Osteopontin Facilitated Dental Pulp Cell Adhesion and Differentiation: A Laboratory Investigation.

Aim: To investigate the effects of osteopontin (OPN) on cultured human dental pulp cells (hDPCs) in relation to adhesion, proliferation, differentiation, and mineralization.

Methodology: Subcultured hDPCs isolated from healthy human wisdom teeth were inoculated on noncoated (NC, control) and OPN-coated nontissue culture-treated polystyrene plates (Non-TCPS). Cell adhesion and proliferation were analyzed by crystal violet staining and the CCK-8 assay, respectively.

Aneurysmal Bone Cyst of the Jaws: A Retrospective Analysis of 19 Cases.

Objective: To analyse the clinical, radiographic, and pathological characteristics of aneurysmal bone cyst of the jaws (JABCs), and to provide reference for clinical diagnosis and treatment.

Materials And Methods: A retrospective study was conducted on 19 patients with JABC diagnosed from 2018 to 2022.

Results: Of the 19 JABC patients (5 males, 14 females, aged 7-53 years, mean 23.

Adaptation of maxillary removable partial denture frameworks fabricated with a direct digital workflow: A randomized crossover clinical trial.

Objectives: To compare the adaptation of maxillary removable partial denture (RPD) frameworks fabricated through direct digital workflows with that of traditional cast frameworks and indirect digital frameworks.

Methods: The workflow for fabricating the digital cobalt-chromium framework encompassed intraoral scanning (IOS) using Trios 3, computer-aided survey and design, and subsequently either the lost-wax technique from a printed resin framework pattern (Framework B) or direct selective laser melting (SLM) (Framework C). The traditional cast framework (Framework A) was selected as a control.

Quantitative Evaluation of Multiple Treatment Regimens for Treatment-Resistant Depression.

Objective: This study aims to quantitatively evaluate the efficacy and safety of various treatment regimens for treatment-resistant depression (TRD) across oral, intravenous, and intranasal routes to inform clinical guidelines.

Methods: A systematic review identified randomized controlled trials on TRD, with efficacy measured by changes in the Montgomery-Åsberg Depression Rating Scale (MADRS). We developed pharmacodynamic and covariate models for different administration routes, using Monte Carlo simulations to estimate efficacy distribution.

[Frameshift mutation in gene causes amelogenesis imperfecta].

Objective: To analyze gene mutation found in a pedigree with clinical features and inheritable pattern consistent with amelogenesis imperfecta (AI) in China, and to study the relationship between its genotype and phenotype.

Methods: Clinical and radiological features were recorded for the affected individuals. Peripheral venous blood samples of the patient and family members were collected for further study, and the genomic DNA was extracted to identify the pathogenic gene.

Enhancing Diabetic Oral Wound Healing with miR-132 Delivered Through Tetrahedral DNA Nanostructures.

Oral mucosal injuries are commonly caused by factors such as trauma, infection, or inflammation, especially in diabetic patients where healing is difficult and significantly affects quality of life. In this study, a nanocarrier system based on DNA tetrahedrons (TDN) is developed, which serve as ideal vectors due to their excellent intracellular uptake and drug delivery capabilities. By efficiently delivering miR132 into cells, the proliferation and migration of human oral mucosal fibroblasts (HOMFs) and human umbilical vein endothelial cells (HUVECs) are regulated, along with the modulation of inflammation and antioxidant processes.

Biosynthesis of Lysosomally Escaped Apoptotic Bodies Inhibits Inflammasome Synthesis in Macrophages.

Hyperglycemia and bacterial colonization in diabetic wounds aberrantly activate Nod-like receptor protein 3 (NLRP3) in macrophages, resulting in extensive inflammatory infiltration and impaired wound healing. Targeted suppression of the NLRP3 inflammasome shows promise in reducing macrophage inflammatory disruptions. However, challenges such as drug off-target effects and degradation via lysosomal capture remain during treatment.

Photodynamic and photothermal bacteria targeting nanosystems for synergistically combating bacteria and biofilms.

The escalating hazards posed by bacterial infections underscore the imperative for pioneering advancements in next-generation antibacterial modalities and treatments. Present therapeutic methodologies are frequently impeded by the constraints of insufficient biofilm infiltration and the absence of precision in pathogen-specific targeting. In this current study, we have used chlorin e6 (Ce6), zeolitic imidazolate framework-8 (ZIF-8), polydopamine (PDA), and UBI peptide to formulate an innovative nanosystem meticulously engineered to confront bacterial infections and effectually dismantle biofilm architectures through the concerted mechanism of photodynamic therapy (PDT)/photothermal therapy (PTT) therapies, including in-depth research, especially for oral bacteria and oral biofilm.

