Comparative Analysis of Mitogenomes in Leafhopper Tribe Deltocephalini (Hemiptera: Cicadellidae: Deltocephalinae): Structural Conservatism and Phylogeny.

Previous studies on the gene order and composition of leafhopper mitochondrial genomes have revealed a high level of conservation in overall genome structure. Some members of Deltocephalinae, the largest subfamily, exhibit tRNA gene rearrangements; however, few mitogenomes have been sequenced in this group and the degree of structural variation within tribes remains unclear. In this study, we sequenced the complete mitogenomes of 14 species belonging to four genera of tribe Deltocephalini from China and compared them with the two previously reported mitogenomes for this tribe.

Glycosyltransferase-Mediated Modulation of Reactive Oxygen Species Enhances Non-host Resistance to Pst DC3000 in Nicotiana benthamiana.

Non-host resistance (NHR) governs defense responses against a broad range of potential pathogen species in contrast with host resistance. To identify specific genes involved in disease resistance, we used a virus-induced gene-silencing screen in Nicotiana benthamiana and identified glycosyltransferase (NbGT) as an essential component of NHR. NbGT silencing enhanced the hypersensitivity response, reactive oxygen species response, and callose deposition in N.

Taxonomic Revision of Ningshan Odd-Scaled Snake, (Serpentes, Xenodermidae), with Description of a New Subspecies from Western China.

(Yang, Huang, Jiang, Burbrink, and Huang, 2022) was first described in Ningshan County, Shaanxi Province, China in 2022, based on seven female specimens. In this study, based on phylogenetic analyses using mitochondrial 12S ribosomal RNA (), 16S ribosomal RNA (), cytochrome c oxidase subunit 1 (), cytochrome b (cyt ) gene fragments, and morphological examinations of specimens, we revise the taxonomic status of , and provide additional data on this species. The molecular phylogeny indicated that is nested in a highly supported monophyletic group, forming a sister taxon to , and is divided into two well-supported lineages, A and B, with an uncorrected -distance between lineages from 3.

Characterization and complete genome sequence of a novel zetapatitivirus from Penicillium exsudans isolate RCEF7900.

Penicillium exsudans strain RCEF7900, obtained from leaf litter, was found to harbor two double-stranded RNA (dsRNA) elements, designated as dsRNA1 and dsRNA2, with lengths of 1,816 bp and 1,625 bp, respectively. dsRNA1 encodes a 572-amino-acid RNA-dependent RNA polymerase (RdRp) protein (65.29 kDa), while dsRNA2 encodes a 503-amino-acid coat protein (CP) (56.

Non-Radical Mediated Photocatalytic HO Synthesis in Conjugate-Enhanced Phenolic Resins with Ultrafast Charge Separation.

It is essential for the development of highly efficient polymeric photocatalysts for hydrogen peroxide (HO) production. Nevertheless, the non-uniform molecular structures and sluggish reaction pathway of polymeric photocatalysts lead to low conversion efficiency. In this work, we report sulfur-contained phenolic resins with regulated conjugation for photocatalytic HO production.

A new species of Isocapnia (Plecoptera: Capniidae) from China, with notes on I. apicispina Chen & Du, 2017 and I. arcuata Zhiltzova, 1975.

This study describes and illustrates a new species of Isocapnia Banks, 1938, I. niuerheensis Yang & Du, sp. nov.

Identification and pharmacological characterization of pH-sensitive chloride channels in the fall armyworm, Spodoptera frugiperda.

The pH-sensitive chloride channels (pHCls) are unique to invertebrates and play crucial roles in fluid regulation, food selection, and intake. In this study, we identified and isolated two cDNAs encoding the SfpHCl1 and SfpHCl2 subunits from the fall armyworm, Spodoptera frugiperda. Both subunits exhibited similar expression patterns.

Mechanistic exploration of odorant binding protein-mediated chlorpyrifos resistance in Nilaparvata lugens: Insights from insecticide sequestration and transcriptional regulation.

The effectiveness and sustainable application of insecticides are severely threatened by the rapid evolution of resistance in agricultural pests. Recent research indicates that odorant binding proteins (OBPs) may be involved in facilitating insecticide resistance, while the specific mechanisms remain poorly understood. Herein, 11 OBPs were identified from Nilaparvata lugens.

The Forgotten Tobamovirus Genes Encoding the 54 kDa Protein and the 4-6 kDa Proteins.

This article reviews the literature concerning the largely forgotten tobamovirus gene products for which no functions have been ascribed. One of these gene products is the 54 kDa protein, representing the RNA-dependent RNA polymerase segment of the 183 kDa protein translated from the I-subgenomic mRNA, but which has been found only by in vitro translation and not . The other is a collection of small proteins, expressed from alternative reading frames (likely from internal ribosome entry sites) in either or both the gene or the gene.

Isolation and Evaluation of Indigenous Isolates of and Synergistic Control of with the Parasitoid .

Entomopathogenic fungi (EPFs) are capable of infecting a variety of insect pests and are widely used as biopesticides worldwide. This study intended to isolate indigenous EPFs from cadavers of and investigate their effects on the fall armyworm (FAW), a globally widespread invasive pest. Morphological and molecular analyses confirmed four native EPF strains all belong to .

Discovery and characterization of a novel Lepidoptera-specific antimicrobial peptide from the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae).

