Enhancement of CD8T cell cytotoxicity activity by IFN-α implies alternative pathologic role in systemic lupus erythematosus.

Objective: Systemic lupus erythematosus (SLE) is a heterogeneous autoimmune disease which is affected by the environmental, genetic factors as well as the immune system. Previous reports have implicated IFN-α in the pathogenesis of SLE. Up to date, however, no research has ever investigated the effect of IFN-α on CD8T cells, which might be implicated in the pathogenesis of SLE.

The effect of camera side on the VOR gain in video head impulse test.

Objective: This study aims to investigate the effect of camera positioning (left-sided vs right-sided) on the vestibulo-ocular reflex (VOR) gain values of normal subjects during the video head impulse test (vHIT), thereby providing valuable reference data for otology clinical practice.

Methods: Thirty healthy adults (60 ears) were recruited and examined by vHIT for the three pairs of semicircular canals (SCs). During the examination, we meticulously recorded and compared the VOR gain values in the six canal planes-right lateral (RL), left lateral (LL), right anterior-left posterior (RALP), and left anterior-right posterior (LARP)-with the camera positioned on the left side first, followed by repositioning on the right side.

Distinct immunophenotypic profiles and neutrophil heterogeneity in colorectal cancer.

Colorectal cancer (CRC) exhibits significant molecular and immunological heterogeneity. Neutrophil infiltration patterns play a crucial yet poorly understood role in tumor progression and patient outcomes. This study presents a comprehensive single-cell atlas of the CRC tumor microenvironment (TME), integrating transcriptomic data from 388,511 cells across 98 samples from 63 patients.

Target Achievements of Low-Density Lipoprotein Cholesterol, Blood Pressure, and Glucose in Patients with Diabetes after Acute Coronary Syndrome: Findings from the Chinese Cardiovascular Association Database - iHeart Project.

Aim: To evaluate the achievement of metabolic risk factor targets and influencing factors in ACS patients with diabetes during the 12 months after discharge.

Methods: We retrospectively analyzed data from the Chinese Cardiovascular Association database-iHeart Project. Patients who were hospitalized with a diagnosis of ACS between 2014 and 2021 and who had at least one measurement record of LDL-C, BP, or HbA within 12 months after discharge were included.

Synergistic Enhancement of Therapeutic Efficacy in Acute Myocardial Infarction via Nanoflower-Like MnO Nanozymes in Coordination with Adipose-Derived Stem Cell Transplantation.

Background: Acute myocardial infarction (AMI) is a leading cause of mortality worldwide. Adipose-derived stem cell (ADSC) transplantation presents a promising therapeutic approach for AMI; however, the harsh microenvironment of the infarcted myocardium, characterized by hypoxia and oxidative stress, limits the survival and efficacy of ADSCs. Nanozymes (NZs), which have robust anti-oxidative enzyme-mimicking activities, have demonstrated potential in combating oxidative stress and improving cell viability.

CD34 PI16 fibroblast progenitors aggravate neointimal lesions of allograft arteries via CCL11/CCR3-PI3K/AKT pathway.

Transplant-accelerated arteriosclerosis is a common complication that limits the long-term survival of organ transplant recipients. While previous studies have indicated the involvement of CD34 stem/progenitor cells (SPCs) in this process, their heterogeneity and potential adverse effects remains incompletely understood. To investigate the role of CD34 SPCs in transplant arteriosclerosis, we used various genetically modified mouse models, including BALB/c, C57BL/6J, CD34-CreER, Rosa26-tdTomato, Rosa26-iDTR, CD34-Dre, PI16-CreER, and CAG-LSL-RSR-tdTomato-2A-DTR mice.

Identification of new candidate drugs in myelodysplastic syndromes with splicing factor mutations by transcriptional profiling and connectivity map analysis.

We sought to identify new candidate drugs for repurposing to myelodysplastic syndromes (MDS). Connectivity map analysis was performed on gene expression signatures generated from bone marrow CD34 cells of splicing factor mutant MDS patients. Celastrol and Withaferin A (WA), two top-ranking compounds identified, markedly inhibited proliferation, arrested the cell cycle and induced apoptosis in leukaemia cells.

Structural and functional stenosis of the upper airway in Crouzon syndrome patients: A computational fluid dynamics analysis.

Objectives: This study aimed to simulate the aerodynamics and to identify the spatial correlation between anatomical and functional stenoses in Crouzon syndrome patients.

