PCR-based characterization of nasal and ocular surface flora and functional analysis of bacterial metabolic pathways in congenital nasolacrimal duct obstruction.

Purpose: To evaluate the role of Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, Serratia marcescens, and Moraxella catarrhalis in the nasal and ocular surface flora, along with their metabolic activities in children with unilateral congenital nasolacrimal duct obstruction (CNLDO).

Methods: Swabs were taken from the bilateral inferior meatus and ocular surface of 26 children with unilateral CNLDO before probing. Nasal and ocular surface swabs from non-operated eyes of children who underwent unilateral blepharoptosis or strabismus surgery formed the control group.

Constructing High-Yielding for (-)-α-Bisabolol Production Based on the Exogenous Haloarchaeal MVA Pathway and Endogenous Molecular Chaperones.

(-)-α-Bisabolol exhibits analgesic, anti-inflammatory, and skin-soothing properties and is widely applied in the cosmetic and pharmaceutical industries. The use of plant essential oil distillation or chemical synthesis to produce (-)-α-bisabolol is both inefficient and unsustainable. Currently, the microbial production of (-)-α-bisabolol mainly relies on and as chassis strains; however, high concentrations of (-)-α-bisabolol have certain toxicity to the strain.

Herbicide-treated soil as a reservoir of beneficial bacteria: microbiome analysis and PGP bioinoculants in maize.

Background: Herbicides are integral to agricultural weed management but can adversely affect non-target organisms, soil health, and microbiome. We investigated the effects of herbicides on the total soil bacterial community composition using 16S rRNA gene amplicon community profiling. Further, we aimed to identify herbicide-tolerant bacteria with plant growth-promoting (PGP) capabilities as a mitigative strategy for these negative effects, thereby promoting sustainable agricultural practices.

Epidemiological and genetic characteristics of clinical carbapenemase-producing Enterobacterales isolates from Batna hospitals in Algeria.

Background: Carbapenemase-producing Enterobacterales isolates are associated with significant mortality and have emerged as a major problem in healthcare settings worldwide.

Objective: Our aim was to investigate the epidemiological and genotypic characteristics of carbapenemase-positive Enterobacterales isolates from patients hospitalised in three hospitals in the city of Batna, Algeria.

Methods: Between 2016 and 2019, a total of 5,316 clinical isolates were obtained.

Impact of gestational diabetes mellitus in gut and human breast milk microbiome in Colombian women and their infants.

Human breast milk (HBM) is a vital source of macronutrients and micronutrients that are crucial for an infant's development. Recent studies have shown that HBM contains diverse microorganisms, including bacteria, viruses, protozoa, and anaerobic fungi. Additionally, novel research has revealed that individuals with metabolic disorders, such as diabetes mellitus, are prone to dysbiosis in their gut microbiome.

Genome-wide identification of bacterial genes contributing to nucleus-forming jumbo phage infection.

The Chimalliviridae family of bacteriophages (phages) form a proteinaceous nucleus-like structure during infection of their bacterial hosts. This phage 'nucleus' compartmentalises phage DNA replication and transcription, and shields the phage genome from DNA-targeting defence systems such as CRISPR-Cas and restriction-modification. Their insensitivity to DNA-targeting defences makes nucleus-forming jumbo phages attractive for phage therapy.

Coimmobilized Dual Enzymes in a Continuous Flow Reactor for the Efficient Synthesis of Optically Pure γ/δ-Lactones.

Enzyme catalysis is a promising method for producing chiral chemicals with high stereoselectivity under mild conditions. However, the traditional batch reaction suffers from low enzyme stability, low cofactor recycling, and poor enzyme reusability. Here, we present a continuous-flow method using coimmobilized dual enzymes for the synthesis of chiral γ-/δ-lactones, which are widely used in fragrances and flavors.

Assessment of microbial antagonistic activity and Quorum Sensing Signal Molecule (Cyclopeptides-DKPs and N-Acyl Homoserine Lactones) detection in bacterial strains obtained from avocado thrips (Thysanoptera: Thripidae).

The control of avocado pests and diseases heavily relies on the use of several types of pesticides, some of which are strictly monitored or not internationally accepted. New sources of bioactive molecules produced by phytopathogen-inhibiting microorganisms offer an excellent alternative for the control of pests and diseases. This study explores the potential antagonistic action against phytopathogenic microorganisms, using bacterial strains obtained from avocado thrips.

A Novel Approach to Identifying Appropriate Candidates for Transvenous Lead Extraction.

Background: The need for transvenous lead extractions (TLEs) in the setting of cardiac implantable electronic device-(CIED) related infections continues to rise. Delays in referral for TLE in this setting are common and are associated with increased mortality.

Objective: To describe the outcomes of a comprehensive approach, including an electronic medical record (EMR)-based notification algorithm designed to identify patients with active CIED-related infections to facilitate timely TLE.

Draft genome sequence data of strain harboring from Dhaka, Bangladesh.

Here, the draft genome sequence of a multi-drug resistant (MDR) strain BMD28, isolated from a clinical source from Dhaka, Bangladesh, has been reported. The sequence raw read files were generated using Illumina sequencing technology utilizing genomic DNA from the pure culture of this strain. The strain has a genome size of around 5.

Microbiome-Metabolomics Analysis Insight into the Effects of Starvation and Refeeding on Intestinal Integrity in the Juvenile Largemouth Bass ().

