Standard: Human gastric cancer organoids.

Gastric cancer is one of the most common malignancies with poor prognosis. The use of organoids to simulate gastric cancer has rapidly developed over the past several years. Patient-derived gastric cancer organoids serve as in vitro models that closely mimics donor characteristics, offering new opportunities for both basic and applied research.

Biomimetic Strategies of Slip Sensing, Perception, and Protection in Prosthetic Hand Grasp.

This study develops biomimetic strategies for slip prevention in prosthetic hand grasps. The biomimetic system is driven by a novel slip sensor, followed by slip perception and preventive control. Here, we show that biologically inspired sensorimotor pathways can be restored between the prosthetic hand and users.

Programmable Wrinkled MXene-Based Soft Actuators with Moisture- and Light-Responsive Deformation and Water-Surface Locomotion Capabilities.

Soft actuators are limited by single-mode driving technology, which poses challenges in dealing with complex and multidimensional movements. In this study, a multiresponsive soft actuator was fabricated by integrating a microwrinkling structure into an MXene-based film, enabling programmable motions. To achieve this, we introduced -hexane into the film preparation process and utilized its rapid volatilization to accelerate the shrinkage difference between the film and the substrate.

Enhanced Water Splitting Electrocatalysis with Heterointerfacial Cobalt Phosphide In Situ Supported on a Cellulose-Derived Carbon Aerogel.

The active site density, intrinsic activity, and supporting substrate of cobalt phosphide catalysts are vital to their performance in alkaline water electrolysis. In this work, a CoP/CoP loaded on cellulose nanofiber-derived carbon aerogels (CP/CCAs) bifunctional electrocatalyst with a three-dimensional network and heterostructure is illustrated through sequential facile hydrothermal, freeze-drying, and phosphorylation processes. The three-dimensional network of carbon aerogels derived from cellulose nanofibers reveals a specific surface area of 183.

Effects of synbiotics surpass probiotics alone in improving type 2 diabetes mellitus: A randomized, double-blind, placebo-controlled trial.

Background And Aims: Combining probiotics and prebiotics in synbiotics may present a synergistic approach to improve type 2 diabetes mellitus (T2DM); however, further evidence is required to establish the comparative efficacy of synbiotics versus probiotics. This study aimed to evaluate the effects of Bifidobacterium animalis subsp. lactis MN-Gup (MN-Gup) and a synbiotic mixture of MN-Gup and galactooligosaccharide (MN-Gup-GOS) on glycemic control in T2DM patients and explore possible mechanisms.

Enhancing plant resilience: Nanotech solutions for sustainable agriculture.

The global population growth is driving up the demand for agricultural products, while traditional farming methods like those from the Green Revolution are becoming unsustainable due to climate change. To address these challenges and ensure agricultural sustainability, innovative techniques, such as nanotechnology, are essential to meet rising food demands and enhance agricultural sustainability. Nanotechnology, which promotes a more sustainable and resilient agricultural system while enhancing food security, is a key catalyst for the Agri-tech revolution.

Sequential delivery of IL-10 and icariin using nanoparticle/hydrogel hybrid system for prompting bone defect repair.

The treatment of large bone defects remains challenging due to the lack of spatiotemporal management of the immune microenvironment, inflammation response and bone remodeling. To address these issues, we designed and developed a nanoparticle/hydrogel hybrid system that can achieve the combined and sequential delivery of an anti-inflammatory factor (IL-10) and osteogenic drug (icariin, ICA). A photopolymerizable composite hydrogel was prepared by combining gelatin methacryloyl (GelMA) and heparin-based acrylated hyaluronic acid (HA) hydrogels containing IL-10, and poly(dl-lactide-co-glycolide) (PLGA)-HA nanoparticles loaded with ICA were incorporated into the composite hydrogels.

Matching P- and N-type Organic Electrochemical Transistor Performance Enables a Record High-gain Complementary Inverter.

The charge transport of channel materials in n-type organic electrochemical transistors (OECTs) is greatly limited by the adverse effects of electrochemical doping, posing a long-standing puzzle for the community. Herein, an n-type conjugated polymer with glycolated side chains (n-PT3) is introduced. This polymer can adapt to electrochemical doping and create more organized nanostructures, mitigating the adverse effects of electrochemical doping.