Colored Cellulose Nanoparticles with High Stability and Easily Modified Surface for Accurate and Sensitive Multiplex Lateral Flow Assay.

Decentralized testing using multiplex lateral flow assays (mLFAs) to simultaneously detect multiple analytes can significantly enhance detection efficiency, reduce cost and time, and improve analytic accuracy. However, the challenges, including the monochromatic color of probe particles, interference between different test lines, and reduced specificity and sensitivity, severely hinder mLFAs from wide use. In this study, we prepared polydopamine (PDA)-coated dyed cellulose nanoparticles (dCNPs@P) with tunable colors as the probe for mLFAs.

Tetrahedral Framework Nucleic Acid Relieves Sepsis-Induced Intestinal Injury by Regulating M2 Macrophages.

This study aimed to clarify the role and mechanism of tetrahedral framework nucleic acids (tFNAs) in regulating M2 macrophages to reduce intestinal injury. An intestinal injury model was established by intraperitoneal injection of lipopolysaccharides (LPS) in mice to explore the alleviating effects of tFNAs on intestinal injury. Inflammatory factors were detected by quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA).

Mechanism of hyperbaric oxygen therapy downregulating H-type angiogenesis in subchondral bone of knee osteoarthritis through the PHD2/HIF-1α pathway.

Objective: To explore the mechanism of hyperbaric oxygen therapy in inhibiting subchondral bone angiogenesis and delaying the progression of osteoarthritis through the PHD2/HIF-1α signaling pathway.

Methods: Mice were randomly divided into three groups (control group, osteoarthritis group, and hyperbaric oxygen treatment group). The effect of hyperbaric oxygen therapy on osteoarthritis was evaluated using Micro-CT, Safranin O-Fast Green staining, and detection of osteoarthritis inflammation markers (MMP-13, ADAMTS-5, Col2a1, and Aggrecan).

PRMT1-methylated MSX1 phase separates to control palate development.

Little is known about the regulation and function of phase separation in craniofacial developmental disorders. MSX1 mutations are associated with human cleft palate, the most common craniofacial birth defect. Here, we show that MSX1 phase separation is a vertebrate-conserved mechanism underlying embryonic palatal fusion.

Click chemistry-driven asymmetrically modified superhydrophobic/superhydrophilic gelatin sponge for improved dry socket prophylaxis.

Dry socket, a common painful complication after tooth extraction, is typically caused by improper blood clot formation or its premature dislodgement, often exacerbated by bacterial infections. Traditional gelatin sponges, widely used as clinical fillers, provide favorable biocompatibility and hemostatic support but suffer from suboptimal hemostatic efficiency, lack of antimicrobial properties, and insufficient anticoagulant factors, which increase the risk of dry socket. Addressing these limitations, a novel tannic acid cross-linked gelatin sponge has been developed using directional lyophilization.

BTG3 inhibits porcine epidemic diarrhea virus replication by promoting viral S2 protein degradation through the autophagy and proteasome pathways.

BTG3, which belongs to the BTG/Tob gene family, is involved in various physiological processes. Infection with porcine epidemic diarrhea virus (PEDV), an alphacoronavirus, is associated with high mortality rates among piglets, contributing to major economic losses. This study elucidated a novel mechanism through which BTG3 suppresses PEDV replication.

Metabolic Reprogramming of Neural Stem Cells by Chiral Nanofiber for Spinal Cord Injury.

Exogenous neural stem cells (NSCs) have great potential to reconstitute damage spinal neural circuitry. However, regulating the metabolic reprogramming of NSCs for reliable nerve regeneration has been challenging. This report discusses the biomimetic dextral hydrogel (DH) with right-handed nanofibers that specifically reprograms the lipid metabolism of NSCs, promoting their neural differentiation and rapid regeneration of damaged axons.

Expert consensus on classification and diagnosis of congenital orofacial cleft.

Congenital orofacial cleft, the most common birth defect in the maxillofacial region, exhibits a wide range of prognosis depending on the severity of deformity and underlying etiology. Non-syndromic congenital orofacial clefts typically present with milder deformities and more favorable treatment outcomes, whereas syndromic congenital orofacial clefts often manifest with concomitant organ abnormalities, which pose greater challenges for treatment and result in poorer prognosis. This consensus provides an elaborate classification system for varying degrees of orofacial clefts along with corresponding diagnostic and therapeutic guidelines.

