[Distribution characteristics and proteomic analysis of glioma-associated oncogene homolog1 positive cells during mouse orthodontic tooth movement].

To explore the distribution characteristics of glioma-associated oncogene homolog 1 (Gli1) positive cells during orthodontic tooth movement process and conduct a proteomic analysis of these cells. Forty Gli1-LacZ transgenic mice were used to establish an in orthodontic tooth movement (OTM) model for labeling Gli1 positive cells in Gli1-LacZ transgenic mice (OTM group) and an unforced control group, with tooth movement distance measured using micro-CT. The spatial relationship and distribution characteristics of Gli1 positive cells and H-type vessels of CD31 and endomucin (EMCN) in periodontal tissues were detected by immunofluorescence staining.

Transient Receptor Potential Ankyrin 1 (TRPA1) Mediated LPS-Induced Inflammation in Periodontal Ligament Stem Cells by Inhibiting the Phosphorylation of JNK.

Transient receptor potential ankyrin 1 (TRPA1) molecule is an important type of transient receptor potential (TRP) cation channels, which can cause extracellular Ca to flow into cells after activation. TRPA1 plays an important role in acute and chronic pain, inflammation, kidney disease, cough and asthma, osteoarthritis, cardiovascular disease, obesity, diabetes, and other diseases. In this study, the expression of interleukin (IL)-1, IL-6, and IL-8 in periodontal ligament stem cells (PDLSCs) treated by lipopolysaccharide (LPS) and the effect of LPS on PDLSCS proliferation were detected.

Mitochondrial transplantation reconstructs the oxidative microenvironment within fibroblasts to reverse photoaging.

Fibroblast-mediated oxidative stress is a pivotal factor in the pathogenesis of skin photoaging, predominantly induced by UVA radiation. Diverging from traditional strategies that concentrate on the reduction of reactive oxygen species (ROS), the present study implements mitochondrial transplantation as an innovative therapeutic approach. The objective of this study is to reestablish the oxidative microenvironment and to effectively rejuvenate cellular functionality through the direct introduction of healthy and vibrant mitochondria.

Machine Learning and Mendelian Randomization Reveal Molecular Mechanisms and Causal Relationships of Immune-Related Biomarkers in Periodontitis.

This study aimed to investigate the molecular mechanisms of periodontitis and identify key immune-related biomarkers using machine learning and Mendelian randomization (MR). Differentially expressed gene (DEG) analysis was performed on periodontitis datasets GSE16134 and GSE10334 from the Gene Expression Omnibus (GEO) database, followed by weighted gene co-expression network analysis (WGCNA) to identify relevant gene modules. Various machine learning algorithms were utilized to construct predictive models, highlighting core genes, while MR assessed the causal relationships between these genes and periodontitis.

The Application of the NOSES System to Treat Cheek Squamous Cell Carcinoma.

Purpose: Oral squamous cell carcinoma is one of the most common types of cancer in the head and neck. Squamous cell carcinoma of the buccal mucosa is an important part of it. The traditional surgical method leads to great injury and is accompanied by unacceptable scars.

Multidisciplinary treatment approach for complex crown-root fractures in child with periodontal health as the guiding principle: a case report with 8-year follow-up.

Background: Pediatric anterior teeth complex crown-root fractures has high incidence and pose greater treatment challenges. Dynamic tooth development increases risks for orthodontic, periodontal, and restorative treatments.

Case Description: The authors present a case of a 12-year-old boy with a complex crown-root fracture in the right maxillary central incisor.

Exploring the molecular mechanism of Taohong Siwu decoction in the treatment of non-small-cell lung cancer based on network pharmacology and molecular docking.

Objective: This study aimed to explore the mechanism of Taohong Siwu decoction (THSWD) in the treatment of non-small-cell lung cancer (NSCLC) by using comprehensive analysis.

Methods: The active components and relevant targets of THSWD were analyzed by network analysis to construct the active component-target-disease network diagram. Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were conducted on the core targets by the Metascape database.

Chinese expert consensus on the diagnosis and clinical management of medication-related osteonecrosis of the jaw.

Medication-related osteonecrosis of the jaw (MRONJ) is a side effect that occurs after treatment for systemic diseases. However, most institutions currently rely on empirical methods to make diagnosis and treatment plans, and there is a lack of consensus or guidelines for the classification, staging and treatment of MRONJ in China. To address this gap and improve prognosis, an expert panel representing 11 renowned domestic medical colleges and affiliated hospitals in China was convened.

