Selenium induces mercury isotope fractionation and detoxification in marine fish.

Prey fish are vital in transferring nutrients and contaminants from lower to higher trophic levels. Although the role of selenium (Se) on mercury (Hg) detoxification has been identified in marine mammals and seabirds, the metabolic pathways of Hg-Se interaction in fish remain unknown. We used Hg stable isotopes (δ202Hg, Δ199Hg) to characterize the internal dynamics of methylmercury (MeHg) and inorganic Hg (IHg) in the presence and absence of Se in juvenile olive flounders (Paralichthys olivaceus).

A novel ddPCR™ assay for eDNA detection and quantification of Greater Amberjack and three congeners in US waters: challenges and application to fisheries independent surveys.

Background: Four species support recreational and commercial fisheries along the U.S. Atlantic Ocean and the Gulf of Mexico, with the Gulf of Mexico stock being overfished for over three decades.

Fish gut symbiotic bacterium Bacillus thuringiensis: RSM optimization for its extracellular lipase enzyme production, lipase-protein purification, characterization, and docking analysis.

Lipase enzymes play a vital role in digestion and nutrient metabolism in host organisms, with symbiotic bacteria producing abundant enzymes, carbohydrates, vitamins, and other nutrients. This study aimed to isolate, identify, and screen lipase-producing bacteria from the gut of Systomus sarana, optimize enzyme production using Response Surface Methodology (RSM), and characterize the extracted lipase protein. A total of 11 bacterial strains were isolated and identified through 16S rRNA sequencing.

Tomato B-cell lymphoma2 (Bcl2)-associated athanogene 5 (SlBAG5) contributes negatively to immunity against necrotrophic fungus Botrytis cinerea through interacting with SlBAP1 and modulating catalase activity.

The evolutionarily conserved and multifunctional B-cell lymphoma2 (Bcl2)-associated athanogene proteins (BAGs), serving as co-chaperone regulators, play a pivotal role in orchestrating plant stress responses. In this study, the possible involvement of tomato SlBAG genes in resistance to Botrytis cinerea was examined. The SlBAG genes respond with different expression change patterns to B.

Historical biogeography of the Mugil cephalus species complex and its rapid global colonization.

Our understanding of speciation processes in marine environments remains very limited and the role of different reproductive barriers are still debated. While physical barriers were considered important drivers causing reproductive isolation, recent studies highlight the importance of climatic and hydrological changes creating unsuitable habitat conditions as factors promoting population isolation. Although speciation in marine fishes has been investigated from different perspectives, these studies often have a limited geographical extant.

Non-coding RNAs and regulatory networks involved in the Ameson portunus (Microsporidia)-Portunus trituberculatus interaction.

Ameson portunus, the causative agent of "toothpaste disease" in Portunus trituberculatus and "slurry-like syndrome" in Scylla paramamosain, has resulted in considerable economic losses in the marine crab aquaculture industry in China. Practical control strategies are yet unavailable. Non-coding RNAs (ncRNAs) are crucial components of gene regulation of intracellular parasites, however, their roles in regulating the microsporidia-host interaction remain limited.

Effects of water ammonia nitrogen on hemolymph and intestinal microbiota of Litopenaeus vannamei.

Ammonia stress was detrimental to shrimp, but how water ammonia nitrogen (ammonia-N) influences the shrimp's health remains unclear. Thus, this study was designed to investigate the effects of water ammonia-N on hemolymph ammonia-N concentration, hepatopancreas structure, and the intestinal microbiota of Litopenaeus vannamei with four experiments. We found that the concentration of ammonia-N in shrimp hemolymph was significantly higher than that in pond water, indicating that water ammonia-N stimulates the accumulation of hemolymph ammonia-N.

The impact of bisphenol A on gill health: A focus on mitochondrial dysfunction induced disorders of energy metabolism and apoptosis in Meretrix petechialis.

Bisphenol A (BPA), a well-known chemical compound used in various daily goods, has been associated with adverse effects on animal metabolic processes. However, the specific impacts of BPA exposure on clam gills remain largely unexplored. To investigate the effects of BPA on energy metabolism and apoptosis in Meretrix petechialis gills, clams were exposed to varying concentrations of BPA (1, 10, and 100 μg/L) for 21 days.

