Correlation between serum miR-154-5p and urinary albumin excretion rates in patients with type 2 diabetes mellitus: a cross-sectional cohort study.

This study aimed to investigate the correlation between serum miR-154-5p and urinary albumin to creatinine ratio (UACR) in patients with type 2 diabetes mellitus (T2DM) and the association with biomarkers of inflammation and fibrosis in diabetic kidney disease (DKD). A total of 390 patients with T2DM were divided into three groups: normal albuminuria (UACR < 30 mg/g, n = 136, NA), microalbuminuria (UACR at 30-300 mg/g, n = 132, MA), and clinical albuminuria (UACR > 300 mg/g, n = 122, CA). Circulating miR-154-5p, inflammatory (C-reactive protein (CRP); erythrocyte sedimentation rate (ESR); and tumor necrosis factor-α (TNF-α) and fibrotic markers (vascular endothelial growth factor (VEGF); transforming growth factor-β1 (TGF-β1); and fibronectin (FN)), and other biochemical indicators were assessed via real-time PCR, enzyme-linked immunosorbent assay, and chemiluminescence assay in patients with T2DM and 138 control subjects (NC).

[Reconstruction of segmental bone defect by gene modified tissue engineering bone combined with vascularized periosteum].

Objective: To evaluate the therapeutic effect of bone morphogenetic protein 2 (BMP-2) gene modified tissue engineering bone (GMB) combined with vascularized periosteum in the reconstruction of segmental bone defect.

Methods: Adenovirus carrying BMP-2 gene (Ad-BMP-2) was transfected into the isolated and cultured rabbit bone marrow stromal cells (MSCs). The transfected MSCs were seeded on bovine cancellous bone scaffolds (BCB) to construct gene modified tissue engineering bone (GMB).

[Comparison between gene therapy and gradual release carrier for bone morphogenetic protein-2 in repairing bone defects].

To compare the effects between gene therapy and gradual release carrier for bone morphogenetic protein-2 (BMP-2) in repairing bone defects, bone defects for 15 mm were created.on the bilateral radius in rabbits and treated with four kinds of implantations, ie, composite of transgeneic MSCs and PLA/PCL (Group A), composite of MSCs and gradual release carrier for BMP-2 (Group B), composite of MSCs and PLA/PCL (Group C), and PLA/PCL alone (Group D). After 4, 8, and 12 weeks of the operations, X-ray, histological examination, biomechanics analysis, and bone density measurement were conducted.

[BMP-2 gene carried by biodegradable scaffold and fibrinous gel for repairing segmental radial defect in rabbit].

Adenovirus carrying BMP-2 gene, after being mixed with fibrinous gel, was siphoned off on biodegradable scaffolds (PLA/PCL). The composite was used to repair 1.5 cm long radius defect in rabbits.

[The relationship between Oddi's sphincter and bile duct pigment gallstone].

Objective: To investigate the relationship between anatomic abnormalities and malfunction of Oddi sphincter with formation of bile duct pigment gallstone.

Methods: One hundred and twenty-three patients with a T tube after cholecystectomy and choledochotomy were divided into reflux group and control group by measuring the amounts of radioactivity of (99m)Tc-DTPA in the bile. Among them 53 were selected randomly to undergo choledochoscopic manometry.

[Effects of Genistein on the proliferation and expression of survivin in salivary adenoid cystic carcinoma cell line SACC-83].

Objective: To investigate the anti-proliferation effect of tyrosine protein kinase inhibitor, Genistein, on human salivary adenoid cystic carcinoma cell line SACC-83, and its effect on Survivin expression.

Methods: SACC-83 cells were treated with different concentration Genistein for different time, cell survival rate was calculated with MTT assay, apoptosis was detected with flow cytometry, the expression of Survivin was quantitatively analyzed by Western blotting and FluorChem V2.0 software.

[Behavioral patterns of autistic children during infancy].

Objective: The present study investigated the behavioral patterns of autistic children during infancy to provide clues for early identification of childhood autism.

Methods: The abnormal behaviors of 30 children with autism and 26 children with other developmental disorders in infancy were investigated.

Results: The children with autism presented a series of abnormal behaviors, including no social smile, no eye contact and no respond to own name, and joint attention deficiency, which were distinguished from the children with other developmental disorders.

