Unraveling prokaryotic diversity distribution and functional pattern on nitrogen and methane cycling in the subtropical Western North Pacific Ocean.

Prokaryotes play an important role in marine nitrogen and methane cycles. However, their community changes and metabolic modifications to the concurrent impact of ocean warming (OW), acidification (OA), deoxygenation (OD), and anthropogenic‑nitrogen-deposition (AND) from the surface to the deep ocean remains unknown. We examined here the amplicon sequencing approach across the surface (0-200 m; SL), intermediate (200-1000 m; IL), and deep layers (1000-2200 m; DL), and characterized the simultaneous impacts of OW, OA, OD, and AND on the Western North Pacific Ocean prokaryotic changes and their functional pattern in nitrogen and methane cycles.

Evaluation of MIC distribution and establishment of epidemiological cut-off values for Streptococcus parauberis isolated from aquatic animals.

Aims: Streptococcus parauberis is responsible for the development of streptococcosis in marine fish. The aim of the current study was to determine the antimicrobial susceptibility of aquatic Strep. parauberis strains, thus establishing laboratory-specific epidemiological cut-off (COWT) values to distinguish wild-type (WT) and nonwild-type (NWT) strains.

Effects of protein oxidation, cathepsins, and various freezing temperatures on the quality of superchilled sturgeon fillets.

Many aquatic products have been stored using superchilling technology, but rarely used for the storage of sturgeon fillets. In this study, we investigated the effects of protein oxidation, cathepsin, and various freezing temperatures on the quality of superchilled sturgeon fillets. Sensory evaluation results showed that the sensory attributes of superchilled (-3 °C) sturgeon fillets were acceptable three times longer (18 days) than samples stored at refrigeration temperatures (4 °C).

Decreases in Concentrations and Human Dietary Intakes of Polychlorinated Biphenyls (PCBs) and Polybrominated Diphenyl Ethers (PBDEs) in Korean Seafood Between 2005 and 2017.

Concentrations of polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) were measured in 23 seafood species widely consumed by the Korean population in the periods of 2005-2007, 2010-2011, and 2015-2017. The ΣPCB (sum of 82 PCB congeners) and ΣPBDE (sum of 19 PBDE congeners) concentrations in the seafood samples of 2015-2017 were 0.06-6.

Antibiotic and heavy-metal resistance of Vibrio parahaemolyticus isolated from oysters in Korea.

Vibrio parahaemolyticus, found frequently in oysters and other seafoods, is the most prevalent gastroenteritis-causing pathogen in Korea and other Asian countries. It is associated exclusively with the consumption of raw or improperly cooked contaminated seafood, especially oysters. In this study, we isolated and characterized 59 V.

Ark shell Scapharca broughtonii hemocyte response against Vibrio anguillarum challenge.

Scapharca broughtonii is one of the most important Arcidae aquaculture species in the Asia-Pacific region. We aimed to investigate the immune responses of hemocytes from ark shell S. broughtonii hemolymph against pathogens.

Optofluidic Single-Cell Genome Amplification of Sub-micron Bacteria in the Ocean Subsurface.

Optofluidic single-cell genome amplification was used to obtain genome sequences from sub-micron cells collected from the euphotic and mesopelagic zones of the northwestern Sargasso Sea. Plankton cells were visually selected and manually sorted with an optical trap, yielding 20 partial genome sequences representing seven bacterial phyla. Two organisms, E01-9C-26 (), represented by four single cell genomes, and Opi.

Occurrence and temporal variation of antibiotic resistance genes (ARGs) in shrimp aquaculture: ARGs dissemination from farming source to reared organisms.

Considerable attention has been paid to the occurrence and abundance of antibiotic resistance genes (ARGs) in aquatic environments. However, the temporal variation and dissemination of ARGs in aquaculture environments and reared organisms need further study. This study investigated the abundance and diversity of ARGs and bacterial community in water source, shrimp pond water, sediment, and shrimps during the rearing period in Pearl River Delta region, South China.

Characterization of Vibrio parahaemolyticus isolated from oysters in Korea: Resistance to various antibiotics and prevalence of virulence genes.

Vibrio parahaemolyticus, found frequently in oysters, is the most prevalent gastroenteritis-causing pathogen in Korea and in several other Asian countries. This study monitored changes in the environmental parameters and occurrence of V. parahaemolyticus in oyster aquaculture sites.

Changes in hemolymph characteristics of ark shell Scapharaca broughtonii dealt with Vibrio anguillarum challenge in vivo and various of anticoagulants in vitro.

The ark shell Scapharca broughtonii is a commercially important shellfish in China. Alserver's solution (AS), modified Alserver's solution (MAS) and Heparin sodium solution (HSS) are common anticoagulants used for shellfish blood. To observe the immune response mediated by its hemocytes, we challenged in vivo S.

Characterisation and expression analysis of two terminal complement components: C7 and C9 from large yellow croaker, Larimichthys crocea.

The large yellow croaker Larimichthys crocea, as one of the most economically important marine fish in China and East Asian countries, are facing the fatal attraction of various pathogens in recent years. Elucidation of the organism immunomodulatory mechanism of croaker response to pathogen infection is essential for the disease control. In present study, we reported for the first time the molecular characterization and expression analysis of two terminal complement components (TCCs) of croaker, Lc-C7 and Lc-C9.

Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from oysters in Korea.

Vibrio parahaemolyticus is the most prevalent gastroenteritis-causing pathogen in Korea and in some other Asian countries. It is frequently found in oysters and other seafood. This study monitored changes in the prevalence of V.

