A spherical poly(acrylic acid) brush-enzyme block with high catalytic capacity for signal amplification in digital biological assays.

Ultrasensitive determination of some ultra-low abundance biological molecules closely related to diseases is currently a wide concern and urgent issue to be addressed. Here, a spherical poly(acrylic acid)-alkaline phosphatase (SP-AKP) signal amplification block using spherical poly(acrylic acid) brush nanoparticles (SP) as the immobilized carriers was designed and synthesized optimally first. The results show that a single SP-AKP with high enzyme binding capacity and high catalytic ability (up to about 4800 effective free AKP per SP-AKP) has much greater fluorescence signal amplification ability than a single free AKP or SiO-COOH-AKP.

HSC-specific knockdown of GGPPS alleviated CCl-induced chronic liver fibrosis through mediating RhoA/Rock pathway.

Hepatic stellate cells (HSCs) play a critical role in the pathogenesis and reversal of liver fibrosis. Targeting HSCs is of great significance in the treatment of hepatic fibrosis, and has attracted wide attention of scholars. Here we demonstrated that expression of geranylgeranyldiphosphate synthase (GGPPS) predominantly increased in HSCs in murine fibrotic liver.

Enhanced catalytic degradation of amoxicillin with TiO-FeO composites a submerged magnetic separation membrane photocatalytic reactor (SMSMPR).

A novel photo-Fenton catalytic system for the removal of organic pollutants was presented, including the use of photo-Fenton process and a submerged magnetic separation membrane photocatalytic reactor (SMSMPR). We synthesized TiO-FeO composites as the photocatalyst and made full use of the magnetism of the photocatalyst to realize the recollection of the catalyst from the medium, which is critical to the commercialization of photocatalytic technology for wastewater treatment. The photo-Fenton performance of TiO-FeO is evaluated with amoxicillin trihydrate (AMX) as a target pollutant.

High resolution tracking of macrophage cells in deep organs and lymphatics using fluorescent polymer dots.

cell tracking can provide information on cell migration and accumulation in the organs. Here, both folate and amino modified polymer dots were synthesized and screened for and tracking of macrophage Ana-1 cells. Flow cytometry analysis demonstrated that prepared polymer dots showed cellular uptake of approximately 98% within a short incubation time of 2 h, and these polymer dots maintained a cell labeling rate over 97% after 2 d.

Design of plasmonic nanomaterials for diagnostic spectrometry.

Molecular diagnostics relies on the efficient extraction of biomarker information from the given bio-systems. Plasmonic nanomaterials with tailored structural parameters are promising for the development of biomarker assays due to enrichment effect and signal enhancement. Herein, we overview the recent progress on the development of plasmonic nanomaterials for diagnostic spectrometry, encompassing the interface, mechanism, and application of these materials.

Proinflammatory and osteolysis-inducing effects of 3D printing Ti6Al4V particles and .

Ti6Al4V printing particles have been recently used for fabricating orthopedic implants. Removing these particles completely from fabricated implants is challenging. Furthermore, recycled particles are commonly used in fabrication without additional analysis.

Hyperoside inhibited the migration and invasion of lung cancer cells through the upregulation of PI3K/AKT and p38 MAPK pathways.

Non-small cell lung cancer (NSCLC) is one of the most common human malignancies. Malignant tumor is characterized by the Invasion and metastasis of tumor. Based on previous research, human A549 cells could be induced to apoptosis by Hyperoside (Hyp).

XBP1-LOX Axis is critical in ER stress-induced growth of lung adenocarcinoma in 3D culture.

Rapid growth of tumor cells needs to consume large amounts of oxygen and glucose, due to lack of blood supply within the tumor, cells live in an environment that lack of oxygen and nutrients. This environment results in endoplasmic reticulum (ER) stress and activates the UPR (unfolded protein response). More and more evidence suggests UPR provides a growth signal pathway required for tumor growth.

SIRT2 plays a significant role in maintaining the survival and energy metabolism of PIEC endothelial cells.

