Comprehensive multi-tissue epigenome atlas in sheep: A resource for complex traits, domestication, and breeding.

Comprehensive functional genome annotation is crucial to elucidate the molecular mechanisms of agronomic traits in livestock, yet systematic functional annotation of the sheep genome is lacking. Here, we generated 92 transcriptomic and epigenomic data sets from nine major tissues, along with whole-genome data from 2357 individuals across 29 breeds worldwide, and 4006 phenotypic data related to tail fat weight. We constructed the first multi-tissue epigenome atlas in terms of functional elements, chromatin states, and their functions and explored the utility of the functional elements in interpreting phenotypic variation during sheep domestication and improvement.

Oral Intake of Collagen and Collagen Hydrolysate From Attenuates Ultraviolet-Induced Skin Photoaging in Mice.

With the rapid emergence of pufferfish aquaculture and processing industries, fish skin is underutilized as a byproduct of processing, leading to resource waste. In this study, skin collagen (TBSC) was extracted by acetic acid solubilization and its physicochemical properties were analyzed. The effects of TBSC and the TBSC hydrolysate (TBSCH) on ultraviolet (UV) irradiation-induced photoaging were investigated using a mouse model.

Habitat Occupancy of the Critically Endangered Chinese Pangolin () Under Human Disturbance in an Urban Environment: Implications for Conservation.

Globally, urban expansion has led to habitat fragmentation and altered resource availability, thus posing significant challenges for wildlife. The Chinese pangolin () is a critically endangered species experiencing population decline due to illegal trade and habitat degradation. This study analyzed variables affecting habitat occupancy of Chinese pangolins using a single-season occupancy model across 134 study grids (600 m × 600 m) in peri-urban areas of Dharan Sub-Metropolitan City, eastern Nepal.

Redescription of (Nichols, 1926) with the description of a new species of the genus (Cypriniformes, Gobionidae) from southern China.

Although (Nichols, 1926) has been treated valid since it was described, its morphology remains vague, especially when comparing it with another similar species, (Yao & Yang, 1977). In this study, the types of both species were examined and also compared with several lots of specimens from a wide geographical range: there is no significant difference in morphology between them. Additionally, molecular evidence supported by mitochondrial gene sequence also showed low genetic distance in between.

A new species of (Teleostei, Cypriniformes, Xenocyprididae) from Southeast China.

is described from the Qiantang and Oujiang rivers in Zhejiang Province and a tributary of the Yangtze River adjacent to the Qiantang River. It is distinguished from congeners by the following combination of morphological features: no obvious anterior notch on the tip of the upper lip; 45-52 lateral-line scales; 18-21 pre-dorsal scales; two rows of pharyngeal teeth; a maxillary extending to or slightly beyond the vertical anterior margin of the orbit in adult males; a pectoral fin extending to the pelvic fin in adult males; nuptial tubercles on the cheeks and lower jaw of males, which are usually united basally to form a plate; uniform narrow pale pink cross-bars on trunk and two widening significantly on caudal peduncle. Its validity was also supported by its distinct Cyt gene sequence divergence from all congeners and its monophyly recovered in a Cyt gene-based phylogenetic analysis.

DNA barcoding, identification, and validation of the pufferfish (Order: Tetraodontiformes) in China coastal waters.

The order Tetraodontiformes are one of the most unique groups of teleostean fish, exhibiting highly derived and greatly diversified phenotypes. It is a difficult task for both professionals and nonprofessionals to accurately identify these species only according to morphological characteristics. DNA barcoding can identify species at the molecular level to overcome the limitations of morphological classification.

New species and records of Pseudochironomini Sæther, 1977 (Diptera, Chironomidae) from the Dominican Republic.

Background: Pseudochironomini is a relatively small and poorly-studied tribe of subfamily Chironominae (Diptera, Chironomidae).