Assessing the correlation between park view elements and exercise physiological indicators of walkers, joggers, and runners: a case study of Century Park in Shanghai.

Objective: To explore the correlation between park view elements and their combinations on the heart rate (HR) and speed of walkers, joggers, and runners in different groups of people's profiles and walking types, provide suggestions for the planning and design of walking suitability of walking trails in parks, and guide people with different walking needs to scientifically choose walking trails in parks.

Methods: Profile data and exercise data of users who recorded walking activities in Century Park are collected on Strava, and the park view images (PVIs) were taken and segmented semantically. Data are grouped according to gender, age, weight and exercise type, and the quantitative relationship between HR, speed and 17 park view elements is studied by Spearman correlation analysis.

Tendon Groove Technique: A Double-Bundle Footprint Technique for Anterior Cruciate Ligament Reconstruction.

Double-bundle (DB) anterior cruciate ligament (ACL) reconstruction has biomechanical advantages over single-bundle reconstruction. However, most studies perform the DB reconstruction with 2 femoral tunnels, which fails to provide an entire femoral footprint for ACL reconstruction. In this study, we describe a femoral double-bundle footprint technique for ACL reconstruction, named the tendon groove technique.

Genetic variation in Arabidopsis thaliana reveals the existence of natural heat resilience factors for meiosis.

Heat interferes with multiple meiotic processes, leading to genome instability and sterility in flowering plants, including many crops. Despite its importance for food security, the mechanisms underlying heat tolerance of meiosis are poorly understood. In this study, we analyzed different meiotic processes in the Arabidopsis (Arabidopsis thaliana) accessions Columbia (Col) and Landsberg erecta (Ler), their F1 hybrids, and the F2 offspring under heat stress (37°C).

Development and clinical validation of photochemical biosensors for monitoring hemoglobin, blood lipids and uric acid in plateau areas.

High-altitude regions are prone to plateau erythrocytosis due to unique geo-climatic conditions such as low oxygen, high altitude, and low temperatures, with significantly higher incidences of hyperlipidemia and hyperuricemia compared to lowland areas. However, the extreme environmental conditions at high altitudes and the elevated hematocrit levels in blood samples from these populations present significant challenges to the applicability of existing point-of-care testing (POCT) devices. This study describes the development, early clinical validation, and potential clinical impact of three portable, paper-based photochemical biosensing platforms specifically designed for use at high altitudes to monitor hemoglobin, lipids, and uric acid in blood samples.

Identification and Characterization of the Y-Organ of Orange Mud Crab Scylla Olivacea.

The Y-organs (YOs) synthesize and secrete molting hormones, and thus regulate crustacean growth and reproduction. However, the YOs of the orange mud crab Scylla olivacea is yet to be described due to its minute size and ambiguous feature. This study describes the location, morphological characteristics, histology, and the changes of YOs at different molt stages, and examines in vitro ecdysteroid secreted by the YOs of S.

A bacterial methyltransferase that initiates biotin synthesis, an attractive anti-ESKAPE druggable pathway.

The covalently attached cofactor biotin plays pivotal roles in central metabolism. The top-priority ESKAPE-type pathogens, and , constitute a public health challenge of global concern. Despite the fact that the late step of biotin synthesis is a validated anti-ESKAPE drug target, the primary stage remains fragmentarily understood.

Evolutionary engineering of : Crafting a synthetic methylotroph via self-reprogramming.

Methanol, as a non-edible feedstock, offers a promising sustainable alternative to sugar-based substrates in biochemical production. Despite progress in engineering methanol assimilation in nonmethylotrophs, the full transformation into methanol-dependent synthetic methylotrophs remains a formidable challenge. Here, moving beyond the conventional rational design principle, we engineered a synthetic methylotrophic through genome rearrangement and adaptive laboratory evolution.

The optimal surgical time after stent placement in obstructive colorectal cancer: impact on long-term survival of patients.

Objective: To investigate the optimal interval between self-expanding metal stent (SEMS) placement and radical surgery in patients with obstructive colorectal cancer.

