Comparison of different combinations of antibodies and labeled fluorescein in the detection of lymphocyte subsets by flow cytometry.

Flow cytometry is a classical method for analyzing human peripheral blood lymphocyte subsets. This study aims to explore a new combination of antibody and labeled fluorescein for detecting lymphocyte subsets by comparing the effects of different combinations of antibody and labeled fluorescein in flow cytometry. We conducted a prospective study and enrolled 362 healthy patients undergoing physical examination in the medical examination center of the third hospital of hebei medical university.

New method for global alignment of 2 DNA sequences by the tree data structure.

We introduce a new approach to investigate problem of DNA sequence alignment. The method consists of three parts: (i) simple alignment algorithm, (ii) extension algorithm for largest common substring, (iii) graphical simple alignment tree (GSA tree). The approach firstly obtains a graphical representation of scores of DNA sequences by the scoring equation R(0)*R-S(0)*S-T(0)*(a+bk).

Classification analysis of dual nucleotides using dimension reduction.

We introduce a new approach to investigate the dual nucleotides compositions of 11 Gram-positive and 12 Gram-negative eubacteria recently studied by Sorimachi and Okayasu. The approach firstly obtains a 16-dimension vector set of dual nucleotides by PN-curve from the complete genome of organism. Each vector of the set corresponds to a single gene of genome.

Numerical characterization of DNA sequences based on digital signal method.

Based on digital signal method, we propose a new representation of DNA primary sequence. The representation can completely avoid loss of information in the transfer of data from a DNA sequence to its mathematical representation. Afterwards, we suggest one such approach to reach quantification of similarities based on digital signal similarity theory.

New 3D graphical representation of DNA sequence based on dual nucleotides.

We introduce a 3D graphical representation of DNA sequences based on the pairs of dual nucleotides (DNs). Based on this representation, we consider some mathematical invariants and construct two 16-component vectors associated with these invariants. The vectors are used to characterize and compare the complete coding sequence part of beta globin gene of nine different species.