RNA-seq analysis revealed genes associated with neuropathic pain induced by chronic compressive injury in interferon regulatory factors 4 knockout mice.

Objective: To explore the differential expression of genes between wild-type chronic compressive injury (CCI) mice (WT-CCI) and interferon regulatory factors 4 (IRF4) knockout CCI mice (KO-CCI) by RNA-seq analysis of the mouse spinal cord.

Methods: RNA-seq analysis of the spinal cord tissue of the chronic sciatic nerve ligation mice and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were used.

Results: A total of 104 genes were up-regulated and 116 genes were down-regulated in spinal cord of the mice in IRF4 knockout (KO-CCI) group compared with that in the wild-type CCI (WT-CCI) group.

Community grid management is an important measure to contain the spread of novel coronavirus pneumonia (COVID-19).

The outbreak of novel coronavirus pneumonia (coronavirus disease 2019 (COVID-19)), declared as a 'global pandemic' by the World Health Organization (WHO), is a public health emergency of international concern (PHEIC). The outbreak in multiple locations shows a trend of accelerating spread around the world. China has taken a series of powerful measures to contain the spread of the novel coronavirus.

Roles of CaMKIIβ in the neurotoxicity induced by ropivacaine hydrochloride in dorsal root ganglion.

Neurotoxicity of local anesthetics is often reported in the clinic, more and more people pay attention to them. CaMKIIβ, a subtype of CaMKII, is detected in the central nervous system. Previous study found that CaMKIIβ mRNA are up-regulated in DRG neurons treated with ropivacaine hydrochloride, as well as inhibition of Cav3.

Appropriate treatment sessions of flexible bronchoscopic balloon dilation for patients with nonmalignant central airway stenosis.

Background: Balloon dilation is a primary treatment for nonmalignant tracheobronchial stenosis, but often requires multiple treatment sessions to maintain therapeutic efficacy. No guideline is available to suggest the appropriate maximum number of treatment sessions. This study aimed to investigate the relationship between the number of balloon dilation sessions and its long-term therapeutic effectiveness in Chinese patients with nonmalignant central airway stenosis.

[Radiolabelling of a lung cancer-targeting small molecule polypeptide with (131)I and its radioactivity distribution in normal rabbits].

Objective: To establish a labeling method for a specific lung cancer-targeting small molecule peptide cNGQGEQc with ¹³¹I and observe the radioactivity distribution of the labeled peptide in rabbits using single-photon emission computed tomography (SPECT).

Methods: Chloramine-T method was used for ¹³¹I labeling of the tyrosine amino group on cNGQGEQc, and the labeling efficiency and radiochemical purity of ¹³¹I-cNGQGEQc were determined with paper chromatography. The stability of ¹³¹I-cNGQGEQc in saline and human serum was assessed after incubation in water bath at 37 degrees celsius; for 24 h.

A study of the histologic and enzyme histochemical changes in the cochlea of Guinea pigs after non-focused ultrasound irradiation.

We evaluated the histologic and histochemical (succinate dehydrogenase, SDH) changes in cochleas of guinea pigs after non-focused ultrasound (NFU) irradiation. For this purpose, the cochleas of 50 guinea pigs (both ears in Groups 1-10) were irradiated at 2.5-8.

[Clinical treatment of open pelvic fractures associated with perineal injury].

Objective: To investigate the method and the effectiveness of open pelvic fractures associated with perineal injury.

Methods: Between August 2000 and July 2010, 16 cases of open pelvic fractures associated with perineal injury were treated. There were 12 males and 4 females with an average age of 41 years (range, 17-69 years).

[Clinical analysis of serum endothelin in type 2 diabetic patients with vascular complications].

Objective: To explore the clinical significance of changes in urine microalbumin (UM) and endothelin (ET) in different courses of type 2 diabetes.

Methods: A clinical analyses of 30 type 2 diabetic patients with vascular complications (group A) was conducted in comparison with type 2 diabetic patients without vascular complications (group B) and patients with impaired glucose tolerance (IGT, group C). With 30 healthy subjects with family history of type 2 diabetes (group D) and 30 healthy subjects without such family history (group E) as controls, UM and ET were determined in all the subjects for a statistical analysis.

[Relationship between serum vWF and PAF in type 2 diabetic patients and diabetic nephropathy].

Objective: To observe the changes in urine albumin (UALB), von Willebrand factor (vWF) and platelet-activating factor (PAF) in different courses of type 2 diabetes.

Methods: Levels of UALB, vWF and PAF were determined in 30 type 2 diabetic nephropathy patients(group A), type 2 diabetic patients without DNP(group B, n=30), patients with impaired glucose tolerance (group C, n=30), the first-degree relatives of type 2 diabetic patients with normal glucose tolerance(group D, n=30) and 30 normal glucose tolerance subjects without family history of type 2 diabetes(group E).

Results: UALB and PAF contents were significantly higher in group A than in the other groups (P<0.

[Association of body mass index with diabetes in the elderly in Foshan].

Objective: To study the correlation between body mass index (BMI) and diabetes in the elderly residents in Foshan.

Methods: A total of 3 382 people above 60 years old participated in this questionnaire-based survey, with their blood pressure, height, body weight, and blood glucose measured and oral glucose tolerance test performed.

Results: The prevalence rates of diabetes in the elderly subjects with obesity, overweight, normal weight, and underweight were 31.