New Information: Andersen & Baranov is described and figured, based on a single male collected in a light trap at Matadero, Dominican Republic. The species can be separated from its congeners by the combination of the following characters: wing without dark bands, dorsocentrals in partly double row and apex of superior volsella rounded.

Prediction of death rates for cardiovascular diseases and cancers.

Background: To estimate cardiovascular and cancer death rates by regions and time periods.

Design: Novel statistical methods were used to analyze clinical surveillance data.

Methods: A multicenter, population-based medical survey was performed.

Decellularized diseased tissues: current state-of-the-art and future directions.

Decellularized matrices derived from diseased tissues/organs have evolved in the most recent years, providing novel research perspectives for understanding disease occurrence and progression and providing accurate pseudo models for developing new disease treatments. Although decellularized matrix maintaining the native composition, ultrastructure, and biomechanical characteristics of extracellular matrix (ECM), alongside intact and perfusable vascular compartments, facilitates the construction of bioengineered organ explants in vitro and promotes angiogenesis and tissue/organ regeneration in vivo, the availability of healthy tissues and organs for the preparation of decellularized ECM materials is limited. In this paper, we review the research advancements in decellularized diseased matrices.

, a new species of hillstream loach (Teleostei, Cypriniformes, Gastromyzontidae) from the Ou-Jiang River, Southeast China.

, a new species, is described from the Ou-Jiang basin in Zhejiang Province, Southeast China. It is distinguished from other species of the genus by having a combination of the following characteristics: body without obvious mottling; snout length longer than postorbital length; abdominal scaleless area extending to middle of pectoral-fin base; shorter rostral barbels, the outermost pair length 112.9%-140.

Effects of high-temperature short-time processing on nutrition quality of Pacific saury () using extracted fatty acids as the indicator.

Microwave thermal processing is a promising technology to greatly improve product quality by achieving high-temperature short-time (HTST) processing for solid foods. And the non-thermal effect of microwave fields on nutritional quality is a major public concern. To distinguish the non-thermal effect of microwave fields, the thermal effect of HTST processing should be revealed first.

Optimization of the "" mate-pair method improves contiguity and accuracy of genome assembly.

A combination of short-insert paired-ended and mate-pair libraries of large insert sizes is used as a standard method to generate genome assemblies with high contiguity. The third-generation sequencing techniques also are used to improve the quality of assembled genomes. However, both mate-pair libraries and the third-generation libraries require high-molecular-weight DNA, making the use of these libraries inappropriate for samples with only degraded DNA.

Consumers' purchase intention of wild freshwater fish during the COVID-19 pandemic.

The association between the COVID-19 pandemic and the wildlife trade in the seafood market in Wuhan has raised public concern regarding wildlife consumption and public health safety. Considering several coronavirus transmission incidents related to aquatic products and the location of wild freshwater fish in aquatic consumption in China, the effects of COVID-19 on the purchase intention of wild freshwater fish was investigated. Based on 1163 online questionnaires from eight provinces (including two province-level municipalities) in the Yangtze River Basin, ordered logistic regression was carried out to analyze the influencing factors of purchase intention of wild freshwater fish during the COVID-19 pandemic.

Retrospective stable isotopes of vertebrae reveal sexual ontogenetic patterns and trophic ecology in oceanic whitetip shark, .

There is a common phenomenon in nature whereby some animals have differences in their ontogenetic changes in dietary preferences between sexes, especially apex predators. These reflect changes in the needs of development during their lifetimes. Apex predators potentially have diverse dietary niches and a large impact on the trophic dynamics within ecosystems.

Optimization of combined microwave and hot air drying technology for purple cabbage by Response Surface Methodology (RSM).

Purple cabbage is one of the world's most widely consumed vegetables with high nutritional values containing the antioxidants and anti-inflammatory activity of anthocyanins, vitamins, and minerals. But due to rapid postharvest quality decay, novel processing techniques including drying are required. In order to determine the conditions of combined microwave and hot air drying for purple cabbage, factors affecting the drying process including microwave density, hot air temperature, and the dry base water content at conversion point were investigated using the anthocyanin content, DPPH antioxidant capacity, chewiness, , rehydration ratio, and average drying rate as responses.

