Influence of mosaicism on sexing of human preembryos detected by the polymerase chain reaction.

Purpose: Preimplantation sex determination using a single cell by the polymerase chain reaction (PCR) was investigated to elucidate the influence of mosaicism.

Methods: The SRY and ZFX genes were coamplified as target sequences for the Y and X chromosomes, respectively. The sensitivity of the single and nested PCR method was examined initially followed by amplification of single amniocytes by the nested PCR.

[Depression in the field of internal medicine].

Masked depression is a common case of depression observed in the field of internal medicine. Masked depression redeems the organic disease alone, and so is often detected in the case of unidentified complaints. Symptomatic depression associated with the underlying disease is another type of depression that is commonly observed.

[Etiological study of spinal ligament ossification with special reference to dietary habits and serum sex hormones].

We studied the relationship between dietary habits and the ossification of the posterior longitudinal ligaments (OPLL) in 55 patients. We analyzed carbon and nitrogen isotope ratios of the patients' hair in order to measure the intake of various types of food. Two sets of questionnaire on the patients' food preference and the intake are also analyzed.

[Occupational cancer of the urinary bladder: the diagnostic value of urinary cytology in dyestuff workers exposed to aromatic amines].

A total of 90 dyestuff workers who were engaged in the production of aromatic amines during the post-war period after the 2nd world war have been examined since 1967 by exfoliative cytologic examination. Those identified as class-3 or over by this first screening test were then submitted to a second screening which consisted of periodic cystoscopic examination and 1 or 2 intravenous pyelographies per year. This system of mass screening has been used for the past 20 years, and we have discovered 23 cases of bladder tumor, a case of asynchronous bilateral ureteral tumor and a case of ureteral tumor.