Cancer mutations rewire the RNA methylation specificity of METTL3-METTL14.

Chemical modification of RNAs is important for posttranscriptional gene regulation. The METTL3-METTL14 complex generates most -methyladenosine (mA) modifications in messenger RNAs (mRNAs), and dysregulated methyltransferase expression has been linked to cancers. Here we show that a changed sequence context for mA can promote oncogenesis.

A national cross-sectional survey of health literacy of caregivers attending Canadian pediatric emergency departments.

Background: Health literacy assessment is key to better meeting family needs and developing informed strategies to promote positive health outcomes for children. The objective of this study was to describe the health literacy of caregivers who use Canadian pediatric emergency departments and relate it to demographic and visit-specific variables.

Methods: This study utilized a descriptive, cross-sectional survey design with medical record review.

Cumaceans (Crustacea, Peracarida) associated with shallow-water hydrothermal vents at Banderas Bay, Mexico.

Background: Cumaceans mostly inhabit marine environments, where they play a crucial role in marine food webs and actively participate in the transfer between benthic and pelagic systems. Scientific interest in these crustaceans has been increasing, but is limited to certain geographic areas, which do not include extreme environments such as hydrothermal vents.

New Information: Therefore, this study aimed to report the distribution of cumaceans in shallow-water hydrothermal vents at Banderas Bay and to identify the specimens present.

Exacerbation of neuropsychiatric symptoms in an adult male with influenza A virus treated with oseltamivir.

Oseltamivir (Tamiflu) package labeling has a warning for neuropsychiatric adverse events (NPAE), most commonly in children and adolescents, especially males. There are several case reports of NPAE in adults treated with oseltamivir, but few document patients with preexisting neuropsychiatric conditions without additional contributing factors. This case report describes a 22-year-old male with a history of bipolar disorder, depression, and attention-deficit/hyperactivity disorder who had been stable on his medication regimen before experiencing sudden worsening of symptoms after the initiation of oseltamivir.

Structural insights into the enzymatic breakdown of azomycin-derived antibiotics by 2-nitroimdazole hydrolase (NnhA).

The antibiotic 2-nitroimidazole (2NI) or azomycin, used for treating drug-resistant tuberculosis and imaging tumor hypoxia, requires activation by bacterial nitroreductases for its antibiotic and cytotoxic effect. Mycobacterium sp. JS330 produces 2-nitroimidazole nitrohydrolase (NnhA) that circumvents 2NI activation, conferring 2NI resistance by hydrolysing it to nitrite and imidazol-2-one (IM2O) instead.

A research protocol to study the critical time window for rehabilitation after incomplete spinal cord injury: early vs. late locomotor training.

Spinal cord injury (SCI) often results in severe motor and sensory deficits, leading to significant disability. Preclinical studies and retrospective studies suggest that a critical window of enhanced neuroplasticity may exist immediately after SCI, during which therapeutic interventions could yield greater functional improvements. The impact of time interval since SCI on efficacy of rehabilitation has not been directly assessed and is the focus of this clinical trial.

Rumination induction task in fMRI: Effects of rumination focused cognitive behavioral therapy and stability in youth.

Background: Rumination is implicated in the onset and maintenance of major depressive disorder (MDD). Rumination-Focused Cognitive Behavioral Therapy (RF-CBT) effectively targets rumination and may change resting-state brain connectivity and change in activation during a rumination induction task (RIT) post-intervention predicts depressive symptoms two years later. We examined brain activation changes during an RIT in adolescents with remitted MDD following RF-CBT and evaluated RIT reliability (or stability) during treatment as usual (TAU).

Decision analysis of Integrated Pest Management: A case study on invasive sea lamprey in the Great Lakes Basin.

Integrated Pest Management (IPM) provides a powerful framework for addressing threats to human well-being caused by nuisance species including invasives. We examined the hypothesis that adaptive management could erode barriers to IPM implementation by developing a decision-analytic adaptive management framework for invasive sea lamprey (Petromyzon marinus) IPM in the Laurentian Great Lakes of North America. The framework addressed objectives associated with coordinating multiple sea lamprey control actions at the regional scale and objectives associated with internal validity of control actions.

Multiconfigurational Electronic Structure of Nickel Cross-Coupling Catalysts Revealed by X-ray Absorption Spectroscopy.

Ni 2,2'-bipyridine complexes are commonly invoked intermediates in metallaphotoredox cross-coupling reactions. Despite their ubiquity, design principles targeting improved catalytic performance remain underdetermined. A series of Ni(bpy)(Ar)Cl (R = MeOOC, -Bu, R' = CH, CF) complexes were proposed to have multiconfigurational electronic structures on the basis of multiconfigurational/multireference calculations, with significant mixing of Ni → bpy metal-to-ligand charge transfer (MLCT) configurations into the ground-state wave function.

