Immunohistochemical observation on luteinizing hormone in rat testes before and after testicular capsulotomy.

Aim: In the testicular capsulotomized rats, although there was a significant increase in the luteinizing hormone (LH) levels, the secretion of testosterone remained low. In order to clarify the mechanisms of this phenomenon, the binding of endogenous LH to the testes were observed before and after testicular capsulotomy.

Methods: Peroxidase-antiperoxidase (PAP) method was used to detect the binding of LH to the testes in rats.

Effect of testicular capsulotomy on lipid droplets in the seminiferous tubules of rats.

Aim: In order to reveal the histochemical alteration that might occur during the processes of the spermatogenic disruption induced by testicular capsulotomy, the location and alteration of lipid droplets in the seminiferous tubules were observed in the present study.

Methods: Osmium tetroxide was used to demonstrate the lipid droplets in the seminiferous tubules of capsulotomized and sham-operated control testes.

Results: In the seminiferous tubules of the sham-operated rat testes, many small lipid droplets were located close to the basement membrane of the seminiferous tubules.

Effect of testicular capsulotomy on fertility of rats.

Aim: To demonstrate the effect of capsulotomy on the fertility of male rats.

Methods: Testicular capsulotomy was carried out in immature (21 days) and adult (60 days) male rats. The fertility of them was assessed by cohabitation with proestrus females overnight and 20 days later, the females were examined for impregnation.

Effects of flavonoids from Semen Cuscutae on the reproductive system in male rats.

Aim: To evaluate the effects of the flavonoids extracted from the Semen Cuscutae (FSC) on the reproductive and endocrine functions in male rats.

Methods: (1) FSC were obtained from the semen of Cuscuta sinensis Lam through solvent extraction and polyamide columnar chromatography; (2) Effect of FSC on the reproductive organs was assessed in immature rats. Rats were administered FSC through gastric gavage at a dose of 300 mg/kg per day for 7 days and the weights of testis, epididymis, seminal vesicle and pituitary gland were then observed; (3) To observe the effect of FSC on the reproductive endocrine function: same dose level of FSC was given to male rats of different age groups for 7 days; on day 8, the plasma testosterone (T), estradiol (E2) and LH were determined by RIA, the specific binding of LH was estimated and the testes were weighed.

Studies on relationship between testicular capsule and sperm transport in rat testis.

Aim: In SD rats, histological changes in the testis were observed after bilateral capsulotomy (of the tunica albuginea) in order to investigate the physiological role of the testicular capsule on sperm transport.

Methods: Bilateral longitudinal capsulotomy was devised to disrupt the capsular contractile function. With this technique, only the tunica vaginalis and tunica albuginea were slit open, leaving the tunica vasculosa intact to embrace the underlying testicular parenchyma.

Effect of testicular capsulotomy on secretion of testosterone and gonadotrophins in rats.

Aim: In order to clarify further the mechanisms underlying the effect of capsulotomy on testicular function, the levels of testosterone, LH and FSH were observed.

Methods: Intratesticular testosterone levels and LH, FSH levels in the peripheral blood of normal, sham-operated and capsulotomized rats were detected by RIA.

Results: After testicular capsulotomy, there was a progressive reduction in the testosterone level in the testicular venous blood together with a progressive increase in the LH and FSH levels in the peripheral blood from approximately 30 days post-capsulotomy.

What is the significance of nasal involvement in nasopharyngeal carcinoma?

Purpose: The purpose of this study was to differentiate the patterns of nasal fossa involvement in nasopharyngeal carcinoma (NPC) and to clarify its prognostic influence on local control and survival after radiation therapy.

Methods And Materials: Between November 1989 and July 1991, 218 patients with histologically proven local-regional NPC were treated with radiotherapy following the protocol at the Department of Radiation Oncology, Cancer Hospital, Shantou University School of Medicine. All patients had pretreatment CT scans.