Reconstructing through-and-through cheek defects using transposing cervicofacial and anterolateral thigh flaps-comparative analysis of aesthetic and functional outcomes.

Despite extensive efforts to restore through-and-through cheek defects caused by oral cavity malignancies, surgeons continue to explore optimal approaches to achieving satisfactory cosmetic and functional outcomes. In this prospective randomized controlled study, we share our experience with a modified flap technique, involving the transposition of cervicofacial and anterolateral thigh flaps to repair through-and-through cheek defects. A total of 41 patients with oral squamous cell carcinoma involving the cheek underwent radical excision of buccal carcinoma and reconstruction with a chimeric anterolateral thigh bi-paddles flap.

Nanozymes: a promising solution for dental antibacterial applications.

Dental diseases pose significant public health challenges globally, affecting millions with conditions exacerbated by microbial-induced inflammation. Traditional natural enzymes, despite their antibacterial and anti-inflammatory capabilities, are limited by operational stability and environmental sensitivity. This review explores the revolutionary realm of nanozyme-artificial enzymes made from nanomaterials-which offer enhanced stability, cost-effectiveness, and ease of modification.

Advantages and feasibility of prefabricated PEEK crowns for aesthetic restoration in primary teeth.

Objectives The main purpose of this study is to evaluate the efficacy of polyetheretherketone (PEEK) as a material for prefabricated crown restorations in pediatric dentistry, particularly for restoring primary tooth structure defects. Materials and methods This study analyzed the effects of three surface treatment modalities on PEEK's surface morphology, wettability (as measured by contact angle), and shear bond strength. These treatments included alumina (AlO) sandblasting alone, and in conjunction with a 98% sulfuric acid pretreatment.

Evaluating sagittal condylar inclination: a comparative analysis of various digital workflow measures.

Introduction: This study aims to compare sagittal condylar inclination (SCI) measurements derived from three workflows: intraoral scan (IOS) aligned with cone-beam computed tomography (CBCT), IOS aligned with facial scan (FS), and a jaw motion analyzer (JMA) system, in a cohort of young individuals with established normal occlusion. Additionally, the study aims to identify sources contributing to variance in these measurement approaches.

Methods: Twenty-four healthy individuals exhibiting normal occlusion were enrolled in this clinical trial.

Procoagulant, antibacterial and antioxidant high-strength porous hydrogel adhesives in situ formed via self-gelling hemostatic microsheets for emergency hemostasis and wound repair.

Procoagulant, antibacterial and analgesic hemostatic hydrogel dressing with high wet tissue adhesion, ultra-high burst pressure, and easy preparation shows huge promising for rapid hemostasis in emergencies, yet it remains a challenge. Herein, we propose hemostatic microsheets based on quaternized chitosan-g-gallic acid (QCS-GA) and oxidized hyaluronic acid (OHA), which merge the benefits of sponges, hydrogels, and powders for rapid hemostasis and efficient wound healing. Specifically, they exhibit a large specific surface area and excellent hydrophilicity, rapidly absorbing blood and self-gelling through electrostatic interaction and Schiff base crosslinking.

Impaired stemness in aging periodontal ligament stem cells is mediated by the progerin/endoplasmic reticulum stress/p53 axis.

Introduction: Decreased periodontal ligament stem cells (PDLSCs) stemness is a key factor in age-related alveolar bone loss. Endoplasmic reticulum (ER) stress is closely related to age-related diseases and the mesenchymal stem cell (MSC) stemness. However, the role of ER stress in regulating the stemness of senescent PDLSCs and its potential mechanism remain unclear.

Biomechanical effects of functional clear aligners on the stomatognathic system in teens with class II malocclusion: a new model through finite element analysis.

Objectives: The Functional Clear Aligner (FCA) is a novel orthodontic appliance designed for the treatment of Class II malocclusion with mandibular retrognathia in adolescents. The aim of this study was to investigate the biomechanical characteristics of the masticatory muscles, jawbone, and temporomandibular joint (TMJ) during mandibular advancement using either FCA or Class II elastics combined with clear aligner (Class II elastics) through finite element analysis.

Materials And Methods: A 3D finite element model of the 'muscle-jawbone-TMJ-appliance' system was constructed based on CBCT and MRI images of a boy with skeletal Class II malocclusion.