Methods: Six patients of Crouzon syndrome were included. Computational fluid dynamics (CFD) was utilized to simulate airflow dynamics, and characteristics, including the velocity, pressure intensity, wall shear stress, airflow resistance and streamline, were extracted for quantitative analysis both in overall and regionally.

Systematic molecular profiling of non-native N-substitution effects on m6A binding to the YTH domains of human RNA m6A readers in diabetes.

The RNA N-adenosine methylation, resulting in N-methyl adenosine (m6A), is one of the most important post-transcriptional modification events in the eukaryotic transcriptome, which is dynamically regulated by methyltransferases (writers), recognition proteins (readers) and demethylases (erasers). Human has five m6A readers namely YTHDC1, YTHDC2, YTHDF1, YTHDF2 and YTHDF3 that specifically recognize and bind to the methylated m6A residue of RNA through their YT521-B homology (YTH) domains, which have been involved in the pathogenesis of diabetes mellitus and its diverse complications such as diabetic nephropathy. Instead of the native N-methylation, we herein attempted to explore the molecular effect of various non-native N-substitutions on adenosine (A) binding behavior to YTH domains.

Genetic insights into circulating osteocalcin for cardiovascular diseases and the role of vascular calcification.

Background And Aims: Studies have suggested that osteocalcin (OCN) is implicated in vascular calcification and linked to cardiovascular diseases (CVDs), but it is unclear whether the relationships are causal. The aim of this study is to evaluate the causal relationship of circulating OCN with CVDs and the role of vascular calcification.

Methods And Results: Bi-directional, mediation, and multivariable Mendelian randomization (MVMR) were performed using summary-level data for circulating OCN levels, coronary artery calcification (CAC), and CVDs, including coronary artery disease (CAD), myocardial infarction (MI), heart failure, atrial fibrillation, stroke and its subtypes.

Baicalin alleviates lipid metabolism disorders in diabetic kidney disease via targeting FKBP51.

Background: Diabetic kidney disease (DKD) represents the primary aetiological factor in end-stage renal disease, wherein lipid metabolism disorders contribute to the progression of DKD. Baicalin, a composition from Scutellaria baicalensis, has exhibited the potential to mitigate lipid metabolism disorders of DKD, but the precise mechanisms remain unclear.

Methods: High-fat-diet (HFD)/streptozotocin (STZ)-induced DKD mouse model was established to appraise the effects of baicalin on renal function, dyslipidemia, and renal ectopic lipid deposition.

Sirolimus- vs Paclitaxel-Coated Balloon for the Treatment of Coronary In-Stent Restenosis: The SIBLINT-ISR Randomized Trial.

Background: The use of drug-coated balloons is a well-established strategy for the management of coronary vessels. However, head-to-head comparisons of sirolimus-coated balloons (SCBs) and paclitaxel-coated balloons (PCBs) for treating in-stent restenosis (ISR) are currently limited.

Objectives: The aim of this randomized, controlled trial (SIBLINT-ISR [Sirolimus-Coated Balloon Versus Paclitaxel-Coated Balloon for the Treatment of Coronary In-Stent Restenosis]) was to compare a novel SCB with a PCB for the treatment of ISR.

Addressing challenges in pediatric thrombosis: a comprehensive guideline development.

Background: Pediatric thrombosis is a relatively rare but severe condition in the field of pediatrics, with far-reaching consequences. Recent studies have indicated a rising incidence of this disease in children over the years. Additionally, the pharmacological treatment of thrombotic diseases in children faces numerous challenges.

Allostatic load in non-medical and medical college students.

Background: Physical ailments and mental illness among college students are surging in recent years, which warrants urgent and comprehensive health research.

Objective: To investigate whether there is a disparity in allostatic load between medical and non-medical college students, and to identify the influencing factors on allostatic load.

Methods: A cross-sectional study involving 8,253 college students was conducted.

An ultrasound-guided modified iliac fascia and sacral plexus block application in a critically ill patient undergoing artificial femoral head replacement surgery: a case report.

Background: Hip fractures have become a significant clinical concern on a global scale in recent years. The burgeoning aging population has exacerbated this issue, leading to a rise in the number of hip fracture cases coupled with concomitant geriatric ailments. Therefore, it poses a huge challenge to anesthesiologists with the increasing number of critically ill patients who are not suitable for general anesthesia and intrathecal anesthesia.

mGluR5-mediated astrocytes hyperactivity in the anterior cingulate cortex contributes to neuropathic pain in male mice.

