Adaptive responses of Alicyclobacillus acidoterrestris in acidic broth and fruit juices: Focus on the influences of organic acids and temperature conditions.

Acid adaptive response (AAR) is a survival mechanism that allows bacteria to develop enhanced stress tolerance. Our previous research identified AAR in Alicyclobacillus acidoterrestris, a thermo-acidophilic bacterium responsible for fruit juice spoilage. However, the roles of specific acidulants, adaptive temperatures, and acidic juice matrices in triggering AAR remain elusive.

Single-cell RNA sequencing reveals intrahepatic signature related to pathobiology of duck hepatitis A virus type 3 (DHAV-3) infection.

DHAV-3 is one of the main causative agents of duck viral hepatitis (DVH), an acute and highly lethal infectious disease in duck industry. However, the understanding of the pathogenesis of this virus in ducklings is limited. To dissect the molecular characteristics associated with pathobiology of ducklings to DHAV-3, we applied single-cell RNA-sequencing approach to profile the transcriptome of 1.

Alterations of lung and gut microbiota in sodium butyrate alleviating heat stress-induced lung injury of broilers.

Heat-induced stress has a significant impact on the health of broilers. It induces panting behavior and elevates oxygen consumption, leading to considerable strain on the broiler lungs. However, the precise effects of heat stress on lung injury, along with changes in the lung and gut microbiota, are not yet fully understood.

Neuroprotective Indole Alkaloids from the Soil-Derived Fungus sp. XZ8.

A chemical investigation of the soil-derived fungus sp. XZ8 led to the isolation of five new indole alkaloids, trichindoles A-E (-), with diverse architectures, along with seven known analogues (-). Their structures were elucidated by extensive spectroscopic data analysis, and their absolute configurations were determined by single-crystal X-ray diffraction and modified Mosher's method.

Abscisic Acid, Microtubules and Phospholipase D-Solving a Cellular Bermuda Triangle.

Rice plants are important food crops that are sensitive to cold stress. Microtubules (MTs) are highly associated with plant response to cold stress. The exogenous application of abscisic acid (ABA) can transiently induce the cold stability of microtubules.

Traditional Chinese Medicine Monomer Bakuchiol Attenuates the Pathogenicity of via Targeting PqsR.

As the antibiotic resistance of pathogens becomes increasingly severe, it is becoming more feasible to use methods that suppress the virulence of pathogens rather than exerting selective pressure on their growth. , a dangerous opportunistic pathogen, infects hosts by producing multiple virulence factors, which are regulated by quorum-sensing (QS) systems, including the systems, systems, and systems. This study used the chromosome transcription fusion reporter model to screen the traditional Chinese medicine monomer library and found that bakuchiol can effectively inhibit the system and related virulence phenotypes of , including the production of virulence factors (pyocyanin, hydrogen cyanide, elastase, and lectin) and motility (swarming, swimming, and twitching motility) without affecting its growth.

Comparative Analysis of miRNA Expression Profiles of Yak Milk-Derived Exosomes at Different Altitudes.

Yaks are a rare and unique animal species inhabiting the Qinghai-Tibet Plateau; they are renowned for their remarkable ability to thrive in harsh environments. Milk-derived exosomes, tiny vesicles containing various biological molecules, play crucial roles in numerous pathological and physiological processes, including cell growth, development, and immune regulation. This study delved into the microRNA expression profiles of yak milk-derived exosomes collected from both high- and low-altitude populations using small RNA sequencing.

Decomposing disparities in the utilization of basic public health services between locals and internal migrants in China: the role of social determinants.

Background: Internal migrants in China have long been at a disadvantage in terms of access to publicly financed services, as well as the utilization of public health services. The aim of the study was to examine inequities in the use of basic public health services between internal migrants and the local population and estimate the factors that contributed to inequity in use.

Methods: The data for this study was derived from the 2017 wave of the China Migrants Dynamic Survey.

Novel Co-MOF-doped gelatin/agar intelligent film for beef freshness visual tracking based on the structural change of ZIF-67 under ammonia etching effect.

