Molecular cloning and functional characterization of Schistosoma japonicum enolase which is highly expressed at the schistosomulum stage.

Enolase is a key enzyme in the glycolytic pathway; recent studies have also shown that enolase is found on the surface of several parasites, where it acts as a plasminogen-binding protein. In the present study, the enolase of Schistosoma japonicum has been cloned and expressed. In western blot analysis, the recombinant enolase from S.

Analysis of early hepatic stage schistosomula gene expression by subtractive expressed sequence tags library.

Schistosome parasites require a complex lifecycle requiring two hosts and aquatic phases of development. The schistosomula is a key phase of parasite development within the mammalian host, however relatively little is understood about the molecular processes underlying this stage. In this study 5723 subtractive expressed sequence tags (ESTs) were randomly selected from a 7 day hepatic schistosomula enriched library constructed using suppression subtractive hybridization method.