Ten chloroplast DNA primer pairs were used to amplify non-coding regions of cpDNA in eight mulberry genotypes and analyze genetic variations for classification purposes.
The study found that 10 primer pairs successfully produced results, yielding 152 marker loci and distinguishing the tested genotypes into two groups based on their cpDNA digestion patterns.
Analysis revealed that there were specific deletion fragments and base point mutations in the D-T region, which could enhance understanding of genetic diversity and be useful for phylogenetic analysis and pedigree identification.