Serum Metabolic Fingerprints Characterize Systemic Lupus Erythematosus.

Metabolic fingerprints in serum characterize diverse diseases for diagnostics and biomarker discovery. The identification of systemic lupus erythematosus (SLE) by serum metabolic fingerprints (SMFs) will facilitate precision medicine in SLE in an early and designed manner. Here, a discovery cohort of 731 individuals including 357 SLE patients and 374 healthy controls (HCs), and a validation cohort of 184 individuals (SLE/HC, 91/93) are constructed.

RRM2 protects against ferroptosis and is a tumor biomarker for liver cancer.

Background: Ferroptosis is the process of cell death triggered by lipid peroxides, and inhibition of glutathione (GSH) synthesis leads to ferroptosis. Liver cancer progression is closely linked to ferroptosis suppression. However, the mechanism by which inhibition of GSH synthesis suppresses potential ferroptosis of liver cancer cells and whether ferroptosis-related liver cancer biomarkers have a promising diagnostic value remain unknown.

[Mechanism of Ferroptosis and Its Research Progress in Lung Cancer].

Ferroptosis is a recently recognized form of regulated cell death caused by an iron-dependent accumulation of lipid reactive species. However, little research on ferroptosis and lung cancer, one of the most common tumors, has been carried out. This paper tries to review the research progress of ferroptotic suppression and explain it from the different ways of ferroptosis occurrence.

O-GlcNAcylated c-Jun antagonizes ferroptosis via inhibiting GSH synthesis in liver cancer.

Ferroptosis is a metabolism-related cell death. Stimulating ferroptosis in liver cancer cells is a strategy to treat liver cancer. However, how to eradicate liver cancer cells through ferroptosis and the obstacles to inducing ferroptosis in liver cancer remain unclear.

Ferroptosis is governed by differential regulation of transcription in liver cancer.

Ferroptosis is an outcome of metabolic disorders and closely linked to liver cancer. However, the mechanism underlying the fine regulation of ferroptosis in liver cancer remains unclear. Here, we have identified two categories of genes: ferroptosis up-regulated factors (FUF) and ferroptosis down-regulated factors (FDF), which stimulate and suppress ferroptosis by affecting the synthesis of GSH.

[Implication of micrometastatic cancer cells in the peripheral blood on prognosis of non-small cell lung cancer].

Objective: To evaluate the relationship of micrometastatic cancer cells in the blood and prognosis of patients with non-small cell lung cancer (NSCLC).

Methods: Blood samples were collected from peripheral vein perioperatively and from the pulmonary vein intraoperatively in NSCLC patients. Cancer cells were detected by flow cytometry, as described previously.

Hematogenous dissemination of lung cancer cells during surgery: quantitative detection by flow cytometry and prognostic significance.

Shedding of neoplastic cells into the circulation is an essential event for the hematogenous metastasis of solid tumors. Recently, several studies reported that a high frequency of cancer cells could be detected in the bloodstream during surgery. The intraoperative detection of hematogenous dissemination of cancer cells was able to identify a subset of patients with malignant diseases at high risk for postoperative metastasis and to predict a poor prognosis.

[Vascular endothelial growth factor promotes hematogenous metastasis of cancer cells in patients with non-small cell lung cancer].

Objective: To evaluate the effect of vascular endothelia1 growth factor (VEGF) on the hematogenous metastasis of non-small cell lung cancer (NSCLC).

Methods: The identification of lung cancer cells in the peripheral blood were carried out by cytological, immunohistocytologica1 and immunofluorecent stains respectively, following isolation of cytokeratin-expressing cells with magnetic activated cell sorting. The quantification of cancer cells in the blood was performed according to the established flow cytometric assay.

[Analysis of circulating lung cancer cells in the peripheral blood in patients with lung cancer by flow cytometry].

Background: To analyze circulating lung cancer cells in the peripheral blood in patients with lung cancer by flow cytometry (FCM).

Methods: The monocyte fraction in peripheral blood was isolated by Ficoll-Hypaque gradient centrifugation. The cells obtained were labeled with antibodies against CD45, cytokeratin (CK) and antigen (2F7/S5A).

[Detection of telomerase activity in the blood of patients with lung cancer].

Background: To study the telomerase activity in blood cancer cells by TRAP for monitoring tumor metastasis in blood.

Methods: Twenty-five patients with lung cancer surgically treated and 35 patients before chemotherapy were determined for telomerase activity of cancer cells in the blood by TRAP, and 30 patients with non-tumor diseases as control.

Results: In the operative group,13 patients(52%) showed telomerase activity in pulmonary artery blood during operation, which was much higher than that of peripheral blood before operation (24%,P<0.

[An establishment of murine drug-resistant cell line transfected with human MDR-1 gene.].

Background: To investigate the relationship between MDR-1 gene expression and drug resistance in lung cancer cells.

Methods: The multidrug resistant cell line (3LL-MDR-1) was established by transfecting human MDR-1 gene expressing vector into a murine Lewis lung cancer cell line (3LL) . Drug resistance and the effect of valapamil (VLP) on the resistance were evaluated by MTT assay.