Effects of dietary carbohydrate and lipid levels on growth performance, hepatic histology and antioxidant capacity, and flesh texture of mandarin fish ().

In this study, nine isonitrogenous experimental diets containing graded level of carbohydrates (40 g/kg, 80 g/kg and 120 g/kg) and crude lipid (80 g/kg, 120 g/kg, and 160 g/kg) were formulated in a two-factor (3×3) orthogonal design. 945 mandarin fish with similar body weight were randomly assigned into 27 tanks and the experiment diets were fed to triplicate tanks twice daily for 10 weeks. Results showed that different dietary treatments did not significantly affect survival rate and growth performance of mandarin fish.

Dynamics of bacterial composition and association with quality formation and histamine accumulation during Antarctic krill fish sauce fermentation.

In this study, we first verified the feasibility of using Antarctic krill as a raw material for quick-brewed fish sauce, and the results showed that the amino acid nitrogen (AAN)and total soluble nitrogen(TSN) in the fermented 30 days fish sauce met the national level fish sauce standards, but the histamine content exceeded the maximum limit value of histamine in fish sauce (400 mg/kg) set by the International Codex Alimentarius Commission (CAC) and the European Union (EU). On this basis, the correlation between physicochemical indexes and bacterial communities during the fermentation of fish sauce was investigated using high-throughput sequencing, culture method. The correlation analyses revealed the relationship between bacterial communities and the quality and safety of fish sauce, and these studies will be helpful for the development of Antarctic krill fish sauce.

A comprehensive RNA virome identified in the oyster Magallana gigas reveals the intricate network of virus sharing between seawater and mollusks.

Background: As a globally farmed oyster species, Magallana gigas has garnered significant attention due to the contaminated RNA viruses that have caused illness in humans. However, limited knowledge is available on the bioaccumulation status and overall diversity of RNA virome in the M. gigas digestive tissues (DTs).

Transcriptomics, metabolomics and proteomics analyses reveal glyphosate tolerance mechanism in red swamp crayfish Procambarus clarkii.

Glyphosate (Gly), the world's most widely used herbicide in agriculture, can poison the red swamp crayfish, Procambarus clarkii, via spray drift and surface runoff into surface waters. However, there is a paucity of research on the mechanisms that affect crayfish tolerance to Gly at typical environmental concentrations. To address this research gap, we investigated the effects of Gly stress (0, 6, 12, 24, and 72 h) at different concentrations (0, 1.

A chromosome-level genome assembly of skipjack tuna, Katsuwonus pelamis (Perciformes: Scombridae).

Skipjack tuna (Katsuwonus pelamis), a highly migratory pelagic species widely distributed in tropical and subtropical oceanic regions, has consistently ranked third in global fishery landings from 2015 to 2022 and holds substantial economic significance for the coastal fisheries of Pacific Rim countries. Integrating PacBio and Hi-C data, a chromosome-level assembly of its genome was accomplished. This assembly comprises 24 pseudo-chromosomes, yielding a genome size of 827.

Effects of perfluorooctanoic acid and nano titanium dioxide on the immune response and energy allocation in Mytilus coruscus.

Perfluorooctanoic acid (PFOA) functions as a surfactant, while nano-titanium dioxide (nano-TiO) serves as an antibacterial agent. These substances are extensively utilized in industrial production and, upon release into aquatic environments, pose significant threats to the viability and development of marine organisms. However, research into the effects of PFOA and nano-TiO on the immune functions and cellular energy allocation (CEA) of bivalves remains limited.

Trust in nutrition, subjective norms and urban consumers' purchase behavior of quinoa products: explanation based on preference heterogeneity.

Introduction: With the increase of disposable income and the awakening of health consciousness of Chinese residents, higher requirements have been put forward for the nutritional value of food. To meet the market demand and promote the high-quality development of the whole quinoa industry chain, this study aimed to analyze the purchase behavior of quinoa from the aspects of nutritional trust and subjective norms.

Methods: Based on 1,078 micro-survey data from 16 administrative districts in Shanghai, this paper empirically examined the influence of trust in nutrition and subjective norms on consumers' purchase behavior and willingness to pay of quinoa products, and further investigated the moderating effect of preference heterogeneity.

The Protective Effects of Microbe Derived Antioxidants on Digestive Tissue Morphology, Functions, and Intestinal Microbiota Diversity of Exposed to Glyphosate.

