Characterization of the novel KIR3DL3*116 allele identified in a Chinese Han individual.

The novel KIR3DL3*116 allele differs from the closest allele KIR3DL3*00902 by a single missense mutation.

Identification of the novel KIR3DL3*118 allele in a Chinese Han individual.

The novel KIR3DL3*118 allele differs from the closest allele KIR3DL3*01002 by a single missense mutation at CDS nt502 A > G in exon 4.

The novel KIR3DL3*117 allele identified by sequencing-based typing in a Chinese Han individual.

The novel KIR3DL3*117 allele differs from the closest allele KIR3DL3*01002 by two mutations in exon 3.

[Using Next-Generation Sequencing Technology to Confirm the HLA Rare Alleles Detected by PCR-SSOP].

Objective: To confirm the HLA genotypes of the samples including 4 cases of magnetic bead probe HLA genotyping result pattern abnormality and 3 cases of ambiguous result detected by PCR sequence-specific oligonudeotide probe (SSOP) method.

Methods: All samples derived from HLA high-resolution typing laboratory were detected by PCR-SSOP. A total of 4 samples of magnetic bead probe HLA genotyping result pattern abnormality and 3 samples of ambiguous result were further confirmed by PCR sequence-based typing (SBT) technology and next-generation sequencing (NGS) technology.

Demographic Factors Among HIV Confirmed Blood Donors from 2013 to 2021 in Shenzhen.

Background: New HIV (Human immune deficiency virus) infections are continuously increasing in China and it remains a huge challenge to blood donation. As access to health services has affected by COVID-19 (Corona virus disease 2019) pandemic, a drop in new diagnoses (especially HIV) was observed worldwide.

Methods: During 2013-2021, 735,247 specimens from unpaid blood donors collected by Shenzhen Blood Center underwent ELISA (Enzyme -linked immunosorbent assay) and NAT (Nucleic acid test).

[The Activity Characteristics of ABO Blood Group Glycosyltransferases and the Effect of Plasma Preservation on its activity].

Objective: Activities of ABO blood group glycosyltransferases in the plasma of blood donors with different blood groups were detected to discover their normal ranges. In addition, the influence of different plasma storage temperatures and time on the enzyme activity was studied, so as to establish a stable ABO blood group glycosyltransferase activity detection technology system for the auxiliary identification of ABO blood groups.

Methods: Detect the activities of glycosyltransferase A (GTA) in plasma of type A, AB and O blood donors, and glycosyltransferase B (GTB) in plasma of type B, AB and O blood donors, respectively, to determine the activity range of GTA and GTB in the plasma of normal blood group under this detection technique.

Identification of the novel HLA-B*46:01:33 allele by next generation sequencing in a Chinese individual.

B*46:01:33 differs from B*46:01:01:01 by one nucleotide change at nucleotide 105 in exon 2 from C to T.

A novel HLA-C null allele, HLA-C*08:236N, identified by next-generation sequencing in a Chinese individual.

HLA-C*08:236N differs from C*08:01:01 by a single nucleotide exchange in exon 5 at position 1991.

Naturally occurring anti-PP1P in a Chinese individual with p phenotype: A case based on compound heterozygosity including one novel allele.

Background: The null phenotype in P1PK blood group, known as "p," is extremely rare in the whole world. Individuals of p phenotype spontaneously form anti-PP1P isoantibody. Here, we report a case of p phenotype with naturally occurring anti-PP1P isoantibodies in a Chinese individual.

Identification of the novel KIR3DL1*00703 allele in a Chinese Han individual.

The novel KIR3DL1*00703 allele differs from the closest allele KIR3DL1*00701 by a single silent mutation.

The novel KIR2DL4*00108 allele identified by sequencing-based typing in a Chinese Han individual.

The novel KIR2DL4*00108 allele differs from the closest allele KIR2DL4*00102 by a single synonymous mutation.

Characterization of the novel KIR3DL1*01507 allele identified in a Chinese Han individual.

The novel KIR3DL1*01507 allele differs from the closest allele KIR3DL1*01502 by a single synonymous mutation.

Full-length sequence of the novel HLA-C*03:04:74 allele by next generation sequencing in a Chinese individual.

C*03:04:74 differs from C*03:04:01:02 by one nucleotide change at nucleotide 1047 in exon 6 from G to A.