Identification and comparative analysis of the Pseudosciaena crocea microRNA transcriptome response to poly(I:C) infection using a deep sequencing approach.

Two sRNA libraries with or without poly(I:C) infection of large yellow croaker Pseudosciaena crocea were constructed and sequenced using the high-throughput Illumina/Solexa deep sequencing technology. The high-throughput sequencing pipeline yielded 163,79,272 and 217,07,070 raw reads corresponding to 132,27,594 and 206,86,409 clean reads for the normal and infected libraries, respectively. Bioinfromatic analysis identified 534 miRNAs, of which, 158 miRNAs were known in miRBase 20.

Enrichment of omnivorous cercozoan nanoflagellates from coastal Baltic Sea waters.

Free-living nano-sized flagellates are important bacterivores in aquatic habitats. However, some slightly larger forms can also be omnivorous, i.e.

Impact of strontium on skeletal deformities in Baltic cod (Gadus morhua callaris L.).

A study was conducted to examine spinal deformities such as lordosis, scoliosis, and dwarfism in cod (Gadus morhua callaris L.) that were caught in the southern Baltic. The bone tissue of the spine and the muscle of the deformed cod were analyzed for concentration of macroelements (Sr, Ca and P) and toxic metals (Cd, Pb and Hg).

Bioaccumulation and temporal trends of trace elements in flounder from the southern Baltic Sea for the 1996-2003 period.

This study determined the concentrations of copper (Cu), zinc (Zn), cadmium (Cd), lead (Pb), mercury (Hg), and arsenic (As) in flounder from the southern Baltic for the 1996-2003 period. Analysis of more than 200 samples was used to determine the following: (1) concentrations of Hg and As in muscle tissue of flounder from different regions of the southern Baltic; (2) concentrations of Hg in the muscle tissue of flounder of different sizes; and (3) temporal trends in the concentrations of trace metals during the 1996-2003 period. A positive correlation between fish length and Hg concentration was noted.

Variation in fat content and fatty-acid composition of the Baltic herring Clupea harengus membras.

The fat content and fatty-acid profiles of herring, Clupea harengus membras, from the southern Baltic Sea varied depending on when (fishing season) and where (fishing grounds) the fish were caught as well as on their size and sex. The fat, protein and dry matter content and the fatty-acid profiles were assayed in C. h.

Organochlorine pollutants in European eel (Anguilla anguilla L.) from Poland.

The purpose of the current paper is to report the levels of OC pesticides ([summation operator]HCHs, HCB, [summation operator]DDTs), marker PCBs, PCDD/Fs, and dl-PCBs in the muscle tissue of European eel, Anguilla anguilla (L.), collected in the Vistula and Szczecin lagoons (Poland), and in the local market. The results obtained were evaluated according to maximum limits adopted by EU, and some national legislatures.

Natural, social, economical and political influences on fisheries: a review of the transitional area of the Polish waters of the Vistula Lagoon.

A 60 year (1948-2007) dataset gathered by Polish researchers working on the Vistula Lagoon fish assemblages and fisheries has shown this stressed transitional environment to be always been dominated by a few highly abundant fish species. During this period, the surrounding countries Poland and Russia (Kaliningrad) were transformed from centrally-planned economies with fixed prices to free market systems. The organization of the fishery evolved from one in which the majority of the fishing effort was expended by cooperatives, to one which was characterized by individual economic activity.

Does ecological energetics lead to trophic competition in juvenile fish? A case study from the Vistula Lagoon (southern Baltic Sea).

Production, respiration, egestion of faeces and excretion of ammonia were measured in competing early developmental stages of fish species (herring, smelt, perch and stickleback) to calculate their daily energy (food) requirements in the Vistula Lagoon, southern Baltic Sea. The highest consumption (per capita and per unit body mass) was found in perch and European smelt, and the lowest in herring. However, young herring had the highest contribution to the exploitation of food resources because of their dominance in the Lagoon.

Polybrominated diphenyl ethers (PBDEs) in selected fish species from the southern Baltic Sea.

This paper reports concentrations of seven polybrominated diphenyl ether (PBDE) congeners in selected fish species that were collected from the southern Baltic in the 2004-2006 period. Differences in concentrations of PBDEs among the fish species were observed. The mean summation Sigma(7)PBDE concentrations measured in herring (Clupeaharengus), sprat (Sprattus sprattus), and salmon (Salmo salar) samples were 1.

Inter-tissue distribution and evaluation of potential toxicity of PCBs in Baltic cod (Gadus morhua L.).

PCBs measured in the muscle, liver, and gonads of cod collected from two locations in the southern Baltic were evaluated as total (summation operator PCBs), indicator (summation operator PCB(7)), and dioxin-like (summation operator DL-PCBs) concentrations. Gender, tissue lipid content, and collection site were important determinants of PCB levels. Irrespective of collection site, lipid-normalized summation operator PCBs, summation operator PCB(7), and summation operator DL-PCBs in females were the lowest in the muscle, and of similar levels in liver and gonads.

Temporal trends in the bioaccumulation of trace metals in herring, sprat, and cod from the southern Baltic Sea in the 1994-2003 period.

This study is based on raw data obtained from 1 225 samples of herring (Clupea harengus), sprat (Sprattus sprattus), and cod (Gadus morhua) collected in the 1994-2003 period from the Polish coastal zone of the Baltic Sea. This paper presents the results of investigations of the contents of Cu, Zn, Cd, Pb, Hg, and As in fish. The aim was to identify temporal trends in trace metal contamination and variations in the concentrations of Hg in the flesh of herring, sprat, and cod of different lengths.