The use of glyphosate (Gly) has caused unnecessary economic losses to the aquaculture industry, but research on the effect of Gly on is very limited. The aim of this study is to reduce the negative effects of Gly, reduce yield loss, and improve economic benefits through nutritional feed control technology. The experiment involved 80 crabs randomly divided into four groups: control group, Gly group (48.

Changes in soluble sugars and the expression of sugar transporter protein genes in strawberry crowns responding to infection.

Unlabelled: Strawberry ( × ) production has been greatly hampered by anthracnose crown rot caused by . Crown, the modified stem of strawberry, is a sink organ involved in sugar allocation. Some Sugar Transport Proteins (STPs) are involved in competition for sugars between pathogen and host.

Habitat Occupancy of the Critically Endangered Chinese Pangolin () Under Human Disturbance in an Urban Environment: Implications for Conservation.

Globally, urban expansion has led to habitat fragmentation and altered resource availability, thus posing significant challenges for wildlife. The Chinese pangolin () is a critically endangered species experiencing population decline due to illegal trade and habitat degradation. This study analyzed variables affecting habitat occupancy of Chinese pangolins using a single-season occupancy model across 134 study grids (600 m × 600 m) in peri-urban areas of Dharan Sub-Metropolitan City, eastern Nepal.

Detection of Critical Parts of River Crab Based on Lightweight YOLOv7-SPSD.

The removal of back armor marks the first stage in the comprehensive processing of river crabs. However, the current low level of mechanization undermines the effectiveness of this process. By integrating robotic systems with image recognition technology, the efficient removal of dorsal armor from river crabs is anticipated.

Highly Efficient Methods with a Generalized Linear Mixed Model for the Quantitative Trait Locus Mapping of Resistance to Columnaris Disease in Rainbow Trout ().

Linear mixed models (LMMs) are commonly used in genome-wide association studies (GWASs) to evaluate population structures and relatedness. However, LMMs have been shown to be ineffective in controlling false positive errors for the analysis of resistance to Columnaris disease in Rainbow Trout. To solve this problem, we conducted a series of studies using generalized linear mixed-model association software such as GMMAT (v1.

Methoprene-Tolerant (Met) Acts as Methyl Farnesoate Receptor to Regulate Larva Metamorphosis in Mud Crab, .

The conserved role of juvenile hormone (JH) signals in preventing larvae from precocious metamorphosis has been confirmed in insects. Crustaceans have different metamorphosis types from insects; we previously proved that methyl farnesoate (MF) can prohibit larvae metamorphosis in mud crabs, but the molecular signal of this process still needs to be elucidated. In this study, () of was obtained and characterized, which we named .

Effects of Ozone Gas and Slightly Acidic Electrolyzed Water on the Quality of Salmon () Fillets from the Perspective of Muscle Protein.

To elucidate the mechanisms of ozone gas (OG) and slight acid electrolyzed water (SA) on the quality changes in texture, water-holding capacity, and softening of salmon, the bacterial growth, total volatile basic nitrogen, thiobarbituric acid reactive substance, a* value, texture properties, carbonyl content and free sulfhydryl content, myofibrillar fragmentation index, and proteolytic activities of salmon treated by OG (1 mg/m for 10 min) and SA (ACC 30 mg/L, 5 min) individually and in combination were studied. The results showed that total viable counts of SA + OG (dipped in SAEW for 5 min, followed by exposure to ozone for 10 min) was about 3.36 log CFU/g lower than the control (CK) (dipped in distilled water for 5 min) on day 10.

Physiological Responses of Juvenile Bullfrogs () to Salinity Stress.

Bullfrogs () are increasingly farmed for their high nutritional value and adaptability to intensive aquaculture systems. However, salinity stress due to environmental changes and habitat salinization poses a significant challenge for both wild and farmed bullfrogs. This study examines the physiological responses of juvenile bullfrogs to varying salinity levels (0, 2, 4, 6 ppt) to better understand their capacity for osmoregulation and adaptation to salinization.

RNA-Sequencing Analysis of the Viral Community in Yellow Catfish () in the Upper Reaches of the Yangtze River.

Different viruses are abundant in aquatic ecosystems. There has been limited research on the viral communities in the upper reaches of the Yangtze River. Yellow catfish (), an important economic fish that is widely distributed in the upper reaches of the Yangtze River, was selected as the research object.

Synthetic and Pharmacological Activities of Alantolactone and its Derivatives.

