Platelet ultrastructural changes stored at room temperature versus cold storage observed by electron microscopy and structured illumination microscopy.

Our study seeks to provide a theoretical foundation for the clinical use of cold-stored platelets (CSPs) by interpreting ultrastructural images and quantitatively analyzing structural changes. CSPs, room temperature-stored platelets (RTPs), and delayed CSPs (delayed-CSPs) were continuously observed using scanning electron microscopy and transmission electron microscopy at eight time points. Super-resolution fluorescence microscopy was employed to observe changes in platelet microtubules and mitochondrial structure and function, whereas platelet counts, metabolism, and relevant functional indicators were measured concurrently.

SLC25A28 Overexpression Promotes Adipogenesis by Reducing ATGL.

Adipose tissue dysfunction is seen among obese and type 2 diabetic individuals. Adipocyte proliferation and hypertrophy are the root causes of adipose tissue expansion. Solute carrier family 25 member 28 (SLC25A28) is an iron transporter in the inner mitochondrial membrane.

Hypoxia-Related lncRNA Prognostic Model of Ovarian Cancer Based on Big Data Analysis.

Background: Hypoxia is regarded as a key factor in promoting the occurrence and development of ovarian cancer. In ovarian cancer, hypoxia promotes cell proliferation, epithelial to mesenchymal transformation, invasion, and metastasis. Long non-coding RNAs (lncRNAs) are extensively involved in the regulation of many cellular mechanisms, i.

A novel single-tube multiplex real-time PCR assay for genotyping of thiopurine intolerance-causing variant c.415C>T.

Thiopurines are commonly used in the treatment of acute lymphoblastic leukaemia and autoimmune conditions, can be limited by myelosuppression. The c.415C>T variant is strongly associated with thiopurine-induced myelosuppression, especially in Asians.

Combined treatment with a gastric inhibitory polypeptide receptor antagonist and a peptidyl peptidase-4 inhibitor improves metabolic abnormalities in diabetic mice.

Objectives: Dipeptidyl peptidase-4 inhibition and gastric inhibitory polypeptide (GIP) receptor antagonism have therapeutic effects in type 2 diabetes mellitus. We assessed the effects of sitagliptin and Pro(GIP) in a mouse model of diabetes.

Methods: Diabetes was induced in C57BL/6J mice by a high-fat diet and intraperitoneal injection of streptozocin.

[Restoration of orbicularis oculi muscle function with totally implanted artificial facial nerve].

To investigate the effect of the artificial facial nerve on the restoration of orbicularis oculi muscle function for unilateral peripheral facial paralysis in rabbit. Artificial facial nerve was implanted into the rabbit with unilateral peripheral facial paralysis between January 2018 and May 2019. At different time points after operation, the affected orbicularis oculi muscles' stimulation closure threshold and the synchronism about the motion of the two sides of orbicularis oculi muscles were monitored.

Case report on a misleading case of appendiceal perforation presented with severe generalized convulsion.

Appendicitis and convulsions are two common pathologies among children. Though appendicitis has some certain symptoms, they might present with atypical symptoms in young ages. Here we present a misleading case of a perforated appendix that presented with severe generalized convulsion, no significant abdominal symptoms and had a recent history of mild gastrointestinal problems.

Quetiapine attenuates cognitive impairment and decreases seizure susceptibility possibly through promoting myelin development in a rat model of malformations of cortical development.

Developmental delay, cognitive impairment, and refractory epilepsy are the most frequent consequences found in patients suffering from malformations of cortical development (MCD). However, therapeutic options for these psychiatric and neurological comorbidities are currently limited. The development of white matter undergoes dramatic changes during postnatal brain maturation, thus myelination deficits resulting from MCD contribute to its comorbid diseases.

Positive correlation between neovascularization degree of carotid atherosclerosis determined by contrast-enhanced ultrasound and level of serum C-reactive protein.

Background: Neovascularization is one of the most important risk factors for unstable plaque. This study was designed to correlate plaque thickness, artery stenosis and levels of serum C-reactive protein with the degree of intraplaque enhancement determined by contrast-enhanced ultrasound.

Patients And Methods: Contrast-enhanced ultrasound was performed on 72 carotid atherosclerotic plaques in 48 patients.

Association between portal vein thrombosis and survival of liver transplant recipients: a systematic review and meta-analysis of observational studies.

Background And Aims: Portal vein thrombosis (PVT) increases the technical complexity of liver transplantation (LT). This systematic review and meta-analysis aim to analyze the association of pre-LT PVT with the overall survival after LT.

Methods: PubMed, EMBASE, and Cochrane library databases were used to search for papers related to the association between pre-LT PVT and survival of LT recipients.

Low-dose X-ray irradiation promotes osteoblast proliferation, differentiation and fracture healing.

Great controversy exists regarding the biologic responses of osteoblasts to X-ray irradiation, and the mechanisms are poorly understood. In this study, the biological effects of low-dose radiation on stimulating osteoblast proliferation, differentiation and fracture healing were identified using in vitro cell culture and in vivo animal studies. First, low-dose (0.

Clinical analysis of 102 cases of Epstein-Barr virus infections in Chinese children.

The purpose of this study is to investigate the clinical manifestations and disease severity, to evaluate the recent trend of clinical manifestations and differences in the clinical and laboratory findings of EBV-associated IM (infectious mononucleosis) according to the age of children. We retrospectively collected cases on hospitalized patients a majority of 7 years old with characteristic symptoms of IM and serologically diagnosed EBV-associated IM at Shaanxi Provincial Peoples University Hospital in Xi'an from Apr, 2012 to Oct, 2013. All their medical records were reviewed and analyzed.