A Prediction Model for External Root Resorption of the Second Molars Associated With Third Molars.

Objectives: The aim of this study is to investigate risk factors for external root resorption (ERR) of second molars (M2) associated with impacted third molars (M3), and to develop a prediction model that can offer dentists a reliable and efficient tool for predicting the likelihood of ERR.

Methods: A total of 798 patients with 2156 impacted third molars were collected from three centres between 1 December 2018 and 15 December 2018. ERR was identified by cone beam computed tomography examinations.

BANF1 is a novel prognostic biomarker linked to immune infiltration in head and neck squamous cell carcinoma.

Background: Barrier-to-autointegration factor 1 (BANF1) is an abundant and ubiquitously expressed postnatal mammalian protein that is overexpressed in numerous human cancers and can promote cancer cell proliferation. However, the role of BANF1 in prognosis remains unclear in head and neck squamous cell carcinoma (HNSCC).

Methods: BANF1 expression data were obtained from the GEO and TCGA databases.

Evaluation of the effect of orthodontic treatment on the reliability of facial recognition by using three-dimensional model superimposition technique.

3D-3D registration of facial models, has great advantages in personal individual identification in forensic medicine. However, orthodontic treatment has brought changes in facial soft and hard tissues, which has a potential effect in personal identification. The aim of the study was to explore whether orthodontic treatment affects 3D-3D facial recognition.

[Three-dimensional analysis of force distribution on maxillary dentition while distalization of first and second molars simultaneously with clear aligners].

To explore the force distribution on the maxillary dentition when the first and second molars distalized simultaneously with different step sizes using clear aligners in order to provide a theoretical basis for the rational design of molar distalization. Clear aligners were designed to simultaneously distalize the maxillary first and second molars bilaterally, with rectangular attachments placed on the buccal surfaces of the first and second premolars, as well as the second molars. Based on different step sizes, the aligners were divided into three groups: Group A (0.

[Interpretation of a clinical practice guideline on the management of chronic pain associated with temporomandibular joint disorders].

Temporomandibular disorders (TMD) are a heterogeneous group of diseases that affect the temporomandibular joint, chewing muscle system, dental occlusion, and even various structures throughout the body, with significant characteristics of biological-psychological-social pattern. TMD related chronic pain, as the most important clinical symptom, can result in negative emotions seriously affecting patients' quality of life and physical and mental health. Although a variety of therapies have been previously reported to treat TMD related chronic pain, there is a lack of widely recognized therapies.

Hypoxia-induced NLRP3 inflammasome activation via the HIF-1α/NF-κB signaling pathway in human dental pulp fibroblasts.

Background: Previous studies have reported the link between hypoxic conditions and NLRP3 inflammasome-mediated pulpal inflammation in the progression of pulpitis. However, the underlying mechanism has not been fully elucidated. This study aimed to investigate the role of HIF-1α in the regulation of NLRP3 inflammasome pathway via NF-κB signaling under hypoxic conditions with or without LPS in human dental pulp fibroblasts (HDPFs) during the progression of pulpitis.

[Effects of clear aligners combined with maxillary protractor on mixed dentition patient with skeletal class Ⅲ malocclusion: a finite element study].

To compare the effects of expansion screw with maxillary protractor and clear aligners combined with maxillary protractor on the maxilla and maxillary dentition of mixed dentition patients with class Ⅲ skeletal malocclusion using finite element analysis, further providing clinical guidance for clear aligner treatment. A finite element model was established based on maxillofacial cone-beam CT data of a 10-year-old boy with mixed dentition, class Ⅲ skeletal malocclusion, who visited Department of Orthodontics, School of Stomatology, The Fourth Military Medical University in January, 2024. The expander with protractor group (group A) and the clear aligners with protractor group (group B) were divided.

[Early treatment of Class Ⅲ malocclusion based on craniomaxillofacial growth and development].

More and more attention has been paid to the early treatment of malocclusion, especially the early treatment of class Ⅲ malocclusion. Class Ⅲ malocclusion not only affects maxillofacial growth and development, occlusal function and facial beauty, but also leads to serious physical and mental problems. Focusing on class Ⅲ malocclusion, the definition, classification, etiological mechanism, craniofacial growth and development rule, and the influence of early treatment on craniofacial growth and development were reviewed.