[Vascularization in transplantation of gene modified tissue engineered bone for repairing bone defect].

Objective: To study the vascularization of the composite of bone morphogenetic protein 2 (BMP-2) gene transfected marrow mesenchymal stem cells (MSCs) and biodegradable scaffolds in repairing bone defect.

Methods: Adenovirus vector carrying BMP-2 (Ad-BMP-2) gene transfected MSCs and gene modified tissue engineered bone was constructed. The 1.

[Effects of BMP-2 gene therapy on vascularization in repairing bone defects].

Objective: To study the effects of BMP-2 gene therapy on vascularization in repairing bone defects.

Methods: The isolated rabbit mesenchymal stem cells (rBMSC), after being transfected by adenovirus carrying BMP-2 gene (Ad-BMP-2) and seeded on xenogeneic bone scaffolds, were used to repair 1.5 cm-long radius bone defects.

Comments and controversies: magnetic resonance spectroscopy and gliomas.

In vivo proton magnetic resonance spectroscopy (1HMRS) can substantially improve the non-invasive categorization of human brain tumors, especially for gliomas. It provides greater information concerning tumor activity and characterization of the tumor tissue than is possible with MRI techniques alone. Moreover, 1HMRS may ultimately prove to be a highly beneficial modality in the post-irradiation care of patients with brain gliomas.

[Expression and significance of PTEN protein in tongue squamous cell carcinoma].

Purpose: To study the expressions and clinical significance of PTEN protein in tongue squamous cell carcinoma (TSCC).

Methods: The expressions of PTEN at protein level was investigated in 74 cases of TSCC and 15 cases of normal peripheral tissues around cancer (as control) by strep avidin-biotin complex (SABC) immunohistochemical technique. Mann-Whitney U-test, Pearson's Chi-square test and Fisher exact test were used to analyze the data.

Endoscopic ultrasound-guided interstitial brachytherapy of unresectable pancreatic cancer: results of a pilot trial.

Background And Study Aims: Intraoperative interstitial brachytherapy has been found to be effective when used during laparotomy to improve local control in patients with locally advanced pancreatic cancer. In this study, we report the results of using endoscopic ultrasound- (EUS-)guided interstitial brachytherapy in patients with advanced pancreatic cancer, with respect to tumor response, clinical response, safety, and complications.

Patients And Methods: Fifteen patients (eight men, seven women; median age 61 years) with unresectable pancreatic adenocarcinoma were enrolled into the study, eight patients with stage III disease and seven patients with stage IV disease.

Usefulness of diffusion/perfusion-weighted MRI in patients with non-enhancing supratentorial brain gliomas: a valuable tool to predict tumour grading?

22 patients with non-enhancing supratentorial gliomas on contrast-enhanced MRI underwent both diffusion- and perfusion-weighted MRI (DWI/PWI) before surgical resection. 14 low-grade gliomas (WHO Grade I and II) and 8 anaplastic gliomas were verified histologically. Both apparent diffusion coefficient (ADC) values and relative cerebral blood volume (rCBV) ratios were calculated on the solid portion of the tumour, on peritumoural area, as well as on the contralateral normal white matter, respectively.

Potential of diffusion tensor MRI in the assessment of periventricular leukomalacia.

Aim: To investigate magnetic resonance (MR) diffusion tensor imaging (DTI) and fibre tractography in the assessment of altered major white matter (WM) fibre tracts in periventricular leukomalacia (PVL).

Materials And Methods: Twelve children (male:female = 7:5, age range 3-10 years; mean age = 6.5 years) who had suffered PVL were included in this study.

[Expressions and clinical significance of urokinase-type plasminogen activator (uPA) and uPA receptor (uPAR) in tongue squamous cell carcinoma].

Purpose: To study the expressions and clinical significance of urokinase-type plasminogen activator (uPA) and uPA receptor (uPAR) in tongue squamous cell carcinoma (TSCC).

Methods: The expressions of uPA and uPAR at protein level were examined in 74 cases of TSCC and 15 cases of normal peripheral tissues around cancer (as control) by strep avidin-biotin complex (SABC) immunohistochemical technique. The relationship between the expressions of uPA and uPAR was evaluated by the Spearman's rank correlation test.

[Effects of genistein on the expressions of cell cycle proteins in salivary adenoid cystic carcinoma cell line SACC-83].