Antimicrobial peptides (AMPs) are critical components of innate immunity in diverse organisms, including plants, vertebrates, and insects. This study identified and characterized a novel Lepidoptera-specific AMP, named lepidoptin, from the invasive pest Spodoptera frugiperda (Lepidoptera: Noctuidae). Lepidoptin is a 116-amino acid protein containing a signal peptide and a novel β-sandwich domain that is distinct from previously reported AMPs.

A new species of (Plecoptera, Leuctridae) from Yunnan Province, China.

Background: A new species of the genus (Plecoptera, Leuctridae) from Yunnan Province, China, , is described and illustrated.

New Information: This new species is compared to the similar species, (Wu, 1949). Additionally, we provide a supplemental description and colour plates of .

Electron-Donor-Mediated Divergent Transformation of Br-R via EDA Complex for the Synthesis of Fluorine-Containing Oxindoles and Amides.

We have developed an unprecedented electron-donor-controlled divergent reaction between -alkylsulfonylated acrylamides and Br-R, facilitated by an electron donor-acceptor (EDA) complex, for the synthesis of fluorine-containing oxindoles and amides. Key to this divergent transformation is the EDA complex generation by altering electron donors. This approach has several advantages, such as broad substrate scope, being metal-catalyst- and photocatalyst-free, and having good functional group tolerance.

Study on the Mechanism of Polysaccharide in Reducing Depression-like Behavior in Mice.

Background/objectives: Depression is a prevalent worldwide mental health disorder that inflicts significant harm to individuals and society. is an edible fungus that contains a variety of nutrients, including polysaccharides. This study aims to investigate the monosaccharide composition and molecular weight of the polysaccharide (DDP-B1), followed by an exploration of its antidepressant effects in chronic unpredictable mild stress (CUMS) mice.

Implications of inhalation bioaccessibility for the exposure assessment of drifting airborne pesticides caused by field spraying.

Pesticide contamination in ambient air due to spray drifting has received extensive attention. Quantifying the associated health risk highlights the importance of incorporating bioaccessibility into inhalation exposure assessments rather than using the total inhaled concentration of airborne pesticides. In this study, we measured the inhalation unit exposure (UE) of three typical pesticides (lambda-cyhalothrin, phoxim, and acetamiprid) during application and post-application drift at the recommended application dosage.

Viroids and Satellites and Their Vector Interactions.

Many diseases of unknown etiology with symptoms like those caused by plant viruses but for which no virions could be found were described during the early and mid-20th century [...

Comparative Mitogenomics Analysis Revealed Evolutionary Divergence among Species and Gene Arrangement and Intron Dynamics of Ophiocordycipitaceae.

The species of are cosmopolitan and multitrophic fungi that can infect a wide range of invertebrate hosts. This study reports the mitogenome of , a specialized spider pathogenic fungus. The 112,465 bp mitogenome encoded genes typically found in fungal mitogenomes, and a total of 52 introns inserted into seven genes.

Identification and validation of reference genes for RT-qPCR analysis in (Hymenoptera: Bethylidae).

Gene expression studies in organisms are often conducted using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR), and the accuracy of RT-qPCR results relies on the stability of reference genes. We examined ten candidate reference genes in , a parasitoid wasp that is a natural enemy of long-horned beetle pests in forestry, including and , regarding variable biotic and abiotic factors such as body part, life stage, hormone, diet, and temperature. Data were analysed using four dedicated algorithms (ΔCt, BestKeeper, NormFinder, and geNorm) and one comparative tool (RefFinder).

The SUMO gene MrSmt3 is involved in SUMOylation, conidiation and stress response in Metarhizium robertsii.

Smt3, as a small ubiquitin-like modifier (SUMO), play an essential role in the regulation of protein SUMOylation, and thus this process can affect various important biological functions. Here, we investigated the roles of MrSmt3 (yeast SUMO/Smt3 homologs) in the entomopathogenic fungus Metarhizium robertsii. Our results of subcellular localization assays demonstrated that MrSmt3 was present in the cytoplasm and nucleus, whereas MrSmt3 was largely localized in the nucleus during oxidative stress.

Design, Synthesis, and Antifungal Evaluation of Novel Pyrazole-5-sulfonamide Derivatives for Plant Protection.

To develop further novel environmentally friendly antifungal agents with high efficacy, a series of pyrazole-5-sulfonamide derivatives were designed and synthesized by using the active molecules synthesized in previous works as lead compounds. Their antifungal activities were evaluated in vitro against ten highly destructive plant pathogenic fungi. The bioassay results indicated that more than half of the target compounds displayed potent antifungal activities (inhibition rate ≥85%) against and at 20 mg/L.

The growth-blocking peptide is a dual regulator of development and immunity with biocontrol potential in Spodoptera frugiperda (Lepidoptera: Noctuidae).

Insect growth-blocking peptides (GBPs) are a family of cytokines found in several insect orders and are known for their roles in regulating development, paralysis, cell proliferation, and immune responses. Despite their diverse functions, the potential of GBPs as biocontrol targets against the pest Spodoptera frugiperda (Lepidoptera: Noctuidae) has not been fully explored. In this study, S.

Zeta class glutathione S-transferase is involved in phoxim tolerance and is potentially regulated by the transcription factor CncC in Agrotis ipsilon (Lepidoptera: Noctuidae).

The black cutworm, Agrotis ipsilon (Lepidoptera: Noctuidae), is an important agricultural pest. Phoxim is an organophosphate insecticide that has been widely used to control A. ipsilon.