Identification of the Novel HLA-DQA1*05:01:16 Allele by Next Generation Sequencing in a Chinese Individual.

HLA-DQA1*05:01:16 differs from DQA1*05:01:01:01 by two nucleotide substitutions, one in exon 4 and one in intron 1.

A novel null HLA-B allele, B*40:510N, due to a single nucleotide deletion in exon 2.

One nucleotide deletion in codon 15 of HLA-B*40:01:02:01 results in a novel null allele, HLA-B*40:510N.

The novel non-classical HLA-G*01:55 identified in a Chinese individual.

The non-classical HLA-G*01:55 allele differs from G*01:01:12 at one position in exon 4.

MICB*002:06, a novel exonic variant of MICB (MHC class I chain-related gene B).

MICB*002:06 differs from MICB*002:01:01 by one nucleotide change at nucleotide 33 in exon 1 from C to T.

A novel HLA-DRB4 allele, HLA-DRB4*01:179.

One nucleotide substitution in codon 30 of HLA-DRB4*01:03:01:01 results in a novel allele, HLA-DRB4*01:179.

Recognition of the HLA-DRB3*03:65 allele in a Chinese individual.

We report a novel HLA-DRB3*03 allele, now named DRB3*03:65, identified by next-generation sequencing.

Two novel HLA class II alleles, HLA-DRB1*09:57 and HLA-DRB1*09:58 in Chinese individuals.

Compared with HLA-DRB1*09:01:02:05, the alleles HLA-DRB1*09:57 and HLA-DRB1*09:58 each show one nucleotide change, respectively.

Full-length sequence of the novel allele, HLA-B*58:01:40, identified in a Chinese individual.

HLA-B*58:01:40 differs from HLA-B*58:01:01 by a single nucleotide change in exon 3, 507 C- > T (codon 145.3 CGC- > CGT).

[Establishment of a genotyping method for the junior blood group and identification of a rare blood type with partial DVI.3 and Jr(a-)].

Objective: To develop a genotyping method for the Junior blood type and report on a rare blood type with Jr(a-).

Methods: Healthy O-type RhD+ volunteer donors of the Shenzhen Blood Center from January to May 2021 (n = 1 568) and a pedigree with difficult cross-matching (n = 3) were selected as the study subjects. Serological methods were used for proband's blood type identification, unexpected antibody identification, and antibody titer determination.

Full-length sequence of the novel HLA-A*02:1103 allele by next generation sequencing in a Chinese individual.

HLA-A*02:1103 differs from HLA-A*02:01:01:01 by one nucleotide change at nucleotide 811 in exon 4 from G to A.

Next-generation HLA sequencing reveals the novel HLA-A*33:244 allele.

The novel HLA-A*33:244 allele contains a c.553G>A substitution in exon 3 compared with A*33:03:01:01.

The novel HLA-B*13:179 variant detected by next generation sequencing in a Chinese individual.

HLA-B*13:179 differs from HLA-B*13:99 by one nucleotide substitution at position 829(A>G) in exon 4.

A single nucleotide substitution in exon 5 produced the novel allele, HLA-B*40:01:83.

HLA-B*40:01:83, carrying a single nucleotide substitution in exon 5 is described.

Full-length sequence of the novel HLA-B*40:537 allele by next-generation sequencing in a Chinese individual.

One nucleotide substitution in codon 116 of HLA-B*40:06:01:12 results in a novel allele, HLA-B*40:537.

The novel HLA-B*15:664 allele, identified by next-generation sequencing in a Chinese individual.

B*15:664 differs from B*15:02:01:01 by one nucleotide change at nucleotide 755 in exon 4 from C to G.

Identification of the novel HLA-B*46:01:33 allele by next generation sequencing in a Chinese individual.

B*46:01:33 differs from B*46:01:01:01 by one nucleotide change at nucleotide 105 in exon 2 from C to T.

Naturally occurring anti-PP1P in a Chinese individual with p phenotype: A case based on compound heterozygosity including one novel allele.

Background: The null phenotype in P1PK blood group, known as "p," is extremely rare in the whole world. Individuals of p phenotype spontaneously form anti-PP1P isoantibody. Here, we report a case of p phenotype with naturally occurring anti-PP1P isoantibodies in a Chinese individual.

Full-length sequence of the novel HLA-C*03:04:74 allele by next generation sequencing in a Chinese individual.

C*03:04:74 differs from C*03:04:01:02 by one nucleotide change at nucleotide 1047 in exon 6 from G to A.

Full-length sequence of the novel HLA-C*03:566 allele by next-generation sequencing in a Chinese individual.

HLA-C*03:566 differs from HLA-C*03:04:01:02 by one nucleotide substitution at nucleotide 92 in exon 2 from A to G.

A novel HLA-DQB1*04 variant, HLA-DQB1*04:90, identified in a Chinese Han individual.

Identification of the novel HLA-DQB1*04:90 allele that differs from DQB1*04:01:01:01 at nucleotide 183 in exon 2 from A to T.

[Exclusion of HLA-C Genotype with Zero Mismatched PCR-SBT Results by Next Generation Sequencing].

Objective: Three cases of rare alleles of HLA-C with zero mismatched PCR-SBT results were analyzed by full-length sequencing to determine the true genotypes.

Methods: Three rare HLA-C alleles with zero mismatched PCR-SBT results were screened from clinical transplant matching samples, and the full-length sequence was detected by next-generation sequencing technology.

Results: The results of PCR-SBT typing of 3 samples were: HLA-C*03:04, 12:167; HLA-C*07:291, 15:02; HLA-C*01:43, 08:16.

HLA-DRB1*14:239, a novel HLA-DRB1 allele with one exonic mutation.

HLA-DRB1*14:239 differs from HLA-DRB1*14:03:01 by one nucleotide substitution in codon 82 in exon 2.