Purpose: To investigate the molecular mechanism of cell cycle arrest induced by tyrosine protein kinase inhibitor, genistein, in human salivary adenoid cystic carcinoma cell line SACC-83.

Methods: SACC-83 cells cultured in vitro were treated with genistein, the expressions of CyclinB1, Cdk1, CyclinD1 and Cdk4 proteins were detected with Western blotting, and the results were quantitatively analyzed by FluorChem V2.0 software, statistical analysis was performed with analysis of variance using SPSS11.

[Protective effects of captopril on hyperoxia-induced lung injury in neonatal rats].

Objective: To study the effect of captopril on the histopathology and bronchoalveolar lavage fluid (BALF) in neonatal rats exposed to hyperoxia.

Methods: Forty term neonatal Wistar rats were randomly assigned into Air control, Model, Normal saline control and Captopril-treated groups (n=10 each). The Air control group was exposed to air (FiO2=0.

[Bibliometric analysis of Chinese Journal of Pediatrics].

Objective: To evaluate the quality of articles and the popularity of Chinese Journal of Pediatrics.

Methods: With the database of Chinese Science & Technology Journal Citation Reports 2000-2003, the six indicators, including impact factor (IF), total cited numbers, immediate index, ratio of cited numbers by other journals to total cited numbers, number of citing journals, and cited half-life were evaluated and compared with other Chinese periodicals in pediatrics and related subjects. The cited numbers, distribution of cited areas, and cited institutions of published articles in Chinese Journal of Pediatrics 2000-2003 were analyzed.

[Direct bone morphogenetic protein 2 gene therapy for repairing segmental radial defect in rabbits].

Objective: To study the effect of direct bone morphogenetic protein 2 (BMP-2) gene therapy mediated by adenovirus on repairing bone defect.

Methods: The radial defect models were made on 60 rabbits, which were evenly divided into 4 groups randomly. The 4 groups were treated with different materials: group A, adenovirus carrying BMP-2 gene (Ad-BMP-2) plus bovine cancellous bone (BCB); group B, reconstructed BMP-2 plus BCB; group C, Ad-Lacz plus BCB; and group D, only BCB scaffolds.

[Expression of extracellular matrix metalloproteinase inducer during normal pregnancy].

Objective: To study the expression of extracellular matrix metalloproteinase inducer (EMMPRIN) during normal pregnancy.

Methods: The expression of EMMPRIN was studied with immunohistochemistry in normal pregnancy.

Results: The expression of EMMPRIN was obvious, and appeared mainly on membrane of cytotrophoblast, syncytiotrophoblast, cytotrophoblast column of the villi of gestational weeks 6 to 9, mid-term and term placenta and extravillous cytotrophoblast of deciduas of mid-term and term placenta.

Submucous colon lipoma: a case report and review of the literature.

Colon lipoma is remarkably rare in clinical practice. We reported a case of ascending colon lipoma in an 83-year-old woman. She was asymptomatic with a lipoma of 35 mm x 30 mm x 24 mm in size which was found by routine colonoscopy.

Usefulness of diffusion/perfusion-weighted MRI in rat gliomas: correlation with histopathology.

Rationale And Objectives: Diffusion/perfusion-weighted MRI (DWI/PWI) can provide additional useful information in the diagnosis of patients with brain gliomas in a noninvasive fashion. However, the exact role of these new techniques is still undergoing evaluation. Our hypothesis was that DWI and PWI could be useful for assessment of growth and vascularity of implanted C6 rat gliomas.

[Changes of platelet endothelial cell adhesion molecule-1, tissue type plasminogen activator and plasminogen activator inhibitor-1 expression in the lung tissue of neonatal rats after intraperitoneal injection with lipopolysaccharide].

Objective: To further explore the pathogenesis of neonatal acute lung injury and neonatal pulmonary hemorrhage by establishing the animal model of neonatal acute lung injury (ALI) and by investigating the changes of platelet endothelial cell adhesion molecule-1 (PECAM-1), tissue type plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) in ALI.

Methods: Totally 88 neonatal rats which were divided into 8 groups randomly including one normal saline control group and 30 min, 1 h, 2 h, 4 h, 8 h, 16 h and 24 h post injection groups. The changes of lung pathology in newborn rats were observed at different time after LPS was injected intraperitoneally.