Cardiac-targeting transfection of tissue-type plasminogen activator gene to prevent the graft thrombosis and vascular anastomotic restenosis after coronary bypass.

Aim: To observe the tissue-type plasminogen activator gene (t-PA) plasmid packaged with albumin nanoparticles crosslinked to albumin ultrasound microbubbles for targeting transfection to myocardium to prevent the graft thrombosis and vascular anastomotic restenosis after coronary bypass.

Methods: A dog model of coronary bypass using the autoallergic saphenous vein as the graft was made. A highly expressive t-PA gene plasmid packaged with albumin nanoparticles crosslinked to albumin ultrasound microbubbles was constructed.

Ultrasound-targeted transfection of tissue-type plasminogen activator gene carried by albumin nanoparticles to dog myocardium to prevent thrombosis after heart mechanical valve replacement.

Background: There are more than 300,000 prosthetic heart valve replacements each year worldwide. These patients are faced with a higher risk of thromboembolic events after heart valve surgery and long-term or even life-long anticoagulative and antiplatelet therapies are necessary. Some severe complications such as hemorrhaging or rebound thrombosis can occur when the therapy ceases.

Preparation of ultrasound microbubbles crosslinked to albumin nanoparticles packaged with tissue-type plasminogen activator gene plasmid and method of in vivo transfection.

Aims: To observe the effect of constructed ultrasound microbubble crosslinked to albium nanoparticles packaged with tissue-type plasminogen activator (tPA) gene plasmid on the in vivo transfection.

Methods: The rabbits were chosen for all experiments. A highly expressive gene plasmid for tPA was constructed and packaged into a prepared nanoparticle with bovine serum albumin (BSA).

Study on construction of nano tPA plasmid to prevent thrombosis after mechanical valve replacement in dogs.

Background: Mechanical valve is inclined to induce thrombosis. We intend to elucidate the transfection of nano tissue-type plasminogen activator (tPA) gene plasmid to prevent thrombosis after tricuspid mechanical valve replacement in dogs.

Methods: A dog model of mechanical tricuspid valve replacement was constructed.

[Diagnostic performance of prospective versus retrospective electrocardiogram-gated 64-slice computed tomography coronary angiography].

Objective: To compare the diagnostic performance of prospective electrocardiogram (ECG)-triggered computed tomography coronary angiography (CTCA) versus retrospective ECG-gated CTCA.

Methods: Patients with suspected coronary artery disease divided into two groups which underwent 64-slice CTCA with prospective ECG-triggered or retrospective ECG-gated scanning (n = 100 each, HR < or = 65 bpm). Multi-planar reconstruction (MPR), curved-planar reconstruction (CPR), maximum intensity projection (MIP) and volume rendering (VR) were made to demonstrate the coronary arteries.

Brain protection by using innominate artery cannulation during aortic arch surgery.

The innominate artery cannulation (IAC) through the same sternotomy incision was used for 68 patients with aneurysm, involving the ascending aorta or the aorta arch. The IAC can get adequate flows during cooling and re-warming of cardiopulmonary bypass. It can also provide sufficient antegrade perfusion for brain during hypothermia circulation arrest.

[Changes in content of inflammatory cytokines and troponin I in peri-operative periods of coronary artery bypass grafting with or without cardiopulmonary bypass].

Objective: To evaluate the effects of coronary artery bypass grafting (CABG) with or without cardiopulmonary bypass (CPB) on inflammatory response and myocardial injury.

Methods: Sixty patients undergoing elective CABG were randomly divided into CABG with CPB group (CCABG, 30 cases) and CABG without CPB group (OPCABG, 30 cases). Blood samples were collected from central vein to assay interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-alpha (TNF-alpha), MB isoenzyme of creatine kinase (CK-MB) and troponin I (cTnI) before operation and 2, 8, 24, as well as 48 hours after operation, and all clinical data of the patients were recorded accordingly.

[Clinical significance of plasma free 15-F2t-isoprostane concentration during coronary artery bypasses graft surgery].

Objective: To analyse retrospectively the variation of plasma 15-F2t-isoprostane concentration during cardiac surgery and the relation with early myocardial dysfunction following normothemic cardiac surgery.

Methods: Thirty patients scheduled for coronary artery bypass graft surgery using normothermic cardiopulmonary bypass (CPB) and warm intermittent blood, crystalloid cardioplegia were enrolled. Patients were divided into two groups treated with (group II) or without (group I) positive inotropic drugs.