Alantolactone, a sesquiterpene lactone, is isolated from the traditional Chinese medicinal herb Inula helenium L. (Asteraceae). Alantolactone is known as its wide spectrum of biological effects, including antimicrobial, antifungal, antiviral, anthelmintic activities, anti-inflammatory activities, and antiproliferative effects on several cancer cell lines.

The metabolic characteristics and environmental adaptations of the intertidal bacterium sp. LCG004.

The intertidal zone, a dynamic interface of marine, atmospheric, and terrestrial ecosystems, exposes microorganisms to rapid shifts in temperature, salinity, and oxidative stress. Strain LCG004, representing a novel species, was isolated from the Lu Chao Harbor's intertidal seawater in the Western Pacific Ocean. The genome of the organism reveals its metabolic versatility, enabling the utilization of various organic substrates-ranging from organic acids, amino acids, to sugars, and encompassing complex carbohydrates-as well as adept handling of inorganic nutrients, thereby highlighting its significant role in the cycling of nutrients.

Lyophilized T Cell Reference Materials with Quantified Proportions of Subtypes.

The accurate quantification of T cell subtypes and their proportions is of great significance in cell-based biomanufacturing, diagnosis, and advanced therapy. The development and application of a cell reference material (RM) provide a solid foundation for reliable and consistent T cell quantification worldwide. However, creating a cell RM that is both accurate and practical remains a challenge.

1+1<2: Combined effect of low temperature stress and salt stress on Sesuvium portulacastrum L.

To expedite the deployment of Sesuvium portulacastrum floating bed technology in Hangzhou Bay and the Yangtze River Estuary, and to overcome the cryogenic constraint, our study concentrated on investigating the impacts of both individual and combined stress factors, particularly low temperature and salinity, on its application. We detected the S. portulacastrum related enzyme activity and other biological macromolecules under low temperature stress, salt stress and combined stress.

Adaptive expert fusion model for online wind power prediction.

Wind power prediction is a challenging task due to the high variability and uncertainty of wind generation and weather conditions. Accurate and timely wind power prediction is essential for optimal power system operation and planning. In this paper, we propose a novel Adaptive Expert Fusion Model (EFM+) for online wind power prediction.

Histopathological and histochemical analysis of the digestive tract of adult horseshoe crabs under mercury stress.

Mercury (Hg) contamination is a major environmental concern that continuously impacts marine organisms, including the mangrove horseshoe crab (Carcinoscorpius rotundicauda). As a vulnerable species, C. rotundicauda holds significant ecological and evolutionary value, making it particularly susceptible to Hg pollution and deserving of focused conservation efforts.

A newly characterized CFSH gene in sex chromosomes is associated with growth instead of sexual development in the prawn Macrobrachium rosenbergii.

Crustacean female sex hormone (CFSH) was first identified as a female eyestalk-specific factor involved in the female sexual development in blue crab. Whether CFSH has conserved role in other decapod species remains to be clarified. In this study, we identified a CFSH gene (MrCFSH) in the Z and W chromosomes from the prawn Macrobrachium rosenbergii genome.

Morphometric and phylogenetic analysis of a commercial fish (Teleostei, Leiognathidae).

The complete mitochondrial genome and phylogenetic analysis for from the South China Sea was performed using shallow genome skimming. For accurate species identification and redescription, morphometric and meristic characters were examined and compared with previous descriptions. To facilitate the identification of species and to enable comparison with the mitochondrial genome phylogeny, molecular comparisons were conducted using three mitochondrial genes: large ribosomal RNA ( rRNA), cytochrome c oxidase subunit 1 (), and NADH dehydrogenase ().

Carvacrol-loaded emulsions stabilized by soy protein isolate/chitosan oligosaccharide conjugates improved the quality of refrigerated sea bass (Lateolabrax maculatus).

In this study, the soybean protein isolate / chitosan oligosaccharide (SPI/COS) conjugates were developed using glycosylation and used as a stabilizer of carvacrol-loaded emulsions for refrigerating sea bass (Lateolabrax maculatus). The results of Fourier transform infrared spectroscopy and fluorescence spectroscopy showed that COS modified the secondary and tertiary structure of SPI by covalent binding. The SPI/COS conjugate with a mass ratio of SPI and COS of 4:1 (SPI/COS-4) exhibited high glycosylation degree, great water solubility and better emulsification properties.