The effects of starvation and refeeding on the gut condition of juvenile largemouth bass () remain unclear. Therefore, our research aimed to explore these effects. Amylase and lipase activities were remarkably decreased in the starvation (ST) group, yet prominently increased in the refeeding (RE) group ( < 0.

Enhancing Antimicrobial Efficacy of Sandalwood Essential Oil Against for Food Preservation.

The growing emphasis on food safety and healthier lifestyles, driven by industrial expansion and scientific priorities, has highlighted the necessity of managing harmful microorganisms to guarantee food quality. A significant challenge in this domain is the control of pathogens that are capable of forming biofilms, entering a sessile state that enhances their resistance to broad-spectrum antibiotics. Essential oils, renowned for their antibacterial properties, present a promising natural alternative for food preservation.

Multi-conflict islands are a widespread trend within Serratia spp.

Bacteria carry numerous anti-phage systems in "defense islands" or hotspots. Recent studies have delineated the content and boundaries of these islands in various species, revealing instances of islands that encode additional factors, including antibiotic resistance genes, stress genes, type VI secretion system (T6SS)-dependent effectors, and virulence factors. Our study identifies three defense islands in the Serratia genus with a mixed cargo of anti-phage systems, virulence factors, and different types of anti-bacterial modules, revealing a widespread trend of co-accumulation that extends beyond T6SS-dependent effectors to colicins and contact-dependent inhibition systems.

Exploration and impact of Metlaoui-Gafsa phosphate rock amendment: the role of Serratia plymuthica BMA1 in phosphate solubilization, heavy metal rhizoaccumulation, and enhanced nutrition in Vicia faba L.

The geochemical analysis of Gafsa rock phosphate (GRP) revealed relatively high concentrations of essential plant minerals and trace heavy metals (HMs). Environmental contamination factors indicated moderate to very strong HM contamination due to GRP soil amendment. The potential use of the Serratia plymuthica BMA1 strain, which is known for its ability to solubilize GRP, to enhance mineral nutrition in Vicia faba L.

A complete DNA repair system assembled by two endosymbionts restores heat tolerance of the insect host.

DNA repair systems are essential to maintain genome integrity and stability. Some obligate endosymbionts that experience long-term symbiosis with the insect hosts, however, have lost their key components for DNA repair. It is largely unexplored how the bacterial endosymbionts cope with the increased demand for mismatch repairs under heat stresses.

Characterization of YdgH: a mediator of beta-lactam susceptibility in Enterobacterales.

Beta-lactam antibiotics are often the treatment of choice for serious bacterial infections. In a previous screen for novel genetic mediators affecting beta-lactam susceptibility, we discovered that deletion of , a conserved gene of unknown function, leads to increased resistance to beta-lactams, as well as increased susceptibility to detergent compounds. Here, we further characterize YdgH in , and using a combination of biochemical and cell biological approaches.

Changes in antibiotic resistance patterns of Gram-negative bacilli across three different wastewater treatment plants in northwest Algeria; first comparative study.

Changes in antibiotic resistance of bacteria in three different wastewater treatment plants (WTP) were investigated to determine their role they play in the dissemination of multidrug resistant bacteria (MDR). Water samples were collected from downstream of three (WTP) from where pollution parameters were detected and high-performance Liquid chromatography (HPLC) coupled with an ultraviolet (UV) detector was used to measure antibiotic residues. In addition, 88 bacilli Gram-negative (BGN) were collected; activated sludge, aerated lagoons and natural lagoons, and tested for resistance against 18 βeta-lactam antibiotics.

Polystyrene nanoplastics sequester the toxicity mitigating potential of probiotics by altering gut microbiota in grass carp (Ctenopharyngodon idella).

This study evaluated the role of probiotics in enhancing intestinal immunity and mitigating polystyrene nanoplastics (PS-NPs)-induced toxicity in grass carp (Ctenopharyngodon idella). Grass carp were fed probiotics (Bacillus subtilis, Bacillus velezensis, Lactobacillus reuteri, and Lactococcus lactis) for two weeks before being exposed to PS-NPs for five days. Probiotic pretreatment alleviated PS-NPs-induced intestinal damage, with Bacillus velezensis and Lactococcus lactis groups showing milder vacuolation and villus breakage than other groups.

Maternal Prenatal Stress and the Offspring Gut Microbiome: A Cross-Species Systematic Review.

The prenatal period is a critical developmental juncture with enduring effects on offspring health trajectories. An individual's gut microbiome is associated with health and developmental outcomes across the lifespan. Prenatal stress can disrupt an infant's microbiome, thereby increasing susceptibility to adverse outcomes.

Serratia marcescens Prosthetic Valve Endocarditis: Portending a Dismal Course.

 an opportunist pathogen mainly isolated in healthcare-associated infections, is a rare cause of infective endocarditis (IE) that generates an increased mortality rate compared to the usual agents. We report a case of a 70-year-old male patient who underwent a mitral valve replacement and was readmitted two months later with a high-grade continuous fever and deterioration of the general status. The diagnosis of early IE due to was established upon further investigation.

A Comprehensive Analysis of the Epidemiological and Genomic Characteristics of Global Serratia marcescens.

Background: Serratia marcescens outbreaks present significant challenges in clinical treatment, necessitating a deeper understanding of its epidemiological and genomic traits.

Objective: To analyze the epidemiological and genomic characteristics of S. marcescens at a global scale.

Genomes of novel strains from suburban soil.

Here, we present genomes of three strains of initially isolated from suburban soil-one strain of and two strains of -resistant to multiple classes of antibiotics. This expands the genomic sampling of a group relevant to the ecosystem and human health.