It is an important task to construct intelligent packaging for meat freshness monitoring with good color stability and indication function. Herein, cobalt-based metal-organic framework nanomaterials (Co-MOF, ZIF-67) with antimicrobial and ammonia-sensitive properties were successfully synthesized and added into gelatin/agar (GA) matrix to develop highly stable intelligent films (GA/ZIF67). The incorporation of ZIF-67 nanoparticles enhanced the hydrophobicity (water contact angle >90°) and UV-blocking properties (close to 0.

Pharmacological function of melanocortin-3 receptor in goldfish (Carassius auratus).

The melanocortin-3 receptor (MC3R) was recognized for its critical role in energy metabolism and inflammatory responses in mammals; however, its functions in fish remain poorly understood. This study characterized the mc3r gene in goldfish, investigating its sequence, tissue distribution, and pharmacological responses. The coding sequence of goldfish mc3r was 975 bp, translating to a 325-amino-acid protein typical of G protein-coupled receptors, with notable conservation across cyprinids.

Linkage and association analysis to identify wheat pre-harvest sprouting resistance genetic regions and develop KASP markers.

Unlabelled: Pre-harvest sprouting (PHS) of wheat ( L.) is one of the complex traits that result in rainfall-dependent reductions in grain production and quality worldwide. Breeding new varieties and germplasm with PHS resistance is of great importance to reduce this problem.

Engineering high-activity crosslinked enzyme aggregates via SpyCatcher/SpyTag-mediated self-assembly.

Crosslinked Enzyme Aggregates (CLEAs) are favored for their operational stability and recyclability. However, the traditional CLEAs preparation may distort the enzyme's active site and reduce activity. Therefore, we developed a universally applicable crosslinked SpyCatcher scaffold system designed for the facile preparation of CLEAs.

Multifunctional layer-by-layer smart film with betalains and selenium nanoparticles for intelligent meat freshness monitoring and preservation.

Multifunctional pH-responsive films were fabricated via layer-by-layer deposition of gelatin, chitosan, and carboxymethyl cellulose (CMC), incorporating selenium nanoparticles (SeNPs) and beetroot extract (BTE), to monitor and preserve beef freshness. SeNPs were synthesized and characterized via various techniques. BTE exhibited promising functional properties, and films demonstrated a significant color transition from red to yellow across pH 2-14.

Visible light-driven multichannel concerted antibacterial films with Photodynamically enhanced self-cascading Chemodynamic actions for long-term preservation of perishable food.

This work pioneered an innovative visible light-powered, self-cascading peroxide antimicrobial packaging system (RPFe-CS), featuring a photodynamic enhancement effect achieved through the demand-oriented design of riboflavin sodium phosphate and Fe coordination complexes (RPFe) fillers with photodynamic and peroxidase activities, and the ingenious selection of slightly acidic chitosan (CS) film matrix. In this system, the photo-responsive properties of RPFe particles not only generate the •O, •OH, and O required for photodynamic sterilization, but also the produced HO serves as a necessary substrate for peroxidase to exert its bactericidal effect, endowing the packaging system with a "self-production and self-marketing" cascade process. The RPFe-CS film achieved efficient eradication to bacteria and fungi reaching up to 99.

Structural Optimization of Natural Plant Products: Construction, Pesticidal Activities, and Toxicology Study of New 2-Isopropanol-4-methoxy-7-alkyl/aryloxycarbonyl-()-vinyl-2,3-dihydrobenzofurans.

Recently, the structural optimization of natural bioactive products has been one of the important ways to discover new pesticide candidates. Based on osthole as a lead compound, herein, a series of new 2-isopropanol-4-methoxy-7-alkyl/aryloxycarbonyl-()-vinyl-2,3-dihydrobenzofuran derivatives were synthesized. Steric configurations of compounds , , , , , , and were confirmed by X-ray monocrystallography.

The interaction of nutrient uptake with biotic and abiotic stresses in plants.

