133 results match your criteria: "Shanghai Medical College-Fudan University[Affiliation]"

Background: Carotid endarterectomy (CEA) is widely used to treat carotid artery stenosis (CAS). However, the effects of CEA on unilateral CAS-induced cognitive impairment and the underlying mechanism remain poorly understood.

Methods And Results: Thirteen patients diagnosed with unilateral severe CAS underwent pre- and post-CEA assessments, including fluoro-2-deoxy-d-glucose positron emission tomography/magnetic resonance imaging, cognitive assessments, and routine blood tests before and after CEA.

View Article and Find Full Text PDF

Cis-Regulation of an mA Eraser by an Insertion Variant Associated with Survival of Patients With Non-Small Cell Lung Carcinoma.

Adv Sci (Weinh)

December 2024

Department of Epidemiology, School of Public Health, Key Laboratory of Public Health Safety, Ministry of Education, Fudan University, Shanghai, 200032, China.

N6-methyladenosine (mA) serves as one of the crucial RNA modifications for genes involved in cancer progression. Here, 7273 expression quantitative trait loci potentially regulating 30 m6A pathway genes are identified from the GTEx database, with 69 single nucleotide polymorphisms significantly associated with survival of non-small cell lung carcinoma (NSCLC) patients (n = 1523) from the ongoing genome-wide association study after false positive probability tests. Notably, the rs151198415 locus, situated in a potential enhancer region, demonstrated a prolonged survival effect with the C>CCACG insertion, which is validated in an independent prospective cohort (n = 237), yielding a pooled hazard ratio of 0.

View Article and Find Full Text PDF

Discovery of novel small molecules targeting hepatitis B virus core protein from marine natural products with HiBiT-based high-throughput screening.

Acta Pharm Sin B

November 2024

Key Laboratory of Medical Molecular Virology (MOE/NHC), Research Unit of Cure of Chronic Hepatitis B Virus Infection (CAMS), Shanghai Frontiers Science Center of Pathogenic Microbes and Infection, School of Basic Medical Sciences, Shanghai Medical College Fudan University, Shanghai 200032, China.

Article Synopsis
  • Current anti-HBV therapies have limitations, leading researchers to explore HBV core protein assembly modulators (CpAMs) as potential new treatments.
  • The study developed a high-throughput screening system to identify novel CpAMs from a marine chemicals library, discovering a promising compound derived from naamidine J with effective anti-HBV activity.
  • This compound not only inhibited HBV replication in cell models but also showed a synergistic effect with existing treatments and proved to be safe in mouse models, indicating its potential for future anti-HBV therapies.
View Article and Find Full Text PDF

Augmented epigenetic repression of hepatitis B virus covalently closed circular DNA by interferon-α and small-interfering RNA synergy.

mBio

December 2024

Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Research Unit of Cure of Chronic Hepatitis B Virus Infection (CAMS), Shanghai Frontiers Science Center of Pathogenic Microbes and Infection, School of Basic Medical Sciences, Shanghai Medical College Fudan University, Shanghai, China.

Unlabelled: The persistence of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) is a key obstacle for HBV cure. This study aims to comprehensively assess the effect of interferon (IFN) and small-interfering RNA (siRNA) combination on the cccDNA minichromosome. Utilizing both cell and mouse cccDNA models, we compared the inhibitory effects of IFNα, siRNA, and their combination on cccDNA activity and assessed its epigenetic state.

View Article and Find Full Text PDF

3D visualization of cellular and molecular distributions in human crystalline lenses at different ages.

Am J Transl Res

October 2024

Department of Integrative Medicine and Neurobiology, School of Basic Medical Sciences, Institutes of Brain Science, Brain Science Collaborative Innovation Center, State Key Laboratory of Medical Neurobiology, Institute of Acupuncture and Moxibustion, Fudan Institutes of Integrative Medicine, Fudan University Shanghai 200032, China.

Article Synopsis
  • The study focused on the human lens, highlighting its complex structure and the need for insights into its 3D cellular organization as it ages.
  • Researchers used various tissue transparency methods to analyze human lenses from infants to seniors, revealing consistent equator proportions but different cellular patterns depending on age.
  • Notable findings included increased accumulation of equatorial epithelium in adolescents and changes in cell composition, alongside a molecular analysis that showed variations in specific proteins across different age groups.
View Article and Find Full Text PDF

Primary seminal vesicle lymphoma is a remarkably rare condition, predominantly manifesting as diffuse large B-cell lymphoma. Due to its rarity and nonspecific clinical presentations, it is often misdiagnosed or overlooked. Here, we report a case of a 68-year-old male diagnosed with primary seminal vesicle lymphoma, coinciding with prostate cancer.

