1 results match your criteria: "Shiraz Sciences University of Medical Sciences[Affiliation]"
A Single Tube Overlap Extension PCR Method for Splicing of Multiple DNA Fragments.
Avicenna J Med Biotechnol
January 2020
Diagnostic Laboratory Sciences and Technology Research Centre, Faculty of Paramedical, Shiraz Sciences University of Medical Sciences, Shiraz, Iran.
Background: Despite the ease of conventional splicing by overlap-extension (SOEing) PCR technique in theory, when splicing more than two fragments, and especially if one of the complementary sequences is A-T rich, the attachment of the fragments would be challenging. A new rapid and highly efficient SOEing PCR assay was developed for simultaneous splicing of multiple DNA fragments and induction of site-directed mutagenesis in a single tube.
Methods: The method was adapted for splicing human beta-globin UTRs to OCT4, SOX2, KLF4, C-MYC, LIN28A, and destabilized GFP for the construction of chimeric DNA fragments for transcription.