Fatty Acid Profiles Linked to Organohalogen Exposure in Cetaceans from the Northern South China Sea.

Increasing evidence suggests that organohalogen contaminants (OHCs) could disrupt lipid metabolism in organisms, prompting consideration of fatty acids (FAs) as biological tools for assessing chemical stress in biological systems. This study examined 87 OHCs and 32 FAs in two sentinel cetacean species─Indo-Pacific humpback dolphins ( = 128) and Indo-Pacific finless porpoises ( = 26)─from the northern South China Sea (NSCS), a global hotspot for OHCs. Our results revealed higher OHC levels in these cetaceans than global averages.

Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication.

Interferon regulatory factor 7 (IRF7)-mediated type I interferon antiviral response is crucial for regulating the host following viral infection in chickens. Infectious bursal disease virus (IBDV) is a double-stranded RNA virus that induces immune suppression and high mortality rates in chickens aged 3-6 weeks. Previous studies have shown that IBDV infection antagonizes the type I interferon production to facilitate viral replication in the cell, and IRF7 signaling might play an important role.

An Au-Ag@Au fiber surface plasmon resonance sensor for highly sensitive detection of fluoroquinolone residues.

Antibiotic residue detection plays an important role in protecting human health, but real-time, rapid, and highly sensitive detection is still challenging. Herein, gold and silver nanoparticles (Au-Ag NPs) were grown on the surface of optical fibers and a 50 nm thick gold film was deposited on the sensor's surface to fabricate the Au-Ag@Au fiber SPR sensor. The sensitivity of the sensor reached 3512 nm per RIU in the refractive index range of 1.

Warming-driven migration of enterotypes mediates host health and disease statuses in ectotherm Litopenaeus vannamei.

Global warming has threatened all-rounded hierarchical biosphere by reconstructing eco-structure and bringing biodiversity variations. Pacific white shrimp, a successful model of worldwide utilizing marine ectothermic resources, is facing huge losses due to multiple diseases relevant to intestinal microbiota (IM) dysbiosis during temperature fluctuation. However, how warming mediates shrimp health remains poorly understood.

Temperature seasonality regulates organic carbon burial in lake.

Organic carbon burial (OCB) in lakes, a critical component of the global carbon cycle, surpasses that in oceans, yet its response to global warming and associated feedbacks remains poorly understood. Using a well-dated biomarker sequence from the southern Tibetan Plateau and a comprehensive analysis of Holocene total organic carbon variations in lakes across the region, here we demonstrate that lake OCB significantly declined throughout the Holocene, closely linked to changes in temperature seasonality. Process-based land surface model simulations clarified the key impact of temperature seasonality on OCB in lakes: increased seasonality in the early Holocene saw warmer summers enhancing ecosystem productivity and organic matter deposition, while cooler winters improved organic matter preservation.

The differential physiological responses to heat stress in the scleractinian coral Pocillopora damicornis are affected by its energy reserve.

The scleractinian corals conduct various responses upon heat stress such as bleaching and tissue loss, and colonies from the same coral species can conduct differential physiological activities with the biochemical basis unknown. In the present study, factors that influence the heat stress responses in coral Pocillopora damicornis were investigated. It was observed that P.

A vision model for automated frozen tuna processing.

Accurate and rapid segmentation of key parts of frozen tuna, along with precise pose estimation, is crucial for automated processing. However, challenges such as size differences and indistinct features of tuna parts, as well as the complexity of determining fish poses in multi-fish scenarios, hinder this process. To address these issues, this paper introduces TunaVision, a vision model based on YOLOv8 designed for automated tuna processing.

Unveiling triclosan biodegradation: Novel metabolic pathways, genomic insights, and global environmental adaptability of Pseudomonas sp. strain W03.

The polychlorinated aromatic antimicrobial agent triclosan (TCS) is widely used to indiscriminately and rapidly kill microorganisms. The global use of TCS has led to widespread environmental contamination, posing significant threats to ecosystem and human health. Here we reported a newly isolated Pseudomonas sp.

Effects of Cu(Ⅱ) stress on embryonic development, oxidative stress response, cholinergic system and energy metabolism in the Sepiella maindroni.