Full-length sequence of the novel HLA-C*03:566 allele by next-generation sequencing in a Chinese individual.

HLA-C*03:566 differs from HLA-C*03:04:01:02 by one nucleotide substitution at nucleotide 92 in exon 2 from A to G.

A novel HLA-DQB1*04 variant, HLA-DQB1*04:90, identified in a Chinese Han individual.

Identification of the novel HLA-DQB1*04:90 allele that differs from DQB1*04:01:01:01 at nucleotide 183 in exon 2 from A to T.

[Exclusion of HLA-C Genotype with Zero Mismatched PCR-SBT Results by Next Generation Sequencing].

Objective: Three cases of rare alleles of HLA-C with zero mismatched PCR-SBT results were analyzed by full-length sequencing to determine the true genotypes.

Methods: Three rare HLA-C alleles with zero mismatched PCR-SBT results were screened from clinical transplant matching samples, and the full-length sequence was detected by next-generation sequencing technology.

Results: The results of PCR-SBT typing of 3 samples were: HLA-C*03:04, 12:167; HLA-C*07:291, 15:02; HLA-C*01:43, 08:16.

HLA-DRB1*14:239, a novel HLA-DRB1 allele with one exonic mutation.

HLA-DRB1*14:239 differs from HLA-DRB1*14:03:01 by one nucleotide substitution in codon 82 in exon 2.

[Establishment of sequence-based typing assay for KIR2DS4 gene and identification of a new allele KIR2DS4*016].

Objective: To establish a reliable sequence-based typing method for KIR2DS4 and study its allele polymorphism in Chinese Han population.

Methods: Using PCR-SSP method to detect the positive or negative of KIR2DS4 gene in 222 random Chinese Han individuals, and then using the method of high fidelity and long-fragment PCR-SBT to amplify, sequence and genotype the exons 4 and 5 of KIR2DS4 positive individuals.

Results: We successfully amplified the fragment with 3.

[Molecular polymorphism Analysis on CD36 Deficiency among Platelet Blood Donors in Shenzhen].

Objective: To analyze the molecular polymorphisms of CD36 among 58 blood donors with CD36 deficiency and compare with CD36 positive controls.

Methods: A total of 58 donors with CD36 deficiency during a screening conducted in the laboratory from September 2019 to December 2020 were enrolled as the test group, including 39 males and 19 females, while 120 platelet donors with CD36 positive were randomly selected as the controls, including 76 males and 44 females. All of the subjects were Han nationality.

CBX4 contributes to HIV-1 latency by forming phase-separated nuclear bodies and SUMOylating EZH2.

The retrovirus HIV-1 integrates into the host genome and establishes a latent viral reservoir that escapes immune surveillance. Molecular mechanisms of HIV-1 latency have been studied extensively to achieve a cure for the acquired immunodeficiency syndrome (AIDS). Latency-reversing agents (LRAs) have been developed to reactivate and eliminate the latent reservoir by the immune system.

High-Frequency Notable HBV Mutations Identified in Blood Donors With Occult Hepatitis B Infection From Heyuan City of Southern China.

Background: All Chinese blood centers have implemented mini pool (MP) HBV nucleic acid testing (NAT) together with HBsAg ELISA in routine donor screening since 2015. The prevalence of occult hepatitis B virus infection (OBI) in donors from different regions varies, and the molecular characterization of the HBV DNA and clinical outcomes of these OBIs remain largely unexplored.

Methods: Blood donations from Heyuan city in Southern China were screened by HBsAg ELISA and HBV MP8 NAT.

Discovery of the HLA-C*08:99 allele in a Chinese individual.

HLA-C*08:99 differs by one non-synonymous nucleotide from C*08:01:01 in exon 5, codon 288 GTT>ATT.

Donor with HLA-C2 is associated with acute rejection following liver transplantation in Southern Chinese.

Apart from presenting peptides to T cells, class I HLA molecules serve as ligands for killer cell immunoglobulin-like receptor (KIRs) and regulate the response of natural killer (NK) cells. The role played by HLA and KIR in the acute rejection (AR) following liver transplantation has been controversial. In this retrospective study, we assessed the influence of class I HLA alleles, HLA matching between donor-recipient pairs, recipient KIR and donor HLA ligands on AR following liver transplantation in southern Chinese.