2 results match your criteria: "Sichuan University School of Life Science[Affiliation]"
[Application of multicolor primed in situ labeling in simultaneous identification of chromosomes 18, X and Y in uncultured amniocytes].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
August 2009
Institute of Medical Genetics, Sichuan University School of Life Science, Key Laboratory of Bioresources and Eco-environment, Ministry of Education, Chengdu, Sichuan, 610064 P.R. China.
Objective: To establish a multicolor primed in situ labeling (PRINS) protocol for chromosome detection in uncultured amniocytes.
Methods: Chromosomes 18, X and Y in uncultured amniocytes were simultaneously detected by using the non-ddNTP-blocking multicolor PRINS procedure.
Results: Within 7 h, the 3 chromosomes were simultaneously marked in the same uncultured amniocyte.
[Detection of the SRY gene by primed in situ labeling].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
December 2008
Institute of Medical Genetics, Sichuan University School of Life Science, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, Chengdu, Sichuan 610064, P. R. China.
Objective: To establish a primed in situ labeling (PRINS) technique which can be more effective in detection of single copy gene.
Methods: On the basis of traditional PRINS, new reagents and procedures, such as TaqStart antibody, four primers of the sex determining region Y (SRY) gene and TSA(TM) Biotin System were included in detection of the SRY gene. Meanwhile, fluorescence in situ hybridization(FISH) to detect the SRY gene was used as control.