Probing the effects of dextran-coated CeO nanoparticles on lung fibroblasts using multivariate single-cell Raman spectroscopy.

In this study, we investigated the cytotoxic effect of highly soluble dextran-coated CeO nanoparticles on human fetal lung fibroblasts MRC-5. We examined individual nanoparticle-treated cells by Raman spectroscopy and analyzed Raman spectra using non-negative principal component analysis and k-means clustering. In this way, we determined dose-dependent differences between treated cells, which were reflected through the intensity change of lipid, phospholipid and RNA-related Raman modes.

Structure and hormonal output of the adrenal gland after experimental estrogenization of male rats.

Orchidectomy and estrogenization of the male represent a procedure that is applicable in sex reassignment or in prostate cancer therapy. This approach has an influence on the hypothalamic-pituitary-adrenal axis and thus affects cardiovascular function and metabolism. We utilized orchidectomized rats to evaluate the effects of estradiol on the structure and hormonal output of the adrenal gland.

Academic outcomes of inclusive education in typically developing children.

Introduction: The fact that inclusive education has existed in Bosnia and Herzegovina for twenty years opens the question of how it affects typically developing children, among other things. This paper aims to examine the differences in general knowledge and mathematics of typically developing students with regard to whether they attend classes that include students with intellectual disabilities or not, as well as to determine the relationship of their knowledge with teachers' characteristics and the inclusiveness of schools they attend.

Methods: The sample included 331 students from 18 regular elementary school classes.

Mesenchymal Stem Cell-Sourced Exosomes as Potentially Novel Remedies for Severe Dry Eye Disease.

Severe dry eye disease (DED) is an inflammatory condition characterized by a lack of sufficient moisture or lubrication on the surface of the eye, significantly impacting the quality of life and visual function. Since detrimental immune response is crucially responsible for the development and aggravation of DED, therapeutic agents which modulate phenotype and function of eye-infiltrated inflammatory immune cells could be used for the treatment of severe DED. Due to their potent immunomodulatory properties, mesenchymal stem cells (MSCs) represent potentially new remedies for the treatment of inflammatory eye diseases.

The association of origin and environmental conditions with performance in professional IRONMAN triathletes.

We have (i) little knowledge about where the fastest professional IRONMAN triathletes originate from and where the fastest races take place and (ii) we have no knowledge of the optimal weather conditions for an IRONMAN triathlon. The aims of the present study were, therefore, (i) to investigate the origin and the fastest IRONMAN race courses for professional triathletes and (ii) to evaluate the best environmental conditions (i.e.

NO-mediated DNA damage induced by polystyrene nanoparticles triggers program cell death in mesenchymal stem cells.

Daily contact with considerable amounts of polystyrene nanoparticles (PSNPs) may cause harmful effects on the living organisms, through mechanisms that are not fully understood. The study aimed to evaluate the cytotoxic and genotoxic effects of PSNPs (size 200 nm and 40 nm) in mesenchymal stem cells (MSCs). In order to estimate cellular uptake and retention of nanoplastics, PSNP-treated cells have been analyzed by transmission electron microscopy.

Essential Oil Variability of Juniperus oxycedrus s.l. Taxa in Southeast Europe - a Chemophenetic Approach.

The essential oil composition of 14 natural populations of two Juniperus oxycedrus s.l. taxa (J.

Sample Preparation of Adherent Cell Lines for Flow Cytometry: Protocol Optimization-Our Experience with SW-480 colorectal cancer cell line.

The most common method for detection of apoptosis is flow cytometry. In previously published studies there are some uncertainties and problems about the preparation of adherent cell lines for analysis. Thus, the aim of this study is to determine and describe how preparing the sample of SW-480 cells in two different ways affects the reliability of the results.

Detection of respiratory frequency rhythm in human alpha phase shifts: topographic distributions in wake and drowsy states.

Introduction: The relationship between brain activity and respiration is recently attracting increasing attention, despite being studied for a long time. Respiratory modulation was evidenced in both single-cell activity and field potentials. Among EEG and intracranial measurements, the effect of respiration was prevailingly studied on amplitude/power in all frequency bands.

The influence of different crosslinking agents onto the physical properties, integration behavior and immune response of collagen-based barrier membranes.

This study investigates the mechanical properties as well as and cyto- and biocompatibility of collagen membranes cross-linked with glutaraldehyde (GA), proanthocyanidins (PC), hexamethylendiisocyanate (HMDI) and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EC/NHS). A non-crosslinked membrane was used as reference control (RF). The initial cytotoxic analyses revealed that the PC, EC, and HMDI crosslinked membranes were cytocompatible, while the GA crosslinked membrane was cytotoxic and thus selected as positive control in the further study.

A serological survey of wild boar in Serbia for and .

and are causative agents of the porcine respiratory disease complex. However, information on the prevalence of these pathogens in wild boars is scarce. This study aimed to investigate the presence of antibodies to and in wild boars in Serbia.

The impact of sports experience on manual dexterity performances in school-age children.