In order to investigate the causes of population degradation and resource decline, this thesis investigated the ecotoxicological effects of heavy metal Cu(Ⅱ) on the embryonic development of Sepiella maindroni. Results indicate significant effects of Cu(Ⅱ) concentrations on the developmental toxicity, teratogenicity, and lethality of S. maindroni embryos.

Molecular dynamics and factors governing recalcitrance of dissolved organic matter: Insights from laboratory incubation and ultra-high resolution mass spectrometry.

The oceanic dissolved organic matter (DOM) reservoir is one of Earth's largest carbon pools, yet the factors contributing to its recalcitrance and persistence remain poorly understood. Here, we employed ultra-high resolution mass spectrometry (UHRMS) to examine the molecular dynamics of DOM from terrestrial, marine and mixed sources during bio-incubation over weekly, monthly, and one year time spans. Using Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), we classified DOM into three distinct categories (Consumed, Resistant and Product) based on their presence or absence at the start and end of the incubation.

Genomic Insight into Isolates from Fresh Raw Mussels and Ready-to-Eat Stuffed Mussels.

Consuming raw or undercooked mussels can lead to gastroenteritis and septicemia due to contamination. This study analyzed the prevalence, density, species diversity, and molecular traits of spp. in 48 fresh raw wild mussels (FRMs) and 48 ready-to-eat stuffed mussels (RTE-SMs) through genome analysis, assessing health risks.

Transcriptomic Insights into Post-Spawning Death and Muscle Atrophy in Ayu ().

In semelparous species like the ayu (), spawning is followed by rapid physiological decline and death; yet, the underlying molecular mechanisms remain largely unexplored. This study examines transcriptomic changes in ayu skeletal muscle before and after spawning, with a focus on key genes and pathways contributing to muscle atrophy and metabolic dysfunction. Through RNA sequencing and DEG analysis, we identified over 3000 DEGs, and GSEA and KEGG pathway analysis revealed significant downregulation of energy metabolism and protein degradation.

Complete Mitochondrial Genome of (Perciformes: Niphonidae): Genome Characterization and Phylogenetic Analysis.

The species (Cuvier, 1829) is the only representative of the family Niphonidae and the genus , and its taxonomic history is complicated; it is still unclear in a phylogenetic sense. In this study, we report the complete mitochondrial genome of (OP391482), which was determined to be 16,503 bp long with biased A + T contents (53.8%) using next-generation technology.

Comparative analysis of machine learning approaches for predicting the risk of vaginal laxity.

This study develops predictive models for Chinese female patients with VL utilizing machine learning techniques. The aim is to create an effective model that can assist in clinical diagnosis and treatment of vaginal relaxation, thereby enhancing women's pelvic floor health. In total, 1184 women with VL have been randomly selected and categorized into groups using the finger measurement method.

Complete genome sequence of the marine mangrove fungus Sarcopodium sp.QM3-1 confirmed its high potential for antimicrobial activity.

Mangroves, owing to their unique living environment, serve as an important source of natural bioactive compounds. Sarcopodium sp. QM3-1, a marine fungus isolated from mangrove sediments of Quanzhou Bay, exhibited antifungal activity against the plant pathogen Agrobacterium tumefaciens and Magnaporthe oryzae.

Salidroside production through cascade biocatalysis with a thermostability-enhanced UDP-glycosyltransferase.

Salidroside is a phenylpropanoid glycoside with wide applications in the food, pharmaceutical, and cosmetic industries; however, the plant genus Rhodiola, the natural source of salidroside, has slow growth and limited distribution. In this study, we designed a novel six-enzyme biocatalytic cascade for the efficient production of salidroside, utilizing cost-effective bio-based L-Tyrosine as the starting material. A preliminary analysis revealed that the poor thermostability of the Bacillus licheniformis UDP-glycosyltransferase (EC 2.

Keystone bacterial groups dominate Escherichia coli O157:H7 survival in long-term reclaimed water headwater stream.

Escherichia coli (E. coli) O157:H7 is a highly pathogenic zoonotic bacterium, with water serving as a key medium for its environmental transmission. However, the survival characteristics of E.