Huoxiang Zhengqi dropping pills alleviate exertional heat stroke-induced multiple organ injury through sustaining intestinal homeostasis via regulating MAPK/NF-κB pathway and gut microbiota in rats.

Exertional heat stroke (EHS) is a life-threatening condition characterized by hyperthermia and multi-organ dysfunction, often associated with intestinal barrier disruption. This study evaluated the protective effects of Huoxiang Zhengqi Dropping Pills (HXZQD) against EHS in a rat model. HXZQD was administered via oral gavage at low, medium, and high doses, followed by EHS induction through exercise under high-temperature and high-humidity conditions.

GelMA Hydrogels Integrated With aptamer CH6-Functionalized Tetrahedral DNA Nanostructures for Osteoporotic Mandibular Regeneration.

Osteoporotic bone regeneration is challenging due to impaired bone formation. Tetrahedral DNA nanostructures (TDN), promising nucleic acid nanomaterials, have garnered attention for their potential in osteoporotic mandibular regeneration owing to their ability to enhance cellular activity and promote osteogenic differentiation. Osteoblasts play a critical role in bone regeneration; however, intracellular delivery of TDN into osteoblasts remains difficult.

Predicting dengue incidence in high-risk areas of China through the integration of Southeast Asian and local meteorological factors.

Dengue, a climate-sensitive mosquito-borne viral disease, is endemic in many tropical and subtropical areas, with Southeast Asia bearing the highest burden. In China, dengue epidemics are primarily influenced by imported cases from Southeast Asia. By integrating monthly maximum temperature and precipitation from Southeast Asia and local provinces in China, we aim to build models to predict dengue incidence in high-risk areas of China.

Dedicator of Cytokinesis 2 regulates cytoskeletal actin dynamics and is essential for platelet biogenesis and functions.

Aims: Dedicator of Cytokinesis 2 (DOCK2), a member of the DOCK family of Guanine nucleotide exchange factors that specifically act on the Rho GTPases including Rac and Cdc42, plays pivotal roles in the regulation of leukocyte homeostasis. However, its functions in platelets remain unknown.

Methods And Results: Using mice with genetic deficiency of DOCK2 (Dock2-/-), we showed that Dock2-/-mice exhibited a macrothrombocytopenic phenotype characterized as decreased platelet count and enlarged platelet size by transmission electron microscopy.

LIMA1 Is a Prognostic Senescence-Inhibitory Gene in Head and Neck Squamous Carcinoma.

Background: This study aimed to investigate potential cellular senescence inhibitory genes (CSIGs) and discover novel therapeutic targets in head and neck squamous cell carcinoma.

Methods: Dysregulated CSIGs were identified based on The Cancer Genome Atlas (TCGA) and the Human Aging Genomic Resources (HAGR) database. Prognostic value and immune infiltration were assessed through bioinformatic analysis.

Gut bacterium Intestinimonas butyriciproducens improves host metabolic health: evidence from cohort and animal intervention studies.

Background: The human gut microbiome strongly influences host metabolism by fermenting dietary components into metabolites that signal to the host. Our previous work has shown that Intestinimonas butyriciproducens is a prevalent commensal bacterium with the unique ability to convert dietary fructoselysine to butyrate, a well-known signaling molecule with proven health benefits. Dietary fructoselysine is an abundant Amadori product formed in foods during thermal treatment and is part of foods rich in dietary advanced glycation end products which have been associated with cardiometabolic disease.

A Population-Based Study of the Association Among Dry Mouth, Vitamin B2, and Mortality.

Background: Previous studies have indicated that older adults with the symptom of dry mouth have an increased risk of death. B vitamins are strongly linked to oral and systemic health. However, the relationship among dry mouth, vitamin B2 and mortality remains unclear.

Tetrahedral Framework Nucleic Acid (tFNA)-Loaded Metformin (Met) Modulates Cellular Pyroptosis and AMPK to Ameliorate Type II Diabetic Periodontitis.

Diabetic periodontitis presents a unique therapeutic challenge, primarily due to its chronic inflammatory profile and the associated bone loss driven by immune dysregulation. Metformin (Met) is recognized for its potent anti-inflammatory properties, yet its limited cellular uptake has hindered its clinical effectiveness in diabetic periodontitis. A tetrahedral framework nucleic acid (tFNA)-based delivery system is developed to enhance Met cellular uptake and investigate its effects on diabetic periodontitis in this study.