SIRT2, a member of the sirtuin (SIRT1-7) family, is a tubulin deacetylase. It has been reported that SIRT2 mediates cellular stress responses and is highly expressed in vascular endothelial cells, while its roles in cell survival and energy metabolism of endothelial cells remain unknown. In the current study, we tested our hypothesis that SIRT2 plays an important role in the cell survival and energy metabolism of endothelial cells, using a porcine vascular endothelial cell line (PIEC) as a cellular model.

Ischemia-induced Angiogenesis is Attenuated in Aged Rats.

To study whether focal angiogenesis is induced in aged rodents after permanent distal middle cerebral artery occlusion (MCAO), young adult (3-month-old) and aged (24-month-old) Fisher 344 rats underwent MCAO and sacrificed up to two months after MCAO. Immunohistochemistry and synchrotron radiation microangiography were performed to examine the number of newly formed blood vessels in both young adult and aged rats post-ischemia. We found that the number of capillaries and small arteries in aged brain was the same as young adult brain.

[Optimal sequence algorithm of divided period appointment consultation service sequence for re-visiting patients in the department of stomatology].

Purpose: An optimal sequence algorithm model of divided period appointment consultation service was built through optimal sequence algorithm.

Methods: Re-visiting patients in the department of stomatology in a tertiary hospital were enrolled as the research objects in this study. Information about the diagnose term of common diseases, tooth positions treated, doctor's consultation hours were collected.

Regulation of β-catenin-mediated esophageal cancer growth and invasion by miR-214.

The malignancy of esophageal cancer (EC) is largely due to its fast growth and invasion, in which WNT/β-catenin signaling plays a critical role. Hence, suppression of β-catenin signal transduction in EC cells may inhibit cancer growth and metastases. Among all microRNAs (miRNAs), miR-214 has been shown as a tumor suppressor in many cancers, but has yet studied in EC.

Inhibition of the growth of non-small cell lung cancer by miRNA-1271.

Non-small cell lung cancer (NSCLC) is a common lung cancer of high incidence. Since NSCLCs often grow rapidly, it is critical to elucidate the underlying mechanisms. Previous studies have shown that microRNAs (miRNAs) play critical roles in the carcinogenesis of NSCLC.

Expression of CCL2 is significantly different in five breast cancer genotypes and predicts patient outcome.

Breast cancer is one of the most common malignancies in women. Current treatment of breast cancer is mainly based on clinicopathological characteristics, and is not sufficiently customized for individual cases. The concept of genotyping in breast cancer was first proposed in 2001.

Role of WNT1-inducible-signaling pathway protein 1 in etoposide resistance in lung adenocarcinoma A549 cells.

Object: The aim of this study was to explore the role of WNT1-inducible-signaling Pathway Protein 1 (WISP-1) in etoposide resistance in lung adenocarcinoma A549 cells.

Methods: WISP-1 overexpression A549 lung adenocarcinoma cell was established. After exposure to ultraviolet (UV) and etoposide, cell viability and apoptosis were evaluated.

Caveolin-1 regulates cell apoptosis and invasion ability in paclitaxel-induced multidrug-resistant A549 lung cancer cells.

The aim of the study was to investigate the effect and potential mechanism of caveolin-1 (Cav1) knockdown in paclitaxel-resistant lung cancer A549/Taxol cells. The human paclitaxel-resistant lung cancer cell line A549/Taxol was transfected with a Cav1 shRNA lentiviral vector. Interference efficiency for Cav1 was detected by real-time PCR and Western blotting.

Aralar plays a significant role in maintaining the survival and mitochondrial membrane potential of BV2 microglia.

NADH shuttles mediate the transfer of the reducing equivalents of cytosolic NADH into mitochondria. Increasing evidence has suggested that malate-aspartate shuttle (MAS), one of the two types of NADH shuttles, plays important roles in certain biological processes. Aralar/AGC1, a Ca(2+)-dependent aspartate-glutamate carrier on mitochondrial membrane, is a component of MAS.

