[Clinical analysis of complications of polyacrylamide hydrogel injection for augmentation mammoplasty in 118 cases].

Objective: To analyze the clinical manifestations and explore suitable treatments for the complications induced by polyacrylamide hydrogel(PAHG) injection for augmentation mammoplasty.

Methods: The symptoms, physical signs and treatments of the 118 cases were analyzed and summarized.

Results: The complications included in nodule formation (54.

Multi-factor analysis of initial poor graft function after orthotopic liver transplantation.

Background: In the early period of orthotopic liver transplantation (OLT), initial poor graft function (IPGF) is one of the complications which leads to primary graft non-function (PGNF) in serious cases. This study set out to establish the clinical risk factors resulting in IPGF after OLT.

Methods: Eighty cases of OLT were analyzed.

FDG-PET study of the bilateral subthalamic nucleus stimulation effects on the regional cerebral metabolism in advanced Parkinson disease.

The aim of the study was to evaluate the changes in regional cerebral metabolic rate of glucose (rCMRGlu) induced by bilateral subthalamic nucleurs (STN) stimulation in advanced Parkinson's disease (PD). 18F-Fluorodeoxyglucose (FDG) PET data obtained before and one month after stimulation were analyzed with statistical parametric mapping (SPM). As a result of clinically effective bilateral STN stimulation, rCMRGlu increased in lateral globus pallidus (GP), upper brain stem, dorsolateral prefrontal cortex (DLPFC) and posterior parietal-occipital cortex, and decreased in the orbital frontal cortex and parahippocampus gyrus (p < 0.

[Anomalous origin of the left coronary artery from the pulmonary artery: report on 10 cases].

Objective: The anomalous origin of the left coronary artery from the pulmonary artery (ALCAPA) is a rare congenital coronary artery abnormality. This study aimed to investigate the clinical characteristics, diagnosis and treatment of the disorder.

Methods: The medical data, including common clinical presentations, the findings of the digital subtraction angiogram (DSA), echocardiograms and the electrocardiograph (ECG), surgical records and the outcome, of 10 children with ALCAPA between June 2001 and February 2005 were retrospectively reviewed.

[Repair alveolar cleft bone defects with bone marrow stromal cells].

Objective: To explore the feasibility of repairing alveolar cleft bone defects with bone marrow stromal cells.

Methods: Total 7 patients of alveolar cleft were included in this study. The hBMSCs were isolated by percoll gradient centrifugation from patient's bone marrow aspirated from iliac crest.

Plain film arthrography applied to the diagnosis of intra-articular adhesions of the temporomandibular joint.

Purpose: To evaluate the diagnostic accuracy of plain film arthrography (PFA) on the presence of intra-articular adhesions of the temporomandibular joint (TMJ).

Patients And Methods: The study population included 105 consecutive patients (105 TMJs) and data collected in our department. All patients were examined by PFA and arthroscopy.

[Analysis of gene expression profiles in gastric cancer cell cycle].

Objective: To detect the gene expression profile in gastric cancer cell cycle and explain the mechanism of gastric cancer cell proliferation by a genomic study.

Methods: Gastric cancer cells MKN45 were synchronized at G2/M and G1/S point by nocodazole-thymidine and double thymidine methods. The synchronizing degree of cells was monitored by flow cytometry.

[The effect of PKC-delta inhibitor Rottlerin on human colon cancer cell line SW1116 and its mechanism].

Objective: To evaluate the effect of PKC-delta inhibitor Rottlerin on human colon cancer cells and its mechanism.

Methods: Human colon cancer cell line SW1116 cells were treated with Rottlerin. The transcriptional level of DNA methyltransferase (Dnmt)1, Dnmt3a and Dnmt3b was detected by real-time RT-PCR.

Alteration of hormone and neurotransmitter production in cultured cells by high and low frequency electrical stimulation.

HFS has become a widely used method in functional neurosurgery. However, its mechanism is not well understood, and its cellular and molecular effects have not yet been investigated. The aim of the study was to understand which cellular events, unrelated to the network organization of cells or neurons, participate in the mechanism of action of HFS.

[Experimental study of glucocorticoid in the treatment of acute respiratory distress syndrome induced by E.coli].

Objective: To evaluate the effect of glucocorticoid (GC) in the treatment of septic acute respiratory distress syndrome (ARDS).

Methods: ARDS model was reproduced by intraperitoneal injection of E.coli in piglets.

[Research of the preservation of the composite facial allograft].

Objective: To investigate the effective method of preserving composite facial allograft so as to attenuate ischemic injury.

Methods: The composite facial allografts were harvested from dog, perfused and preserved with 4 degrees C physiological sodium chloride and UW solution respectively. Immediately after the removal of the flap, after 12, 24, 48 h of preservation, MTT assay was used to determine the viability of several kinds of tissue, including skin, mucosa, muscle, bleed vessel, nerve and gland.

[Strategy study of harvesting total facial flap and donor choice for allograft transplantation in cadaver].

Objective: To explore the operational strategy of harvesting total facial allograft by autopsy.

Methods: Twelve fresh human cadavers were dissected. They were divided into two groups randomly.

[Effect of degradable products on degradable property of PGLA in vitro].

In order to investigate the effect of degradable products on degradable property of PGLA in vitro, two kinds of media-PBS and artificial plasma were prepared for immersing PGLA under changing or non-changing media condition. The mass loss rate of PLGA was calculated and the pH value in the non-changing media was measured before and after immersing 2 w, 3 w, 4 w, 6 w, 8 w and 10 w respectively. The results showed that there was almost no statistically significant difference of mass loss rate of PGLA immersing in two kinds of media at 2 w (P > 0.

Autologous Ross operation for congenital aortic stenosis.