Method: In this study, a retrospective research design was used to select 125 patients with obstructive colorectal cancer who underwent colonoscopic SEMS placement with subsequent radical surgery between February 2011 and November 2022 at Shanghai Changhai Hospital. In addition, their clinical data and therapeutic efficacy were examined.

Genomic, transcriptomic, and metabolomic analyses reveal convergent evolution of oxime biosynthesis in Darwin's orchid.

Angraecum sesquipedale, also known as Darwin's orchid, possesses an exceptionally long nectar spur. Charles Darwin predicted the orchid to be pollinated by a hawkmoth with a correspondingly long proboscis, later identified as Xanthopan praedicta. In this plant-pollinator interaction, the A.

Big data tax collection and management, enterprise financialization and enterprise innovation: Quasi-natural test evidence based on the "Golden Tax Phase III".

In the era of digital economy, big data tax collection and management has become an important governance tool for digital government. In this study, the quasi-natural test environment provided by the "Golden Tax Phase III" policy launched in 2013 and the method of propensity score matching and differentiation (PSM-DID) were used on combination of the samples of A-share listed enterprises in Shanghai and Shenzhen during 2010-2021 to analyze and demonstrate the impact of this policy on the innovation of the listed enterprises. To ensure the robustness of these findings, various statistical techniques such as parallel trend tests, placebo tests, and the explained variable replacement were employed.

Identification of Brucella RS15060 as a novel type IV secretion system effector associated with bacterial virulence.

Brucella is an intracellular parasitic pathogen that causes the worldwide zoonotic disease brucellosis. The type IV secretion system (T4SS) is utilized to secrete various effectors to help Brucella form Brucella-containing vacuoles within the cell and accomplish intracellular trafficking and replication. Brucella has fewer recognized effector proteins than other intracellular parasites in the Proteobacteria, indicating that Brucella may contain a large number of unidentified effector proteins.

Supaglutide alleviates hepatic steatosis in monkeys with spontaneous MASH.

Background: Glucagon-like peptide 1 (GLP-1) is an incretin hormone and plays an important role in regulating glucose homeostasis. GLP-1 has a short half-life due to degrading enzyme dipeptidyl peptidase-IV and rapid kidney clearance, which limits its clinical application as a therapeutic agent. We demonstrated previously that supaglutide, a novel long-acting GLP-1 analog, exerted hypoglycemic, hypolipidemic, and weight loss effects in type 2 diabetic db/db mice, DIO mice, and diabetic monkeys.

Balanced water and heat energy recycling by full evaporation of wastewater (FEW) in dry biorefining processes of lignocellulose biomass.

Lignocellulosic biorefinery technology requires minimum energy consumption and wastewater generation to overcome challenges in industrial applications. This study established a rigorous model and a comprehensive physical property database of dry biorefining process on Aspen Plus platform for production including L-lactic acid, citric acid, sodium sugar acids, amino acid, and ethanol based on the experimental data. Full evaporation of wastewater (FEW) approach was proposed to completely replaced the external steam supply, and significantly reduced the freshwater input by 67% ∼ 85% and wastewater generation by 64% ∼ 89%, depending on the specific products.

A novel Brucella T4SS effector RS15060 acts on bacterial morphology, lipopolysaccharide core synthesis and host proinflammatory responses, which is beneficial for Brucella melitensis virulence.

Brucella relies on the type IV secretion system (T4SS) to establish replication niches within host cells. However, the Brucella T4SS effectors and their functions have not been fully identified. In this study, we investigated the function of Brucella RS15060, a novel T4SS effector discovered in our previous study, on the bacterial biological characteristics and pathogenesis by construction of the gene deletion and complementation strains.

Integrative study of lung cancer adeno-to-squamous transition in EGFR TKI resistance identifies RAPGEF3 as a therapeutic target.

Although adeno-to-squamous transition (AST) has been observed in association with resistance to epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) in clinic, its causality, molecular mechanism and overcoming strategies remain largely unclear. We here demonstrate that squamous transition occurs concomitantly with TKI resistance in PC9-derived xenograft tumors. Perturbation of squamous transition via DNp63 overexpression or knockdown leads to significant changes in TKI responses, indicative of a direct causal link between squamous transition and TKI resistance.