Nanoporous Metal Papers for Scalable Hierarchical Electrode.

are developed using Japanese washi paper as a template to create hierarchical porous electrodes. This method is used to create a trimodal -nanoporous Au electrode, as a well as a hierarchical NiMn electrode that achieves high electrochemical capacitance and a rapid rate of oxygen evolution.

On-Chip Micro-Pseudocapacitors for Ultrahigh Energy and Power Delivery.

based on hierarchical nanoporous hybrid electrodes consisting of 3D bicontinuous nanoporous gold and pseudocapacitive manganese oxide deliver an excellent stack capacitance of 99.1 F cm and a high energy density of 12.7 mW h cm with a retained high power density of 46.

XRCC1 genetic polymorphisms and sensitivity to platinum-based drugs in non-small cell lung cancer: an update meta-analysis based on 4708 subjects.

Objective: To evaluate the correlation between genetic polymorphisms in x-ray repair cross complementing group 1 (XRCC1) and sensitivity to platinum-based chemotherapy drugs in patients with non-small cell lung cancer.

Methods: Reports published before June 2014 were retrieved from the following databases: China Biology Medicine (CBM), China Academic Journal Full-Text Database (CNKI), China Science and Technology Journal Full-Text Database (VIP), Wanfang Data, PubMed and Excerpta Medica dataBASE (EMBASE). After extracting the data and evaluating the quality, meta-analysis was performed using RevMan5.

Formononetin suppresses the proliferation of human non-small cell lung cancer through induction of cell cycle arrest and apoptosis.

Formononetin is a novel herbal isoflavonoid isolated from Astragalus membranaceus and possesses antitumorigenic properties. In the present study, we investigated the anti-proliferative effects of formononetin on human non-small cell lung cancer (NSCLC), and further elucidated the molecular mechanism underlying the anti-tumor property. MTT assay showed that formononetin treatment significantly inhibited the proliferation of two NSCLC cell lines including A549 and NCI-H23 in a time- and dose-dependent manner.

Reduced E-Cadherin expression is a prognostic biomarker of non-small cell lung cancer: a meta-analysis based on 2395 subjects.

Objective: Previous studies related to the prognostic value of E-Cadherin expression on non-small cell lung cancer (NSCLC) were inconsistent. The present study aimed to evaluate the relation between E-Cadherin expression and the prognosis of NSCLC.

Methods: We performed a meta-analysis based on 14 studies including 2395 NSCLC patients.

Meta-analysis for curative effect of lobectomy and segmentectomy on non-small cell lung cancer.

Objective: To systematically evaluate 5-year survival, local recurrence rate, long-term recurrence rate and complications for lobectomy and segmentectomy in the treatment of NSCLC.

Methods: Articles on randomized and non-randomized clinical studies of 5-year survival, local recurrence, long-term recurrence and complications after lobectomy and segmentectomy for treatment of NSCLC.

Results: Among the included patients, no difference was found between the two groups in local recurrence.

SIRT2 plays a key role in both cell cycle regulation and cell survival of BV2 microglia.

Sirtuin 2 (SIRT2) is a member of sirtuin protein family. Previous studies have suggested that SIRT2 plays differential roles in the survival and cell cycle regulation of various cell types. Because microglia plays critical roles in multiple major neurological disorders, in our current study we investigated the roles of SIRT2 in regulation of the cell cycle and cell survival of BV2 microglia by applying SIRT2 siRNA.

Malate-aspartate shuttle mediates the intracellular ATP levels, antioxidation capacity and survival of differentiated PC12 cells.

NAD(+) and NADH play pivotal roles in numerous redox reactions in cells. While increasing evidence has indicated important roles of NAD(+) in cell survival and cellular functions, there has been distinct deficiency in the studies regarding the biological functions of NADH. NADH shuttles mediate the transfer of the reducing equivalents of the cytosolic NADH into mitochondria.