View Article and Find Full Text PDF

Few biomarkers are available for predicting chemotherapeutic response and prognosis in colorectal cancer (CRC). Long-noncoding RNAs (lncRNAs) are essential for CRC development and growth. Therefore, studying lncRNAs may reveal potential predictors of chemotherapy response and prognosis in CRC.

View Article and Find Full Text PDF
Article Synopsis
  • The study investigates risks of bleeding and ischemia in elderly patients (≥65 years) with acute coronary syndrome and atrial fibrillation, highlighting the lack of specific prognostic models for this demographic.
  • A cohort of 1,851 patients across multiple hospitals in China was analyzed over a year, leading to the development of two effective prognostic models for predicting bleeding and ischemic events, which showed strong performance in both internal and external validation cohorts.
  • The study provides online calculators based on these models to aid healthcare professionals in personalized risk assessment and decision-making for elderly patients facing these common cardiovascular issues.
View Article and Find Full Text PDF

Inspired by the concept of "natural camouflage," biomimetic drug delivery systems have emerged to address the limitations of traditional synthetic nanocarriers, such as poor targeting, susceptibility to identification and clearance, inadequate biocompatibility, low permeability, and systemic toxicity. Biomimetic nanocarriers retain the proteins, nucleic acids, and other components of the parent cells. They not only facilitate drug delivery but also serve as communication media to inhibit tumor cells.

View Article and Find Full Text PDF

Comparative assessment of acute neurotoxicity of real-world ultra-fine black carbon emitted from residential solid fuel combustion.

Sci Total Environ

December 2024

State Key Joint Laboratory for Environmental Simulation and Pollution Control, State Environmental Protection Key Laboratory of Atmospheric Exposure and Health Risk Management, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.

Article Synopsis
  • Incomplete combustion of residential solid fuels is a major source of black carbon (BC), which can be harmful to brain health due to its ability to cross the blood-brain barrier.
  • The study found that straw-derived BC had the highest neurotoxic effects on human neuroblastoma cells, while coal-derived BC showed minimal toxicity even at high concentrations.
  • Various biological mechanisms were identified, with biomass BC impacting mitochondrial function and metabolic processes, while coal BC primarily triggered inflammatory responses through specific signaling pathways.
View Article and Find Full Text PDF

Liver mechanosignaling as a natural anti-hepatitis B virus mechanism.

Nat Commun

September 2024

Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Huashan Hospital, Shanghai Medical College Fudan University, Shanghai, China.

The mechanisms underlying the natural control of hepatitis B virus (HBV) infection have long been an intriguing question. Given the wide physiological range of liver stiffness and the growing attention to the role of mechanical microenvironment in homeostasis and diseases, we investigated how physical matrix cues impact HBV replication. High matrix stiffness significantly inhibited HBV replication and activated YAP in primary hepatocyte culture system, a key molecule in mechanosignaling.

View Article and Find Full Text PDF

The extracellular matrix (ECM) plays a crucial role in maintaining cell morphology and facilitating intercellular signal transmission within the human body. ECM has been extensively utilized for tissue injury repair. However, the consideration of factor gradients during ECM preparation has been limited.

View Article and Find Full Text PDF
Article Synopsis
  • lncRNA, specifically HIF1A-AS2, is found to be upregulated in colorectal cancer (CRC) tissues, linking it to poor patient outcomes and tumor development.
  • HIF1A-AS2 promotes cancer cell proliferation, metastasis, and a shift to aerobic glycolysis by influencing the expression of FOXC1 through direct interaction with miR-141-3p.
  • The study suggests that HIF1A-AS2, regulated by SP1 and also packaged in exosomes, could serve as a valuable diagnostic marker and a potential target for CRC treatments.
View Article and Find Full Text PDF

Pyroptosis may play an important role in the resistance of ovarian cancer (OC) to chemotherapy. However, the mechanism by which pyroptosis modulation can attenuate chemotherapy resistance has not been comprehensively studied in OC. Here, we demonstrated that RAS-associated C3 botulinum toxin substrate 1 (RAC1) is highly expressed in OC and is negatively correlated with patient outcomes.

View Article and Find Full Text PDF

Background: In relapsed/refractory (R/R) diffuse large B-cell lymphoma (DLBCL), a negative prognosis is frequently linked to heightened epigenetic heterogeneity. Chidamide, a selective histone deacetylase inhibitor, shows promise as a targeted therapy for R/R DLBCL by targeting abnormal epigenetic changes associated with poor prognosis.

Methods: A cohort of 27 ineligible patients with R/R DLBCL participated in an open - label, single - arm study.

View Article and Find Full Text PDF

Understanding the global cancer statistics 2022: growing cancer burden.

Sci China Life Sci

October 2024

Department of Thoracic Surgery, Department of International MedicalServices, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, 100730, China.

