responses variables.

Transparency, trustworthiness and usefulness in pre-eclampsia randomised controlled trials in the last three decades.

Objectives: Over the last decades, there has been a rising number of randomised controlled trials (RCTs) on pre-eclampsia. We investigated pre-eclampsia RCTs between 1987 and 2021 and reported on trustworthiness, risk of biases, p-values, transparency, and usefulness.

Methods: We searched PubMed for RCTs containing "pre-eclampsia" or "hypertensive disorders of pregnancy" in the title between 1987 and 2021.

Trust in government vaccine recommendations during the Covid-19 pandemic in Singapore: A longitudinal survey study.

Background: Trust in governments has been decreasing in recent years, especially during the Covid-19 pandemic, where low-trust societies showed reduced compliance with disease control measures. Few studies have examined how trust in authorities changed over the pandemic. This study investigated the trajectory of public trust in the Singapore government's vaccine recommendations during this period.

Advancements in glycan analysis: high performance anion exchange chromatography-pulsed amprometric detection coupled with mass spectrometry for structural elucidation.

Carbohydrates are essential biomolecules that play a vital role in various biological processes across humans, plants, and bacteria. Despite their ubiquity, the structural elucidation of carbohydrates, particularly oligo- and polysaccharides, remains a significant challenge due to their complex and heterogeneous nature. The high-performance anion exchange chromatography (HPAEC) or called ion chromatography (IC) coupled with pulsed amperometric detection (PAD) has emerged as a powerful tool for highly effective separation and highly specific detection of glycans.

Comprehensive industry-relevant black soldier fly bioconversion characterisation by a novel chamber system.

Black soldier fly larvae (BSFL) efficiently convert biowaste into valuable animal feed. Sustainable and reliable bioconversion is desirable to achieve optimal economic and environmental outcomes. Thus, science and industry require an accessible research platform to study complex bioconversion processes under conditions mirroring industrial-scale settings.

Assessing the impact of car-following driving style on traffic conflict risk using asymmetric behavior model and explainable machine learning.

To deepen the understanding of the impact of car-following driving style (CFDS) on traffic conflict risk and address the lack of clear CFDS evaluation metrics, this study proposes an improved CFDS metric based on the Asymmetric Behavior (AB) theory. Interpretable machine learning models were utilized for regression analysis to examine the relationship between CFDS and conflict risk. The generalized AB model calculates the difference between vehicle trajectories and the Newell trajectory, constructing the driving style evaluation metric, which quantifies driver aggressiveness in a manner that is both computationally straightforward and easily interpretable.

Self-maintenance of zonal hepatocytes during adult homeostasis and their complex plasticity upon distinct liver injuries.

Hepatocytes are organized into distinct zonal subsets across the liver lobule, yet their contributions to liver homeostasis and regeneration remain controversial. Here, we developed multiple genetic lineage-tracing mouse models to systematically address this. We found that the liver lobule can be divided into two major zonal and molecular hepatocyte populations marked by Cyp2e1 or Gls2.

Neurotransmitter-Mimicking Nanovesicles Facilitate Postoperative Glioblastoma Stem Cell-Specific Treatment for Preventing Tumor Recurrence.

Survival quality of glioblastoma (GBM) patients remains undesirable despite the aggressive multimodal treatment methods implemented, which are strongly associated with tumor recurrence after surgical resection. Self-renewal and strong tumourigenic capacity of glioblastoma stem cells (GSCs) at the narrow margin of the incision are essential factors driving tumor secondary strikes. Currently, the challenges in treating postoperative residual GSCs are mainly due to the lack of materials for incision and GSCs targeting.

Hydrolysis of palm kernel meal fibre using a newly isolated Bacillus subtilis F6 with high mannanase activity.

Palm kernel meal (PKM) presents a challenge for non-ruminant livestock feeding due to its high fibre content predominantly in the form of mannan. Microbial fermentation offers a sustainable solution for fibre hydrolysis in lignocellulosic biomass. In this study, a Bacillus subtilis strain (F6), with high mannanase secretion capability, was isolated from the environment.

Student perspectives on interdisciplinary learning in public health education: insights from a mixed-methods study.

Interdisciplinary learning is essential for equipping future health professionals to tackle the complexities of contemporary public health. This mixed-methods study investigates the experiences and perspectives of undergraduate public health students in Singapore on interdisciplinary learning in higher education and employed a convergent parallel design by combining a cross-sectional survey with in-depth interviews. Quantitative data were used to assess the relationship between students' exposure to interdisciplinary learning and its perceived value, while qualitative analysis explored key themes related to facilitators and barriers.

Risk for Flexor Pollicis Longus Rupture after Volar Lock Plating for Distal Radius Fracture Lasts Over a Decade.

Objectives: Volar locking plate (VLP) fixation is a very common procedure due to the high incidence of distal radius fractures (DRFs). Attritional flexor tendon rupture is a rare, but recognized complication after VLP fixation. There is no current consensus to prevent the condition.