Congenital aortic stenosis is a relatively common cardiac anomaly encountered in approximately 5% of all children with heart disease. The Ross procedure is increasingly used for replacement of the aortic valve in children. We report a 12-year-old boy who was born with congenital aortic stenosis secondary to a bicommissural aortic valve.

[Establishment of composite facial and scalp allograft transplantation model in canine].

Objective: To develop an experimental model of composite facial and scalp allograft in canine in order to investigate technical and immunological aspects and functional recovery of facial muscles of this new approach to facial reconstruction.

Methods: (1) Anatomic study: Four mongrel dogs were used for anatomical dissection of the head and neck region and for harvesting flap experiment. (2) Autologous transplantation (group I): Three types composite facial and scalp autologous transplantation were performed in five mongrel dogs.

[Effects of perinatal exposure to methylmercury on the structure of hippocampus and cerebellum in young rats].

Objective: To evaluate the effects of perinatal exposure to methylmercury on the structure of hippocampus and cerebellum in young pups.

Methods: Divided the 9 pregnant SD rats into three groups randomly: group I, II and III After exposing the rats of the three groups from day 7 in pregnancy through lactation with methylmercury (the dose were 0, 0.1 and 1mg/(kg x d) respectively), examined the early physiology development and the structure of hippocampus and cerebellum of day-21-offspring rats.

[In vitro study on proliferation and multi-lineage differentiation potential of adipose-derived cells].

Adipose were obtained from patients underwent liposuction treatment (total 19 female donors, 31.5 +/- 5.8 years old).

A case-control study of Guillain-Barré syndrome in Harbin, China.

We evaluated the putative factors for the onset of Guillain-Barré syndrome (GBS) in Harbin, China by a case-control study based on the information from GBS patients identified from a population-based incidence survey, which is the first study of this kind in China. Sixty-nine GBS patients were identified during a 1-year period from 1 October 1997 to 30 September 1998, and they were matched with 69 controls for gender and age (+/-5 years). GBS diagnosis was validated by senior neurologists and GBS patients were followed up for 6 months after onset.

Clinical significance of human kallikrein 10 gene expression in colorectal cancer and gastric cancer.

Background And Aim: Recent evidence suggests that the human kallikrein 10 (KLK10) gene is differentially regulated in endocrine-related tumors and has potential as diagnostic and/or prognostic marker; however, KLK10 expression has never been investigated in gastrointestinal cancers. The aims of this study were to demonstrate expression and single nucleotide polymorphisms of KLK10 in colorectal cancer (CRC) and gastric cancer (GC), and to correlate the relative KLK10 expression level with clinicopathological factors of CRC and GC.

Methods: Between March 2004 and January 2005, 63 patients with histologically confirmed CRC and 36 with GC were recruited into the study.

Cathepsin K in adipocyte differentiation and its potential role in the pathogenesis of obesity.

Context: The alteration of protein expression in white adipose tissue (WAT) may contribute to the pathogenesis of obesity.

Objective: The aim of the present study was to uncover proteins differentially expressed in the WAT of overweight/obese subjects and study the role of the identified proteins in adipocyte differentiation.

Design And Setting: Two-dimensional electrophoresis and matrix-assisted laser desorption ionization-time of flight-mass spectrometry were used to identify proteins differentially expressed in WAT between obese/overweight and control groups.

Diagnosis of congenital obstructive aortic arch anomalies in Chinese children by contrast-enhanced magnetic resonance angiography.

Objective: The purpose of this study was to evaluate the accuracy of contrast-enhanced magnetic resonance angiography for the diagnosis of congenital obstructive aortic arch anomalies in children and compare it with transthoracic echocardiography and other MR imaging techniques (ECG gated T1-weighted spin-echo imaging and gradient-echo cine imaging).

Materials And Methods: Contrast-enhanced magnetic resonance angiography, ECG gated T1-weighted spin-echo imaging, and gradient-echo cine imaging were performed for the diagnosis of congenital obstructive aortic arch anomalies in 416 patients from April 1999 to March 2005 (age range, 3 days to 12 years; mean age, 2.4 years) using a GE 1.

[Facial nerve monitoring in parotid gland surgery].

Objective: To investigate the use and effect of facial nerve monitoring in parotid gland surgery.

Method: Twenty-two patients with benign parotid tumor who received parotidectomy with continuous monitoring of the facial nerve were selected to analyse.

Result: Each patient had a total resection of tumor without facial nerve paresis or sialocele formation.

[Mitosis arrest caused by inhibition of PLK1 expression in gastric cancer MKN45 cells].

Objective: To observe the effect of polo-like kinase 1 (PLK1) gene depletion on mitosis phenotype and elucidate its vital role in gastric cancer cell line (MKN45) mitosis.

Methods: The PLK1 expression in MKN45 cells was blocked by RNA interference (RNAi), the expression level of PLK1 mRNA and protein were measured by real-time quantitative PCR and Western blot, respectively. The morphological change of microtubules and mitosis phenotype in MKN45 cells were observed by immunofluorescence staining and laser confocal microscopy, the morphological changes of cells were observed by reverse microscopy, the variation of cell cycle distribution was detected by flow-cytometry.

Targeting study of gelatin adsorbed clodronate in reticuloendothelial system and its potential application in immune thrombocytopenic purpura of rat model.

Depletion of splenic and hepatic macrophages has potentials to alleviate hemorrhage in patients who suffered from immune thrombocytopenic purpura (ITP). This investigation was aimed to assess whether nanotechnology can play a role in this clinical setting by absorbing bisphosphonate clodronate (CLOD) to type A gelatin nanospheres (GNS) to form CLOD-GNS. First, the stability of CLOD-GNS was assessed in vitro and up to 6 mg CLOD can be adsorbed in 1 mg GNS.