Plants depend heavily on efficient nutrient uptake and utilization for optimal growth and development. However, plants are constantly subjected to a diverse array of biotic stresses, such as pathogen infections, insect pests, and herbivory, as well as abiotic stress like drought, salinity, extreme temperatures, and nutrient imbalances. These stresses significantly impact the plant's ability to take up nutrient and use it efficiency.

Population sequencing of cherry accessions unravels the evolution of Cerasus species and the selection of genetic characteristics in edible cherries.

Cerasus is a subgenus of Prunus in the family Rosaceae that is popular owing to its ornamental, edible, and medicinal properties. Understanding the evolution of the Cerasus subgenus and identifying selective trait loci in edible cherries are crucial for the improvement of cherry cultivars to meet producer and consumer demands. In this study, we performed a de novo assembly of a chromosome-scale genome for the sweet cherry (Prunus avium L.

Unlocking dual-mode enzyme activities on bacterial surface: Directional recognition and swift capture of Alicyclobacillus acidoterrestris from fruit juices.

The acidophilic and heat-resistant characteristics of Alicyclobacillus acidoterrestris (A. acidoterrestris) pose significant challenges to fruit juice production. Traditional thermal removal methods are often ineffective against this resilient bacterium.

Photodynamic inactivation mediated by natural alizarin on bacteria for the safety of fresh-cut apples.

Most photosensitizers have limited responsiveness to visible light, however, visible light is a light source with a wide range of wavelengths and the most common in daily life, and making full use of visible light can help to enhance the photodynamic antimicrobial properties of photosensitizers. To tackle this issue, this study confirmed that alizarin has a good absorption capacity for visible light by UV-DRS analysis. Theoretical calculations showed that alizarin might be excited through the charge transfer (CT) mechanism.

METTL3-mediated mA modification regulates muscle development by promoting TM4SF1 mRNA degradation in P-body via YTHDF2.

N6-methyladenosine (mA), a well-known post-transcriptional modification, is implicated in diverse cellular and physiological processes. However, much remains unknown regarding the precise role and mechanism of mA modification on muscle development. In this study, we make observation that the levels of mA and METTL3 are markedly elevated during the differentiation phase (DM) compared to the growth phase (GM) in both C2C12 and bovine myoblasts.

Novel nanomaterials-based combating strategies against drug-resistant bacteria.

Numerous types of contemporary antibiotic treatment regimens have become ineffective with the increasing incidence of drug tolerance. As a result, it is pertinent to seek novel and innovative solutions such as antibacterial nanomaterials (NMs) for the prohibition and treatment of hazardous microbial infections. Unlike traditional antibiotics (e.

Oral Yeast-Cell Microcapsule-Mediated DNA Vaccines Against Induce Effective Intestinal Immunity and Modulate Gut Microbiota.

is a common opportunistic pathogen that causes gastrointestinal diseases in livestock and poultry. Our preliminary research has demonstrated that administering oral yeast-cell microcapsule (YCM)-mediated DNA vaccines can effectively stimulate mucosal immunity, thereby preventing the occurrence of gastrointestinal diseases. In this study, the α-toxin gene was first cloned and the H126G and C-terminal (C247-370) mutations were created.

Building of CuO@Cu-TA@DSF/DHA Nanoparticle Targets MAPK Pathway to Achieve Synergetic Chemotherapy and Chemodynamic for Pancreatic Cancer Cells.

With the increase of reactive oxygen species (ROS) production, cancer cells can avoid cell death and damage by up-regulating antioxidant programs. Therefore, it will be more effective to induce cell death by using targeted strategies to further improve ROS levels and drugs that inhibit antioxidant programs. Considering that dihydroartemisinin (DHA) can cause oxidative damage to protein, DNA, or lipids by producing excessive ROS, while, disulfiram (DSF) can inhibit glutathione (GSH) levels and achieve the therapeutic effect by inhibiting antioxidant system and amplifying oxidative stress, they were co-loaded onto the copper peroxide nanoparticles (CuO) coated with copper tannic acid (Cu-TA), to build a drug delivery system of CuO@Cu-TA@DSF/DHA nanoparticles (CCTDD NPs).