A new approach in generating stable crack propagation at twisted bilayer graphene/hBN heterostructures.

Thermodynamic theory suggests that the obvious mechanical behavior caused by temperature and interlayer angle will affect the physical properties of materials, such as mechanical properties and transportation behavior, and it is different from the behavior in three-dimensional bulk materials. We observe an abnormal physical effect of bilayer graphene/hexagonal boron nitride (G/BN)-carbon nanotube (CNT) heterostructures, with a normalized out-of-plane deformation and normalized bond angle percentage to almost several times higher those of pristine G/BN heterostructures (without CNT) at 700-800 K. Our combined finite element theory and molecular dynamics simulations confirmed that the combination of CNT and interlayer angle diverted and bridged the propagating crack and provided a stable crack propagation path and crack tip opening displacement, resulting in the stress fields to be controlled around the CNT at high temperature.

Environmental Control of Queuosine Levels in Streptococcus mutans tRNAs.

Queuosine (Q) is a modification of the wobble base in tRNAs that decode NA(C/U) codons. It is ubiquitous in bacteria, including many pathogens. Streptococcus mutans is an early colonizer of dental plaque biofilm and a key player in dental caries.

Clinical features, risk factors, outcomes, and prediction model for intrahepatic and perihepatic abscess following hepatectomy for hepatocellular carcinoma.

Background: Intrahepatic and perihepatic abscess (IPHA) is a severe yet understudied complication that can occur after hepatectomy. This multicenter study aimed to elucidate the clinical features, risk factors, and outcomes of IPHA after hepatectomy for hepatocellular carcinoma (HCC), and to develop a novel prediction model for personalized risk assessment.

Methods: This was a multicenter cohort study of HCC patients who underwent curative-intent hepatectomy.

CRISPR-Cas spacer acquisition is a rare event in human gut microbiome.

Host-parasite relationships drive the evolution of both parties. In microbe-phage dynamics, CRISPR functions as an adaptive defense mechanism, updating immunity via spacer acquisition. Here, we investigated these interactions within the human gut microbiome, uncovering low frequencies of spacer acquisition at an average rate of one spacer every ∼2.

The environmental impact and sustainability of infection control practices: a systematic scoping review.

Infection prevention and control (IPC) programs form the basis of minimizing spread of pathogens in the healthcare setting and beyond. The COVID-19 pandemic amplified the demand for IPC. However, the environmental impact of IPC practices has yet to be addressed and attempts to quantify its climate implications have been sparse.

Associations between shorter leucocyte telomere length and increased risk of age-related macular degeneration in women: insights from the United Kingdom Biobank study.

Objectives: To determine the association between telomere length (TL) and age-related macular degeneration (AMD) and examine the potential variations with sex and ethnicity.

Methods: Population-based, cross-sectional study. A total of 52,083 participants from the UK Biobank were included.

A Systematic Review on the Effectiveness and Safety of Combining Biostimulators with Botulinum Toxin, Dermal Fillers, and Energy-Based Devices.

Introduction: Aesthetic medicine has evolved towards minimally invasive procedures, with biostimulators like Poly-L-Lactic Acid (PLLA), Calcium Hydroxylapatite (CaHA), and Polycaprolactone (PCL) gaining attention for their role in collagen induction, improving skin texture, elasticity, and volume. Combining these agents with other treatments-such as botulinum toxin, dermal fillers, and energy-based devices (e.g.

Genomic and transcriptomic profiling of radioresistant prostate and head and neck cancers implicate a BAHD1-dependent modification of DNA damage at the heterochromatin.

Radiotherapy is an integral modality in treating human cancers, but radioresistance remains a clinical challenge due to the involvement of multiple intrinsic cellular and extrinsic tumour microenvironment factors that govern radiosensitivity. To study the intrinsic factors that are associated with cancer radioresistance, we established 4 radioresistant prostate (22Rv1 and DU145) and head and neck cancer (FaDu and HK1) models by irradiating their wild-type parentals to 90 Gy, mimicking the fractionated radiotherapy schema that is often using in the clinic, and performed whole exome and transcriptome sequencing of the radioresistant and wild-type models. Comparative genomic analyses detected the enrichment of mismatch repair mutational signatures (SBS6, 14, 15, 20) across all the cell lines and several non-synonymous single nucleotide variants involved in pro-survival pathways.

Mechanical Circulatory Support for Massive Pulmonary Embolism.

Up to 50% of patients with pulmonary embolism (PE) experience hemodynamic instability and approximately 70% of patients who die of PE experience an accelerated cascade of symptoms within the first hours of onset of symptoms, thus necessitating rapid evaluation and intervention. Venoarterial extracorporeal membrane oxygenation and other ventricular assist devices, depending on the hemodynamic derangements present, may be used to stabilize patients with massive PE refractory to initial therapies or with contraindications to other interventions. Given the abnormalities in both pulmonary circulation and gas exchange caused by massive PE, venoarterial extracorporeal membrane oxygenation may be considered the preferred form of mechanical circulatory support for most patients.