Astrocytes regulate synaptic transmission in healthy and pathological conditions, but their involvement in modulating synaptic transmission in chronic pain is unknown. Our study demonstrates that astrocytes in the anterior cingulate cortex (ACC) exhibit abnormal calcium signals and induce the release of glutamate in male mice. This leads to an elevation in extracellular glutamate concentration, activation of presynaptic kainate receptors, and an increase in synaptic transmission following neuropathic pain.

Elevated expression of Piezo1 activates the cGAS-STING pathway in chondrocytes by releasing mitochondrial DNA.

Objective: Abnormal mechanical stress is a key factor in osteoarthritis (OA) pathogenesis. This study aims to investigate the role of the mechanosensitive ion channel Piezo1 in activating the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway and its contribution to cartilage degradation in OA.

Methods: We conducted both in vivo and in vitro experiments.

Artificial intelligence algorithm for preoperative prediction of FIGO stage in ovarian cancer based on clinical features integrated 18F-FDG PET/CT metabolic and radiomics features.

Purpose: The International Federation of Gynecology and Obstetric (FIGO) stage is critical to guiding the treatments of ovarian cancer (OC). We tried to develop a model to predict the FIGO stage of OC through machine learning algorithms with patients' pretreatment clinical, positron emission tomography scan (PET/CT) metabolic, and radiomics features.

Methods: We enrolled OC patients who underwent PET/CT scans and divided them into two cohorts according to their FIGO stage.

Understanding Caregiver Burden in Severe Pediatric Asthma - A Qualitative Study.

Objective: This study aimed to clarify the emotional and practical burden on primary caregivers of children with severe asthma and identify the support needs of those caring for individuals with severe asthma.

Methods: A phenomenological research approach in qualitative research was used. Semi-structured interviews with caregivers of children with asthma waiting in the outpatient clinic of Shanghai Children's Hospital were conducted from January to August 2024 through purposive sampling.

Decoding CKD-induced muscle atrophy through the critical role of lncRNA GAS5 and pyroptosis.

Skeletal muscle atrophy is a prevalent complication of chronic kidney disease (CKD) and serves as an indicator of adverse prognosis and poor quality of life; however, the underlying mechanisms remain ambiguous. Emerging evidence has shown that long non-coding RNAs (lncRNAs) are involved in the pathogenesis of skeletal muscle atrophy. Using RNA sequencing (RNA-seq), we discerned elevated GAS5 expression in the muscles of CKD mice and verified these findings by real-time qPCR.

Association of body roundness index with cardiovascular disease and all-cause mortality among Chinese adults.

Aims: To determine the impact of body roundness index (BRI) on the 10-year risk of cardiovascular disease (CVD) and all-cause mortality in Chinese adults.

Materials And Methods: We utilized data from a nationwide prospective cohort of 165 785 Chinese adults (aged ≥40 years, free of CVD and cancer at baseline), which was extracted from the China Cardiometabolic Disease and Cancer Cohort (4C). Cox regression and restricted cubic splines quantified BRI-outcome associations and identified optimal BRI cutoffs.

[The opportunities and challenges brought by the age of we media].

In recent years, surgical research has entered the digital era, and the dissemination of medical knowledge has also begun to confront the challenge posed by we media. When we media first emerged, most doctors in public hospitals, who focused on "medicine, education, and research", held a reserved attitude towards it. However, we media has demonstrated remarkable resilience and has continuously evolved to align with the trend of social development.

Description of physical activity and screen time among U.S. middle school students.

Background: Physical activity (PA) is important for students in secondary school, however, trends in PA among secondary school students have shown a significant decline. There is a need to understand the PA of middle school students.

Objective: The first objective is to identify the PA levels and screen time of students in middle school.

Valsartan to Prevent Acquired Pulmonary Vein Stenosis in Pediatric Patients After Total Anomalous Pulmonary Venous Connection Surgery.

Background: Recurrent pulmonary vein stenosis (PVS) following surgical repair of total anomalous pulmonary venous connection is associated with poor prognosis. Preclinical studies have shown that use of an angiotensin receptor blocker can attenuate intimal hyperplasia; notwithstanding, its clinical utility is of uncertain benefit.

Methods: This single-center study included patients undergoing total anomalous pulmonary venous connection repair in 2020 to 2021.