Thermally induced isomerization of linoleic acid and α-linolenic acid in Tratt seed oil.

seed oil is obtained from the seeds left following pressing of the juice from fruit. The total oil content of seed was around 9.30%.

Gut microbiomes of bigheaded carps and hybrids provide insights into invasion: A hologenome perspective.

Gut microbiomes play an essential role in host survival and local adaptation and thus can facilitate the invasion of host species. Biological invasions have been shown to be linked to the genetic properties of alien host species. It is thus plausible that the holobiont, the host, and its associated microbiome act as an entity to drive invasion success.

Nonvolatile taste compounds of Shanghai smoked fish: A novel three stages control techniques.

In this work, the effect of processing stages including first soaking (FS), frying after first soaking (FFS), and second soaking (SS) on nonvolatile taste compounds of Shanghai smoked fish was investigated using high-performance liquid chromatography (HPLC) and automatic amino acid analyzer. Results showed that the contents of free amino acids (FAAs) ranged from 396.94 to 585.

Complete mitochondrial genome of the freshwater fish (Teleostei, Cyprinidae): genome characterization and phylogenetic analysis.

The cyprinid genus Günther, 1896 consists of 24 valid species distributed in Southeast Asia, including Taiwan, Hainan, mainland China and the Indochina region. In the present study, we determined the complete mitochondrial genome of , which is 16,598 bp in length, containing 13 protein-coding genes, two rRNA genes, 22 tRNA genes and a typical control region (D-loop). To verify the molecular phylogeny of the subfamily Acrossocheilinae, we provide new insights to better understand the taxonomic status of , and .

Reidentification of and (Carangidae) reveals inconsistencies with current morphological taxonomy in China.

and are economically important pelagic fish species that are widely distributed in tropical and subtropical seas. The two species are often mistakenly identified due to their morphological similarities as described in the Chinese literature on fish identification. In this study, and samples were collected in the Eastern Indian Ocean and the South China Sea and reidentified using morphological and DNA barcoding techniques.

Pleistocene isolation caused by sea-level fluctuations shaped genetic characterization of over a large-scale geographical distribution.

The southern lesser pomfret () is an economically important fish, and its numbers are declining because of overfishing and environmental pollution. In addition, owing to the similarities of its external morphological characteristics to other species in the genus , it is often mistaken for grey pomfret () or silver pomfret () juveniles. In this study, the genetic diversity and structure of 264 individuals from 11 populations in China and Malaysia coastal waters were evaluated for the first time, to the best of our knowledge, using mitochondrial cytochrome b fragments.

Effects of microwave time on quality of grass carp fillets processed through microwave combined with hot-air drying.

In this study, the quality changes of grass carp fillets dried by microwave-hot-air combined drying under different microwave time were investigated. The salted fillets were dried at 385 W microwave with different time (0, 2, 4, 6, 8, and 10 min), followed by 65℃ hot air to the end. The quality of fillets was evaluated by drying time, color, hardness, rehydration ratio, and taste characteristics (ATP-related compounds, free amino acids, E-tongue taste profile, EUC, and TAV).

Evolution and genetics of bighead and silver carps: Native population conservation versus invasive species control.

Bighead carp () and silver carp (, collectively called bigheaded carps, are cyprinids native mainly to China and have been introduced to over 70 countries. Paleontological and molecular phylogenetic analyses demonstrate bighead and silver carps originated from the Yangtze-Huanghe River basins and modern populations may have derived from the secondary contact of geographically isolated fish during the last glacial events. Significant genetic differences are found among populations of native rivers (Yangtze, Pearl, and Amur) as well as introduced/invasive environments (Mississippi R.