Circulating cytokine associations with clinical outcomes in melanoma patients treated with combination nivolumab plus ipilimumab.

Nivolumab plus ipilimumab (aCTLA-4/aPD-1) combination therapy has significantly improved clinical outcomes in patients with metastatic melanoma, with 50%-60% of patients responding to treatment, but predictors of response are poorly characterized. We hypothesized that circulating cytokines and peripheral white blood cells may predict response to therapy and evaluated 15 cytokines and complete blood counts (CBC with differentials) from 89 patients with advanced melanoma treated with combination therapy from three points in time: pre-treatment, one month and approximately three months after starting therapy. Clinical endpoints evaluated included durable clinical benefit (DCB), progression-free survival (PFS), and overall survival (OS).

Impact of Intracellular Proteins on μ-Opioid Receptor Structure and Ligand Binding.

Chronic pain is a prevalent problem affecting approximately one out of every five adults in the U.S. The most effective way to treat chronic pain is with opioids, but they cause dangerous side effects such as tolerance, addiction, and respiratory depression, which makes them quite deadly.

Prevalence of participation of Brazilian aged people in Advanced Activities of Daily Living and associated factors.

Objective: To estimate the prevalence of participation of aged Brazilians in Advanced Activities of Daily Living and associated factors.

Methods: Cross-sectional study developed with secondary data from the National Health Survey. Aged people (60 years old or older) were included.

Optimising aerial herbicide treatment for control of dense conifer infestations: a New Zealand case study.

Background: Conifers, mainly of the genus Pinus, have invaded natural and productive landscapes worldwide. Controlling dense conifer invasions is challenging and requires both effective initial control methods and long-term site management to prevent re-invasion. Broadcast aerial herbicide application is often the preferred treatment method for dense infestations of pines with high rates of complex herbicide mixtures used.

Visual Field Asymmetries in Responses to ON and OFF Pathway Biasing Stimuli.

Recent reports suggest the ON and OFF pathways are differentially susceptible to selective vision loss in glaucoma. Thus, perimetric assessment of ON- and OFF-pathway function may serve as a useful diagnostic. However, this necessitates a developed understanding of normal ON/OFF pathway function around the visual field and as a function of input intensity.

Development of a new Peyronie's disease self-assessment screening app.

Peyronie's disease (PD) is an underdiagnosed and undertreated fibroproliferative disorder associated with the formation of plaques that results in penile curvature and discourages some men from seeking medical consultation. The PD self-assessment app, a digital application (app), was developed to increase PD awareness, provide men with a self-screening and educational tool for PD, and help eliminate barriers to seeking consultation with a physician. Prior to deployment, 4 board-certified urologists provided qualitative reviews on the app's usability and utility as a screening tool.

Does Chronic Kidney Disease Influence Revascularization Strategy After Acute Coronary Syndrome? A Systematic Review and Meta-Analysis.

Background: Coronary artery bypass grafting (CABG) provides superior long-term outcomes to percutaneous coronary intervention (PCI) for complex multivessel coronary artery disease (CAD). People with chronic kidney disease (CKD) have increased prevalence of multivessel CAD, but also increased surgical risk. We investigated whether CKD predicted real-world use of CABG, versus PCI, in patients revascularized for acute coronary syndrome (ACS).

Identification and odor exposure regulation of odorant-binding proteins in .

The highly developed sensitive olfactory system is essential for Scott (Hemiptera: Pentatomidae) adults, an widely distributed natural predatory enemy, to locate host plants. During this process, odorant-binding proteins (OBPs) are thought to have significant involvement in the olfactory recognition. However, the roles of OBPs in the olfactory perception of are not frequently reported.

A prognostic model for predicting functional impairment in youth mental health services.

Background: Functional impairment is a major concern among those presenting to youth mental health services and can have a profound impact on long-term outcomes. Early recognition and prevention for those at risk of functional impairment is essential to guide effective youth mental health care. Yet, identifying those at risk is challenging and impacts the appropriate allocation of indicated prevention and early intervention strategies.

Quantifying Similarities Between MediaPipe and a Known Standard to Address Issues in Tracking 2D Upper Limb Trajectories: Proof of Concept Study.

Background: Markerless motion tracking methods have promise for use in a range of domains, including clinical settings where traditional marker-based systems for human pose estimation are not feasible. Artificial intelligence (AI)-based systems can offer a markerless, lightweight approach to motion capture. However, the accuracy of such systems, such as MediaPipe, for tracking fine upper limb movements involving the hand has not been explored.