View Article and Find Full Text PDF

Introduction: The apolipoprotein E (APOE) ε4 allele exerts a significant influence on peripheral inflammation and neuroinflammation, yet the underlying mechanisms remain elusive.

Methods: The present study enrolled 54 patients diagnosed with late-onset Alzheimer's disease (AD; including 28 APOE ε4 carriers and 26 non-carriers). Plasma inflammatory cytokine concentration was assessed, alongside bulk RNA sequencing (RNA-seq) and single-cell RNA sequencing (scRNA-seq) analysis of peripheral blood mononuclear cells (PBMCs).

View Article and Find Full Text PDF

Background: ATP binding cassette transporter A1 (ABCA1) is a candidate gene within a POAG susceptibility locus by GWAS analysis, and it is involved in IOP modulation via the Cav1/eNOS/NO signaling pathway. We aim to examine the phenotype of ABCA1 deletion in the ABCA1 gene knockout (Abca1) mice.

Methods: The anterior segments of Abca1 eyes were imaged by slit-lamp microscopy and anterior segment OCT.

View Article and Find Full Text PDF

The etiology of gastrointestinal (GI) diseases is intricate and multifactorial, encompassing complex interactions between genetic predisposition and gut microbiota. The cell fate change, immune function regulation, and microenvironment composition in diseased tissues are governed by microorganisms and mutated genes either independently or through synergistic interactions. A comprehensive understanding of GI disease etiology is imperative for developing precise prevention and treatment strategies.

View Article and Find Full Text PDF

Urinary extracellular vesicles (uEVs) are rich in valuable biomolecule information which are increasingly recognized as potential biomarkers for various diseases. uEV long RNAs are among the critical cargos capable of providing unique transcriptome information of the source cells. However, consensus regarding ideal reference genes for relative long RNAs quantification in uEVs is not available as of date.

View Article and Find Full Text PDF

Modulation of monocyte activity by hepatocellular MicroRNA delivery through HBsAg particles: Implications for pathobiology of chronic hepatitis B.

Hepatology

June 2024

Key Laboratory of Medical Molecular Virology (MOE/NHC), Research Unit of Cure of Chronic Hepatitis B Virus Infection (CAMS), Shanghai Frontiers Science Center of Pathogenic Microbes and Infection, School of Basic Medical Sciences, Shanghai Medical College Fudan University, Shanghai, China.

Background And Aims: HBsAg serves as an important immune-modulatory factor in chronic hepatitis B. One aspect of such modulation may act through monocytes, which are the major Ag-presenting cells taking up HBsAg. There is evidence for the encapsulation of hepatocellular microRNAs (miRNAs) by HBsAg particles, while its pathobiological significance is unclear.

View Article and Find Full Text PDF
Article Synopsis
  • This study collected data from over 35,000 COVID-19 patients in China to analyze the rates of olfactory (smell) and gustatory (taste) dysfunctions following infection.!
  • Findings revealed a 67.75% incidence rate of these dysfunctions, with factors like gender, smoking, vaccination doses, and oral health affecting both the likelihood of experiencing and recovering from these symptoms.!
  • On average, smell and taste scores declined significantly after infection but showed some recovery over time, with 59.16% of patients reporting improvement, though some experienced prolonged symptoms lasting over 28 days.!
View Article and Find Full Text PDF

Association of Human Whole-blood NAD Levels with Nabothian Cyst.

Biomed Environ Sci

May 2024

Department of Neurosurgery, Neurosurgery Research Institute, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350005, Fujian, China;Department of Neurosurgery, Binhai Branch of National Regional Medical Center, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350209, Fujian, China;Fujian Provincial Institutes of Brain Disorders and Brain Sciences, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350005, Fujian, China;Fujian Provincial Clinical Research Center for Neurological Diseases, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350005, Fujian, China.

Article Synopsis
  • * Analysis showed that nabothian cyst prevalence decreased as NAD levels increased, with the highest prevalence observed in the lowest NAD quartile.
  • * Specifically, participants in the lowest NAD quartile had a significantly higher adjusted odds ratio for having total nabothian cysts compared to those with the highest NAD levels, indicating that higher NAD levels may reduce the risk of these cysts.
View Article and Find Full Text PDF

Hepatitis B Virus (HBV) infection is a global public health challenge that seriously endangers human health. Soft coral, as a major source of terpenoids, contains many structurally novel and highly bioactive compounds. Sixteen cembranoids (1-16), including a new one named sinupedunol B (16), were isolated from the South China Sea Soft coral Sinularia pedunculata.

View Article and Find Full Text PDF

Background/aims: Ulcerative colitis (UC) is an incurable, relapsing-remitting inflammatory disease that increases steadily. Mucosal healing has become the primary therapeutic objective for UC. Nevertheless, endoscopic assessments are invasive, expensive, time-consuming, and inconvenient.

View Article and Find Full Text PDF