Background: Manual dexterity is the ability to manipulate objects with precision and efficiency, using hands and fingers to achieve a specific objective. This study investigated how the practice of Capoeira, a sport that stimulates coordination skills, affects manual dexterity in children regularly engaged in physical activity or in sedentary children.

Methods: Eighty-four participants were enrolled in this study, including forty-six males and thirty-eight females (age: 8.

Smoking patterns and outcomes of severe sars-CoV-2 infection: a retrospective cohort study.

The purpose of this study was to analyze the association between the number of cigarettes smoked and the length of smoking with mortality among patients who were hospitalized in the intensive care unit (ICU) due to SARS-CoV-2 infection. This retrospective cohort study was conducted at the General Hospital in Sombor (Serbia). Patients who were hospitalized because of severe SARS-CoV-2 infection between March 2021 and March 2023 were included in this study.

Trust in scientists and their role in society across 68 countries.

Science is crucial for evidence-based decision-making. Public trust in scientists can help decision makers act on the basis of the best available evidence, especially during crises. However, in recent years the epistemic authority of science has been challenged, causing concerns about low public trust in scientists.

Perceptions of science, science communication, and climate change attitudes in 68 countries - the TISP dataset.

Science is integral to society because it can inform individual, government, corporate, and civil society decision-making on issues such as public health, new technologies or climate change. Yet, public distrust and populist sentiment challenge the relationship between science and society. To help researchers analyse the science-society nexus across different geographical and cultural contexts, we undertook a cross-sectional population survey resulting in a dataset of 71,922 participants in 68 countries.

Bilateral facial nerve palsy in a patient with West Nile neuroinvasive disease.

Introduction: Bilateral facial nerve palsy (FNP) is a rare condition that is idiopathic in only 20%. FNP is the most common cranial neuropathy in West Nile neuroinvasive disease (WNND) but is usually unilateral and only a few cases of bilateral FNP have been reported.

Case: We present a case of a 65-year-old woman with confirmed WNND and simultaneous bilateral FNP.

A ten-year retrospective analysis of risk factors and comorbidities prevalence in a group of septic arthritis patients.

Objectives: The aim of this study was the evaluation of a group of patients treated at the Department of Orthopaedics and Traumatology of Locomotory Apparatus at Luis Pasteur University Hospital in Košice for septic arthritis in relation to risk factors and chronic diseases and its microbial aetiologic profile.

Methods: We conducted a retrospective study of patients including all episodes of septic arthritis from March 2013 to August 2022. The occurrence of chronic diseases, risk factors and its microbiological profile were investigated.

Managing novel therapies and concomitant medications in chronic lymphocytic leukemia: key challenges.

The treatment of chronic lymphocytic leukemia (CLL) consists of the continuous use of Bruton tyrosine kinase inhibitors (BTKis) such as ibrutinib, acalabrutinib, zanubrutinib and pirtobrutinib, or Bcl-2 inhibitors, such as venetoclax. Overall survival (OS) and progression-free survival (PFS) of CLL patients are significantly improved with the use of these therapies. Adverse effects (AEs) that can occur during treatment and the presence of pre-existing comorbidities in patients can influence subsequent treatment outcomes and, consequently, OS and PFS.

Friction in soft biological systems and surface self-organization: the role of viscoelasticity.

Friction is a critical factor in the proper functioning of human organs as well as in the potential development of disease. It is also important for the design of diagnostic and interventional medical devices. Nanoscale surface roughness, viscoelastic or plastic deformations, wear, and lubrication all influence the functions of individual cells.

Associations of night eating with body mass index and waist circumference among health sciences students living in a post-conflict region.

Background: Stressors associated with the ongoing ethnic tensions may be the drivers of more frequent night eating, and subsequently, a higher body mass index.

Aim: To examine the association of night eating with body mass index (BMI) and waist circumference among health sciences students in Northern Kosovo.

Methods: A cross-sectional study was carried out at the University of Prishtina in Kosovska Mitrovica from November 2018 to March 2019.

Incorporating ethics into infectious disease clinical practice guidelines.

Background: Incorporating ethics into clinical practice guidelines (CPGs) can be challenging. This is particularly evident for infectious diseases (ID) CPGs due to the complexity of ID ethics (IDE) and the multiplicity of populations at-risk for infections.

Objectives: The OPENING project (IncOrPorating Ethics iN ClINical Guidelines: Practical Indications) was initiated by the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Ethics Advisory Committee (EEAC) in collaboration with the ESCMID Guidelines Subcommittee to generate guidelines for the systematic inclusion of ethics principles into ID CPGs.

The first geospatial dataset of irrigated fields (2020-2024) in Vojvodina (Serbia).

Irrigation is a cornerstone of global food security, enabling sustainable agricultural production and helping to ensure that food is available for people around the world, now and in the future. Mapping irrigated fields provides valuable information for sustainable water management, agricultural development, and environmental conservation efforts. However, the collection of high-quality training data, which is necessary for accurate irrigation mapping remains costly and labour-intensive.