1 results match your criteria: "Shandong Stem Cell Engineering Research Center[Affiliation]"
[Expression and biology identification of the human epididymis-specific gene ESC42 in E. coli].
Zhonghua Nan Ke Xue
February 2005
Central Laboratory of Yantai Yuhuangding Hospital, Shandong Stem Cell Engineering Research Center, Yantai, Shandong 264000, China.
Objective: To provide materials for the study of the function of ESC42 protein specifically expressed in the human epididymis.
Methods: The ESC42 gene was amplified from the human epididymis cDNA library by PCR and then cloned into prokaryotic expression vector pGEX-4T-1, expressed and purified by recombinant DNA techniques. The specificity of ESC42 protein was identified by Western blot and MALDI-TOF-MS.